4123-0385

3-ethyl-2-[(E)-2-(5-nitrofuran-2-yl)ethenyl]quinazolin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_550
Screen concentration 25.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 5731123
SMILES CCN1C(=NC2=CC=CC=C2C1=O)C=CC3=CC=C(O3)[N+](=O)[O-]
Standardized SMILES CCN1C(=Nc2ccccc2C1=O)C=Cc3oc(cc3)[N+](=O)[O-]
Molecular weight 311.2921
ALogP 2.93
H-bond donor count 0
H-bond acceptor count 5
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.64
% growth inhibition (Hom. pool) 6.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5731123
Download HIP data (tab-delimited text)  (excel)
Gene:FAF1(YIL019W)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:GPI19(YDR437W)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.07||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:HYM1(YKL189W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:PRP22(YER013W)|FD-Score:3.87|P-value:5.52E-5|Clearance:0.09||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RPL10(YLR075W)|FD-Score:-3.92|P-value:4.41E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN5(YDL147W)|FD-Score:3.77|P-value:8.10E-5|Clearance:0.61||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP0(YLR340W)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.06||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:3.95|P-value:3.90E-5|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:4.52|P-value:3.07E-6|Clearance:0.13||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RPT3(YDR394W)|FD-Score:6.56|P-value:2.67E-11|Clearance:1.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:5.35|P-value:4.40E-8|Clearance:0.8||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SEC31(YDL195W)|FD-Score:4.39|P-value:5.54E-6|Clearance:0.44||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC4(YFL005W)|FD-Score:3.91|P-value:4.56E-5|Clearance:0.01||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SPC98(YNL126W)|FD-Score:5.5|P-value:1.87E-8|Clearance:0.15||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TOA1(YOR194C)|FD-Score:3.9|P-value:4.80E-5|Clearance:0.03||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YJL086C(YJL086C_d)|FD-Score:4.55|P-value:2.74E-6|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:FAF1(YIL019W)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:GPI19(YDR437W)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.07||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:HYM1(YKL189W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:PRP22(YER013W)|FD-Score:3.87|P-value:5.52E-5|Clearance:0.09||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RPL10(YLR075W)|FD-Score:-3.92|P-value:4.41E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPN5(YDL147W)|FD-Score:3.77|P-value:8.10E-5|Clearance:0.61||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPP0(YLR340W)|FD-Score:3.09|P-value:9.96E-4|Clearance:0.06||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:3.95|P-value:3.90E-5|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:4.52|P-value:3.07E-6|Clearance:0.13||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RPT3(YDR394W)|FD-Score:6.56|P-value:2.67E-11|Clearance:1.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT4(YOR259C)|FD-Score:5.35|P-value:4.40E-8|Clearance:0.8||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SEC31(YDL195W)|FD-Score:4.39|P-value:5.54E-6|Clearance:0.44||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC4(YFL005W)|FD-Score:3.91|P-value:4.56E-5|Clearance:0.01||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SPC98(YNL126W)|FD-Score:5.5|P-value:1.87E-8|Clearance:0.15||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TOA1(YOR194C)|FD-Score:3.9|P-value:4.80E-5|Clearance:0.03||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YJL086C(YJL086C_d)|FD-Score:4.55|P-value:2.74E-6|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5731123
Download HOP data (tab-delimited text)  (excel)
Gene:ATG1(YGL180W)|FD-Score:6.07|P-value:6.22E-10||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATO2(YNR002C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:ATP17(YDR377W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AZF1(YOR113W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BUD17(YNR027W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CCS1(YMR038C)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:5.04|P-value:2.38E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COX9(YDL067C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYC7(YEL039C)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:CYS4(YGR155W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL4(YIR028W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP1(YPL170W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DDR2(YOL052C-A)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DPH5(YLR172C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:FYV5(YCL058C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAD1(YMR250W)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GIP1(YBR045C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GLE2(YER107C)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLO1(YML004C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:HIT1(YJR055W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HST2(YPL015C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IMG2(YCR071C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC8(YJL051W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:KES1(YPL145C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LCB5(YLR260W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LIF1(YGL090W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LOC1(YFR001W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LRS4(YDR439W)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LTE1(YAL024C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS12(YIL094C)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MCH5(YOR306C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MDY2(YOL111C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF1(YLR069C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MMS2(YGL087C)|FD-Score:5.34|P-value:4.58E-8||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MUD1(YBR119W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:OCT1(YKL134C)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI3(YJR073C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OSH6(YKR003W)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PAT1(YCR077C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PCL8(YPL219W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PHB2(YGR231C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PPS1(YBR276C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD18(YCR066W)|FD-Score:11.9|P-value:6.15E-33||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:7.97|P-value:7.83E-16||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:8.52|P-value:7.93E-18||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RBK1(YCR036W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative ribokinase Gene:RGL1(YPL066W)|FD-Score:5.02|P-value:2.54E-7||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RHB1(YCR027C)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RML2(YEL050C)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPH1(YER169W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RUP1(YOR138C)|FD-Score:-3.82|P-value:6.63E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:RXT3(YDL076C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SGT2(YOR007C)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:STM1(YLR150W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TRM11(YOL124C)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TSC3(YBR058C-A)|FD-Score:19.5|P-value:5.67E-85||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBC13(YDR092W)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:URM1(YIL008W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:URN1(YPR152C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VPH2(YKL119C)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS21(YOR089C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS38(YLR360W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:VTC2(YFL004W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YEA6(YEL006W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER119C-A(YER119C-A_d)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR161W-C(YGR161W-C_p)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YGR259C(YGR259C_d)|FD-Score:3.8|P-value:7.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YJL055W(YJL055W_p)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YJL119C(YJL119C_d)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR037W(YJR037W_d)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKR073C(YKR073C_d)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR040C(YLR040C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YNL162W-A(YNL162W-A_p)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNL226W(YNL226W_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL024W(YOL024W_p)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPR063C(YPR063C_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:ER-localized protein of unknown function Gene:YPT35(YHR105W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT53(YNL093W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin Gene:ATG1(YGL180W)|FD-Score:6.07|P-value:6.22E-10||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATO2(YNR002C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:ATP17(YDR377W)|FD-Score:5.11|P-value:1.64E-7||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AZF1(YOR113W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:BUD17(YNR027W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CCS1(YMR038C)|FD-Score:3.93|P-value:4.32E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC26(YFR036W)|FD-Score:5.04|P-value:2.38E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COX9(YDL067C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYC7(YEL039C)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:CYS4(YGR155W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL4(YIR028W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAP1(YPL170W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DDR2(YOL052C-A)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DPH5(YLR172C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm Gene:FYV5(YCL058C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAD1(YMR250W)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GIP1(YBR045C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GLE2(YER107C)|FD-Score:4.9|P-value:4.91E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLO1(YML004C)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:HIT1(YJR055W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HST2(YPL015C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IMG2(YCR071C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC8(YJL051W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:KES1(YPL145C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LCB5(YLR260W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LIF1(YGL090W)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LOC1(YFR001W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LRS4(YDR439W)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LTE1(YAL024C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:LYS12(YIL094C)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MCH5(YOR306C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MDY2(YOL111C)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEF1(YLR069C)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MMS2(YGL087C)|FD-Score:5.34|P-value:4.58E-8||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MUD1(YBR119W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:OCT1(YKL134C)|FD-Score:-4.14|P-value:1.74E-5||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI3(YJR073C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OPI9(YLR338W_d)|FD-Score:4.01|P-value:3.02E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OSH6(YKR003W)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PAT1(YCR077C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PCL8(YPL219W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PHB2(YGR231C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PPS1(YBR276C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD18(YCR066W)|FD-Score:11.9|P-value:6.15E-33||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD5(YLR032W)|FD-Score:7.97|P-value:7.83E-16||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:8.52|P-value:7.93E-18||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RBK1(YCR036W)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative ribokinase Gene:RGL1(YPL066W)|FD-Score:5.02|P-value:2.54E-7||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RHB1(YCR027C)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RML2(YEL050C)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPH1(YER169W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RUP1(YOR138C)|FD-Score:-3.82|P-value:6.63E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:RXT3(YDL076C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SGT2(YOR007C)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:STM1(YLR150W)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TRM11(YOL124C)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TSC3(YBR058C-A)|FD-Score:19.5|P-value:5.67E-85||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBC13(YDR092W)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:URM1(YIL008W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:URN1(YPR152C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VPH2(YKL119C)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS21(YOR089C)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS38(YLR360W)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated Gene:VTC2(YFL004W)|FD-Score:4.62|P-value:1.92E-6||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YEA6(YEL006W)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER119C-A(YER119C-A_d)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGR161W-C(YGR161W-C_p)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YGR259C(YGR259C_d)|FD-Score:3.8|P-value:7.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YJL055W(YJL055W_p)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YJL119C(YJL119C_d)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR037W(YJR037W_d)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces Gene:YKR073C(YKR073C_d)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR040C(YLR040C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YNL162W-A(YNL162W-A_p)|FD-Score:-3.11|P-value:9.22E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YNL226W(YNL226W_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL024W(YOL024W_p)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPR063C(YPR063C_p)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:ER-localized protein of unknown function Gene:YPT35(YHR105W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT53(YNL093W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ZTA1(YBR046C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:NADPH-dependent quinone reductase, GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR394W6.562.67E-111.06RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YNL126W5.501.87E-80.15SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YOR259C5.354.40E-80.80RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YJL086C_d4.552.74E-60.02YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YKL145W4.523.07E-60.13RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDL195W4.395.54E-60.44SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YHR062C3.953.90E-50.04RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YFL005W3.914.56E-50.01SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YOR194C3.904.80E-50.03TOA1TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
YER013W3.875.52E-50.09PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YDL147W3.778.10E-50.61RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YDR437W3.167.77E-40.07GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YLR340W3.099.96E-40.06RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YMR298W3.030.001210.02LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YDL097C3.010.001290.13RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A19.505.67E-85TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YCR066W11.906.15E-33RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YDR076W8.527.93E-18RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YLR032W7.977.83E-16RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YGL180W6.076.22E-10ATG1Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p
YGL087C5.344.58E-8MMS2Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
YDR377W5.111.64E-7ATP17Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
YDR092W5.101.66E-7UBC13Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
YFR036W5.042.38E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YPL066W5.022.54E-7RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YER107C4.904.91E-7GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YOR089C4.711.26E-6VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YFL004W4.621.92E-6VTC2Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
YIR023W4.621.92E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YOL024W_p4.523.16E-6YOL024W_pPutative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_550
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4185.03E-248SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.128205TSC3-RPN4
0.3707.13E-191SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0263158TSC3-RPN4
0.3635.56E-183SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.104651TSC3-RPN4
0.3572.16E-177SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.111111TSC3-RPN4
0.3218.87E-142SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.152778TSC3-RPN4
0.3145.64E-135SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.194444TSC3-RPN4
0.3062.59E-128SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0985916TSC3-RPN4
0.3042.21E-126SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0823529TSC3-RPN4
0.3021.62E-124SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0533333TSC3-RPN4
0.3003.27E-123SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0810811TSC3-RPN4
0.2981.80E-121SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.134328TSC3-RPN4
0.2942.15E-118SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.125TSC3-RPN4
0.2885.16E-113SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0684932TSC3-RPN4
0.2886.68E-113SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.126437TSC3-RPN4
0.2856.69E-111SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.134146

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3281914061349.47 μM0.34782616013766Chembridge (Drug-like library)361.436923.12703RPP1 & pyrimidine depletion
SGTC_21685727979200 μM0.310345695210Chembridge (Fragment library)206.26422.23603
SGTC_7531322-0200289 μM0.3088245403907ChemDiv (Drug-like library)275.216961.85526
SGTC_10493226-013466 μM0.3015875702385ChemDiv (Drug-like library)239.226163.00304
SGTC_2131246-0773967 nM0.301376251622ChemDiv (Drug-like library)323.325963.87806
SGTC_13231348-135111.2 μM0.301376989654ChemDiv (Drug-like library)336.304882.617
SGTC_1000086-012849.59 μM0.28571493297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress
SGTC_5931203-021861.7 μM0.2857143666956ChemDiv (Drug-like library)244.33213.65302
SGTC_1678st01926580.2 μM0.2786895367020TimTec (Natural product derivative library)249.264043.6080360S ribosome export
SGTC_13853091-483414.1 μM0.2769234072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion