k242-0005

N-(2-chlorophenyl)-2-(1,4-diphenylimidazol-2-yl)sulfanylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_552
Screen concentration 476.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4088318
SMILES C1=CC=C(C=C1)C2=CN(C(=N2)SCC(=O)NC3=CC=CC=C3Cl)C4=CC=CC=C4
Standardized SMILES Clc1ccccc1NC(=O)CSc2nc(cn2c3ccccc3)c4ccccc4
Molecular weight 419.9265
ALogP 5.83
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.61
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4088318
Download HIP data (tab-delimited text)  (excel)
Gene:CDC25(YLR310C)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.03||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:ESA1(YOR244W)|FD-Score:-4.09|P-value:2.19E-5|Clearance:0||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:PDS5(YMR076C)|FD-Score:3.74|P-value:9.21E-5|Clearance:0.52||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PGA2(YNL149C)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.52||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:POP8(YBL018C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RFA2(YNL312W)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.52||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:TIM10(YHR005C-A)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.04||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TSC11(YER093C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.01||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:CDC25(YLR310C)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.03||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:ESA1(YOR244W)|FD-Score:-4.09|P-value:2.19E-5|Clearance:0||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:PDS5(YMR076C)|FD-Score:3.74|P-value:9.21E-5|Clearance:0.52||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PGA2(YNL149C)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.52||SGD DESC:Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect Gene:POP8(YBL018C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RFA2(YNL312W)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.52||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:TIM10(YHR005C-A)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.04||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TSC11(YER093C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.01||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4088318
Download HOP data (tab-delimited text)  (excel)
Gene:ACA1(YER045C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM20(YIL158W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ALY2(YJL084C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:APA1(YCL050C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:APQ13(YJL075C_d)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ASC1(YMR116C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BOI2(YER114C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BSD2(YBR290W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:BUD23(YCR047C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CAM1(YPL048W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CLN3(YAL040C)|FD-Score:4.33|P-value:7.45E-6||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COX10(YPL172C)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CUL3(YGR003W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DPB3(YBR278W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:GAD1(YMR250W)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GAL2(YLR081W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:IDP1(YDL066W)|FD-Score:-3.78|P-value:7.89E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IMD3(YLR432W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:KAR9(YPL269W)|FD-Score:-3.41|P-value:3.31E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LOC1(YFR001W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAL13(YGR288W)|FD-Score:5.16|P-value:1.27E-7||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MNL2(YLR057W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MSC2(YDR205W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSN4(YKL062W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MSR1(YHR091C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MXR1(YER042W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NHP10(YDL002C)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NNF2(YGR089W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PCL8(YPL219W)|FD-Score:5.79|P-value:3.52E-9||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEX11(YOL147C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PEX28(YHR150W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PRS5(YOL061W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PUF6(YDR496C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RCN2(YOR220W_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:REC102(YLR329W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RPI1(YIL119C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL29(YFR032C-A)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL2B(YIL018W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RRD2(YPL152W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:-5.06|P-value:2.06E-7||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCS7(YMR272C)|FD-Score:4.77|P-value:9.44E-7||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SHH4(YLR164W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SLX1(YBR228W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SNA2(YDR525W-A)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOL2(YCR073W-A)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SUM1(YDR310C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:THP2(YHR167W)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:URA2(YJL130C)|FD-Score:-6.02|P-value:8.59E-10||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR184W(YBR184W_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YER137C(YER137C_p)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Putative protein of unknown function Gene:YER158C(YER158C_p)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGL159W(YGL159W_p)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YLR434C(YLR434C_d)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YLR446W(YLR446W_p)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YMR181C(YMR181C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YNL295W(YNL295W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function Gene:YNL303W(YNL303W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL067C(YPL067C_p)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:ACA1(YER045C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ACE2(YLR131C)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM20(YIL158W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ALY2(YJL084C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:APA1(YCL050C)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:APQ13(YJL075C_d)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ASC1(YMR116C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BOI2(YER114C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BSD2(YBR290W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:BUD23(YCR047C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CAM1(YPL048W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CLN3(YAL040C)|FD-Score:4.33|P-value:7.45E-6||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COX10(YPL172C)|FD-Score:4.64|P-value:1.76E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CUL3(YGR003W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DPB3(YBR278W)|FD-Score:5|P-value:2.81E-7||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:GAD1(YMR250W)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress Gene:GAL2(YLR081W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:IDP1(YDL066W)|FD-Score:-3.78|P-value:7.89E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IMD3(YLR432W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:KAR9(YPL269W)|FD-Score:-3.41|P-value:3.31E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LOC1(YFR001W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MAL13(YGR288W)|FD-Score:5.16|P-value:1.27E-7||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MNL2(YLR057W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MSC2(YDR205W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids Gene:MSN4(YKL062W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:MSR1(YHR091C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MXR1(YER042W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NHP10(YDL002C)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NNF2(YGR089W)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PCL8(YPL219W)|FD-Score:5.79|P-value:3.52E-9||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEX11(YOL147C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PEX28(YHR150W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PRS5(YOL061W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:PUF6(YDR496C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RCN2(YOR220W_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:REC102(YLR329W)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RPI1(YIL119C)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL29(YFR032C-A)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL2B(YIL018W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RRD2(YPL152W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:-5.06|P-value:2.06E-7||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCS7(YMR272C)|FD-Score:4.77|P-value:9.44E-7||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SHH4(YLR164W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SLX1(YBR228W)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SNA2(YDR525W-A)|FD-Score:-3.97|P-value:3.61E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOL2(YCR073W-A)|FD-Score:3.73|P-value:9.65E-5||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SUM1(YDR310C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:THP2(YHR167W)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:URA2(YJL130C)|FD-Score:-6.02|P-value:8.59E-10||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:YAL042C-A(YAL042C-A_d)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YBR184W(YBR184W_p)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YER137C(YER137C_p)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Putative protein of unknown function Gene:YER158C(YER158C_p)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGL159W(YGL159W_p)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YLR434C(YLR434C_d)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YLR446W(YLR446W_p)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YMR181C(YMR181C_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YNL295W(YNL295W_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function Gene:YNL303W(YNL303W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL067C(YPL067C_p)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL149C4.261.01E-50.52PGA2Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
YNL312W4.141.72E-50.52RFA2Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YMR076C3.749.21E-50.52PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YLR310C3.226.41E-40.03CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YHR005C-A3.197.14E-40.04TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YER093C3.158.28E-40.01TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YBL018C3.148.53E-40.08POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YCL054W3.060.001100.03SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YGR265W_d3.030.001220.07YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YKL144C2.960.001540.01RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YGL120C2.950.001610.07PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YGR280C2.880.001990.04PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YAL025C2.840.002220.03MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
YKR063C2.810.002450.02LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YGR278W2.800.002570.06CWC22Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL219W5.793.52E-9PCL8Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation
YDL002C5.571.26E-8NHP10Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
YLR131C5.413.22E-8ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YGR288W5.161.27E-7MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YBR278W5.002.81E-7DPB3Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
YMR272C4.779.44E-7SCS7Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
YPL172C4.641.76E-6COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YLR434C_d4.621.93E-6YLR434C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W
YAL040C4.337.45E-6CLN3G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
YER137C_p4.327.96E-6YER137C_pPutative protein of unknown function
YCR047C4.251.05E-5BUD23Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
YGR250C4.251.08E-5YGR250CPutative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
YPL152W4.181.43E-5RRD2Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
YER158C_p4.052.61E-5YER158C_pProtein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
YKL062W3.953.89E-5MSN4Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_552
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1071.31E-16SGTC_1603453-0671 289.7 μMChemDiv (Drug-like library)53222680.0736842
0.0758.77E-9SGTC_1802st050112 57.9 μMTimTec (Natural product derivative library)46625630.0465116
0.0731.85E-8SGTC_315k915-0449 187.0 μMChemDiv (Drug-like library)209702150.0860215
0.0681.51E-7SGTC_9721319-0219 195.0 μMChemDiv (Drug-like library)239037020.105263
0.0587.38E-6SGTC_14464239-0190 62.0 μMChemDiv (Drug-like library)13400850.141176
0.0543.60E-5SGTC_368fentichlor 4.6 μMChemDiv (Drug-like library)73290.1
0.0535.32E-5SGTC_7521896-0090 130.0 μMChemDiv (Drug-like library)7725670.0779221TSC3-RPN4
0.0518.98E-5SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.0757576endomembrane recycling
0.0501.14E-4SGTC_23945221648 200.0 μMMiscellaneous7885860.117647
0.0473.06E-4SGTC_33169136639 10.5 μMChembridge (Drug-like library)49036650.144578
0.0473.15E-4SGTC_1695st026914 34.5 μMTimTec (Natural product derivative library)32891910.0786517
0.0473.31E-4SGTC_29035923464 63.9 μMChembridge (Drug-like library)28765760.1
0.0454.98E-4SGTC_24355706476 10.6 μMMiscellaneous57253750.102564
0.0455.76E-4SGTC_2629bisabolol 100.0 μMMicrosource (Natural product library)60976210.0512821
0.0447.57E-4SGTC_24775967686 25.2 μMMiscellaneous7761180.140845

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1300136-0219337.66 μM0.362069234244ChemDiv (Drug-like library)296.148643.77812
SGTC_2895905444758.44 μM0.3606562697450Chembridge (Drug-like library)343.850483.16113
SGTC_12971068-011114.1 μM0.355932767057ChemDiv (Drug-like library)312.214244.33712
SGTC_74000s-0487160.28 μM0.35677548ChemDiv (Drug-like library)277.702982.87223
SGTC_2710st07856253.72 μM0.33783817250908TimTec (Natural product derivative library)357.430062.40724
SGTC_2994905352071.43 μM0.3333334647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_2846900864219.48 μM0.3278692992898Chembridge (Drug-like library)338.219583.51423
SGTC_3233913234049.47 μM0.31884117682950Chembridge (Drug-like library)329.803983.08314
SGTC_1704197-0004226 μM0.318182218692ChemDiv (Drug-like library)317.793283.72923
SGTC_2978901178442.97 μM0.317462994934Chembridge (Drug-like library)309.38223.67423