4296-0685

6-butylsulfanyl-2-oxo-4-thiophen-2-yl-3,4-dihydro-1H-pyridine-5-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_555
Screen concentration 37.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2908540
SMILES CCCCSC1=C(C(CC(=O)N1)C2=CC=CS2)C#N
Standardized SMILES CCCCSC1=C(C#N)C(CC(=O)N1)c2cccs2
Molecular weight 292.4196
ALogP 3.12
H-bond donor count 1
H-bond acceptor count 4
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.56
% growth inhibition (Hom. pool) 10.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2908540
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:4.3|P-value:8.69E-6|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC19(YAL038W)|FD-Score:3.72|P-value:9.78E-5|Clearance:0.49||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC25(YLR310C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.23||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:ERG7(YHR072W)|FD-Score:16.1|P-value:2.13E-58|Clearance:11.62||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:NCP1(YHR042W)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NIC96(YFR002W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.05||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:SWI1(YPL016W)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.1||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF12(YDR145W)|FD-Score:4.45|P-value:4.28E-6|Clearance:0.15||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YAH1(YPL252C)|FD-Score:4.03|P-value:2.79E-5|Clearance:0.31||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YGR114C(YGR114C_d)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YNL114C(YNL114C_d)|FD-Score:-3.23|P-value:6.25E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:ARP7(YPR034W)|FD-Score:4.3|P-value:8.69E-6|Clearance:0.04||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC19(YAL038W)|FD-Score:3.72|P-value:9.78E-5|Clearance:0.49||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC25(YLR310C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.23||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:ERG7(YHR072W)|FD-Score:16.1|P-value:2.13E-58|Clearance:11.62||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:NCP1(YHR042W)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NIC96(YFR002W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.05||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:SWI1(YPL016W)|FD-Score:3.23|P-value:6.10E-4|Clearance:0.1||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF12(YDR145W)|FD-Score:4.45|P-value:4.28E-6|Clearance:0.15||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YAH1(YPL252C)|FD-Score:4.03|P-value:2.79E-5|Clearance:0.31||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YGR114C(YGR114C_d)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YNL114C(YNL114C_d)|FD-Score:-3.23|P-value:6.25E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2908540
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:AGP3(YFL055W)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:BMH2(YDR099W)|FD-Score:4.32|P-value:7.80E-6||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD6(YLR319C)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CDC73(YLR418C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.8|P-value:7.20E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRH1(YGR189C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:DFG16(YOR030W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ESL2(YKR096W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:FMP32(YFL046W_p)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HIS2(YFR025C)|FD-Score:-4.02|P-value:2.92E-5||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HXK2(YGL253W)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IGD1(YFR017C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:MGA1(YGR249W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:OPI9(YLR338W_d)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PEX12(YMR026C)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:POT1(YIL160C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPS1(YBR276C)|FD-Score:-3.84|P-value:6.17E-5||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RIC1(YLR039C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM13(YMR154C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:SDC1(YDR469W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SLC1(YDL052C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SOL1(YNR034W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPS19(YNL202W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SRC1(YML034W)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SUM1(YDR310C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TRM82(YDR165W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UTR1(YJR049C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:YBL070C(YBL070C_d)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YGL118C(YGL118C_d)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL152C(YGL152C_d)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W Gene:YGL177W(YGL177W_d)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR045C(YGR045C_d)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIL032C(YIL032C_d)|FD-Score:-5.12|P-value:1.55E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL136W(YKL136W_d)|FD-Score:6.2|P-value:2.89E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR296W(YLR296W_d)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL324W(YNL324W_d)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR170C(YPR170C_d)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ACF4(YJR083C)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:AGP3(YFL055W)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:BMH2(YDR099W)|FD-Score:4.32|P-value:7.80E-6||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BTS1(YPL069C)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD6(YLR319C)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CDC73(YLR418C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.8|P-value:7.20E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRH1(YGR189C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:DFG16(YOR030W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ESL2(YKR096W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:FMP32(YFL046W_p)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HIS2(YFR025C)|FD-Score:-4.02|P-value:2.92E-5||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HXK2(YGL253W)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IGD1(YFR017C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:MGA1(YGR249W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants Gene:OPI9(YLR338W_d)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PEX12(YMR026C)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:POT1(YIL160C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPS1(YBR276C)|FD-Score:-3.84|P-value:6.17E-5||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RIC1(YLR039C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM13(YMR154C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:SDC1(YDR469W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SLC1(YDL052C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SOL1(YNR034W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPS19(YNL202W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SRC1(YML034W)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SUM1(YDR310C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TRM82(YDR165W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:UTR1(YJR049C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:YBL070C(YBL070C_d)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YGL118C(YGL118C_d)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL152C(YGL152C_d)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W Gene:YGL177W(YGL177W_d)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR045C(YGR045C_d)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR122W(YGR122W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIL032C(YIL032C_d)|FD-Score:-5.12|P-value:1.55E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL136W(YKL136W_d)|FD-Score:6.2|P-value:2.89E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR296W(YLR296W_d)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL324W(YNL324W_d)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR170C(YPR170C_d)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR072W16.102.13E-5811.60ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YDR145W4.454.28E-60.15TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YPR034W4.308.69E-60.04ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR310C4.261.04E-50.23CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YPL252C4.032.79E-50.30YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YAL038W3.729.78E-50.49CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YPL016W3.236.10E-40.10SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YFR002W3.138.62E-40.05NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YGR245C3.080.001020.05SDA1Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
YGL075C3.040.001190.04MPS2Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p
YBL084C3.000.001360.11CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YOR353C2.890.001920.01SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YBL092W2.880.002010.01RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YFL008W2.870.002060.04SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YGR099W2.830.002310.01TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL136W_d6.202.89E-10YKL136W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
YPL069C5.305.65E-8BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YML034W5.191.03E-7SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YGL177W_d4.444.44E-6YGL177W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR099W4.327.80E-6BMH214-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YNL324W_d4.092.17E-5YNL324W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YLR285C-A_p3.993.36E-5YLR285C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YJR049C3.963.73E-5UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YLR319C3.855.97E-5BUD6Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
YPR170C_d3.826.73E-5YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YLR296W_d3.817.03E-5YLR296W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR165W3.768.52E-5TRM82Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
YMR026C3.739.61E-5PEX12C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
YFL055W3.729.87E-5AGP3Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition
YGL045W3.591.63E-4RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family

GO enrichment analysis for SGTC_555
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3434.49E-162SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0641026ergosterol biosynthesis
0.2853.58E-111SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.132353azole & statin
0.2733.02E-101SGTC_12370330-0133 46.1 μMChemDiv (Drug-like library)X12370.0810811ergosterol biosynthesis
0.2292.96E-71SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.123288azole & statin
0.2242.56E-68SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.144928ergosterol biosynthesis
0.2181.87E-64SGTC_1547harmine 94.2 μMMiscellaneous52809530.0526316ergosterol biosynthesis
0.2143.82E-62SGTC_1474466-0538 29.2 μMChemDiv (Drug-like library)67426870.0555556ergosterol biosynthesis
0.2132.33E-61SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.0779221ergosterol biosynthesis
0.2124.06E-61SGTC_29749094440 9.7 μMChembridge (Drug-like library)252361340.0952381ergosterol biosynthesis
0.1927.37E-50SGTC_269exalamide 29.0 μMMiscellaneous33160.161765ergosterol biosynthesis
0.1911.66E-49SGTC_610978-8327 98.6 μMChemDiv (Drug-like library)61500590.0641026ergosterol biosynthesis
0.1865.72E-47SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.1ergosterol biosynthesis
0.1791.29E-43SGTC_3271273-0057 4.0 μMChemDiv (Drug-like library)31081380.112676azole & statin
0.1661.05E-37SGTC_3630455-0021 3.1 μMChemDiv (Drug-like library)45974660.0632911azole & statin
0.1631.62E-36SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.128571ergosterol biosynthesis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2071523095139.92 μM0.3275861799272Chembridge (Fragment library)260.338021.20626TSC3-RPN4
SGTC_20895281248129.67 μM0.3188412737878Chembridge (Fragment library)272.322281.59515superoxide
SGTC_20705230947200 μM0.3088242835538Chembridge (Fragment library)260.311581.23215
SGTC_21465545642200 μM0.274194825326Chembridge (Fragment library)202.232441.21713
SGTC_21866124449200 μM0.2463773834182Chembridge (Fragment library)245.300241.3815RSC complex & mRNA processing
SGTC_711k292-0785179 μM0.2388064146315ChemDiv (Drug-like library)240.345083.42413DNA intercalators
SGTC_1614065-0411162.15 μM0.2352941906281ChemDiv (Drug-like library)263.381724.37604
SGTC_11463579-10237 μM0.219178X1146ChemDiv (Drug-like library)381.507835.48843
SGTC_10064099-6547255 μM0.2173913146833ChemDiv (Drug-like library)287.3039063.94313
SGTC_20905301565104.45 μM0.2153852056344Chembridge (Fragment library)274.36461.69226TSC3-RPN4