4058-0063

5-[(3-bromo-4-phenylmethoxyphenyl)methylidene]-2-sulfanylidene-1,3-diazinane-4,6-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_556
Screen concentration 51.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 6615692
SMILES C1=CC=C(C=C1)COC2=C(C=C(C=C2)C=C3C(=O)NC(=S)NC3=O)Br
Standardized SMILES Brc1cc(C=C2C(=O)NC(=S)NC2=O)ccc1OCc3ccccc3
Molecular weight 417.2764
ALogP 4.08
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.58
% growth inhibition (Hom. pool) 8.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6615692
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:4.45|P-value:4.30E-6|Clearance:0.35||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:FOL2(YGR267C)|FD-Score:3.94|P-value:4.01E-5|Clearance:0.35||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GDI1(YER136W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.35||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPM1(YKL152C)|FD-Score:-4.55|P-value:2.72E-6|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:IRA1(YBR140C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.35||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MED6(YHR058C)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.35||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NOC3(YLR002C)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.12||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:PFS2(YNL317W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.35||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PRP3(YDR473C)|FD-Score:3.77|P-value:8.08E-5|Clearance:0.35||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPL15A(YLR029C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.35||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPT3(YDR394W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RTT105(YER104W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.35||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC31(YDL195W)|FD-Score:-3.16|P-value:7.97E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:STN1(YDR082W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.07||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:AAR2(YBL074C)|FD-Score:4.45|P-value:4.30E-6|Clearance:0.35||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:FOL2(YGR267C)|FD-Score:3.94|P-value:4.01E-5|Clearance:0.35||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GDI1(YER136W)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.35||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPM1(YKL152C)|FD-Score:-4.55|P-value:2.72E-6|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:IRA1(YBR140C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.35||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MED6(YHR058C)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.35||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:NOC3(YLR002C)|FD-Score:3.27|P-value:5.32E-4|Clearance:0.12||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:PFS2(YNL317W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.35||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PRP3(YDR473C)|FD-Score:3.77|P-value:8.08E-5|Clearance:0.35||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPL15A(YLR029C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.35||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPT3(YDR394W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RTT105(YER104W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.35||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC31(YDL195W)|FD-Score:-3.16|P-value:7.97E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:STN1(YDR082W)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.07||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6615692
Download HOP data (tab-delimited text)  (excel)
Gene:APN1(YKL114C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARO1(YDR127W)|FD-Score:6.59|P-value:2.27E-11||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:5.36|P-value:4.16E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BNA2(YJR078W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:CAP2(YIL034C)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CAR2(YLR438W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CHS6(YJL099W)|FD-Score:4.29|P-value:8.89E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COQ9(YLR201C)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:CTP1(YBR291C)|FD-Score:-4.38|P-value:5.82E-6||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CTR9(YOL145C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:8.78|P-value:8.19E-19||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUF1(YOL087C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM27(YJR106W)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EXO5(YBR163W)|FD-Score:3.75|P-value:8.94E-5||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FUR4(YBR021W)|FD-Score:6.46|P-value:5.39E-11||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FUS2(YMR232W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GAP1(YKR039W)|FD-Score:-4.33|P-value:7.38E-6||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCN2(YDR283C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:5.5|P-value:1.91E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP3(YOR205C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GPR1(YDL035C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GYP1(YOR070C)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HSP26(YBR072W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:KXD1(YGL079W_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LYS12(YIL094C)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MDM1(YML104C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MSC1(YML128C)|FD-Score:-4.83|P-value:6.88E-7||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NPL4(YBR170C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PDX3(YBR035C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PRO1(YDR300C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRR1(YKL116C)|FD-Score:-3.96|P-value:3.75E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PTK2(YJR059W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RKR1(YMR247C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPL31A(YDL075W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:7.13|P-value:4.90E-13||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SDS23(YGL056C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SHR5(YOL110W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SMA2(YML066C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNX4(YJL036W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPO11(YHL022C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SVL3(YPL032C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWR1(YDR334W)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT1(YBR069C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THR4(YCR053W)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRP1(YDR007W)|FD-Score:6.06|P-value:6.76E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.66|P-value:2.28E-18||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.57|P-value:2.53E-11||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBA3(YPR066W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URA2(YJL130C)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:YBR074W(YBR074W_p)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Putative metalloprotease Gene:YBR134W(YBR134W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCL049C(YCL049C_p)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDL172C(YDL172C_d)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:5.26|P-value:7.16E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function Gene:YGL159W(YGL159W_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJR079W(YJR079W_p)|FD-Score:6.85|P-value:3.58E-12||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKL031W(YKL031W_d)|FD-Score:6.6|P-value:2.02E-11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLR241W(YLR241W_p)|FD-Score:-3.8|P-value:7.37E-5||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR358C(YLR358C_p)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YNR021W(YNR021W_p)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YOR072W(YOR072W_d)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPC1(YBR183W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:APN1(YKL114C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARO1(YDR127W)|FD-Score:6.59|P-value:2.27E-11||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:5.36|P-value:4.16E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BNA2(YJR078W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase, required for de novo biosynthesis of NAD from tryptophan via kynurenine; interacts genetically with telomere capping gene CDC13; regulated by Hst1p and Aftp Gene:CAP2(YIL034C)|FD-Score:4.38|P-value:5.83E-6||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CAR2(YLR438W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CHS6(YJL099W)|FD-Score:4.29|P-value:8.89E-6||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COQ9(YLR201C)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:CTP1(YBR291C)|FD-Score:-4.38|P-value:5.82E-6||SGD DESC:Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family Gene:CTR9(YOL145C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:8.78|P-value:8.19E-19||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUF1(YOL087C)|FD-Score:3.96|P-value:3.70E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM27(YJR106W)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EXO5(YBR163W)|FD-Score:3.75|P-value:8.94E-5||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FUR4(YBR021W)|FD-Score:6.46|P-value:5.39E-11||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FUS2(YMR232W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GAP1(YKR039W)|FD-Score:-4.33|P-value:7.38E-6||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCN2(YDR283C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:5.5|P-value:1.91E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP3(YOR205C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GPR1(YDL035C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GYP1(YOR070C)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HSP26(YBR072W)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:KXD1(YGL079W_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome Gene:LYS12(YIL094C)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MDM1(YML104C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MSC1(YML128C)|FD-Score:-4.83|P-value:6.88E-7||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NPL4(YBR170C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PDX3(YBR035C)|FD-Score:5.19|P-value:1.08E-7||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PRO1(YDR300C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:PRR1(YKL116C)|FD-Score:-3.96|P-value:3.75E-5||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PTK2(YJR059W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:RKR1(YMR247C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPL31A(YDL075W)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:7.13|P-value:4.90E-13||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SDS23(YGL056C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SHR5(YOL110W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SMA2(YML066C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNX4(YJL036W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPO11(YHL022C)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SVL3(YPL032C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWR1(YDR334W)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT1(YBR069C)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THR4(YCR053W)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRP1(YDR007W)|FD-Score:6.06|P-value:6.76E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.11|P-value:1.96E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.66|P-value:2.28E-18||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.57|P-value:2.53E-11||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBA3(YPR066W)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URA2(YJL130C)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:YBR074W(YBR074W_p)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Putative metalloprotease Gene:YBR134W(YBR134W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCL049C(YCL049C_p)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDL172C(YDL172C_d)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:5.26|P-value:7.16E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative protein of unknown function Gene:YGL159W(YGL159W_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJR079W(YJR079W_p)|FD-Score:6.85|P-value:3.58E-12||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YKL031W(YKL031W_d)|FD-Score:6.6|P-value:2.02E-11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YLR241W(YLR241W_p)|FD-Score:-3.8|P-value:7.37E-5||SGD DESC:Putative protein of unknown function, may be involved in detoxification Gene:YLR358C(YLR358C_p)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YNR021W(YNR021W_p)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YOR072W(YOR072W_d)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPC1(YBR183W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL074C4.454.30E-60.35AAR2Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
YER104W4.221.23E-50.35RTT105Protein with a role in regulation of Ty1 transposition
YBR140C4.191.38E-50.35IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YHR058C4.171.50E-50.35MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YGR267C3.944.01E-50.35FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YDR473C3.778.08E-50.35PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YLR029C3.721.00E-40.35RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YNL317W3.641.37E-40.35PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YER136W3.621.47E-40.35GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YLR002C3.275.32E-40.12NOC3Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
YDR082W3.167.98E-40.07STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YER003C3.080.001030.11PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YFL039C2.970.001480.01ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YOL040C2.960.001520.01RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YGR156W2.950.001570.13PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W8.788.19E-19DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKL211C8.662.28E-18TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL244W7.134.90E-13RTF1Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YJR079W_p6.853.58E-12YJR079W_pPutative protein of unknown function; mutation results in impaired mitochondrial respiration
YKL031W_d6.602.02E-11YKL031W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
YDR127W6.592.27E-11ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR354W6.572.53E-11TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YBR021W6.465.39E-11FUR4Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
YDR007W6.066.76E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YEL009C5.501.91E-8GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR035W5.364.16E-8ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YDR008C_d5.267.16E-8YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YBR035C5.191.08E-7PDX3Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
YLR358C_p4.983.21E-7YLR358C_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
YDR334W4.963.60E-7SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A

GO enrichment analysis for SGTC_556
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3509.39E-170SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0519481
0.3416.20E-161SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0322581
0.3417.68E-161SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0309278
0.3273.30E-147SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0540541
0.3262.08E-146SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0675676
0.3205.98E-141SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0519481
0.3172.85E-138SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0149254
0.3179.62E-138SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0857143
0.3171.17E-137SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.104651
0.3155.53E-136SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0705882
0.3148.96E-135SGTC_18825615643 20.0 μMMiscellaneous22530750.1625TRP & mitochondrial translation
0.3111.59E-132SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.145161
0.3094.53E-131SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.084507
0.3097.25E-131SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.030303
0.3091.09E-130SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0813954

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5883970-114452.9 μM0.4827591888707ChemDiv (Drug-like library)425.2609434.86425
SGTC_23276189656200 μM0.4339625349088Chembridge (Fragment library)282.702882.18634
SGTC_1631benzylvanillin81.9 μM0.40740775506TimTec (Natural product derivative library)242.269863.1403Golgi
SGTC_6033794-002345.9 μM0.4035093802260ChemDiv (Drug-like library)356.759823.09624
SGTC_1708st03323431.83 μM0.403509674664TimTec (Natural product derivative library)321.165923.88803
SGTC_1716st03314430.62 μM0.396552674617TimTec (Natural product derivative library)335.19254.23703
SGTC_2065522159634.26 μM0.358491788581Chembridge (Fragment library)238.286141.71724
SGTC_23067482397179.52 μM0.333333891448Chembridge (Fragment library)282.702882.18634RPP1 & pyrimidine depletion
SGTC_1714st03323613.63 μM0.3076922916566TimTec (Natural product derivative library)355.610984.55203
SGTC_505gw-507463.9 μM0.3064525924208ICCB bioactive library520.941994.56422