1550-0661

(E)-3-(4-chlorophenyl)-N-[2-(2,5-dichlorophenyl)-1,3-benzoxazol-5-yl]prop-2-en-1-imine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_559
Screen concentration 158.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5830875
SMILES C1=CC(=CC=C1C=CC=NC2=CC3=C(C=C2)OC(=N3)C4=C(C=CC(=C4)Cl)Cl)Cl
Standardized SMILES Clc1ccc(C=CC=Nc2ccc3oc(nc3c2)c4cc(Cl)ccc4Cl)cc1
Molecular weight 427.7104
ALogP 7.23
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.07
% growth inhibition (Hom. pool) 6.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5830875
Download HIP data (tab-delimited text)  (excel)
Gene:FOL2(YGR267C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.01||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:MCM7(YBR202W)|FD-Score:3.84|P-value:6.04E-5|Clearance:0.34||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MTR3(YGR158C)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:PRE1(YER012W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.34||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRI2(YKL045W)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.34||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RMP1(YLR145W)|FD-Score:-3.73|P-value:9.58E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPB10(YOR210W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RVB1(YDR190C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SMP3(YOR149C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.34||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SUA7(YPR086W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II Gene:FOL2(YGR267C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.01||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:MCM7(YBR202W)|FD-Score:3.84|P-value:6.04E-5|Clearance:0.34||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MTR3(YGR158C)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:PRE1(YER012W)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.34||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRI2(YKL045W)|FD-Score:3.55|P-value:1.92E-4|Clearance:0.34||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RMP1(YLR145W)|FD-Score:-3.73|P-value:9.58E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPB10(YOR210W)|FD-Score:3.13|P-value:8.62E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RVB1(YDR190C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SMP3(YOR149C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.34||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SUA7(YPR086W)|FD-Score:-3.52|P-value:2.18E-4|Clearance:0||SGD DESC:Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5830875
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ1(YNR033W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:AGP3(YFL055W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AIM14(YGL160W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ARD1(YHR013C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ASN1(YPR145W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:COQ9(YLR201C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:COX12(YLR038C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CTF4(YPR135W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:4.88|P-value:5.36E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFM1(YDR411C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DJP1(YIR004W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:FUI1(YBL042C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAL2(YLR081W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GCN4(YEL009C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:ODC2(YOR222W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PIN3(YPR154W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PRM5(YIL117C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:RAD18(YCR066W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RBG1(YAL036C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RPL21A(YBR191W)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SCS7(YMR272C)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEM1(YDR363W-A)|FD-Score:-3.82|P-value:6.56E-5||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SNA2(YDR525W-A)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUM1(YDR310C)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TGS1(YPL157W)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRP2(YER090W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS3(YDR495C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS72(YDR485C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YBR016W(YBR016W)|FD-Score:6.84|P-value:4.09E-12||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDR131C(YDR131C_p)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YDR215C(YDR215C_d)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YGL177W(YGL177W_d)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR259C(YGR259C_d)|FD-Score:-3.76|P-value:8.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YIL054W(YIL054W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YKL018C-A(YKL018C-A_p)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR046C(YLR046C_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR282C(YLR282C_d)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNR040W(YNR040W_p)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL057W(YOL057W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YPR099C(YPR099C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPT35(YHR105W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ABZ1(YNR033W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:AGP3(YFL055W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition Gene:AIM14(YGL160W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ARD1(YHR013C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ASN1(YPR145W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BCK1(YJL095W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:COQ9(YLR201C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:COX12(YLR038C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CTF4(YPR135W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DAL81(YIR023W)|FD-Score:4.88|P-value:5.36E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFM1(YDR411C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DJP1(YIR004W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:FUI1(YBL042C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAL2(YLR081W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GCN4(YEL009C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:ODC2(YOR222W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PIN3(YPR154W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:PRM5(YIL117C)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:RAD18(YCR066W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RBG1(YAL036C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RPL21A(YBR191W)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SCS7(YMR272C)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEM1(YDR363W-A)|FD-Score:-3.82|P-value:6.56E-5||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SNA2(YDR525W-A)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUM1(YDR310C)|FD-Score:-4.19|P-value:1.41E-5||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TGS1(YPL157W)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRP2(YER090W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS3(YDR495C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS72(YDR485C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAL044W-A(YAL044W-A_p)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YBR016W(YBR016W)|FD-Score:6.84|P-value:4.09E-12||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YDR131C(YDR131C_p)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YDR215C(YDR215C_d)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YGL177W(YGL177W_d)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR259C(YGR259C_d)|FD-Score:-3.76|P-value:8.52E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YIL054W(YIL054W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YKL018C-A(YKL018C-A_p)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Putative protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR046C(YLR046C_p)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR282C(YLR282C_d)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNR040W(YNR040W_p)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL057W(YOL057W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YPR099C(YPR099C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPT35(YHR105W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR202W3.846.04E-50.34MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YKL045W3.551.92E-40.34PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YER012W3.532.04E-40.34PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YOR149C3.482.50E-40.34SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YGR267C3.148.40E-40.01FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YOR210W3.138.62E-40.03RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YGR158C3.119.40E-40.07MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YLL035W3.040.001180.02GRC3Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
YIL019W3.020.001270.07FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YGL030W2.950.001570.02RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YGR140W2.930.001690.12CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YDR145W2.820.002440.04TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YNL308C2.780.002750.03KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YHR196W2.750.002992.91E-4UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR367W2.750.002990.04KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR016W6.844.09E-12YBR016WTail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
YIR023W4.885.36E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YMR272C4.356.80E-6SCS7Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
YGL160W4.211.30E-5AIM14NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
YEL009C4.121.93E-5GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YPR099C_d4.092.14E-5YPR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W
YNR040W_p4.042.70E-5YNR040W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR525W-A3.865.66E-5SNA2Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YAL044W-A_p3.739.76E-5YAL044W-A_pPutative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein
YPL152W3.671.19E-4RRD2Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
YLR046C_p3.671.22E-4YLR046C_pPutative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
YCR066W3.651.32E-4RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YPR154W3.621.45E-4PIN3Protein that induces appearance of [PIN+] prion when overproduced
YLR282C_d3.591.68E-4YLR282C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition
YIL054W_p3.522.16E-4YIL054W_pProtein of unknown function; expressed at both mRNA and protein levels

GO enrichment analysis for SGTC_559
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1575.86E-34SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0533333
0.1511.79E-31SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)5150859NA
0.1455.96E-29SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.0540541
0.1456.08E-29SGTC_6033794-0023 45.9 μMChemDiv (Drug-like library)38022600.152778
0.1381.31E-26SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0945946
0.1372.99E-26SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0609756
0.1352.36E-25SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.0632911
0.1313.79E-24SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0206186
0.1314.49E-24SGTC_11811222-0449 220.0 μMChemDiv (Drug-like library)6583180.0526316
0.1301.02E-23SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.0958904
0.1263.78E-22SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.0769231
0.1241.27E-21SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.148649
0.1224.76E-21SGTC_486niguldipine 82.0 μMMiscellaneous12360.037037amide catabolism
0.1226.49E-21SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.113636
0.1211.01E-20SGTC_24615755302 26.1 μMMiscellaneous27889040.0348837

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7192810-423027.3 μM0.538462721079ChemDiv (Drug-like library)279.121423.86213
SGTC_7381315-000372.4 μM0.4923086251474ChemDiv (Drug-like library)372.846886.38803
SGTC_24005203494178.7 μM0.4305566777674Miscellaneous399.398784.55216RSC complex & mRNA processing
SGTC_6941315-043843.2 μM0.4142866793154ChemDiv (Drug-like library)378.80843.90215
SGTC_3690957-03934.5 μM0.3866676997992ChemDiv (Drug-like library)413.425365.03816cell wall signaling
SGTC_580957-03939 μM0.3866676997992ChemDiv (Drug-like library)413.425365.03816cell wall
SGTC_7771315-0167517 μM0.3823534221767ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_7981287-0182476 μM0.3731341649755ChemDiv (Drug-like library)420.301825.78704
SGTC_170960-027649.81 μM0.3636366821114ChemDiv (Drug-like library)471.731044.70315
SGTC_1100850-0284111.64 μM0.3478263906265ChemDiv (Drug-like library)391.26066.11103
SGTC_6051315-0399119 μM0.34782624020461ChemDiv (Drug-like library)381.254666.69203Golgi