tetrahydrosecaminediol

[10-[2-(3-ethylpiperidin-1-yl)ethyl]-6-[3-[2-(3-ethylpiperidin-1-yl)ethyl]-1H-indol-2-yl]-6-(hydroxymethyl)-8,9-dihydro-7H-pyrido[1,2-a]indol-9-yl]methanol

A psychoactive constituent of the cannabis plant.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_563
Screen concentration 614.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 334274
SMILES CCC1CCCN(C1)CCC2=C(NC3=CC=CC=C32)C4(CCC(C5=C(C6=CC=CC=C6N54)CCN7CCCC(C7)CC)CO)CO
Standardized SMILES CCC1CCCN(CCc2c3C(CO)CCC(CO)(c4[nH]c5ccccc5c4CCN6CCCC(CC)C6)n3c7ccccc27)C1
Molecular weight 624.8982
ALogP 8.25
H-bond donor count 3
H-bond acceptor count 4
Response signature amide catabolism

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.52
% growth inhibition (Hom. pool) 6.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 334274
Download HIP data (tab-delimited text)  (excel)
Gene:DSL1(YNL258C)|FD-Score:3.26|P-value:5.64E-4|Clearance:0.11||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:MCM7(YBR202W)|FD-Score:5.09|P-value:1.77E-7|Clearance:1.2||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PRE1(YER012W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.21||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRP19(YLL036C)|FD-Score:3.14|P-value:8.33E-4|Clearance:0.1||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RIO2(YNL207W)|FD-Score:3.89|P-value:4.93E-5|Clearance:0.26||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RLP24(YLR009W)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.07||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:TID3(YIL144W)|FD-Score:-3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP18(YJL069C)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.02||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:YCR013C(YCR013C_d)|FD-Score:6.53|P-value:3.21E-11|Clearance:1.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YNL247W(YNL247W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.08||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:DSL1(YNL258C)|FD-Score:3.26|P-value:5.64E-4|Clearance:0.11||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:MCM7(YBR202W)|FD-Score:5.09|P-value:1.77E-7|Clearance:1.2||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:PRE1(YER012W)|FD-Score:3.54|P-value:1.98E-4|Clearance:0.21||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRP19(YLL036C)|FD-Score:3.14|P-value:8.33E-4|Clearance:0.1||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RIO2(YNL207W)|FD-Score:3.89|P-value:4.93E-5|Clearance:0.26||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RLP24(YLR009W)|FD-Score:3.33|P-value:4.33E-4|Clearance:0.07||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:TID3(YIL144W)|FD-Score:-3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:UTP18(YJL069C)|FD-Score:3.56|P-value:1.87E-4|Clearance:0.02||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:YCR013C(YCR013C_d)|FD-Score:6.53|P-value:3.21E-11|Clearance:1.44||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YNL247W(YNL247W)|FD-Score:3.64|P-value:1.37E-4|Clearance:0.08||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 334274
Download HOP data (tab-delimited text)  (excel)
Gene:AIM26(YKL037W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM36(YMR157C)|FD-Score:6.73|P-value:8.30E-12||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AIM41(YOR215C)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ARA1(YBR149W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARO1(YDR127W)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP10(YLR393W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BUD23(YCR047C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CHL4(YDR254W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:DAL81(YIR023W)|FD-Score:9.37|P-value:3.63E-21||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSD1(YGL196W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ERG2(YMR202W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FCY21(YER060W)|FD-Score:6.27|P-value:1.77E-10||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:HOM6(YJR139C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOR7(YMR251W-A)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:ILV1(YER086W)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:KCH1(YJR054W_p)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LAG1(YHL003C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LOC1(YFR001W)|FD-Score:4.39|P-value:5.59E-6||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MCM21(YDR318W)|FD-Score:4.82|P-value:7.04E-7||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MRPL28(YDR462W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL6(YHR147C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSC3(YLR219W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NCL1(YBL024W)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NST1(YNL091W)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PEP4(YPL154C)|FD-Score:4.32|P-value:7.77E-6||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEP7(YDR323C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET117(YER058W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PPS1(YBR276C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD9(YDR217C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RNH201(YNL072W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RRF1(YHR038W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:SAS4(YDR181C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SIL1(YOL031C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SMI1(YGR229C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SWD1(YAR003W)|FD-Score:5.38|P-value:3.81E-8||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWI3(YJL176C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TEL1(YBL088C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TRP1(YDR007W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBC8(YEL012W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBR2(YLR024C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UPF3(YGR072W)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VMA16(YHR026W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YBR138C(YBR138C_p)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YER077C(YER077C_p)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YNL228W(YNL228W_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPL067C(YPL067C_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:AIM26(YKL037W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AIM36(YMR157C)|FD-Score:6.73|P-value:8.30E-12||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:AIM41(YOR215C)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ARA1(YBR149W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARO1(YDR127W)|FD-Score:5.49|P-value:1.98E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP10(YLR393W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BUD23(YCR047C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CHL4(YDR254W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:DAL81(YIR023W)|FD-Score:9.37|P-value:3.63E-21||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DSD1(YGL196W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ERG2(YMR202W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FCY21(YER060W)|FD-Score:6.27|P-value:1.77E-10||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:HOM6(YJR139C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOR7(YMR251W-A)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:ILV1(YER086W)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:KCH1(YJR054W_p)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate Gene:LAG1(YHL003C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LOC1(YFR001W)|FD-Score:4.39|P-value:5.59E-6||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MCM21(YDR318W)|FD-Score:4.82|P-value:7.04E-7||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MRPL28(YDR462W)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL6(YHR147C)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSC3(YLR219W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress Gene:NCL1(YBL024W)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NST1(YNL091W)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PEP4(YPL154C)|FD-Score:4.32|P-value:7.77E-6||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PEP7(YDR323C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET117(YER058W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:Protein required for assembly of cytochrome c oxidase Gene:PPS1(YBR276C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:RAD9(YDR217C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RNH201(YNL072W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RRF1(YHR038W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:SAS4(YDR181C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SIL1(YOL031C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SMI1(YGR229C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SWD1(YAR003W)|FD-Score:5.38|P-value:3.81E-8||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWI3(YJL176C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TEL1(YBL088C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:TRP1(YDR007W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBC8(YEL012W)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:UBR2(YLR024C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UPF3(YGR072W)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VMA16(YHR026W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YBR138C(YBR138C_p)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:-3.96|P-value:3.81E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YER077C(YER077C_p)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YNL228W(YNL228W_d)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPL067C(YPL067C_p)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR013C_d6.533.21E-111.44YCR013C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
YBR202W5.091.77E-71.20MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YNL207W3.894.93E-50.26RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YNL247W3.641.37E-40.08YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YJL069C3.561.87E-40.02UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
YER012W3.541.98E-40.21PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YLR009W3.334.33E-40.07RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YNL258C3.265.64E-40.11DSL1Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene
YLL036C3.148.33E-40.10PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YDR288W3.050.001160.24NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YER172C2.800.002540.12BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YMR290C2.680.003710.07HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YKL099C2.610.004580.01UTP11Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YIL019W2.600.004690.01FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YKL035W2.590.004800.00UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W9.373.63E-21DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YMR157C6.738.30E-12AIM36Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
YER060W6.271.77E-10FCY21Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
YBR138C_p5.571.29E-8YBR138C_pCytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
YDR127W5.491.98E-8ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YAR003W5.383.81E-8SWD1Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
YDR318W4.827.04E-7MCM21Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
YER058W4.711.27E-6PET117Protein required for assembly of cytochrome c oxidase
YJL176C4.523.15E-6SWI3Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
YFR001W4.395.59E-6LOC1Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress
YNL091W4.366.41E-6NST1Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
YER086W4.356.82E-6ILV1Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
YPL154C4.327.77E-6PEP4Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates
YLR393W4.181.46E-5ATP10Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6
YDR254W4.161.56E-5CHL4Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15

GO enrichment analysis for SGTC_563
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2553.39E-88SGTC_486niguldipine 82.0 μMMiscellaneous12360.1amide catabolism
0.2202.22E-65SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.13253amide catabolism
0.2151.09E-62SGTC_24605552655 174.2 μMMiscellaneous54019890.355932amide catabolism
0.1781.76E-43SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.136986amide catabolism
0.1781.81E-43SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.123288amide catabolism
0.1782.76E-43SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.168831
0.1693.58E-39SGTC_9741326-0144 138.0 μMChemDiv (Drug-like library)54118560.0746269ERG2
0.1661.12E-37SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.134328
0.1632.21E-36SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.112676
0.1612.15E-35SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.084507
0.1591.51E-34SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.142857
0.1584.16E-34SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0958904
0.1554.88E-33SGTC_253dyclonine 31.2 μMMiscellaneous31800.0921053amide catabolism
0.1556.23E-33SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.173913amide catabolism
0.1541.65E-32SGTC_32289131799 49.5 μMChembridge (Drug-like library)170158180.157895amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1166k206-0078225 μM0.543865404595ChemDiv (Drug-like library)325.749083.40214
SGTC_30833-058795.58 μM0.4423085382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_9781416-051287.4 μM0.46814003ChemDiv (Drug-like library)270.283260.69424redox potentiating
SGTC_7051124-0212310 μM0.3620699554485ChemDiv (Drug-like library)293.1484.01212
SGTC_24605552655174.22 μM0.3559325401989Miscellaneous259.6912.62513amide catabolism
SGTC_24635473429200 μM0.3333335399405Miscellaneous273.717582.23113ERG2
SGTC_813k007-0158173 μM0.3166676523916ChemDiv (Drug-like library)307.776864.60713fatty acid desaturase (OLE1)
SGTC_1731st03596463 μM0.3076926739575TimTec (Natural product derivative library)317.253640.26547
SGTC_10513253-390378 μM0.3043485509239ChemDiv (Drug-like library)438.105445.44223
SGTC_21945927483104.55 μM0.303571760979Chembridge (Fragment library)201.221241.89112