3555-0129

1-benzylsulfanyl-3,3-dimethyl-2-azaspiro[4.5]deca-1,6,9-trien-8-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_564
Screen concentration 330.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 563260
SMILES CC1(CC2(C=CC(=O)C=C2)C(=N1)SCC3=CC=CC=C3)C
Standardized SMILES CC1(C)CC2(C=CC(=O)C=C2)C(=N1)SCc3ccccc3
Molecular weight 297.4146
ALogP 3.67
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.5
% growth inhibition (Hom. pool) 5.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 563260
Download HIP data (tab-delimited text)  (excel)
Gene:CDC34(YDR054C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.1||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:CFD1(YIL003W)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.16||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:MEX67(YPL169C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.18||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:ORC6(YHR118C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.13||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PRE6(YOL038W)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.17||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PZF1(YPR186C)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.07||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RNA15(YGL044C)|FD-Score:-3.46|P-value:2.69E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RSA4(YCR072C)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.05||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SKP1(YDR328C)|FD-Score:5.63|P-value:9.04E-9|Clearance:1.69||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SLD5(YDR489W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SPT16(YGL207W)|FD-Score:-4.9|P-value:4.84E-7|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:SUP45(YBR143C)|FD-Score:-3.31|P-value:4.69E-4|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:UTP21(YLR409C)|FD-Score:-3.16|P-value:7.90E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:CDC34(YDR054C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.1||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:CFD1(YIL003W)|FD-Score:3.81|P-value:6.90E-5|Clearance:0.16||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:MEX67(YPL169C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.18||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:ORC6(YHR118C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.13||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PRE6(YOL038W)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.17||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PZF1(YPR186C)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.07||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RNA15(YGL044C)|FD-Score:-3.46|P-value:2.69E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RSA4(YCR072C)|FD-Score:3.86|P-value:5.60E-5|Clearance:0.05||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SKP1(YDR328C)|FD-Score:5.63|P-value:9.04E-9|Clearance:1.69||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SLD5(YDR489W)|FD-Score:-3.27|P-value:5.37E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SPT16(YGL207W)|FD-Score:-4.9|P-value:4.84E-7|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:SUP45(YBR143C)|FD-Score:-3.31|P-value:4.69E-4|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:UTP21(YLR409C)|FD-Score:-3.16|P-value:7.90E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 563260
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ATG3(YNR007C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CAF20(YOR276W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:EAF7(YNL136W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:FRT2(YAL028W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FYV12(YOR183W_p)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:HOM6(YJR139C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:LOT6(YLR011W)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:MFB1(YDR219C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MOT2(YER068W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:PMT1(YDL095W)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPT1(YGR123C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:RPL29(YFR032C-A)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL34B(YIL052C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SAF1(YBR280C)|FD-Score:-4.24|P-value:1.09E-5||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAG1(YJR004C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SER33(YIL074C)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHO1(YER118C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SLX4(YLR135W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SRB8(YCR081W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSK2(YNR031C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:UBA3(YPR066W)|FD-Score:-3.78|P-value:8.00E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UIP5(YKR044W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:VID30(YGL227W)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS30(YPL120W)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR101C(YCR101C_p)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YEL028W(YEL028W_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL169W(YJL169W_d)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YLR040C(YLR040C_p)|FD-Score:4.99|P-value:3.01E-7||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR455W(YLR455W_p)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YOL114C(YOL114C_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOL164W-A(YOL164W-A_p)|FD-Score:3.74|P-value:9.02E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:AGC1(YPR021C)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ATG3(YNR007C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CAF20(YOR276W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:EAF7(YNL136W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:FRT2(YAL028W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FYV12(YOR183W_p)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:HOM6(YJR139C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:LOT6(YLR011W)|FD-Score:4.29|P-value:8.98E-6||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:MFB1(YDR219C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MOT2(YER068W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:PMT1(YDL095W)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPT1(YGR123C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:RPL29(YFR032C-A)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL34B(YIL052C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SAF1(YBR280C)|FD-Score:-4.24|P-value:1.09E-5||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAG1(YJR004C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SER33(YIL074C)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHO1(YER118C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SLX4(YLR135W)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SRB8(YCR081W)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSK2(YNR031C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:UBA3(YPR066W)|FD-Score:-3.78|P-value:8.00E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UIP5(YKR044W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:VID30(YGL227W)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm Gene:VPS30(YPL120W)|FD-Score:5.39|P-value:3.47E-8||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR101C(YCR101C_p)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YEL028W(YEL028W_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL012W(YIL012W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL169W(YJL169W_d)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YLR040C(YLR040C_p)|FD-Score:4.99|P-value:3.01E-7||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR455W(YLR455W_p)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YOL114C(YOL114C_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOL164W-A(YOL164W-A_p)|FD-Score:3.74|P-value:9.02E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR328C5.639.04E-91.69SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YPR186C3.944.15E-50.07PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YCR072C3.865.60E-50.05RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YIL003W3.816.90E-50.16CFD1Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
YHR118C3.651.32E-40.13ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YOL038W3.512.20E-40.17PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YPL169C3.354.09E-40.18MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YDR054C3.177.61E-40.10CDC34Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
YDL147W3.070.001070.00RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YMR290C3.070.001070.14HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YOR160W2.930.001700.04MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YGR175C2.890.001950.02ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YOR181W2.860.002110.17LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YKL052C2.690.003590.03ASK1Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
YKL088W2.650.003970.11CAB3Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL120W5.393.47E-8VPS30Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
YLR040C_p4.993.01E-7YLR040C_pProtein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
YLR135W4.464.16E-6SLX4Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
YLR011W4.298.98E-6LOT6FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
YGL227W3.807.30E-5VID30Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
YOL164W-A_p3.749.02E-5YOL164W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YIL052C3.701.08E-4RPL34BRibosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
YJR139C3.671.22E-4HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YBR056W-A_p3.561.84E-4YBR056W-A_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B
YNL213C3.492.46E-4RRG9Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YAL028W3.472.61E-4FRT2Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication
YFR032C-A3.403.32E-4RPL29Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
YGR123C3.403.37E-4PPT1Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
YCR101C_p3.393.46E-4YCR101C_pPutative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
YOL114C_p3.393.52E-4YOL114C_pPutative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene

GO enrichment analysis for SGTC_564
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1171.32E-19SGTC_492tmb-8 126.0 μMICCB bioactive library54940.0704225
0.1102.69E-17SGTC_5671080-0542 68.4 μMChemDiv (Drug-like library)59504150.0555556
0.0571.38E-5SGTC_1954234-0266 16.7 μMChemDiv (Drug-like library)23033540.109375
0.0561.88E-5SGTC_12710832-2808 169.0 μMChemDiv (Drug-like library)36637940.0952381
0.0543.88E-5SGTC_27947785382 27.3 μMChembridge (Drug-like library)22050810.0945946
0.0534.53E-5SGTC_3710195-0006 7.4 μMChemDiv (Drug-like library)39285930.125
0.0525.63E-5SGTC_2144373-3496 70.0 μMChemDiv (Drug-like library)37791660.116279
0.0525.86E-5SGTC_31959113082 49.5 μMChembridge (Drug-like library)170180380.0735294
0.0518.73E-5SGTC_1903970-0790 3.7 μMChemDiv (Drug-like library)28323520.0769231
0.0501.31E-4SGTC_9242046-0044 21.3 μMChemDiv (Drug-like library)36235890.112903
0.0501.41E-4SGTC_13321480-0653 17.5 μMChemDiv (Drug-like library)57494380.109375TSC3-RPN4
0.0473.48E-4SGTC_13782093-0007 380.0 nMChemDiv (Drug-like library)36180140.0727273
0.0464.10E-4SGTC_29197978188 46.4 μMChembridge (Drug-like library)29802910.136986
0.0464.19E-4SGTC_2233sulbentine 40.4 μMMiscellaneous676860.172414
0.0464.24E-4SGTC_690glyoxal 20.3 μMMiscellaneous78600.0238095

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_21755807670165.31 μM0.352941241186Chembridge (Fragment library)218.27492.03713
SGTC_23307979373200 μM0.3272731863007Chembridge (Fragment library)247.316122.0241460S ribosome export
SGTC_2238667162073.24 μM0.267857729941Chembridge (Fragment library)232.301482.52413
SGTC_2237666774747.22 μM0.245614729940Chembridge (Fragment library)236.2653632.24314
SGTC_3273913785949.47 μM0.2456142377234Chembridge (Drug-like library)266.360764.53803
SGTC_10563448-1962115 μM0.241379715507ChemDiv (Drug-like library)260.311582.55305amide catabolism
SGTC_12360330-0013258 μM0.2413794617066ChemDiv (Drug-like library)262.347381.96212
SGTC_2007401153971.82 μM0.2280745595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_415dibenzyl ketone100 μM0.2222227593Miscellaneous210.271063.29401
SGTC_2922799925732.47 μM0.2203392988377Chembridge (Drug-like library)293.3316263.42614