Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
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YDR328C | 5.63 | 9.04E-9 | 1.69 | SKP1 | Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress |
YPR186C | 3.94 | 4.15E-5 | 0.07 | PZF1 | Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA |
YCR072C | 3.86 | 5.60E-5 | 0.05 | RSA4 | WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus |
YIL003W | 3.81 | 6.90E-5 | 0.16 | CFD1 | Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol |
YHR118C | 3.65 | 1.32E-4 | 0.13 | ORC6 | Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p |
YOL038W | 3.51 | 2.20E-4 | 0.17 | PRE6 | Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress |
YPL169C | 3.35 | 4.09E-4 | 0.18 | MEX67 | Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP |
YDR054C | 3.17 | 7.61E-4 | 0.10 | CDC34 | Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress |
YDL147W | 3.07 | 0.00107 | 0.00 | RPN5 | Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein |
YMR290C | 3.07 | 0.00107 | 0.14 | HAS1 | ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles |
YOR160W | 2.93 | 0.00170 | 0.04 | MTR10 | Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress |
YGR175C | 2.89 | 0.00195 | 0.02 | ERG1 | Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine |
YOR181W | 2.86 | 0.00211 | 0.17 | LAS17 | Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) |
YKL052C | 2.69 | 0.00359 | 0.03 | ASK1 | Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress |
YKL088W | 2.65 | 0.00397 | 0.11 | CAB3 | Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC |