1080-0542

4-[[(E)-3-(4-nitrophenyl)prop-2-enylidene]amino]benzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_567
Screen concentration 68.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 5950415
SMILES C1=CC(=CC=C1C=CC=NC2=CC=C(C=C2)C(=O)O)[N+](=O)[O-]
Standardized SMILES OC(=O)c1ccc(cc1)N=CC=Cc2ccc(cc2)[N+](=O)[O-]
Molecular weight 296.2775
ALogP 3.31
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.98
% growth inhibition (Hom. pool) 12.45


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5950415
Download HIP data (tab-delimited text)  (excel)
Gene:DRE2(YKR071C)|FD-Score:-3.23|P-value:6.10E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:GCD2(YGR083C)|FD-Score:6.21|P-value:2.60E-10|Clearance:1.93||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:PAM18(YLR008C)|FD-Score:4.28|P-value:9.39E-6|Clearance:0.16||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:PGI1(YBR196C)|FD-Score:-3.94|P-value:3.99E-5|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PSF2(YJL072C)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RLP24(YLR009W)|FD-Score:4.12|P-value:1.93E-5|Clearance:0.37||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB3(YIL021W)|FD-Score:3.74|P-value:9.05E-5|Clearance:0.36||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL15A(YLR029C)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN5(YDL147W)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.01||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSA4(YCR072C)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.1||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SQT1(YIR012W)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.1||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SWC4(YGR002C)|FD-Score:-3.11|P-value:9.39E-4|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:ULP1(YPL020C)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:DRE2(YKR071C)|FD-Score:-3.23|P-value:6.10E-4|Clearance:0||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:GCD2(YGR083C)|FD-Score:6.21|P-value:2.60E-10|Clearance:1.93||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:PAM18(YLR008C)|FD-Score:4.28|P-value:9.39E-6|Clearance:0.16||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:PGI1(YBR196C)|FD-Score:-3.94|P-value:3.99E-5|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:PSF2(YJL072C)|FD-Score:-4.22|P-value:1.22E-5|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RLP24(YLR009W)|FD-Score:4.12|P-value:1.93E-5|Clearance:0.37||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB3(YIL021W)|FD-Score:3.74|P-value:9.05E-5|Clearance:0.36||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPL15A(YLR029C)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN5(YDL147W)|FD-Score:3.28|P-value:5.10E-4|Clearance:0.01||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSA4(YCR072C)|FD-Score:3.39|P-value:3.52E-4|Clearance:0.1||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SQT1(YIR012W)|FD-Score:3.18|P-value:7.48E-4|Clearance:0.1||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SWC4(YGR002C)|FD-Score:-3.11|P-value:9.39E-4|Clearance:0||SGD DESC:Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex Gene:ULP1(YPL020C)|FD-Score:-3.66|P-value:1.26E-4|Clearance:0||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5950415
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ALE1(YOR175C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:API2(YDR525W_d)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:AXL1(YPR122W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BNI1(YNL271C)|FD-Score:-4.34|P-value:7.06E-6||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD14(YAR014C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUL2(YML111W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-4.84|P-value:6.48E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.79|P-value:3.53E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.95|P-value:1.36E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF4(YPR135W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYS4(YGR155W)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DEF1(YKL054C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DSD1(YGL196W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:DUF1(YOL087C)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EAF7(YNL136W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECL1(YGR146C)|FD-Score:4.3|P-value:8.53E-6||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ELF1(YKL160W)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:ELM1(YKL048C)|FD-Score:4.96|P-value:3.50E-7||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG28(YER044C)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:ERJ5(YFR041C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAU1(YER183C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GCY1(YOR120W)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRX2(YDR513W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HST3(YOR025W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:IFM1(YOL023W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:MAK10(YEL053C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAP2(YBL091C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MDM34(YGL219C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MMS2(YGL087C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNN10(YDR245W)|FD-Score:-3.87|P-value:5.43E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:NCS2(YNL119W)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NGL2(YMR285C)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NSG1(YHR133C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:PDC1(YLR044C)|FD-Score:5.47|P-value:2.20E-8||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PMT1(YDL095W)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PSY2(YNL201C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RCR1(YBR005W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REG1(YDR028C)|FD-Score:-3.96|P-value:3.77E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RHO4(YKR055W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RNH1(YMR234W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RNH70(YGR276C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:ROM2(YLR371W)|FD-Score:4.31|P-value:8.31E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL14B(YHL001W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL34A(YER056C-A)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTT101(YJL047C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAC7(YDR389W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAF1(YBR280C)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SPT4(YGR063C)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRC1(YML034W)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRL3(YKR091W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SSD1(YDR293C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSZ1(YHR064C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SWF1(YDR126W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TOM6(YOR045W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPN1(YGL186C)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA3(YPR066W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS30(YPL120W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS63(YLR261C_d)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL012C(YBL012C_d)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR001W(YCR001W_d)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR048C(YDR048C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER053C-A(YER053C-A_p)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YMR254C(YMR254C_d)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL184C(YNL184C_p)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL203C(YNL203C_d)|FD-Score:-4.35|P-value:6.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL037C(YOL037C_d)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOR121C(YOR121C_d)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL068C(YPL068C_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR050C(YPR050C_d)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR097W(YPR097W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR170C(YPR170C_d)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT35(YHR105W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZRC1(YMR243C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ADK1(YDR226W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ALE1(YOR175C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:API2(YDR525W_d)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:AXL1(YPR122W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BNI1(YNL271C)|FD-Score:-4.34|P-value:7.06E-6||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD14(YAR014C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUL2(YML111W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CKI1(YLR133W)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-4.84|P-value:6.48E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.79|P-value:3.53E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.95|P-value:1.36E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTF4(YPR135W)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:CYS4(YGR155W)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DEF1(YKL054C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DSD1(YGL196W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:DUF1(YOL087C)|FD-Score:-3.3|P-value:4.88E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EAF7(YNL136W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:ECL1(YGR146C)|FD-Score:4.3|P-value:8.53E-6||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:ELF1(YKL160W)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:ELM1(YKL048C)|FD-Score:4.96|P-value:3.50E-7||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG28(YER044C)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:ERJ5(YFR041C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAU1(YER183C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GCY1(YOR120W)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GET3(YDL100C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRX2(YDR513W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HST3(YOR025W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism Gene:IFM1(YOL023W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:MAK10(YEL053C)|FD-Score:-3.34|P-value:4.19E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MAP2(YBL091C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MDM34(YGL219C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MMS2(YGL087C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNN10(YDR245W)|FD-Score:-3.87|P-value:5.43E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:NCS2(YNL119W)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NGL2(YMR285C)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NSG1(YHR133C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:PDC1(YLR044C)|FD-Score:5.47|P-value:2.20E-8||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PMT1(YDL095W)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PSY2(YNL201C)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RCR1(YBR005W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REG1(YDR028C)|FD-Score:-3.96|P-value:3.77E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RHO4(YKR055W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RNH1(YMR234W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RNH70(YGR276C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:ROM2(YLR371W)|FD-Score:4.31|P-value:8.31E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL14B(YHL001W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL34A(YER056C-A)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RRG9(YNL213C)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTT101(YJL047C)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SAC7(YDR389W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SAF1(YBR280C)|FD-Score:-3.14|P-value:8.46E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SPT4(YGR063C)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRC1(YML034W)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SRL3(YKR091W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SSD1(YDR293C)|FD-Score:-3.25|P-value:5.79E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSZ1(YHR064C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:SWF1(YDR126W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TOM6(YOR045W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TPN1(YGL186C)|FD-Score:-3.44|P-value:2.92E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBA3(YPR066W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS30(YPL120W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS63(YLR261C_d)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS71(YML041C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBL012C(YBL012C_d)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR001W(YCR001W_d)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.49|P-value:3.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR048C(YDR048C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER053C-A(YER053C-A_p)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YMR254C(YMR254C_d)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL184C(YNL184C_p)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL203C(YNL203C_d)|FD-Score:-4.35|P-value:6.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL037C(YOL037C_d)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YOR121C(YOR121C_d)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL068C(YPL068C_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR050C(YPR050C_d)|FD-Score:-3.96|P-value:3.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR097W(YPR097W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR170C(YPR170C_d)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT35(YHR105W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZRC1(YMR243C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR083C6.212.60E-101.93GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YLR008C4.289.39E-60.16PAM18Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
YLR009W4.121.93E-50.37RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YIL021W3.749.05E-50.36RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YCR072C3.393.52E-40.10RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YDL147W3.285.10E-40.01RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YLR029C3.275.35E-40.10RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YIR012W3.187.48E-40.10SQT1Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
YNL247W3.080.001050.01YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YOR146W_d3.070.001070.05YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YBL097W3.020.001260.06BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YLR075W2.960.001540.00RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YHR188C2.950.001560.07GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YOL038W2.880.001980.01PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YHR186C2.870.002070.01KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR044C5.472.20E-8PDC1Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
YKL048C4.963.50E-7ELM1Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
YNL119W4.681.45E-6NCS2Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
YML034W4.631.85E-6SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YGR155W4.582.32E-6CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YPL120W4.533.00E-6VPS30Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
YCR085W_d4.493.62E-6YCR085W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YNL213C4.386.06E-6RRG9Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBL012C_d4.337.34E-6YBL012C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR371W4.318.31E-6ROM2GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p
YGR146C4.308.53E-6ECL1Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
YJL047C3.963.71E-5RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YKL160W3.817.03E-5ELF1Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
YER053C-A_p3.768.52E-5YER053C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YPR170C_d3.758.96E-5YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

GO enrichment analysis for SGTC_567
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1286.85E-23SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0606061TSC3-RPN4
0.1102.69E-17SGTC_5643555-0129 330.0 μMChemDiv (Drug-like library)5632600.0555556
0.1071.66E-16SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.179104
0.1017.68E-15SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.184615TSC3-RPN4
0.0983.52E-14SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.0722892TSC3-RPN4
0.0978.13E-14SGTC_13321480-0653 17.5 μMChemDiv (Drug-like library)57494380.109375TSC3-RPN4
0.0897.89E-12SGTC_1845st056753 3.0 μMTimTec (Natural product derivative library)53758390.20339TSC3-RPN4
0.0881.04E-11SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0806452TSC3-RPN4
0.0863.60E-11SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.0434783SWF1 & branched chain AA biosynthesis
0.0848.47E-11SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0298507TSC3-RPN4
0.0831.43E-10SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.115942TSC3-RPN4
0.0831.61E-10SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.101695heme biosynthesis & mitochondrial translocase
0.0831.81E-10SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0533333TSC3-RPN4
0.0822.21E-10SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.2SWF1 & branched chain AA biosynthesis
0.0813.97E-10SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.08fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8610443-0273110 μM0.7142866149169ChemDiv (Drug-like library)268.267383.43914
SGTC_840443-0269128.82 μM0.551026150058ChemDiv (Drug-like library)266.294564.16703
SGTC_1410443-027432.33 μM0.5416671627241ChemDiv (Drug-like library)268.267383.43914mitochondrial response to ROS
SGTC_24005203494178.7 μM0.4393946777674Miscellaneous399.398784.55216RSC complex & mRNA processing
SGTC_1813st0512468.6 μM0.43755291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_3690957-03934.5 μM0.4328366997992ChemDiv (Drug-like library)413.425365.03816cell wall signaling
SGTC_580957-03939 μM0.4328366997992ChemDiv (Drug-like library)413.425365.03816cell wall
SGTC_12570669-012116 μM0.4038464307855ChemDiv (Drug-like library)325.365.16513
SGTC_6880527-0193473 μM0.3928574007154ChemDiv (Drug-like library)286.24293.10827fatty acid desaturase (OLE1)
SGTC_9262268-140159.4 μM0.3728812931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_2350330-00875 mM0.36842146494818ChemDiv (Drug-like library)444.653284.76806TSC3-RPN4