0960-0087

N-[3-[[3-[(2-chlorophenyl)hydrazinylidene]-6-oxocyclohexa-1,4-dien-1-yl]methylideneamino]phenyl]furan-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_572
Screen concentration 17.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 6796365
SMILES C1=CC=C(C(=C1)NN=C2C=CC(=O)C(=C2)C=NC3=CC(=CC=C3)NC(=O)C4=CC=CO4)Cl
Standardized SMILES Oc1ccc(cc1C=Nc2cccc(NC(=O)c3occc3)c2)N=Nc4ccccc4Cl
Molecular weight 444.8698
ALogP 4.84
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.11
% growth inhibition (Hom. pool) 6.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6796365
Download HIP data (tab-delimited text)  (excel)
Gene:ARC35(YNR035C)|FD-Score:-4.15|P-value:1.64E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CDC12(YHR107C)|FD-Score:5.85|P-value:2.42E-9|Clearance:0.83||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC8(YJR057W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.18||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CET1(YPL228W)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.02||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:COP1(YDL145C)|FD-Score:3.16|P-value:7.80E-4|Clearance:0.02||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:HEM4(YOR278W)|FD-Score:4.7|P-value:1.29E-6|Clearance:0.27||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSH49(YOR319W)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.03||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MCM3(YEL032W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.03||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NUP159(YIL115C)|FD-Score:3.1|P-value:9.78E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:POP4(YBR257W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.13||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP3(YDR473C)|FD-Score:4.36|P-value:6.41E-6|Clearance:0.03||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP9(YDL030W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.04||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:REB1(YBR049C)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.14||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPB5(YBR154C)|FD-Score:3.2|P-value:6.96E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RRP14(YKL082C)|FD-Score:-3.17|P-value:7.52E-4|Clearance:0||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC58(YLR033W)|FD-Score:5.03|P-value:2.49E-7|Clearance:0.32||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC3(YER008C)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SEC31(YDL195W)|FD-Score:9.72|P-value:1.23E-22|Clearance:3.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SFH1(YLR321C)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.28||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SRP14(YDL092W)|FD-Score:4.34|P-value:7.28E-6|Clearance:0.12||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:SUP45(YBR143C)|FD-Score:6.68|P-value:1.18E-11|Clearance:0.83||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TSC10(YBR265W)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.03||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UTP13(YLR222C)|FD-Score:4.43|P-value:4.67E-6|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VAS1(YGR094W)|FD-Score:4.71|P-value:1.24E-6|Clearance:0.01||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YLR230W(YLR230W_d)|FD-Score:3.89|P-value:4.94E-5|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:ARC35(YNR035C)|FD-Score:-4.15|P-value:1.64E-5|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CDC12(YHR107C)|FD-Score:5.85|P-value:2.42E-9|Clearance:0.83||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC8(YJR057W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.18||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:CET1(YPL228W)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.02||SGD DESC:Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide Gene:COP1(YDL145C)|FD-Score:3.16|P-value:7.80E-4|Clearance:0.02||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:HEM4(YOR278W)|FD-Score:4.7|P-value:1.29E-6|Clearance:0.27||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:HSH49(YOR319W)|FD-Score:3.22|P-value:6.34E-4|Clearance:0.03||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MCM3(YEL032W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.03||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NUP159(YIL115C)|FD-Score:3.1|P-value:9.78E-4|Clearance:0.01||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:POP4(YBR257W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.13||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP3(YDR473C)|FD-Score:4.36|P-value:6.41E-6|Clearance:0.03||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PRP9(YDL030W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.04||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:REB1(YBR049C)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.14||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPB5(YBR154C)|FD-Score:3.2|P-value:6.96E-4|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RRP14(YKL082C)|FD-Score:-3.17|P-value:7.52E-4|Clearance:0||SGD DESC:Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family Gene:RSC58(YLR033W)|FD-Score:5.03|P-value:2.49E-7|Clearance:0.32||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC3(YER008C)|FD-Score:-3.57|P-value:1.77E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SEC31(YDL195W)|FD-Score:9.72|P-value:1.23E-22|Clearance:3.04||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SFH1(YLR321C)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.28||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SRP14(YDL092W)|FD-Score:4.34|P-value:7.28E-6|Clearance:0.12||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:SUP45(YBR143C)|FD-Score:6.68|P-value:1.18E-11|Clearance:0.83||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TSC10(YBR265W)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.03||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UTP13(YLR222C)|FD-Score:4.43|P-value:4.67E-6|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VAS1(YGR094W)|FD-Score:4.71|P-value:1.24E-6|Clearance:0.01||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YLR230W(YLR230W_d)|FD-Score:3.89|P-value:4.94E-5|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6796365
Download HOP data (tab-delimited text)  (excel)
Gene:AIM44(YPL158C)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ARI1(YGL157W)|FD-Score:4.81|P-value:7.59E-7||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARP1(YHR129C)|FD-Score:4.8|P-value:8.03E-7||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ARR3(YPR201W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG2(YNL242W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATP14(YLR295C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AYR1(YIL124W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BEM1(YBR200W)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BNA1(YJR025C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BNA4(YBL098W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CBC2(YPL178W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCC2(YDR270W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CIT3(YPR001W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CMR3(YPR013C_p)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COG6(YNL041C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:7.55|P-value:2.25E-14||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CRG1(YHR209W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:EAF7(YNL136W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EIS1(YMR031C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ELG1(YOR144C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMC1(YCL045C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FBP26(YJL155C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FMP52(YER004W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FYV1(YDR024W_d)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GRX7(YBR014C)|FD-Score:6.12|P-value:4.82E-10||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOP2(YGL033W)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IRC18(YJL037W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC19(YLL033W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC25(YLR021W)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:MAL13(YGR288W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAM1(YER106W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MET10(YFR030W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET8(YBR213W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MGT1(YDL200C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNL1(YHR204W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MOT3(YMR070W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPC54(YOR177C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL20(YKR085C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSG5(YNL053W)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSW1(YDR268W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NOP13(YNL175C)|FD-Score:4.83|P-value:6.84E-7||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NST1(YNL091W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PEP7(YDR323C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PHD1(YKL043W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PIP2(YOR363C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PMT1(YDL095W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:POS5(YPL188W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PPN1(YDR452W)|FD-Score:4.29|P-value:8.83E-6||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PUF4(YGL014W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PUG1(YER185W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RTK1(YDL025C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SAP1(YER047C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SEA4(YBL104C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SER1(YOR184W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SKN7(YHR206W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SPS2(YDR522C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SSA3(YBL075C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SSP2(YOR242C)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:STB2(YMR053C)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SUT2(YPR009W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TAT1(YBR069C)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO4(YOR273C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:6.44|P-value:5.97E-11||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TYE7(YOR344C)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UME1(YPL139C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:URC2(YDR520C_p)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAC7(YNL054W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS61(YDR136C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL053W(YBL053W_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR064C(YCR064C_d)|FD-Score:-5.77|P-value:3.89E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL026W(YDL026W_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL073W(YDL073W_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDR444W(YDR444W_p)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR455C(YDR455C_d)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEF1(YEL041W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YEL028W(YEL028W_d)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YKR047W(YKR047W_d)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YKR051W(YKR051W_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative protein of unknown function Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function Gene:YLR455W(YLR455W_p)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML096W(YML096W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR144W(YMR144W_p)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR365C(YOR365C_p)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPR050C(YPR050C_d)|FD-Score:5.29|P-value:6.25E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YRR1(YOR162C)|FD-Score:6.66|P-value:1.35E-11||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:AIM44(YPL158C)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ARI1(YGL157W)|FD-Score:4.81|P-value:7.59E-7||SGD DESC:NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily Gene:ARP1(YHR129C)|FD-Score:4.8|P-value:8.03E-7||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ARR3(YPR201W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG2(YNL242W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress Gene:ATP14(YLR295C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:AYR1(YIL124W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BEM1(YBR200W)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BNA1(YJR025C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BNA4(YBL098W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CBC2(YPL178W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCC2(YDR270W)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CIT3(YPR001W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CMR3(YPR013C_p)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:COG6(YNL041C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:7.55|P-value:2.25E-14||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CRG1(YHR209W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:EAF7(YNL136W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:EIS1(YMR031C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ELG1(YOR144C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMC1(YCL045C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FBP26(YJL155C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FMP52(YER004W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FYV1(YDR024W_d)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GRX7(YBR014C)|FD-Score:6.12|P-value:4.82E-10||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOP2(YGL033W)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IRC18(YJL037W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC19(YLL033W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC25(YLR021W)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:MAL13(YGR288W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MAM1(YER106W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MET10(YFR030W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET8(YBR213W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MGT1(YDL200C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MNL1(YHR204W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MOT3(YMR070W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MPC54(YOR177C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRPL20(YKR085C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSG5(YNL053W)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSW1(YDR268W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NOP13(YNL175C)|FD-Score:4.83|P-value:6.84E-7||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NST1(YNL091W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:PEP7(YDR323C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PHD1(YKL043W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PIP2(YOR363C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PMT1(YDL095W)|FD-Score:4.55|P-value:2.66E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:POS5(YPL188W)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PPN1(YDR452W)|FD-Score:4.29|P-value:8.83E-6||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PUF4(YGL014W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:PUG1(YER185W)|FD-Score:5.16|P-value:1.23E-7||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RTK1(YDL025C)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SAP1(YER047C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SEA4(YBL104C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SER1(YOR184W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SKN7(YHR206W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SPS2(YDR522C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SSA3(YBL075C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSA4(YER103W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SSP2(YOR242C)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; SSP2 expression is induced midway in meiosis Gene:STB2(YMR053C)|FD-Score:3.89|P-value:5.07E-5||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SUT2(YPR009W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TAT1(YBR069C)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO4(YOR273C)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TSC3(YBR058C-A)|FD-Score:6.44|P-value:5.97E-11||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TYE7(YOR344C)|FD-Score:-4.64|P-value:1.76E-6||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:UME1(YPL139C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:URC2(YDR520C_p)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAC7(YNL054W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS61(YDR136C_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBL053W(YBL053W_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR064C(YCR064C_d)|FD-Score:-5.77|P-value:3.89E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL026W(YDL026W_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL073W(YDL073W_p)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDR444W(YDR444W_p)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR455C(YDR455C_d)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEF1(YEL041W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YEL028W(YEL028W_d)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER097W(YER097W_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YKR047W(YKR047W_d)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YKR051W(YKR051W_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative protein of unknown function Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function Gene:YLR455W(YLR455W_p)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML096W(YML096W_p)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR144W(YMR144W_p)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YNR025C(YNR025C_d)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR365C(YOR365C_p)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YPR050C(YPR050C_d)|FD-Score:5.29|P-value:6.25E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YRR1(YOR162C)|FD-Score:6.66|P-value:1.35E-11||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W9.721.23E-223.04SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBR143C6.681.18E-110.83SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YHR107C5.852.42E-90.83CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR033W5.032.49E-70.32RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YGR094W4.711.24E-60.01VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YOR278W4.701.29E-60.27HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YLR222C4.434.67E-60.07UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR473C4.366.41E-60.03PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YDL092W4.347.28E-60.12SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YBR049C4.211.27E-50.14REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YJR057W4.072.34E-50.18CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YLR230W_d3.894.94E-50.20YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YBR257W3.691.11E-40.13POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YDL030W3.571.80E-40.04PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YLR321C3.532.07E-40.28SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL005C7.552.25E-14COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOR162C6.661.35E-11YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YBR058C-A6.445.97E-11TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YBR014C6.124.82E-10GRX7Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
YPR050C_d5.296.25E-8YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
YER185W5.161.23E-7PUG1Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
YML071C4.993.06E-7COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL175C4.836.84E-7NOP13Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
YLR455W_p4.827.02E-7YLR455W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress
YGL157W4.817.59E-7ARI1NADPH-dependent aldehyde reductase, utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
YHR129C4.808.03E-7ARP1Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
YDR520C_p4.751.02E-6URC2_pPutative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
YDL200C4.741.06E-6MGT1DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage
YBL104C4.721.16E-6SEA4Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
YGL033W4.631.84E-6HOP2Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair

GO enrichment analysis for SGTC_572
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1717.72E-40SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.725806
0.1672.51E-38SGTC_8550428-0020 25.0 μMChemDiv (Drug-like library)68124140.34375Golgi
0.1591.21E-34SGTC_590k048-0076 22.4 μMChemDiv (Drug-like library)68328140.25RSC & ERG11
0.1482.96E-30SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.09375
0.1381.21E-26SGTC_1870st057514 26.5 μMTimTec (Natural product derivative library)53931610.117647RSC & ERG11
0.1361.10E-25SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0229885TSC3-RPN4
0.1352.41E-25SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.384615RSC & ERG11
0.1344.30E-25SGTC_342k048-0088 23.3 μMChemDiv (Drug-like library)68205700.192771RSC & ERG11
0.1344.55E-25SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.101124
0.1331.15E-24SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.218391Golgi
0.1331.28E-24SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.226667Golgi
0.1322.61E-24SGTC_5660958-0068 16.5 μMChemDiv (Drug-like library)68153650.371795RSC & ERG11
0.1323.38E-24SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0449438
0.1316.78E-24SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.191011TSC3-RPN4
0.1301.59E-23SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0470588TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8940960-023855.9 μM0.7258066741907ChemDiv (Drug-like library)340.760422.64724
SGTC_40960-023425.67 μM0.5652176821112ChemDiv (Drug-like library)558.108422.84424Golgi
SGTC_13301469-005461.8 μM0.4675327102664ChemDiv (Drug-like library)382.80372.93536iron homeostasis
SGTC_4300958-011516.4 μM0.4594596833180ChemDiv (Drug-like library)508.590424.45323
SGTC_20635246145200 μM0.396825711240Chembridge (Fragment library)221.639722.67412
SGTC_20255147754141 μM0.393939315777Chembridge (Fragment library)229.231341.74913
SGTC_9701315-008919.5 μM0.3846156811083ChemDiv (Drug-like library)582.172883.93523RSC & ERG11
SGTC_4271354-005347.3 μM0.3783786813657ChemDiv (Drug-like library)407.892684.19625
SGTC_5660958-006816.5 μM0.3717956815365ChemDiv (Drug-like library)492.1358233.99424RSC & ERG11
SGTC_1642st00958277.7 μM0.3529416739311TimTec (Natural product derivative library)257.241441.48735RPP1 & pyrimidine depletion