3702-0553

1,4-bis[(3-bromophenyl)methyl]piperazine

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_573
Screen concentration 84.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 1222756
SMILES C1CN(CCN1CC2=CC(=CC=C2)Br)CC3=CC(=CC=C3)Br
Standardized SMILES Brc1cccc(CN2CCN(Cc3cccc(Br)c3)CC2)c1
Molecular weight 424.1728
ALogP 4.94
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.42
% growth inhibition (Hom. pool) 1.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1222756
Download HIP data (tab-delimited text)  (excel)
Gene:AOS1(YPR180W)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.18||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:ERG7(YHR072W)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GAB1(YLR459W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.05||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPI12(YMR281W)|FD-Score:-5|P-value:2.80E-7|Clearance:0||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:KEI1(YDR367W)|FD-Score:-3.36|P-value:3.90E-4|Clearance:0||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LUC7(YDL087C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.02||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MIA40(YKL195W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.11||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAN1(YPL126W)|FD-Score:-3.29|P-value:4.98E-4|Clearance:0||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NOB1(YOR056C)|FD-Score:3.75|P-value:8.67E-5|Clearance:0.1||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:PIS1(YPR113W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.19||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP4(YBR257W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPC34(YNR003C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RPT4(YOR259C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.03||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SAD1(YFR005C)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.02||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SEC8(YPR055W)|FD-Score:-3.1|P-value:9.69E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:TAF11(YML015C)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.03||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:YAH1(YPL252C)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.42||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:AOS1(YPR180W)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.18||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:ERG7(YHR072W)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GAB1(YLR459W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.05||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:GPI12(YMR281W)|FD-Score:-5|P-value:2.80E-7|Clearance:0||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:KEI1(YDR367W)|FD-Score:-3.36|P-value:3.90E-4|Clearance:0||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LUC7(YDL087C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.02||SGD DESC:Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA Gene:MIA40(YKL195W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.11||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NAN1(YPL126W)|FD-Score:-3.29|P-value:4.98E-4|Clearance:0||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NOB1(YOR056C)|FD-Score:3.75|P-value:8.67E-5|Clearance:0.1||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:PIS1(YPR113W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0.19||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:POP4(YBR257W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPC34(YNR003C)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RPT4(YOR259C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.03||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SAD1(YFR005C)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.02||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SEC8(YPR055W)|FD-Score:-3.1|P-value:9.69E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:TAF11(YML015C)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.03||SGD DESC:TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors Gene:YAH1(YPL252C)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.42||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1222756
Download HOP data (tab-delimited text)  (excel)
Gene:ATG11(YPR049C)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG18(YFR021W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BAR1(YIL015W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BIO2(YGR286C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BIO5(YNR056C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CLC1(YGR167W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DAL1(YIR027C)|FD-Score:3.76|P-value:8.45E-5||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIG1(YPL049C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:EAF5(YEL018W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:EIS1(YMR031C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:FLD1(YLR404W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP16(YDR070C_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FMP37(YGL080W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GEA1(YJR031C)|FD-Score:-3.74|P-value:9.13E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GPD2(YOL059W)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:ICY1(YMR195W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IDP1(YDL066W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IOC3(YFR013W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC19(YLL033W)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:MET3(YJR010W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MFA1(YDR461W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MUS81(YDR386W)|FD-Score:4.27|P-value:9.96E-6||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPL4(YBR170C)|FD-Score:-3.78|P-value:7.88E-5||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:NUP53(YMR153W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OMS1(YDR316W)|FD-Score:4.29|P-value:9.04E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PEX12(YMR026C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHO91(YNR013C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PIH1(YHR034C)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMT1(YDL095W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPN1(YDR452W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PPZ1(YML016C)|FD-Score:5.04|P-value:2.27E-7||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD24(YER173W)|FD-Score:4.41|P-value:5.05E-6||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RPL14B(YHL001W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS10B(YMR230W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Mitochondrial protein of unknown function Gene:TAL1(YLR354C)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TPN1(YGL186C)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBA3(YPR066W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL029W(YBL029W_p)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Non-essential protein of unknown function Gene:YCR087W(YCR087W_d)|FD-Score:5.3|P-value:5.67E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR415C(YDR415C_p)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Putative aminopeptidase Gene:YGL072C(YGL072C_d)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL185C(YGL185C_p)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGP1(YNL160W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR242W(YGR242W_d)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL211C(YJL211C_d)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR118C(YLR118C)|FD-Score:-4.33|P-value:7.56E-6||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR194C(YLR194C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YML096W(YML096W_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR31(YFR049W)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YND1(YER005W)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function Gene:YNR073C(YNR073C_p)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL114C(YOL114C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:ATG11(YPR049C)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG18(YFR021W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:BAR1(YIL015W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BIO2(YGR286C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BIO5(YNR056C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CLC1(YGR167W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DAL1(YIR027C)|FD-Score:3.76|P-value:8.45E-5||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIG1(YPL049C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:EAF5(YEL018W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Esa1p-associated factor, non-essential subunit of the NuA4 acetyltransferase complex Gene:EIS1(YMR031C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:FLD1(YLR404W)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FMP16(YDR070C_p)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:FMP37(YGL080W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GEA1(YJR031C)|FD-Score:-3.74|P-value:9.13E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GPD2(YOL059W)|FD-Score:4.41|P-value:5.12E-6||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:ICY1(YMR195W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IDP1(YDL066W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IOC3(YFR013W)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC19(YLL033W)|FD-Score:-4.4|P-value:5.42E-6||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:MET3(YJR010W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MFA1(YDR461W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MUS81(YDR386W)|FD-Score:4.27|P-value:9.96E-6||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPL4(YBR170C)|FD-Score:-3.78|P-value:7.88E-5||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:NUP53(YMR153W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:OMS1(YDR316W)|FD-Score:4.29|P-value:9.04E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PEX12(YMR026C)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHO91(YNR013C)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PIH1(YHR034C)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMT1(YDL095W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPN1(YDR452W)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PPZ1(YML016C)|FD-Score:5.04|P-value:2.27E-7||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD24(YER173W)|FD-Score:4.41|P-value:5.05E-6||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RPL14B(YHL001W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS10B(YMR230W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Mitochondrial protein of unknown function Gene:TAL1(YLR354C)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TPN1(YGL186C)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:UBA3(YPR066W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL029W(YBL029W_p)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Non-essential protein of unknown function Gene:YCR087W(YCR087W_d)|FD-Score:5.3|P-value:5.67E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR415C(YDR415C_p)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Putative aminopeptidase Gene:YGL072C(YGL072C_d)|FD-Score:3.8|P-value:7.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL185C(YGL185C_p)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGP1(YNL160W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR242W(YGR242W_d)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YJL211C(YJL211C_d)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR118C(YLR118C)|FD-Score:-4.33|P-value:7.56E-6||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR194C(YLR194C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YML096W(YML096W_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR31(YFR049W)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YND1(YER005W)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function Gene:YNR073C(YNR073C_p)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Putative mannitol dehydrogenase; identical in amino acid sequence to Dsf1p Gene:YOL114C(YOL114C_p)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL252C4.231.17E-50.42YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YML015C3.816.94E-50.03TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YFR005C3.787.96E-50.02SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YOR056C3.758.67E-50.10NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YMR290W-A_d3.661.28E-40.08YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YBR257W3.581.71E-40.02POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YLR459W3.561.85E-40.05GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
YKL195W3.522.19E-40.11MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YOR259C3.413.25E-40.03RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YPR113W3.383.68E-40.19PIS1Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
YDL087C3.197.16E-40.02LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YPR180W3.167.76E-40.18AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YNL152W2.990.001410.08INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YLR409C2.910.001800.00UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YKL172W2.910.001820.00EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR049C5.383.68E-8ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)
YCR087W_d5.305.67E-8YCR087W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene
YML016C5.042.27E-7PPZ1Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
YHR034C4.963.46E-7PIH1Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
YGL186C4.562.55E-6TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YNR056C4.444.49E-6BIO5Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
YER173W4.415.05E-6RAD24Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
YOL059W4.415.12E-6GPD2NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1
YDR316W4.299.04E-6OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YDR386W4.279.96E-6MUS81Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
YDR070C_p4.171.51E-5FMP16_pProtein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
YNR013C4.052.52E-5PHO91Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
YGL080W3.993.31E-5FMP37Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia
YDR452W3.973.66E-5PPN1Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
YIL015W3.895.00E-5BAR1Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest

GO enrichment analysis for SGTC_573
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0713.82E-8SGTC_5741315-0397 40.0 μMChemDiv (Drug-like library)67942770.126761
0.0681.68E-7SGTC_10462001-0062 216.0 μMChemDiv (Drug-like library)7397780.0757576
0.0682.09E-7SGTC_244nsc-207895 1.3 μMMiscellaneous426400.101695DNA damage response
0.0631.39E-6SGTC_1083didemethylchlorpromazine sulfoxide 707.4 nMTimTec (Natural product library)3987880.107143redox potentiating
0.0622.05E-6SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0588235heme biosynthesis & mitochondrial translocase
0.0613.22E-6SGTC_33529151559 45.6 μMChembridge (Drug-like library)171593390.126984
0.0604.11E-6SGTC_30579095946 49.5 μMChembridge (Drug-like library)245400430.118644Golgi
0.0594.85E-6SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.0816327
0.0571.19E-5SGTC_8600443-0016 25.1 μMChemDiv (Drug-like library)53379420.0784314
0.0571.28E-5SGTC_15494'-methoxyflavonol 74.6 μMTimTec (Pure natural product library)971410.0727273
0.0552.40E-5SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.0740741
0.0552.75E-5SGTC_12001643-0193 18.4 μMChemDiv (Drug-like library)54039740.101449
0.0543.75E-5SGTC_8530419-0306 21.0 μMChemDiv (Drug-like library)7627130.14
0.0535.25E-5SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.18181860S ribosome export
0.0525.91E-5SGTC_1964st076914 61.0 μMTimTec (Natural product derivative library)242086610.112903RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6583253-0951102 μM0.551184878ChemDiv (Drug-like library)358.27553.85303calcium & mitochondrial duress
SGTC_5154092-1064102 μM0.529412717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_3158910000749.47 μM0.48837219297233Chembridge (Drug-like library)373.286844.01902
SGTC_1784092-080687.51 μM0.4864862169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_2049526283167.19 μM0.439024763417Chembridge (Fragment library)272.3206832.4805
SGTC_1818st05213656.3 μM0.4674169TimTec (Natural product derivative library)339.861784.40212NEO1-PIK1
SGTC_13752910-0797186 μM0.391304742508ChemDiv (Drug-like library)340.416162.75204
SGTC_14394092-116846 μM0.3829792886240ChemDiv (Drug-like library)346.851123.8514
SGTC_3045909295049.47 μM0.38297944120432Chembridge (Drug-like library)365.296784.31712
SGTC_3049909222349.47 μM0.3829798623917Chembridge (Drug-like library)328.835843.93502