4491-1009

2-methyl-N-phenylbenzo[h]quinolin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_579
Screen concentration 110.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1271041
SMILES CC1=NC2=C(C=CC3=CC=CC=C32)C(=C1)NC4=CC=CC=C4
Standardized SMILES Cc1cc(Nc2ccccc2)c3ccc4ccccc4c3n1
Molecular weight 284.3544
ALogP 4.76
H-bond donor count 1
H-bond acceptor count 2
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 29.07
% growth inhibition (Hom. pool) 10.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1271041
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.12||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:BET3(YKR068C)|FD-Score:5.54|P-value:1.50E-8|Clearance:0.63||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CCT2(YIL142W)|FD-Score:3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:4.06|P-value:2.43E-5|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:4.42|P-value:4.91E-6|Clearance:0.36||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC24(YAL041W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.13||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CWC2(YDL209C)|FD-Score:3.25|P-value:5.87E-4|Clearance:0.02||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GWT1(YJL091C)|FD-Score:3.93|P-value:4.29E-5|Clearance:0.19||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:IPI3(YNL182C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.03||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:LSG1(YGL099W)|FD-Score:13.1|P-value:1.61E-39|Clearance:5.38||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS2(YHR024C)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.02||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NMD3(YHR170W)|FD-Score:7.72|P-value:5.87E-15|Clearance:2.18||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PBN1(YCL052C)|FD-Score:4.92|P-value:4.42E-7|Clearance:0.49||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PRE6(YOL038W)|FD-Score:-3.11|P-value:9.26E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP2(YNR011C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RPA190(YOR341W)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.08||SGD DESC:RNA polymerase I largest subunit A190 Gene:TIF6(YPR016C)|FD-Score:3.38|P-value:3.69E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIM54(YJL054W)|FD-Score:-3.85|P-value:6.02E-5|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOM22(YNL131W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TSC13(YDL015C)|FD-Score:3.74|P-value:9.26E-5|Clearance:0.1||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:VAS1(YGR094W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.14||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YJU2(YKL095W)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.1||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:APC5(YOR249C)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.12||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:BET3(YKR068C)|FD-Score:5.54|P-value:1.50E-8|Clearance:0.63||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:CCT2(YIL142W)|FD-Score:3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:4.06|P-value:2.43E-5|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:4.42|P-value:4.91E-6|Clearance:0.36||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDC24(YAL041W)|FD-Score:3.37|P-value:3.74E-4|Clearance:0.13||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CWC2(YDL209C)|FD-Score:3.25|P-value:5.87E-4|Clearance:0.02||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:GWT1(YJL091C)|FD-Score:3.93|P-value:4.29E-5|Clearance:0.19||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:IPI3(YNL182C)|FD-Score:3.14|P-value:8.53E-4|Clearance:0.03||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:LSG1(YGL099W)|FD-Score:13.1|P-value:1.61E-39|Clearance:5.38||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MAS2(YHR024C)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.02||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:NMD3(YHR170W)|FD-Score:7.72|P-value:5.87E-15|Clearance:2.18||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PBN1(YCL052C)|FD-Score:4.92|P-value:4.42E-7|Clearance:0.49||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PRE6(YOL038W)|FD-Score:-3.11|P-value:9.26E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP2(YNR011C)|FD-Score:3.14|P-value:8.40E-4|Clearance:0||SGD DESC:RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16 Gene:RPA190(YOR341W)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.08||SGD DESC:RNA polymerase I largest subunit A190 Gene:TIF6(YPR016C)|FD-Score:3.38|P-value:3.69E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIM54(YJL054W)|FD-Score:-3.85|P-value:6.02E-5|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOM22(YNL131W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TSC13(YDL015C)|FD-Score:3.74|P-value:9.26E-5|Clearance:0.1||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:VAS1(YGR094W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.14||SGD DESC:Mitochondrial and cytoplasmic valyl-tRNA synthetase Gene:YJU2(YKL095W)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.1||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1271041
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AEP2(YMR282C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ARO7(YPR060C)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARO9(YHR137W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:BFR1(YOR198C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BRO1(YPL084W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CAP2(YIL034C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:5.39|P-value:3.53E-8||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:COX9(YDL067C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYS3(YAL012W)|FD-Score:-3.8|P-value:7.23E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EFM1(YHL039W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELP6(YMR312W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FCY1(YPR062W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FUI1(YBL042C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV12(YOR183W_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAS1(YMR307W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GOT1(YMR292W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:HIS4(YCL030C)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HOR7(YMR251W-A)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HSL7(YBR133C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HVG1(YER039C)|FD-Score:4.76|P-value:9.84E-7||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IST1(YNL265C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:JJJ3(YJR097W)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KNS1(YLL019C)|FD-Score:-4.34|P-value:7.10E-6||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LSC2(YGR244C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAK10(YEL053C)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MET17(YLR303W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MLS1(YNL117W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL11(YDL202W)|FD-Score:6.5|P-value:4.04E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL17(YNL252C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MTC7(YEL033W_p)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUB1(YMR100W)|FD-Score:-4.31|P-value:8.02E-6||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:MUP1(YGR055W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:MXR1(YER042W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NUP100(YKL068W)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:PAC10(YGR078C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAT1(YCR077C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGC1(YPL206C)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:RAD16(YBR114W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD61(YDR014W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RBL2(YOR265W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RPL22A(YLR061W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL23A(YBL087C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:SAE2(YGL175C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAS5(YOR213C)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCO1(YBR037C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCW11(YGL028C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SHH4(YLR164W)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SIS2(YKR072C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:TIF2(YJL138C)|FD-Score:-3.9|P-value:4.71E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TMA19(YKL056C)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPN1(YGL186C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSR3(YOR006C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:URA10(YMR271C)|FD-Score:5.36|P-value:4.17E-8||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:WTM2(YOR229W)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1(YML007W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL183C(YDL183C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR509W(YDR509W_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER053C-A(YER053C-A_p)|FD-Score:5.78|P-value:3.81E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YFR032C-B(YFR032C-B_p)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL088W(YGL088W_d)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIL055C(YIL055C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL169W(YJL169W_d)|FD-Score:-5.23|P-value:8.31E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YLL047W(YLL047W_d)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YMR052C-A(YMR052C-A_d)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR074C(YMR074C)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR262W(YMR262W_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YOR1(YGR281W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:ZUO1(YGR285C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:ACE2(YLR131C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AEP2(YMR282C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:ARO7(YPR060C)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARO9(YHR137W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:BFR1(YOR198C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BRO1(YPL084W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:CAP2(YIL034C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX19(YLL018C-A)|FD-Score:5.39|P-value:3.53E-8||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:COX9(YDL067C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYS3(YAL012W)|FD-Score:-3.8|P-value:7.23E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EFM1(YHL039W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELP6(YMR312W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FCY1(YPR062W)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FUI1(YBL042C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV12(YOR183W_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAS1(YMR307W)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GOT1(YMR292W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:HIS4(YCL030C)|FD-Score:3.77|P-value:8.04E-5||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HOR7(YMR251W-A)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HSL7(YBR133C)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HVG1(YER039C)|FD-Score:4.76|P-value:9.84E-7||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IST1(YNL265C)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p Gene:JJJ3(YJR097W)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KNS1(YLL019C)|FD-Score:-4.34|P-value:7.10E-6||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LSC2(YGR244C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Beta subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate Gene:MAK10(YEL053C)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MET17(YLR303W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MLS1(YNL117W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MRPL11(YDL202W)|FD-Score:6.5|P-value:4.04E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL17(YNL252C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MTC7(YEL033W_p)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUB1(YMR100W)|FD-Score:-4.31|P-value:8.02E-6||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:MUP1(YGR055W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:MXR1(YER042W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NUP100(YKL068W)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:PAC10(YGR078C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAT1(YCR077C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGC1(YPL206C)|FD-Score:3.72|P-value:9.88E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:RAD16(YBR114W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD61(YDR014W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RBL2(YOR265W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress Gene:RPL22A(YLR061W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL23A(YBL087C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:SAE2(YGL175C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAS5(YOR213C)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SCO1(YBR037C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SCW11(YGL028C)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SHH4(YLR164W)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SIS2(YKR072C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:TIF2(YJL138C)|FD-Score:-3.9|P-value:4.71E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TMA19(YKL056C)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TPN1(YGL186C)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSR3(YOR006C)|FD-Score:3.81|P-value:6.98E-5||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:URA10(YMR271C)|FD-Score:5.36|P-value:4.17E-8||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:WTM2(YOR229W)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1(YML007W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL183C(YDL183C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR509W(YDR509W_d)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YER053C-A(YER053C-A_p)|FD-Score:5.78|P-value:3.81E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YFR032C-B(YFR032C-B_p)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL088W(YGL088W_d)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIL055C(YIL055C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YJL016W(YJL016W_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YJL169W(YJL169W_d)|FD-Score:-5.23|P-value:8.31E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 Gene:YLL047W(YLL047W_d)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YMR052C-A(YMR052C-A_d)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR074C(YMR074C)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR262W(YMR262W_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YOR1(YGR281W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:ZUO1(YGR285C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W13.101.61E-395.38LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W7.725.87E-152.18NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YKR068C5.541.50E-80.63BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YCL052C4.924.42E-70.49PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YDR188W4.424.91E-60.36CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YJL014W4.062.43E-50.14CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL091C3.934.29E-50.19GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YDL015C3.749.26E-50.10TSC13Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
YNL131W3.641.38E-47.96E-4TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YGR094W3.641.38E-40.14VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase
YKL095W3.492.40E-40.10YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YHR024C3.403.43E-40.02MAS2Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YPR016C3.383.69E-40.00TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YAL041W3.373.74E-40.13CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDL209C3.255.87E-40.02CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL202W6.504.04E-11MRPL11Mitochondrial ribosomal protein of the large subunit
YER053C-A_p5.783.81E-9YER053C-A_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YLL018C-A5.393.53E-8COX19Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
YMR271C5.364.17E-8URA10Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
YMR052C-A_d5.171.15E-7YMR052C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR198C5.141.41E-7BFR1Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
YKL068W5.062.14E-7NUP100FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p
YER039C4.769.84E-7HVG1Protein of unknown function, has homology to Vrg4p
YPR062W4.721.19E-6FCY1Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
YBL087C4.711.23E-6RPL23ARibosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
YMR251W-A4.631.80E-6HOR7Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
YLR131C4.621.95E-6ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YGR148C4.493.59E-6RPL24BRibosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
YLL047W_d4.454.20E-6YLL047W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
YOR229W4.434.71E-6WTM2Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats

GO enrichment analysis for SGTC_579
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2663.36E-96SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.095238160S ribosome export
0.2174.51E-64SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.12560S ribosome export
0.2076.34E-58SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.13559360S ribosome export
0.2068.70E-58SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.11111160S ribosome export
0.1924.71E-50SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.11764760S ribosome export
0.1817.58E-45SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.17187560S ribosome export
0.1777.47E-43SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.0560S ribosome export
0.1694.10E-39SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.11111160S ribosome export
0.1612.13E-35SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.23076960S ribosome export
0.1602.47E-35SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.18055660S ribosome export
0.1512.28E-31SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.13432860S ribosome export
0.1476.33E-30SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.112560S ribosome export
0.1479.57E-30SGTC_9761348-1067 47.6 μMChemDiv (Drug-like library)53106710.18333360S ribosome export
0.1454.16E-29SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.15151560S ribosome export
0.1455.67E-29SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.14492860S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_15014491-027538.4 μM0.450985104718ChemDiv (Drug-like library)315.388365.01622
SGTC_13261431-209410.8 μM0.4616278ChemDiv (Drug-like library)234.295764.59112tubulin folding & SWR complex
SGTC_13601598-002239.8 μM0.375761525ChemDiv (Drug-like library)248.322345.07712ERG2
SGTC_23549060492162 μM0.3469396471895Chembridge (Fragment library)201.26761.27823
SGTC_730327-032516.23 μM0.33333353298ChemDiv (Drug-like library)284.354445.38912DNA damage response
SGTC_13833049-003220.7 μM0.320755712864ChemDiv (Drug-like library)291.347084.0261460S ribosome export
SGTC_6074491-098666.8 μM0.314815698981ChemDiv (Drug-like library)296.793985.48512heme requiring
SGTC_10044084-0029199 μM0.303571716894ChemDiv (Drug-like library)323.412083.22414
SGTC_6450845-09435.41 μM0.2857141885106ChemDiv (Drug-like library)425.307226.7211460S ribosome export
SGTC_11891431-21197.28 μM0.2857143107275ChemDiv (Drug-like library)254.714244.76912