1488-1091

1-(4-methylphenyl)-1-(4-methylphenyl)sulfonyl-3-[(4-methylphenyl)sulfonylcarbamoyl]urea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_583
Screen concentration 88.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 4303273
SMILES CC1=CC=C(C=C1)N(C(=O)NC(=O)NS(=O)(=O)C2=CC=C(C=C2)C)S(=O)(=O)C3=CC=C(C=C3)C
Standardized SMILES Cc1ccc(cc1)N(C(=O)NC(=O)NS(=O)(=O)c2ccc(C)cc2)S(=O)(=O)c3ccc(C)cc3
Molecular weight 501.5752
ALogP 4.69
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 41.75
% growth inhibition (Hom. pool) 11.5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4303273
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:4.29|P-value:8.89E-6|Clearance:0.42||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BRN1(YBL097W)|FD-Score:3.25|P-value:5.79E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DRE2(YKR071C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.06||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:GLC7(YER133W)|FD-Score:8.78|P-value:8.52E-19|Clearance:2.34||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRR1(YIL026C)|FD-Score:3.21|P-value:6.73E-4|Clearance:0.04||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:PRP9(YDL030W)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.11||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RPB5(YBR154C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.1||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL10(YLR075W)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL15A(YLR029C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.44|P-value:6.16E-11|Clearance:0.45||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.03||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:4.57|P-value:2.45E-6|Clearance:0.28||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SUI2(YJR007W)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.01||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TUB1(YML085C)|FD-Score:5.98|P-value:1.09E-9|Clearance:1.41||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YLR076C(YLR076C_d)|FD-Score:3.87|P-value:5.35E-5|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ACT1(YFL039C)|FD-Score:4.29|P-value:8.89E-6|Clearance:0.42||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BRN1(YBL097W)|FD-Score:3.25|P-value:5.79E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DRE2(YKR071C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.06||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:GLC7(YER133W)|FD-Score:8.78|P-value:8.52E-19|Clearance:2.34||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRR1(YIL026C)|FD-Score:3.21|P-value:6.73E-4|Clearance:0.04||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:PRP9(YDL030W)|FD-Score:3.46|P-value:2.70E-4|Clearance:0.11||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RPB5(YBR154C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.1||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL10(YLR075W)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.04||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL15A(YLR029C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.44|P-value:6.16E-11|Clearance:0.45||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS15(YOL040C)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RPS2(YGL123W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.03||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:4.57|P-value:2.45E-6|Clearance:0.28||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:3.16|P-value:7.76E-4|Clearance:0.01||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SUI2(YJR007W)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.01||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TUB1(YML085C)|FD-Score:5.98|P-value:1.09E-9|Clearance:1.41||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YLR076C(YLR076C_d)|FD-Score:3.87|P-value:5.35E-5|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4303273
Download HOP data (tab-delimited text)  (excel)
Gene:ARO1(YDR127W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:9.11|P-value:3.98E-20||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASR1(YPR093C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG26(YLR189C)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BUL2(YML111W)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CAP1(YKL007W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CEM1(YER061C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:COX19(YLL018C-A)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CTR1(YPR124W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL2(YIR029W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMA1(YHR115C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:EFM2(YBR271W)|FD-Score:4.36|P-value:6.42E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:EMI1(YDR512C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ETT1(YOR051C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:EXO5(YBR163W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:GCN20(YFR009W)|FD-Score:7.74|P-value:5.16E-15||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.07|P-value:6.27E-10||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLK1(YCL040W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GPD2(YOL059W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HNT3(YOR258W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IOC4(YMR044W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC5(YFR038W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:KES1(YPL145C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:4.82|P-value:7.12E-7||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MTC7(YEL033W_p)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:PAC10(YGR078C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PPN1(YDR452W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:RAD61(YDR014W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RGC1(YPR115W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RNQ1(YCL028W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:ROX1(YPR065W)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RRG1(YDR065W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SGO1(YOR073W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SLX4(YLR135W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SNA2(YDR525W-A)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNC2(YOR327C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SPG1(YGR236C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SRO9(YCL037C)|FD-Score:6.03|P-value:8.04E-10||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSA3(YBL075C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:THP2(YHR167W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TPN1(YGL186C)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:5.63|P-value:9.19E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.85|P-value:2.43E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.78|P-value:8.93E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:URC2(YDR520C_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPS68(YOL129W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VTS1(YOR359W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YAR023C(YAR023C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YCR099C(YCR099C_p)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Putative protein of unknown function Gene:YDR008C(YDR008C_d)|FD-Score:6.12|P-value:4.67E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER034W(YER034W_p)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL117W(YGL117W_p)|FD-Score:7.16|P-value:4.07E-13||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:4.49|P-value:3.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YLR202C(YLR202C_d)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YPL068C(YPL068C_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR063C(YPR063C_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:ER-localized protein of unknown function Gene:ARO1(YDR127W)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:9.11|P-value:3.98E-20||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASR1(YPR093C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG26(YLR189C)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy Gene:BUL2(YML111W)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CAP1(YKL007W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CEM1(YER061C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:COX19(YLL018C-A)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CTR1(YPR124W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAL2(YIR029W)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMA1(YHR115C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:EFM2(YBR271W)|FD-Score:4.36|P-value:6.42E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:EMI1(YDR512C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ETT1(YOR051C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:EXO5(YBR163W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:GCN20(YFR009W)|FD-Score:7.74|P-value:5.16E-15||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.07|P-value:6.27E-10||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLK1(YCL040W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GPD2(YOL059W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HNT3(YOR258W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IOC4(YMR044W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:IRC5(YFR038W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:KES1(YPL145C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAG2(YOL025W)|FD-Score:4.82|P-value:7.12E-7||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MTC7(YEL033W_p)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:PAC10(YGR078C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PPN1(YDR452W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:RAD61(YDR014W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin Gene:RGC1(YPR115W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RNQ1(YCL028W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:ROX1(YPR065W)|FD-Score:3.77|P-value:8.01E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RRG1(YDR065W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SGO1(YOR073W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SLX4(YLR135W)|FD-Score:-4.13|P-value:1.81E-5||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SNA2(YDR525W-A)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNC2(YOR327C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SPG1(YGR236C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SRO9(YCL037C)|FD-Score:6.03|P-value:8.04E-10||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSA3(YBL075C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:THP2(YHR167W)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TPN1(YGL186C)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:5.63|P-value:9.19E-9||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.85|P-value:2.43E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.78|P-value:8.93E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:URC2(YDR520C_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VPS68(YOL129W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VTS1(YOR359W)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YAR023C(YAR023C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YCR099C(YCR099C_p)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Putative protein of unknown function Gene:YDR008C(YDR008C_d)|FD-Score:6.12|P-value:4.67E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER034W(YER034W_p)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YGL117W(YGL117W_p)|FD-Score:7.16|P-value:4.07E-13||SGD DESC:Putative protein of unknown function Gene:YGR290W(YGR290W_d)|FD-Score:4.49|P-value:3.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YIR020W-A(YIR020W-A_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YLR202C(YLR202C_d)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YPL068C(YPL068C_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR063C(YPR063C_p)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:ER-localized protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W8.788.52E-192.34GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YHR062C6.446.16E-110.45RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YML085C5.981.09E-91.41TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YHL015W4.572.45E-60.28RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YFL039C4.298.89E-60.42ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLR076C_d3.875.35E-50.28YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YKR071C3.601.61E-40.06DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YOL040C3.542.00E-40.08RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YDL030W3.462.70E-40.11PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YBR154C3.353.98E-40.10RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YJR007W3.265.61E-40.01SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YBL097W3.255.79E-40.00BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YLR075W3.255.83E-40.04RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YIL026C3.216.73E-40.04IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YNL178W3.167.76E-40.01RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR035W9.113.98E-20ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YFR009W7.745.16E-15GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YGL117W_p7.164.07E-13YGL117W_pPutative protein of unknown function
YDR008C_d6.124.67E-10YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR026C6.076.27E-10GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YCL037C6.038.04E-10SRO9Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
YKL211C5.852.43E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR007W5.639.19E-9TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YOL025W4.827.12E-7LAG2Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1
YDR354W4.788.93E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGR290W_d4.493.65E-6YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YBR271W4.366.42E-6EFM2S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis
YIR029W4.241.13E-5DAL2Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YOR327C4.201.36E-5SNC2Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication
YDR014W4.102.06E-5RAD61Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin

GO enrichment analysis for SGTC_583
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3992.85E-224SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.555556
0.3892.01E-212SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.588235
0.3736.16E-194SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0571429
0.3714.46E-192SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0571429
0.3472.35E-166SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0769231
0.3435.49E-163SGTC_18825615643 20.0 μMMiscellaneous22530750.0886076TRP & mitochondrial translation
0.3421.24E-161SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0597015
0.3384.01E-158SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0886076
0.3359.92E-155SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.084507
0.3223.70E-142SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0740741
0.3192.05E-139SGTC_24885268135 45.7 μMMiscellaneous12703560.0625
0.3154.86E-136SGTC_2851486-1325 46.0 μMChemDiv (Drug-like library)223102300.638298
0.3155.74E-136SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0833333
0.3141.72E-135SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.108108
0.3131.09E-134SGTC_1761486-1328 72.8 μMChemDiv (Drug-like library)223102310.508475

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2851486-132546 μM0.63829822310230ChemDiv (Drug-like library)457.92995.29624
SGTC_13391486-132122.7 μM0.63469382ChemDiv (Drug-like library)443.903324.8124ERG2
SGTC_7131488-1073324 μM0.5918373312542ChemDiv (Drug-like library)493.596284.48926ERG2
SGTC_2051486-133071.9 μM0.5882354118834ChemDiv (Drug-like library)485.983066.26924
SGTC_1941486-132356.9 μM0.5769235178754ChemDiv (Drug-like library)478.348385.47524
SGTC_13371486-101140.3 μM0.568627X1337ChemDiv (Drug-like library)443.903314.8142
SGTC_1801486-1293102.78 μM0.5555564140536ChemDiv (Drug-like library)491.482815.57427
SGTC_9811488-109454.9 μM0.5263163449522ChemDiv (Drug-like library)489.564523.20327
SGTC_11901486-133213 μM0.5263164150046ChemDiv (Drug-like library)487.955885.14325
SGTC_1761486-132872.83 μM0.50847522310231ChemDiv (Drug-like library)511.901295.75327