3909-8003

4-pyrrolidin-1-yl-5,6,7,8-tetrahydro-[1]benzothiolo[2,3-d]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_595
Screen concentration 73.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 699517
SMILES C1CCC2=C(C1)C3=C(N=CN=C3S2)N4CCCC4
Standardized SMILES C1CCN(C1)c2ncnc3sc4CCCCc4c23
Molecular weight 259.3699
ALogP 3.66
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.81
% growth inhibition (Hom. pool) 11.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 699517
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.22||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ACT1(YFL039C)|FD-Score:3.22|P-value:6.52E-4|Clearance:0.1||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:LSM2(YBL026W)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NAR1(YNL240C)|FD-Score:5.05|P-value:2.21E-7|Clearance:0.54||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:ORC1(YML065W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.08||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:RHO1(YPR165W)|FD-Score:-3.35|P-value:3.97E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SEC13(YLR208W)|FD-Score:6.96|P-value:1.72E-12|Clearance:1.91||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:4.5|P-value:3.32E-6|Clearance:0.22||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TAF10(YDR167W)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.04||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TIP20(YGL145W)|FD-Score:4.28|P-value:9.33E-6|Clearance:0.72||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:YBL073W(YBL073W_d)|FD-Score:3.34|P-value:4.20E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YDL016C(YDL016C_d)|FD-Score:-3.95|P-value:3.87E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YGR114C(YGR114C_d)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ACC1(YNR016C)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.22||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ACT1(YFL039C)|FD-Score:3.22|P-value:6.52E-4|Clearance:0.1||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:LSM2(YBL026W)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.01||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NAR1(YNL240C)|FD-Score:5.05|P-value:2.21E-7|Clearance:0.54||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:ORC1(YML065W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.08||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:RHO1(YPR165W)|FD-Score:-3.35|P-value:3.97E-4|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:SEC13(YLR208W)|FD-Score:6.96|P-value:1.72E-12|Clearance:1.91||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:4.5|P-value:3.32E-6|Clearance:0.22||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TAF10(YDR167W)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.04||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TIP20(YGL145W)|FD-Score:4.28|P-value:9.33E-6|Clearance:0.72||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:YBL073W(YBL073W_d)|FD-Score:3.34|P-value:4.20E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YDL016C(YDL016C_d)|FD-Score:-3.95|P-value:3.87E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YGR114C(YGR114C_d)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 699517
Download HOP data (tab-delimited text)  (excel)
Gene:BIO3(YNR058W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BUD30(YDL151C_d)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCW12(YLR110C)|FD-Score:4.8|P-value:7.76E-7||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHO1(YER026C)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CMR3(YPR013C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CRP1(YHR146W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTF8(YHR191C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DBF2(YGR092W)|FD-Score:8.43|P-value:1.71E-17||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:EFG1(YGR271C-A)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERP2(YAL007C)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:GAP1(YKR039W)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GTO1(YGR154C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HXT1(YHR094C)|FD-Score:4|P-value:3.15E-5||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:LTV1(YKL143W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MMP1(YLL061W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MOH1(YBL049W)|FD-Score:4.76|P-value:9.75E-7||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPL25(YGR076C)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:NPL3(YDR432W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OPI9(YLR338W_d)|FD-Score:5.67|P-value:7.21E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:QCR2(YPR191W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RPL22A(YLR061W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:SCO1(YBR037C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SIA1(YOR137C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIP3(YNL257C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SKI8(YGL213C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SKT5(YBL061C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI2(YBR240C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRP3(YKL211C)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBR2(YLR024C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VBA3(YCL069W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA3(YEL027W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:YDR541C(YDR541C_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER039C-A(YER039C-A_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YGR064W(YGR064W_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR228W(YGR228W_d)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHP1(YDR451C)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YLR111W(YLR111W_d)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL014W(YPL014W_p)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:ZEO1(YOL109W)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:BIO3(YNR058W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BUD30(YDL151C_d)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCW12(YLR110C)|FD-Score:4.8|P-value:7.76E-7||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHO1(YER026C)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CMR3(YPR013C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative zinc finger protein; YPR013C is not an essential gene Gene:CRP1(YHR146W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTF8(YHR191C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DBF2(YGR092W)|FD-Score:8.43|P-value:1.71E-17||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:EFG1(YGR271C-A)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERP2(YAL007C)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:GAP1(YKR039W)|FD-Score:3.31|P-value:4.75E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GTO1(YGR154C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HXT1(YHR094C)|FD-Score:4|P-value:3.15E-5||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:LTV1(YKL143W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MMP1(YLL061W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MOH1(YBL049W)|FD-Score:4.76|P-value:9.75E-7||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPL25(YGR076C)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:NPL3(YDR432W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OPI9(YLR338W_d)|FD-Score:5.67|P-value:7.21E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:QCR2(YPR191W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Subunit 2 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme Gene:RPL22A(YLR061W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPL8B(YLL045C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:SCO1(YBR037C)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication Gene:SIA1(YOR137C)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIP3(YNL257C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SKI8(YGL213C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SKT5(YBL061C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THI2(YBR240C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRP3(YKL211C)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBR2(YLR024C)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VBA3(YCL069W)|FD-Score:3.8|P-value:7.17E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA3(YEL027W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:YDR541C(YDR541C_p)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER039C-A(YER039C-A_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YGR064W(YGR064W_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR228W(YGR228W_d)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHP1(YDR451C)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YLR111W(YLR111W_d)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL014W(YPL014W_p)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:ZEO1(YOL109W)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W6.961.72E-121.91SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YNL240C5.052.21E-70.55NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YDL195W4.503.32E-60.22SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YGL145W4.289.33E-60.72TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YNR016C3.561.86E-40.22ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YBL073W_d3.344.20E-41.89E-5YBL073W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C
YML065W3.344.20E-40.08ORC1Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
YBL026W3.265.55E-40.01LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDR167W3.255.77E-40.04TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YFL039C3.226.52E-40.10ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YGR114C_d3.119.24E-40.05YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR117C3.070.001080.01CLF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
YAL025C3.060.001120.05MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
YLR291C3.010.001320.01GCD7Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YER006W3.000.001350.07NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR092W8.431.71E-17DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YLR338W_d5.677.21E-9OPI9_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
YLR110C4.807.76E-7CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YBL049W4.769.75E-7MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YGR228W_d4.751.01E-6YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
YPL014W_p4.691.35E-6YPL014W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
YKL211C4.611.98E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGR076C4.395.78E-6MRPL25Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
YGR271C-A4.141.76E-5EFG1Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
YLR111W_d4.022.88E-5YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHR094C4.003.15E-5HXT1Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication
YCL069W3.807.17E-5VBA3Permease of basic amino acids in the vacuolar membrane
YOL109W3.787.76E-5ZEO1Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria
YJL004C3.749.22E-5SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YKL143W3.739.61E-5LTV1Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature

GO enrichment analysis for SGTC_595
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2493.51E-84SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.03125
0.2138.90E-62SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0307692
0.2091.43E-59SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0895522
0.1802.70E-44SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.0298507
0.1796.60E-44SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.107692
0.1718.19E-40SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.0517241
0.1668.91E-38SGTC_1614065-0411 162.1 μMChemDiv (Drug-like library)19062810.0769231
0.1611.09E-35SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.03125
0.1598.57E-35SGTC_9933909-8757 62.7 μMChemDiv (Drug-like library)42883240.166667
0.1561.88E-33SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0625
0.1461.38E-29SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.0952381RPP1 & pyrimidine depletion
0.1441.04E-28SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.0483871
0.1381.29E-26SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.0307692
0.1352.29E-25SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.0307692
0.1352.28E-25SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.028169

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8720588-0139234 μM0.4791672047100ChemDiv (Drug-like library)289.395862.8405
SGTC_1472k841-0093193 μM0.433962X1472ChemDiv (Drug-like library)345.459113.21750
SGTC_5294076-030230.3 μM0.389835816522ChemDiv (Drug-like library)324.400144.41126mitochondrial processes
SGTC_8010866-002810.1 μM0.3709686849771ChemDiv (Drug-like library)347.436783.65326
SGTC_8184072-270753.5 μM0.369565882871ChemDiv (Drug-like library)272.40844.35313
SGTC_24036032726181.2 μM0.342881040Miscellaneous265.39763.80615
SGTC_22587960471200 μM0.3269232974708Chembridge (Fragment library)298.834762.64715
SGTC_3327914064826.93 μM0.3214298029710Chembridge (Drug-like library)320.47613.9116
SGTC_2396534295042.78 μM0.3090911886677Miscellaneous277.40833.96715RSC complex & mRNA processing
SGTC_7093807-1509243 μM0.3090911886677ChemDiv (Drug-like library)277.40833.96715RSC complex & mRNA processing