1155-0152

2-methyl-5-nitro-1H-indole

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_596
Screen concentration 116.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 280313
SMILES CC1=CC2=C(N1)C=CC(=C2)[N+](=O)[O-]
Standardized SMILES Cc1cc2cc(ccc2[nH]1)[N+](=O)[O-]
Molecular weight 176.172
ALogP 2.16
H-bond donor count 1
H-bond acceptor count 2
Response signature TRP & mitochondrial translation

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.24
% growth inhibition (Hom. pool) 12.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 280313
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.87|P-value:5.50E-5|Clearance:0.01||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:DAD2(YKR083C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.08||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DRS1(YLL008W)|FD-Score:3.77|P-value:8.11E-5|Clearance:0.09||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:IRR1(YIL026C)|FD-Score:5.9|P-value:1.85E-9|Clearance:0.83||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LIP1(YMR298W)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.08||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSM4(YER112W)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAK16(YAL025C)|FD-Score:3.18|P-value:7.32E-4|Clearance:0.1||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:NUS1(YDL193W)|FD-Score:4.29|P-value:9.03E-6|Clearance:0.39||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE8(YML092C)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.03||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RER2(YBR002C)|FD-Score:6.75|P-value:7.57E-12|Clearance:0.85||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIX1(YHR197W)|FD-Score:3.86|P-value:5.65E-5|Clearance:0.09||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPL17A(YKL180W)|FD-Score:4.63|P-value:1.82E-6|Clearance:0.34||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN7(YPR108W)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPP1(YHR062C)|FD-Score:-4.89|P-value:5.12E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:5.07|P-value:2.00E-7|Clearance:0.44||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEC20(YDR498C)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.22||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SPP41(YDR464W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.08||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:ARP2(YDL029W)|FD-Score:3.87|P-value:5.50E-5|Clearance:0.01||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:DAD2(YKR083C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.08||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DRS1(YLL008W)|FD-Score:3.77|P-value:8.11E-5|Clearance:0.09||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:IRR1(YIL026C)|FD-Score:5.9|P-value:1.85E-9|Clearance:0.83||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:LIP1(YMR298W)|FD-Score:3.26|P-value:5.63E-4|Clearance:0.08||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSM4(YER112W)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAK16(YAL025C)|FD-Score:3.18|P-value:7.32E-4|Clearance:0.1||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:NUS1(YDL193W)|FD-Score:4.29|P-value:9.03E-6|Clearance:0.39||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE8(YML092C)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.03||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RER2(YBR002C)|FD-Score:6.75|P-value:7.57E-12|Clearance:0.85||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIX1(YHR197W)|FD-Score:3.86|P-value:5.65E-5|Clearance:0.09||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPL17A(YKL180W)|FD-Score:4.63|P-value:1.82E-6|Clearance:0.34||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPN7(YPR108W)|FD-Score:3.31|P-value:4.69E-4|Clearance:0.05||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPP1(YHR062C)|FD-Score:-4.89|P-value:5.12E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:5.07|P-value:2.00E-7|Clearance:0.44||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEC20(YDR498C)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.22||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SPP41(YDR464W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.08||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 280313
Download HOP data (tab-delimited text)  (excel)
Gene:ABF2(YMR072W)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ACO1(YLR304C)|FD-Score:6.19|P-value:3.05E-10||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AEP2(YMR282C)|FD-Score:4.77|P-value:9.21E-7||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:APL2(YKL135C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:12.9|P-value:3.68E-38||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:6.84|P-value:4.02E-12||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATP14(YLR295C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP15(YPL271W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP3(YBR039W)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CAC2(YML102W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CAT2(YML042W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CPR7(YJR032W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CUR1(YPR158W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA4(YHR011W)|FD-Score:5.48|P-value:2.11E-8||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EFG1(YGR271C-A)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FAB1(YFR019W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:GCN2(YDR283C)|FD-Score:9.78|P-value:7.05E-23||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.47|P-value:4.99E-11||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HBT1(YDL223C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HMO1(YDR174W)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSP31(YDR533C)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HUG1(YML058W-A)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IMG2(YCR071C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC15(YPL017C)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:KEL1(YHR158C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:MBP1(YDL056W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MGM101(YJR144W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MHR1(YDR296W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRF1(YGL143C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRH4(YGL064C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRM1(YOR201C)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRPL17(YNL252C)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL24(YMR193W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL27(YBR282W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL28(YDR462W)|FD-Score:4.92|P-value:4.36E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL37(YBR268W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPL8(YJL063C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS28(YDR337W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS35(YGR165W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:5.33|P-value:4.93E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSM1(YGR171C)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MTF1(YMR228W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:MTG1(YMR097C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NUM1(YDR150W)|FD-Score:4.95|P-value:3.81E-7||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCA5(YHL029C_p)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI9(YLR338W_d)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAP2(YOL115W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PIF1(YML061C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PMD1(YER132C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PRR2(YDL214C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:REG1(YDR028C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RML2(YEL050C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL16B(YNL069C)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM19(YNR037C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM23(YGL129C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RSM27(YGR215W)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:5.62|P-value:9.30E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SDL1(YIL167W)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SFP1(YLR403W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SHE4(YOR035C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SSE1(YPL106C)|FD-Score:5.52|P-value:1.66E-8||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SUV3(YPL029W)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAL1(YLR354C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TOM1(YDR457W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRP1(YDR007W)|FD-Score:7.34|P-value:1.07E-13||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.67|P-value:2.25E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:URC2(YDR520C_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAC8(YEL013W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS28(YPL065W)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YBR134W(YBR134W_d)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDL144C(YDL144C_p)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:6.48|P-value:4.56E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR426C(YDR426C_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YGL117W(YGL117W_p)|FD-Score:4.49|P-value:3.53E-6||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YIL060W(YIL060W_p)|FD-Score:4.34|P-value:7.28E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YKL068W-A(YKL068W-A_p)|FD-Score:6.3|P-value:1.50E-10||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR365W(YLR365W_d)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ABF2(YMR072W)|FD-Score:4.55|P-value:2.73E-6||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ACO1(YLR304C)|FD-Score:6.19|P-value:3.05E-10||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AEP2(YMR282C)|FD-Score:4.77|P-value:9.21E-7||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:APL2(YKL135C)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:12.9|P-value:3.68E-38||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:6.84|P-value:4.02E-12||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATP14(YLR295C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP15(YPL271W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP3(YBR039W)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:CAC2(YML102W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:CAT2(YML042W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CPR7(YJR032W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CUR1(YPR158W)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:4.22|P-value:1.25E-5||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA4(YHR011W)|FD-Score:5.48|P-value:2.11E-8||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EFG1(YGR271C-A)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FAB1(YFR019W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:GCN2(YDR283C)|FD-Score:9.78|P-value:7.05E-23||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.47|P-value:4.99E-11||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HBT1(YDL223C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HMO1(YDR174W)|FD-Score:4.38|P-value:5.86E-6||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSP31(YDR533C)|FD-Score:5.1|P-value:1.72E-7||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HUG1(YML058W-A)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:IMG2(YCR071C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRC15(YPL017C)|FD-Score:-3.8|P-value:7.31E-5||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:KEL1(YHR158C)|FD-Score:4.06|P-value:2.48E-5||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:MBP1(YDL056W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MGM101(YJR144W)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MHR1(YDR296W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRF1(YGL143C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRH4(YGL064C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRM1(YOR201C)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRPL17(YNL252C)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL24(YMR193W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL27(YBR282W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL28(YDR462W)|FD-Score:4.92|P-value:4.36E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL37(YBR268W)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:3.81|P-value:6.82E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPL8(YJL063C)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS28(YDR337W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS35(YGR165W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:5.33|P-value:4.93E-8||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSM1(YGR171C)|FD-Score:4.46|P-value:4.09E-6||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:MTF1(YMR228W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:MTG1(YMR097C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NUM1(YDR150W)|FD-Score:4.95|P-value:3.81E-7||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCA5(YHL029C_p)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI9(YLR338W_d)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAP2(YOL115W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PIF1(YML061C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PMD1(YER132C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PRR2(YDL214C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:REG1(YDR028C)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RML2(YEL050C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL16B(YNL069C)|FD-Score:3.79|P-value:7.39E-5||SGD DESC:Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM19(YNR037C)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RSM23(YGL129C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:RSM27(YGR215W)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:5.62|P-value:9.30E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SDL1(YIL167W)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SFP1(YLR403W)|FD-Score:5.03|P-value:2.44E-7||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SHE4(YOR035C)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SSE1(YPL106C)|FD-Score:5.52|P-value:1.66E-8||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SUV3(YPL029W)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAL1(YLR354C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:TOM1(YDR457W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRP1(YDR007W)|FD-Score:7.34|P-value:1.07E-13||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.67|P-value:2.25E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:URC2(YDR520C_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism Gene:VAC8(YEL013W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPS28(YPL065W)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:YBR134W(YBR134W_d)|FD-Score:4.52|P-value:3.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDL144C(YDL144C_p)|FD-Score:-3.16|P-value:7.94E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:6.48|P-value:4.56E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR426C(YDR426C_d)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YGL117W(YGL117W_p)|FD-Score:4.49|P-value:3.53E-6||SGD DESC:Putative protein of unknown function Gene:YGR219W(YGR219W_d)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YIL060W(YIL060W_p)|FD-Score:4.34|P-value:7.28E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YKL068W-A(YKL068W-A_p)|FD-Score:6.3|P-value:1.50E-10||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR365W(YLR365W_d)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C6.757.57E-120.85RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YIL026C5.901.85E-90.83IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YGL123W5.072.00E-70.44RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YKL180W4.631.82E-60.34RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YDL193W4.299.03E-60.39NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YML092C3.904.79E-50.03PRE8Alpha 2 subunit of the 20S proteasome
YDL029W3.875.50E-50.01ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YHR197W3.865.65E-50.09RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YLL008W3.778.11E-50.09DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YDR498C3.681.17E-40.22SEC20Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
YKR083C3.462.68E-40.08DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YDR464W3.393.55E-40.08SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YPR108W3.314.69E-40.05RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YMR298W3.265.63E-40.08LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YAL025C3.187.32E-40.10MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR127W12.903.68E-38ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR283C9.787.05E-23GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR354W8.672.25E-18TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR007W7.341.07E-13TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR035W6.844.02E-12ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YDR008C_d6.484.56E-11YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR026C6.474.99E-11GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YKL068W-A_p6.301.50E-10YKL068W-A_pPutative protein of unknown function; identified by homology to Ashbya gossypii
YLR304C6.193.05E-10ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YJR113C5.629.30E-9RSM7Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein
YPL106C5.521.66E-8SSE1ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
YHR011W5.482.11E-8DIA4Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
YBR251W5.334.93E-8MRPS5Mitochondrial ribosomal protein of the small subunit
YDR533C5.101.72E-7HSP31Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
YLR403W5.032.44E-7SFP1Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion

GO enrichment analysis for SGTC_596
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4780SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0816327TRP & mitochondrial translation
0.4553.72E-299SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0769231TRP & mitochondrial translation
0.4405.64E-278SGTC_18825615643 20.0 μMMiscellaneous22530750.0985916TRP & mitochondrial translation
0.4343.07E-270SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.0816327TRP & mitochondrial translation
0.4202.32E-251SGTC_24545-benzylidenerhodanine 10.4 μMMiscellaneous12732100.0769231TRP & mitochondrial translation
0.4186.09E-249SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0645161
0.4131.10E-241SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.0652174TRP & mitochondrial translation
0.3852.25E-208SGTC_7670929-0023 86.0 μMChemDiv (Drug-like library)12045630.0833333TRP & mitochondrial translation
0.3834.52E-205SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0365854
0.3496.42E-169SGTC_18815652484 16.0 μMMiscellaneous22551290.1TRP & mitochondrial translation
0.3491.09E-168SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0684932
0.3426.01E-162SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0892857
0.3384.89E-158SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.101449
0.3358.30E-155SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.107143
0.3347.10E-154SGTC_24885268135 45.7 μMMiscellaneous12703560.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1330724-0033378.92 μM0.578947280309ChemDiv (Drug-like library)238.24143.82712
SGTC_490717-096532.13 μM0.3725495889349ChemDiv (Drug-like library)279.293324.0791360S ribosome export
SGTC_3710195-00067.36 μM0.3589743928593ChemDiv (Drug-like library)253.209682.70204
SGTC_1360097-0015404.48 μM0.3333334861337ChemDiv (Drug-like library)494.448642.62701160S ribosome export
SGTC_12590671-013243 μM0.326531X1259ChemDiv (Drug-like library)371.206443.44641
SGTC_7930488-0545106 μM0.325581292548ChemDiv (Drug-like library)237.212042.24105
SGTC_2840988-010432.6 μM0.324666532ChemDiv (Drug-like library)329.3525.28314heme requiring
SGTC_13031160-0052261 μM0.323562476ChemDiv (Drug-like library)281.266142.87413
SGTC_14284049-020461.7 μM0.32X1428ChemDiv (Drug-like library)324.327423.38941
SGTC_1000086-012849.59 μM0.31914993297ChemDiv (Drug-like library)248.234682.08504copper-dependent oxidative stress