k018-0003

3-[(5-nitro-6-oxocyclohexa-2,4-dien-1-ylidene)methylamino]-5,6,7,8-tetrahydro-[1]benzothiolo[2,3-d]pyrimidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_597
Screen concentration 82.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 6786234
SMILES C1CCC2=C(C1)C3=C(S2)N=CN(C3=O)NC=C4C=CC=C(C4=O)[N+](=O)[O-]
Standardized SMILES Oc1c(C=NN2C=Nc3sc4CCCCc4c3C2=O)cccc1[N+](=O)[O-]
Molecular weight 370.3825
ALogP 1.84
H-bond donor count 1
H-bond acceptor count 7
Response signature RNA pol III & RNase P/MRP

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.15
% growth inhibition (Hom. pool) 9.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6786234
Download HIP data (tab-delimited text)  (excel)
Gene:CDC16(YKL022C)|FD-Score:3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:FOL3(YMR113W)|FD-Score:3.79|P-value:7.55E-5|Clearance:0.31||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HSP10(YOR020C)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.01||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LCB2(YDR062W)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.16||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MSL5(YLR116W)|FD-Score:-3.19|P-value:7.12E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:RFC2(YJR068W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.05||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPB8(YOR224C)|FD-Score:3.8|P-value:7.13E-5|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC11(YDR045C)|FD-Score:-3.15|P-value:8.26E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPO31(YOR116C)|FD-Score:5.17|P-value:1.15E-7|Clearance:1.08||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:-5.84|P-value:2.68E-9|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC27(YGL137W)|FD-Score:4.99|P-value:3.02E-7|Clearance:1.08||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SED5(YLR026C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.18||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TOP2(YNL088W)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:WRS1(YOL097C)|FD-Score:3.91|P-value:4.63E-5|Clearance:0.11||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:CDC16(YKL022C)|FD-Score:3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:FOL3(YMR113W)|FD-Score:3.79|P-value:7.55E-5|Clearance:0.31||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HSP10(YOR020C)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.01||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:LCB2(YDR062W)|FD-Score:3.24|P-value:5.93E-4|Clearance:0.16||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MSL5(YLR116W)|FD-Score:-3.19|P-value:7.12E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:RFC2(YJR068W)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.05||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPB8(YOR224C)|FD-Score:3.8|P-value:7.13E-5|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC11(YDR045C)|FD-Score:-3.15|P-value:8.26E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPO31(YOR116C)|FD-Score:5.17|P-value:1.15E-7|Clearance:1.08||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:-5.84|P-value:2.68E-9|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC27(YGL137W)|FD-Score:4.99|P-value:3.02E-7|Clearance:1.08||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SED5(YLR026C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.18||SGD DESC:cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins Gene:TOP2(YNL088W)|FD-Score:-3.11|P-value:9.24E-4|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:WRS1(YOL097C)|FD-Score:3.91|P-value:4.63E-5|Clearance:0.11||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6786234
Download HOP data (tab-delimited text)  (excel)
Gene:AIM7(YDR063W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ALK1(YGL021W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATG12(YBR217W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:CAD1(YDR423C)|FD-Score:4.38|P-value:5.87E-6||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CHD1(YER164W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:DAL7(YIR031C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIA3(YDL024C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DSE2(YHR143W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DSF2(YBR007C_p)|FD-Score:5.12|P-value:1.55E-7||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERF2(YLR246W)|FD-Score:4.33|P-value:7.54E-6||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ERV41(YML067C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:GAS4(YOL132W)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GIT1(YCR098C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPM2(YDL021W)|FD-Score:4.58|P-value:2.27E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:IRC8(YJL051W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:MET8(YBR213W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MSH6(YDR097C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:PDX3(YBR035C)|FD-Score:3.34|P-value:4.11E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PHO90(YJL198W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:RAD51(YER095W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAS2(YNL098C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RGD2(YFL047W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RTT10(YPL183C)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC6(YDR129C)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:TAT1(YBR069C)|FD-Score:-3.8|P-value:7.35E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRF5(YNL299W)|FD-Score:-4.77|P-value:9.18E-7||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRM7(YBR061C)|FD-Score:8.51|P-value:8.93E-18||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:VHS1(YDR247W)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:WSC2(YNL283C)|FD-Score:5.28|P-value:6.38E-8||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:XPT1(YJR133W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL070C(YBL070C_d)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YHR050W-A(YHR050W-A_p)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR127W(YHR127W)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YJR115W(YJR115W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YJR146W(YJR146W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YKL030W(YKL030W_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YML094C-A(YML094C-A_d)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR034C(YMR034C_p)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR254C(YMR254C_d)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL024W(YOL024W_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:AIM7(YDR063W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ALK1(YGL021W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:ATG12(YBR217W)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:CAD1(YDR423C)|FD-Score:4.38|P-value:5.87E-6||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CHD1(YER164W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:DAL7(YIR031C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DIA3(YDL024C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DSE2(YHR143W)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DSF2(YBR007C_p)|FD-Score:5.12|P-value:1.55E-7||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERF2(YLR246W)|FD-Score:4.33|P-value:7.54E-6||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:ERV41(YML067C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:GAS4(YOL132W)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GIT1(YCR098C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPM2(YDL021W)|FD-Score:4.58|P-value:2.27E-6||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:IRC8(YJL051W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:MET8(YBR213W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Bifunctional dehydrogenase and ferrochelatase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis Gene:MSH6(YDR097C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:PDX3(YBR035C)|FD-Score:3.34|P-value:4.11E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PHO90(YJL198W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:RAD51(YER095W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAS2(YNL098C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RGD2(YFL047W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RTT10(YPL183C)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC6(YDR129C)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:TAT1(YBR069C)|FD-Score:-3.8|P-value:7.35E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRF5(YNL299W)|FD-Score:-4.77|P-value:9.18E-7||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRM7(YBR061C)|FD-Score:8.51|P-value:8.93E-18||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:VHS1(YDR247W)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p Gene:WSC2(YNL283C)|FD-Score:5.28|P-value:6.38E-8||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:XPT1(YJR133W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Xanthine-guanine phosphoribosyl transferase, required for xanthine utilization and for optimal utilization of guanine Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL070C(YBL070C_d)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YHR050W-A(YHR050W-A_p)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function; identified by expression profiling and mass spectrometry Gene:YHR127W(YHR127W)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YJR115W(YJR115W_p)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YJR146W(YJR146W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YKL030W(YKL030W_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YML094C-A(YML094C-A_d)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR034C(YMR034C_p)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR254C(YMR254C_d)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL024W(YOL024W_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR116C5.171.15E-71.08RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YGL137W4.993.02E-71.08SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YOL097C3.914.63E-50.11WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YOR224C3.807.13E-50.01RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YMR113W3.797.55E-50.31FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YJR068W3.482.52E-40.05RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR020C3.433.01E-40.01HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YLR026C3.423.11E-40.18SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YKL022C3.245.92E-47.09E-4CDC16Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation
YDR062W3.245.93E-40.16LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YOR340C3.080.001030.06RPA43RNA polymerase I subunit A43
YDR168W3.020.001280.02CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YJR046W3.000.001340.10TAH11DNA replication licensing factor, required for pre-replication complex assembly
YJR076C2.910.001830.11CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YOR075W2.800.002560.08UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR061C8.518.93E-18TRM72'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively
YNL283C5.286.38E-8WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YBR007C_p5.121.55E-7DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR129C5.081.84E-7SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YDL021W4.582.27E-6GPM2Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event
YDR063W4.483.65E-6AIM7Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
YDR423C4.385.87E-6CAD1AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
YLR246W4.337.54E-6ERF2Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole
YER095W4.181.44E-5RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YPL183C4.171.51E-5RTT10WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
YAL056C-A_d3.993.32E-5YAL056C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER164W3.934.28E-5CHD1Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
YFL047W3.816.92E-5RGD2GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YCR098C3.816.92E-5GIT1Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
YOL132W3.749.03E-5GAS41,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall

GO enrichment analysis for SGTC_597
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1625.48E-36SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.352113RNA pol III & RNase P/MRP
0.1619.91E-36SGTC_712r015-0009 80.6 μMChemDiv (Drug-like library)68138720.191781
0.1612.04E-35SGTC_608k048-0125 159.0 μMChemDiv (Drug-like library)68176520.176471
0.1575.14E-34SGTC_659k018-0002 107.0 μMChemDiv (Drug-like library)67978790.608696
0.1102.00E-17SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.0853659RNA pol III & RNase P/MRP
0.1081.14E-16SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.0970149RNA pol III & RNase P/MRP
0.0945.89E-13SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0657895DNA damage response
0.0912.40E-12SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.0581395RNA pol III & RNase P/MRP
0.0855.06E-11SGTC_23569061756 189.0 μMChembridge (Fragment library)201040430.091954
0.0841.13E-10SGTC_23559061122 103.1 μMChembridge (Fragment library)53029570.0617284
0.0808.20E-10SGTC_14984478-3555 97.6 μMChemDiv (Drug-like library)34379190.114943
0.0791.38E-9SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.116505RNA pol III & RNase P/MRP
0.0723.31E-8SGTC_5914299-0174 154.0 μMChemDiv (Drug-like library)55090880.126437
0.0723.87E-8SGTC_23579067248 200.0 μMChembridge (Fragment library)91502350.101266
0.0699.22E-8SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.101449RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_659k018-0002107 μM0.6086966797879ChemDiv (Drug-like library)392.387981.97217
SGTC_13201313-048195.3 μM0.5416676813365ChemDiv (Drug-like library)355.410881.78216
SGTC_6781218-217985.9 μM0.3521136820081ChemDiv (Drug-like library)272.256081.82515RNA pol III & RNase P/MRP
SGTC_13121218-2183165 μM0.3378386744073ChemDiv (Drug-like library)299.281420.96225
SGTC_9771348-1378135 μM0.325X977ChemDiv (Drug-like library)362.342162.83982
SGTC_23955646831180.19 μM0.2988516808628Miscellaneous401.414664.7516
SGTC_8510394-0702453 μM0.289855762646ChemDiv (Drug-like library)216.235883.40112mitochondrial processes
SGTC_13843089-0840101 μM0.2751069836ChemDiv (Drug-like library)354.449264.5814
SGTC_5294076-030230.3 μM0.265065816522ChemDiv (Drug-like library)324.400144.41126mitochondrial processes
SGTC_2139557869256.47 μM0.257143650991Chembridge (Fragment library)207.248962.1904superoxide