3794-0023

5-[[4-[(4-chlorophenyl)methoxy]phenyl]methylidene]-1,3-diazinane-2,4,6-trione

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_603
Screen concentration 45.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 3802260
SMILES C1=CC(=CC=C1COC2=CC=C(C=C2)C=C3C(=O)NC(=O)NC3=O)Cl
Standardized SMILES Clc1ccc(COc2ccc(C=C3C(=O)NC(=O)NC3=O)cc2)cc1
Molecular weight 356.7598
ALogP 3.1
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.91
% growth inhibition (Hom. pool) 7.76


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3802260
Download HIP data (tab-delimited text)  (excel)
Gene:CDC123(YLR215C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.02||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DOP1(YDR141C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:NAR1(YNL240C)|FD-Score:5.65|P-value:8.23E-9|Clearance:1.84||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:RPO21(YDL140C)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.06||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:SRP72(YPL210C)|FD-Score:3.13|P-value:8.76E-4|Clearance:0.05||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TAF12(YDR145W)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YGR114C(YGR114C_d)|FD-Score:3.81|P-value:6.95E-5|Clearance:0.6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:CDC123(YLR215C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.02||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:DOP1(YDR141C)|FD-Score:-3.68|P-value:1.18E-4|Clearance:0||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:NAR1(YNL240C)|FD-Score:5.65|P-value:8.23E-9|Clearance:1.84||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:RPO21(YDL140C)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.06||SGD DESC:RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime Gene:SRP72(YPL210C)|FD-Score:3.13|P-value:8.76E-4|Clearance:0.05||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TAF12(YDR145W)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YGR114C(YGR114C_d)|FD-Score:3.81|P-value:6.95E-5|Clearance:0.6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3802260
Download HOP data (tab-delimited text)  (excel)
Gene:AIM7(YDR063W)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:5.26|P-value:7.02E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG23(YLR431C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BNI5(YNL166C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:CCW12(YLR110C)|FD-Score:5.7|P-value:6.04E-9||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CRP1(YHR146W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:DAL81(YIR023W)|FD-Score:7.6|P-value:1.45E-14||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA1(YMR316W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:EAF3(YPR023C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:GAL4(YPL248C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GCN2(YDR283C)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HXK2(YGL253W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IZH2(YOL002C)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:LYS12(YIL094C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAK31(YCR020C-A)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MDM30(YLR368W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MGR2(YPL098C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:NTH2(YBR001C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:ODC2(YOR222W)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PSP1(YDR505C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PTP1(YDL230W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REI1(YBR267W)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RTT103(YDR289C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SGF29(YCL010C)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SPC1(YJR010C-A)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.34|P-value:1.15E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA3(YDL170W)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS71(YML041C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCL042W(YCL042W_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:-4.68|P-value:1.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL163C(YIL163C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR146W(YJR146W_p)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YMR034C(YMR034C_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YOR041C(YOR041C_d)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YPR1(YDR368W)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication Gene:AIM7(YDR063W)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:5.26|P-value:7.02E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG23(YLR431C)|FD-Score:-3.96|P-value:3.79E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BNI5(YNL166C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner Gene:CCW12(YLR110C)|FD-Score:5.7|P-value:6.04E-9||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CRP1(YHR146W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:DAL81(YIR023W)|FD-Score:7.6|P-value:1.45E-14||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA1(YMR316W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:EAF3(YPR023C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:GAL4(YPL248C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GCN2(YDR283C)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HXK2(YGL253W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:IZH2(YOL002C)|FD-Score:5.54|P-value:1.50E-8||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:LYS12(YIL094C)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAK31(YCR020C-A)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MDM30(YLR368W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MGR2(YPL098C)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:NTH2(YBR001C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:ODC2(YOR222W)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PSP1(YDR505C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:PTP1(YDL230W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REI1(YBR267W)|FD-Score:-4|P-value:3.15E-5||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RTT103(YDR289C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SGF29(YCL010C)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation Gene:SPC1(YJR010C-A)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.34|P-value:1.15E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UGA3(YDL170W)|FD-Score:3.74|P-value:9.24E-5||SGD DESC:Transcriptional activator necessary for gamma-aminobutyrate (GABA)-dependent induction of GABA genes (such as UGA1, UGA2, UGA4); zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus Gene:VPS71(YML041C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YCL042W(YCL042W_p)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:-4.68|P-value:1.42E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL163C(YIL163C_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJR146W(YJR146W_p)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2 Gene:YMR034C(YMR034C_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YOR041C(YOR041C_d)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YPR1(YDR368W)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL240C5.658.23E-91.84NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YGR114C_d3.816.95E-50.59YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR215C3.216.53E-40.02CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YDL140C3.197.09E-40.06RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
YPL210C3.138.76E-40.05SRP72Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
YNL124W3.080.001020.24NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YPL235W2.840.002240.08RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YGL102C_d2.760.002850.02YGL102C_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
YJL111W2.750.002990.07CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YJL034W2.680.003700.04KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YDL165W2.630.004230.01CDC36Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YBR160W2.620.004390.01CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YDL126C2.610.004540.08CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YML130C2.530.005690.05ERO1Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
YKR037C2.480.006630.00SPC34Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR023W7.601.45E-14DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKL211C6.341.15E-10TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YLR110C5.706.04E-9CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YOL002C5.541.50E-8IZH2Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
YDR283C5.452.45E-8GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR127W5.267.02E-8ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YLR368W4.261.04E-5MDM30F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains
YDR354W4.251.09E-5TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YEL009C4.092.11E-5GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YOR222W4.082.21E-5ODC2Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism
YPR023C4.003.19E-5EAF3Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
YMR316W3.983.46E-5DIA1Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YJR146W_p3.826.74E-5YJR146W_pProtein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
YER090W3.797.43E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR008C_d3.787.87E-5YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_603
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3531.20E-172SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0344828
0.3521.93E-171SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0350877
0.3309.66E-150SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.056338
0.3211.52E-141SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.155844
0.3198.32E-140SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.078125
0.3134.34E-134SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.032967
0.3112.87E-132SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0163934
0.3041.06E-126SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0746269
0.3031.60E-125SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.12987
0.3012.75E-124SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.08
0.3013.72E-124SGTC_2683piperine 63.0 μMTimTec (Pure natural product library)6380240.101449
0.3008.30E-123SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.229508
0.2971.32E-120SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0416667
0.2971.25E-120SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.056338
0.2933.19E-117SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0606061

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5883970-114452.9 μM0.4545451888707ChemDiv (Drug-like library)425.2609434.86425
SGTC_5564058-006351.5 μM0.4035096615692ChemDiv (Drug-like library)417.276424.07924
SGTC_2322741331938.22 μM0.3461541840400Chembridge (Fragment library)254.692782.67323ERAD & cell cycle
SGTC_23276189656200 μM0.3461545349088Chembridge (Fragment library)282.702882.18634
SGTC_1814st05211031.54 μM0.345455674163TimTec (Natural product derivative library)345.57641.94313RNA processing & uracil transport
SGTC_1737st03555769.7 μM0.3333335716916TimTec (Natural product derivative library)286.75284.69802
SGTC_21365328288199.3 μM0.333333262395Chembridge (Fragment library)222.220540.81724
SGTC_22095-(5-methyl-2-thenylidene)-Barbituric acid122.83 μM0.326531616764Chembridge (Fragment library)236.247120.96324
SGTC_2232chlorophetanol41.54 μM0.31818215907Miscellaneous172.608861.93812TRP & mitochondrial translation
SGTC_1817st05211664.4 μM0.3050854361398TimTec (Natural product derivative library)310.73291.85514RNA processing & uracil transport