0302-0167

1-chloro-4-nitroanthracene-9,10-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_612
Screen concentration 22.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 235821
SMILES C1=CC=C2C(=C1)C(=O)C3=C(C=CC(=C3C2=O)Cl)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12
Molecular weight 287.6547
ALogP 3.37
H-bond donor count 0
H-bond acceptor count 4
Response signature copper-dependent oxidative stress

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.73
% growth inhibition (Hom. pool) 9.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 235821
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.03||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:CDC12(YHR107C)|FD-Score:4.76|P-value:9.74E-7|Clearance:0.69||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC24(YAL041W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:6.28|P-value:1.68E-10|Clearance:0.69||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CLP1(YOR250C)|FD-Score:5.6|P-value:1.08E-8|Clearance:0.69||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DBP6(YNR038W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:GCD1(YOR260W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.04||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:NTR2(YKR022C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.04||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:NUF2(YOL069W)|FD-Score:-3.26|P-value:5.51E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUS1(YDL193W)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PCM1(YEL058W)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.13||SGD DESC:Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors Gene:PDI1(YCL043C)|FD-Score:-3.31|P-value:4.61E-4|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PFY1(YOR122C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.26||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:POP4(YBR257W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.06||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPL10(YLR075W)|FD-Score:4.95|P-value:3.70E-7|Clearance:0.69||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPP1(YHR062C)|FD-Score:4.65|P-value:1.64E-6|Clearance:0.69||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RVB2(YPL235W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SPP41(YDR464W)|FD-Score:3.96|P-value:3.75E-5|Clearance:0.38||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:TRE2(YOR256C)|FD-Score:4.84|P-value:6.36E-7|Clearance:0.69||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TUB4(YLR212C)|FD-Score:5.84|P-value:2.58E-9|Clearance:0.69||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:UBC1(YDR177W)|FD-Score:-4.39|P-value:5.66E-6|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:ADE13(YLR359W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.03||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:CDC12(YHR107C)|FD-Score:4.76|P-value:9.74E-7|Clearance:0.69||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC24(YAL041W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:6.28|P-value:1.68E-10|Clearance:0.69||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CLP1(YOR250C)|FD-Score:5.6|P-value:1.08E-8|Clearance:0.69||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DBP6(YNR038W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:GCD1(YOR260W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.04||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:NTR2(YKR022C)|FD-Score:3.58|P-value:1.72E-4|Clearance:0.04||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:NUF2(YOL069W)|FD-Score:-3.26|P-value:5.51E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:NUS1(YDL193W)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PCM1(YEL058W)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.13||SGD DESC:Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors Gene:PDI1(YCL043C)|FD-Score:-3.31|P-value:4.61E-4|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PFY1(YOR122C)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.26||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:POP4(YBR257W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.06||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPL10(YLR075W)|FD-Score:4.95|P-value:3.70E-7|Clearance:0.69||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPP1(YHR062C)|FD-Score:4.65|P-value:1.64E-6|Clearance:0.69||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RVB2(YPL235W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SPP41(YDR464W)|FD-Score:3.96|P-value:3.75E-5|Clearance:0.38||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:TRE2(YOR256C)|FD-Score:4.84|P-value:6.36E-7|Clearance:0.69||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TUB4(YLR212C)|FD-Score:5.84|P-value:2.58E-9|Clearance:0.69||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:UBC1(YDR177W)|FD-Score:-4.39|P-value:5.66E-6|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 235821
Download HOP data (tab-delimited text)  (excel)
Gene:APD1(YBR151W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARN2(YHL047C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ATP14(YLR295C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CAF120(YNL278W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:3.86|P-value:5.68E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB6(YGR109C)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CPR7(YJR032W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTR1(YPR124W)|FD-Score:5.46|P-value:2.44E-8||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CWC21(YDR482C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DAK2(YFL053W)|FD-Score:5.85|P-value:2.41E-9||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DIA4(YHR011W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EFG1(YGR271C-A)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:EMC1(YCL045C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FOB1(YDR110W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FRM2(YCL026C-A)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:FYV12(YOR183W_p)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:HMI1(YOL095C)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HNT3(YOR258W)|FD-Score:4.69|P-value:1.40E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOP1(YIL072W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HPM1(YIL110W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IRC5(YFR038W)|FD-Score:4.35|P-value:6.71E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTR6(YPL053C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LAC1(YKL008C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LEO1(YOR123C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LYS1(YIR034C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAC1(YMR021C)|FD-Score:9.94|P-value:1.40E-23||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRP49(YKL167C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL25(YGR076C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MRPL35(YDR322W)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS35(YGR165W)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC1(YML128C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSM1(YGR171C)|FD-Score:-4.39|P-value:5.65E-6||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:OPI3(YJR073C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCA1(YBR295W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PCL8(YPL219W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEP7(YDR323C)|FD-Score:4.87|P-value:5.56E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO89(YBR296C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PMR1(YGL167C)|FD-Score:8.25|P-value:8.03E-17||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POS5(YPL188W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PUS1(YPL212C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PUS4(YNL292W)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAD18(YCR066W)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD51(YER095W)|FD-Score:4.76|P-value:9.60E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:REG1(YDR028C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGL1(YPL066W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RMD1(YDL001W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RML2(YEL050C)|FD-Score:-3.76|P-value:8.44E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROG1(YGL144C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSM27(YGR215W)|FD-Score:-4.64|P-value:1.70E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:-3.86|P-value:5.71E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SET6(YPL165C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHE10(YGL228W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SIP2(YGL208W)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SKY1(YMR216C)|FD-Score:4.37|P-value:6.14E-6||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLM6(YBR266C_d)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLS1(YLR139C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNA2(YDR525W-A)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNT309(YPR101W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPT3(YDR392W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRL1(YOR247W)|FD-Score:6.49|P-value:4.23E-11||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:TAN1(YGL232W)|FD-Score:15.4|P-value:6.27E-54||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIP41(YPR040W)|FD-Score:4.6|P-value:2.16E-6||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:4.92|P-value:4.43E-7||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TOS8(YGL096W)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TRF5(YNL299W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRI1(YMR233W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:15.6|P-value:2.80E-55||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM8(YDL201W)|FD-Score:5.03|P-value:2.51E-7||SGD DESC:Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRM82(YDR165W)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TSC3(YBR058C-A)|FD-Score:14.1|P-value:2.44E-45||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VAC14(YLR386W)|FD-Score:5.87|P-value:2.18E-9||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAC8(YEL013W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMA13(YPR036W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAP1(YML007W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YCR099C(YCR099C_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Putative protein of unknown function Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YFL015C(YFL015C_d)|FD-Score:4.36|P-value:6.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YHP1(YDR451C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKR045C(YKR045C_p)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL198C(YNL198C_d)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:13.4|P-value:2.87E-41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YRA2(YKL214C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ZRG17(YNR039C)|FD-Score:4.85|P-value:6.06E-7||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency Gene:APD1(YBR151W)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:ARN2(YHL047C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ATP14(YLR295C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CAF120(YNL278W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CCS1(YMR038C)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CLA4(YNL298W)|FD-Score:3.86|P-value:5.68E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB6(YGR109C)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 Gene:CPR7(YJR032W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CTR1(YPR124W)|FD-Score:5.46|P-value:2.44E-8||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CWC21(YDR482C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DAK2(YFL053W)|FD-Score:5.85|P-value:2.41E-9||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DIA4(YHR011W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EFG1(YGR271C-A)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:EMC1(YCL045C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FOB1(YDR110W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Nucleolar protein that binds the rDNA replication fork barrier (RFB) site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to RFB and rDNA repeat segregation; related to retroviral integrases Gene:FRM2(YCL026C-A)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:FYV12(YOR183W_p)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:HMI1(YOL095C)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HNT3(YOR258W)|FD-Score:4.69|P-value:1.40E-6||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOP1(YIL072W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HPM1(YIL110W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:IRC5(YFR038W)|FD-Score:4.35|P-value:6.71E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KTR6(YPL053C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LAC1(YKL008C)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LEO1(YOR123C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LYS1(YIR034C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAC1(YMR021C)|FD-Score:9.94|P-value:1.40E-23||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRP49(YKL167C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL25(YGR076C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MRPL35(YDR322W)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:-4.17|P-value:1.53E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS35(YGR165W)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:-3.11|P-value:9.42E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC1(YML128C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MSM1(YGR171C)|FD-Score:-4.39|P-value:5.65E-6||SGD DESC:Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p Gene:OPI3(YJR073C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCA1(YBR295W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PCL8(YPL219W)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEP7(YDR323C)|FD-Score:4.87|P-value:5.56E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO89(YBR296C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PMR1(YGL167C)|FD-Score:8.25|P-value:8.03E-17||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POS5(YPL188W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PUS1(YPL212C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PUS4(YNL292W)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAD18(YCR066W)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD51(YER095W)|FD-Score:4.76|P-value:9.60E-7||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:REG1(YDR028C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGL1(YPL066W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RMD1(YDL001W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RML2(YEL050C)|FD-Score:-3.76|P-value:8.44E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROG1(YGL144C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:-3.11|P-value:9.40E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSM27(YGR215W)|FD-Score:-4.64|P-value:1.70E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RSM7(YJR113C)|FD-Score:-3.86|P-value:5.71E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SET6(YPL165C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SHE10(YGL228W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SIP2(YGL208W)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SKY1(YMR216C)|FD-Score:4.37|P-value:6.14E-6||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLM6(YBR266C_d)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLS1(YLR139C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNA2(YDR525W-A)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SNT309(YPR101W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SOD1(YJR104C)|FD-Score:4.59|P-value:2.22E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPT3(YDR392W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRL1(YOR247W)|FD-Score:6.49|P-value:4.23E-11||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:TAN1(YGL232W)|FD-Score:15.4|P-value:6.27E-54||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TAT1(YBR069C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIP41(YPR040W)|FD-Score:4.6|P-value:2.16E-6||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:4.92|P-value:4.43E-7||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TOS8(YGL096W)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TRF5(YNL299W)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRI1(YMR233W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:15.6|P-value:2.80E-55||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TRM8(YDL201W)|FD-Score:5.03|P-value:2.51E-7||SGD DESC:Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRM82(YDR165W)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TSC3(YBR058C-A)|FD-Score:14.1|P-value:2.44E-45||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VAC14(YLR386W)|FD-Score:5.87|P-value:2.18E-9||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAC8(YEL013W)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMA13(YPR036W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAP1(YML007W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YCR099C(YCR099C_p)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Putative protein of unknown function Gene:YDR524C-B(YDR524C-B_p)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YFL015C(YFL015C_d)|FD-Score:4.36|P-value:6.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YHP1(YDR451C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKR045C(YKR045C_p)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL067W-B(YNL067W-B_p)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Putative protein of unknown function Gene:YNL184C(YNL184C_p)|FD-Score:-3.16|P-value:7.76E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL198C(YNL198C_d)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:13.4|P-value:2.87E-41||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YRA2(YKL214C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ZRG17(YNR039C)|FD-Score:4.85|P-value:6.06E-7||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR257W6.281.68E-100.69CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YLR212C5.842.58E-90.69TUB4Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
YOR250C5.601.08E-80.69CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YLR075W4.953.70E-70.69RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YOR256C4.846.36E-70.69TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YHR107C4.769.74E-70.69CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YHR062C4.651.64E-60.69RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR464W3.963.75E-50.38SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YKR022C3.581.72E-40.04NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YBR257W3.541.97E-40.06POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YEL058W3.492.43E-40.12PCM1Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
YNR038W3.363.85E-40.00DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YOR260W3.363.87E-40.04GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YLR359W3.324.47E-40.03ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YOR122C3.295.00E-40.26PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR120C15.602.80E-55TRM1tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
YGL232W15.406.27E-54TAN1Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
YBR058C-A14.102.44E-45TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YPR123C_d13.402.87E-41YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YMR021C9.941.40E-23MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YGL167C8.258.03E-17PMR1High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
YOR247W6.494.23E-11SRL1Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
YLR386W5.872.18E-9VAC14Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p
YFL053W5.852.41E-9DAK2Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
YPR124W5.462.44E-8CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YDL201W5.032.51E-7TRM8Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
YNL121C4.924.43E-7TOM70Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
YDR323C4.875.56E-7PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YNR039C4.856.06E-7ZRG17Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency
YCR066W4.856.19E-7RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA

GO enrichment analysis for SGTC_612
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2883.77E-113SGTC_10014048-4355 44.4 μMChemDiv (Drug-like library)218242330.180328copper-dependent oxidative stress
0.2605.75E-92SGTC_3181488-0629 67.2 μMChemDiv (Drug-like library)36625760.172414copper-dependent oxidative stress
0.2541.84E-87SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.129032TSC3-RPN4
0.2501.16E-84SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0344828TSC3-RPN4
0.2441.22E-80SGTC_230213-0011 40.0 μMChemDiv (Drug-like library)6276080.295455copper-dependent oxidative stress
0.2421.71E-79SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.149254TSC3-RPN4
0.2351.14E-74SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.107143TSC3-RPN4
0.2252.19E-68SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.325copper-dependent oxidative stress
0.2196.42E-65SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.183673TSC3-RPN4
0.2111.14E-60SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.181818TSC3-RPN4
0.2074.90E-58SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0517241TSC3-RPN4
0.2069.64E-58SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0909091TSC3-RPN4
0.2061.34E-57SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.169492TSC3-RPN4
0.2061.93E-57SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0724638TSC3-RPN4
0.2002.19E-54SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.0921053TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3710195-00067.36 μM0.4210533928593ChemDiv (Drug-like library)253.209682.70204
SGTC_8201582-006059.9 μM0.3469393848840ChemDiv (Drug-like library)290.661983.50816
SGTC_8470342-02745.54 μM0.34146318097ChemDiv (Drug-like library)218.165641.28204
SGTC_200481-00134.94 μM0.333333182045ChemDiv (Drug-like library)241.070142.68602
SGTC_263alizarin107.93 μM0.3256293Miscellaneous240.210922.32424iron homeostasis
SGTC_2686alizarin95.37 μM0.3256293Miscellaneous240.210922.32424RPP1 & pyrimidine depletion
SGTC_2080521252412.4 μM0.325260183Chembridge (Fragment library)221.639721.36113copper-dependent oxidative stress
SGTC_2130NSC 72.45 μM0.32521448Chembridge (Fragment library)235.66631.89703copper-dependent oxidative stress
SGTC_2507purpurin79.67 μM0.3256683Microsource (Natural product library)256.210322.08235copper-dependent oxidative stress
SGTC_1764st04450293.5 μM0.3170738407TimTec (Natural product derivative library)214.223.27413
SGTC_2674-Nitroquinoline-1-oxide (4-NQO)44.4 nM0.3023265955Miscellaneous190.155541.06603DNA damage response