3914-0030

2-[(6-bromo-4-phenylquinazolin-2-yl)amino]acetic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_613
Screen concentration 117.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1145395
SMILES C1=CC=C(C=C1)C2=NC(=NC3=C2C=C(C=C3)Br)NCC(=O)O
Standardized SMILES OC(=O)CNc1nc(c2ccccc2)c3cc(Br)ccc3n1
Molecular weight 358.1894
ALogP 3.89
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.84
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1145395
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:-3.92|P-value:4.46E-5|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:ORC1(YML065W)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:RPN12(YFR052W)|FD-Score:-4.52|P-value:3.16E-6|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:SPT14(YPL175W)|FD-Score:-4.51|P-value:3.29E-6|Clearance:0||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:YHR020W(YHR020W)|FD-Score:-3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:CDC19(YAL038W)|FD-Score:-3.92|P-value:4.46E-5|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:ORC1(YML065W)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:RPN12(YFR052W)|FD-Score:-4.52|P-value:3.16E-6|Clearance:0||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress Gene:SPT14(YPL175W)|FD-Score:-4.51|P-value:3.29E-6|Clearance:0||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:YHR020W(YHR020W)|FD-Score:-3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1145395
Download HOP data (tab-delimited text)  (excel)
Gene:AIM39(YOL053W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:BMH2(YDR099W)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CAT2(YML042W)|FD-Score:-4.57|P-value:2.46E-6||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CLB4(YLR210W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COA2(YPL189C-A)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CUL3(YGR003W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:EAF3(YPR023C)|FD-Score:-7.61|P-value:1.38E-14||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:ECM27(YJR106W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:FAA3(YIL009W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FCY22(YER060W-A)|FD-Score:-4.9|P-value:4.72E-7||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GAS2(YLR343W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GGA1(YDR358W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLT1(YDL171C)|FD-Score:-5.27|P-value:6.85E-8||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GZF3(YJL110C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HMX1(YLR205C)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:MEI5(YPL121C)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:PBA1(YLR199C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:PCL10(YGL134W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PIC2(YER053C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PPM2(YOL141W)|FD-Score:-5.06|P-value:2.04E-7||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PPS1(YBR276C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:REV1(YOR346W)|FD-Score:-4.59|P-value:2.19E-6||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RIM13(YMR154C)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RNP1(YLL046C)|FD-Score:-4.31|P-value:8.16E-6||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RRT6(YGL146C_p)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RSF2(YJR127C)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:-6.2|P-value:2.91E-10||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SPO75(YLL005C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:STB4(YMR019W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:SUB1(YMR039C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SUL1(YBR294W)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR7(YML052W)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:TIR2(YOR010C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:TSL1(YML100W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:VBA4(YDR119W_p)|FD-Score:-3.82|P-value:6.63E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:WHI4(YDL224C)|FD-Score:-5.21|P-value:9.32E-8||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YBL036C(YBL036C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS Gene:YCL021W-A(YCL021W-A_p)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Putative protein of unknown function Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR491C(YDR491C_d)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL010W(YEL010W_d)|FD-Score:-4.86|P-value:5.77E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL081W(YGL081W_p)|FD-Score:-5.53|P-value:1.59E-8||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGL235W(YGL235W_p)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR093W(YGR093W_p)|FD-Score:-4.5|P-value:3.40E-6||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YKL050C(YKL050C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YKL066W(YKL066W_d)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene YNK1 Gene:YKR023W(YKR023W_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL006W-A(YLL006W-A_p)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR334C(YLR334C_d)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR346C(YLR346C_p)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene Gene:YML083C(YML083C_p)|FD-Score:-4.05|P-value:2.53E-5||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YML084W(YML084W_d)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML119W(YML119W_p)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR111C(YMR111C_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR182W-A(YMR182W-A_p)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Putative protein of unknown function Gene:YNL042W-B(YNL042W-B_p)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Putative protein of unknown function Gene:YOR062C(YOR062C_p)|FD-Score:-5|P-value:2.91E-7||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR093C(YOR093C_p)|FD-Score:-6.29|P-value:1.63E-10||SGD DESC:Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOR345C(YOR345C_d)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPK2(YMR104C)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPL277C(YPL277C_p)|FD-Score:-3.88|P-value:5.12E-5||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:ZRG17(YNR039C)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency Gene:AIM39(YOL053W)|FD-Score:-3.89|P-value:5.02E-5||SGD DESC:Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss Gene:BMH2(YDR099W)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CAT2(YML042W)|FD-Score:-4.57|P-value:2.46E-6||SGD DESC:Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Gene:CLB4(YLR210W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COA2(YPL189C-A)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CUL3(YGR003W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:EAF3(YPR023C)|FD-Score:-7.61|P-value:1.38E-14||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:ECM27(YJR106W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:FAA3(YIL009W)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FCY22(YER060W-A)|FD-Score:-4.9|P-value:4.72E-7||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GAS2(YLR343W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GGA1(YDR358W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLT1(YDL171C)|FD-Score:-5.27|P-value:6.85E-8||SGD DESC:NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source Gene:GZF3(YJL110C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HMX1(YLR205C)|FD-Score:-3.53|P-value:2.07E-4||SGD DESC:ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants Gene:MEI5(YPL121C)|FD-Score:-3.17|P-value:7.54E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:PBA1(YLR199C)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly Gene:PCL10(YGL134W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PIC2(YER053C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PPM2(YOL141W)|FD-Score:-5.06|P-value:2.04E-7||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PPS1(YBR276C)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:REV1(YOR346W)|FD-Score:-4.59|P-value:2.19E-6||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RIM13(YMR154C)|FD-Score:-3.11|P-value:9.44E-4||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RNP1(YLL046C)|FD-Score:-4.31|P-value:8.16E-6||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RRT6(YGL146C_p)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins Gene:RSF2(YJR127C)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SET6(YPL165C)|FD-Score:-6.2|P-value:2.91E-10||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SPO75(YLL005C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis Gene:STB4(YMR019W)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:SUB1(YMR039C)|FD-Score:-3.93|P-value:4.28E-5||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:SUL1(YBR294W)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR7(YML052W)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:TIR2(YOR010C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:TSL1(YML100W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:VBA4(YDR119W_p)|FD-Score:-3.82|P-value:6.63E-5||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:WHI4(YDL224C)|FD-Score:-5.21|P-value:9.32E-8||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YBL036C(YBL036C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS Gene:YCL021W-A(YCL021W-A_p)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Putative protein of unknown function Gene:YCR102W-A(YCR102W-A_d)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR491C(YDR491C_d)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL010W(YEL010W_d)|FD-Score:-4.86|P-value:5.77E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL081W(YGL081W_p)|FD-Score:-5.53|P-value:1.59E-8||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YGL235W(YGL235W_p)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR093W(YGR093W_p)|FD-Score:-4.5|P-value:3.40E-6||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YKL050C(YKL050C)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Protein of unknown function; the YKL050W protein is a target of the SCFCdc4 ubiquitin ligase complex and YKL050W transcription is regulated by Azf1p Gene:YKL066W(YKL066W_d)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene YNK1 Gene:YKR023W(YKR023W_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL006W-A(YLL006W-A_p)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YLR334C(YLR334C_d)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here Gene:YLR346C(YLR346C_p)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene Gene:YML083C(YML083C_p)|FD-Score:-4.05|P-value:2.53E-5||SGD DESC:Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions Gene:YML084W(YML084W_d)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML119W(YML119W_p)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR111C(YMR111C_p)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress Gene:YMR182W-A(YMR182W-A_p)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Putative protein of unknown function Gene:YNL042W-B(YNL042W-B_p)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Putative protein of unknown function Gene:YOR062C(YOR062C_p)|FD-Score:-5|P-value:2.91E-7||SGD DESC:Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YOR093C(YOR093C_p)|FD-Score:-6.29|P-value:1.63E-10||SGD DESC:Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOR345C(YOR345C_d)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPK2(YMR104C)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Protein kinase with similarity to serine/threonine protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; homolog of mammalian kinase SGK; YPK2 has a paralog, YPK1, that arose from the whole genome duplication Gene:YPL277C(YPL277C_p)|FD-Score:-3.88|P-value:5.12E-5||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:ZRG17(YNR039C)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W2.820.002370.23LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YMR208W2.590.004810.08ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YBR265W2.510.006120.03TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YDR373W2.480.006590.07FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YDL132W2.410.007970.00CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YDR302W2.410.008060.01GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YMR093W2.390.008360.04UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGL225W2.350.009330.01VRG4Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi
YNL150W_d2.340.009630.07YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YHR083W2.270.011500.15SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YOR056C2.120.017000.01NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YIL031W2.110.017600.00ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YPR108W2.100.017700.01RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YPL083C2.100.018100.02SEN54Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YOR278W2.080.018900.04HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR293C3.070.00107SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YGR263C2.910.00179SAY1Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
YKL055C2.860.00209OAR1Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p
YDR354W2.820.00244TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YOR027W2.790.00266STI1Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
YDR181C2.720.00331SAS4Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
YFR036W2.670.00378CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YDR483W2.660.00390KRE2Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
YDR136C_d2.630.00427VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YDR005C2.610.00456MAF1Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
YPL157W2.590.00474TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YJL099W2.550.00538CHS6Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
YER161C2.530.00578SPT2Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins
YML121W2.520.00591GTR1Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
YGR255C2.480.00656COQ6Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)

GO enrichment analysis for SGTC_613
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2714.83E-98SGTC_6140462-0418 107.0 μMChemDiv (Drug-like library)51883030.057971
0.2486.94E-83SGTC_7504073-0104 132.0 μMChemDiv (Drug-like library)223309570.125
0.1722.75E-40SGTC_1107amlodipine 47.0 μMNIH Clinical Collection21620.136364
0.1094.62E-17SGTC_32069114350 49.5 μMChembridge (Drug-like library)170872430.147059
0.0881.39E-11SGTC_6304356-0227 183.0 μMChemDiv (Drug-like library)7793120.130435
0.0681.67E-7SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.180328DNA intercalators
0.0596.55E-6SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0714286DNA intercalators
0.0597.22E-6SGTC_615k087-0205 167.0 μMChemDiv (Drug-like library)96427730.0873786
0.0589.25E-6SGTC_23669070441 60.6 μMChembridge (Fragment library)68372350.114754
0.0589.37E-6SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.147059DNA intercalators
0.0534.46E-5SGTC_2405ticlatone 3.5 μMMiscellaneous62580.0806452
0.0501.16E-4SGTC_9143820-6779 408.0 μMChemDiv (Drug-like library)28934810.136986
0.0482.55E-4SGTC_2654protoveratrine a 100.0 μMMicrosource (Natural product library)57022780.0403226
0.0455.70E-4SGTC_6811642-0006 26.6 μMChemDiv (Drug-like library)44465300.075
0.0456.39E-4SGTC_74000s-0487 160.3 μMChemDiv (Drug-like library)6775480.166667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1913914-012366.51 μM0.663144412ChemDiv (Drug-like library)358.232443.9362460S ribosome export
SGTC_12063978-0017132 μM0.524593873677ChemDiv (Drug-like library)469.331143.82816ERG2
SGTC_1833914-005130.55 μM0.4745761596232ChemDiv (Drug-like library)392.67754.62460S ribosome export
SGTC_12931000-1278195 μM0.446429678847ChemDiv (Drug-like library)342.186683.16912
SGTC_6653914-0008307 μM0.388061069549ChemDiv (Drug-like library)404.461663.56616
SGTC_348k064-001210.7 μM0.2931035066323ChemDiv (Drug-like library)367.2143835.8571360S ribosome export
SGTC_761k216-0224480 μM0.2758621340404ChemDiv (Drug-like library)362.3289865.22816
SGTC_350133-005464.07 μM0.27272799677ChemDiv (Drug-like library)276.12863.23512
SGTC_8390122-0028230 μM0.263158759448ChemDiv (Drug-like library)265.149023.91711RSC complex & mRNA processing
SGTC_540159-00559 μM0.2575763319099ChemDiv (Drug-like library)530.476949.51910ERG2