1652-1634

(4E)-5-methyl-2-phenyl-4-(1,3-thiazol-2-ylhydrazinylidene)pyrazol-3-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_619
Screen concentration 108.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5400454
SMILES CC1=NN(C(=O)C1=NNC2=NC=CS2)C3=CC=CC=C3
Standardized SMILES Cc1nn(c(O)c1N=Nc2nccs2)c3ccccc3
Molecular weight 285.3243
ALogP 1.82
H-bond donor count 1
H-bond acceptor count 6
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.42
% growth inhibition (Hom. pool) 10.77


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5400454
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.28||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC39(YCR093W)|FD-Score:-3.52|P-value:2.16E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:FOL3(YMR113W)|FD-Score:3.13|P-value:8.87E-4|Clearance:0.16||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GPN2(YOR262W)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HRT1(YOL133W)|FD-Score:-4.04|P-value:2.68E-5|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MEX67(YPL169C)|FD-Score:4.92|P-value:4.37E-7|Clearance:0.86||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOC3(YLR002C)|FD-Score:-4.01|P-value:3.04E-5|Clearance:0||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:NOP14(YDL148C)|FD-Score:5.22|P-value:9.18E-8|Clearance:0.12||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:POL31(YJR006W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.01||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PRP3(YDR473C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.23||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFA1(YAR007C)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RFC5(YBR087W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.04||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIB7(YBR153W)|FD-Score:6.03|P-value:8.36E-10|Clearance:0.81||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:SEC13(YLR208W)|FD-Score:10.3|P-value:4.12E-25|Clearance:4.26||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:5.1|P-value:1.70E-7|Clearance:0.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC62(YPL094C)|FD-Score:4.06|P-value:2.44E-5|Clearance:0.38||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:ARC19(YKL013C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.28||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC39(YCR093W)|FD-Score:-3.52|P-value:2.16E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:FOL3(YMR113W)|FD-Score:3.13|P-value:8.87E-4|Clearance:0.16||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:GPN2(YOR262W)|FD-Score:-3.26|P-value:5.52E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HRT1(YOL133W)|FD-Score:-4.04|P-value:2.68E-5|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MEX67(YPL169C)|FD-Score:4.92|P-value:4.37E-7|Clearance:0.86||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOC3(YLR002C)|FD-Score:-4.01|P-value:3.04E-5|Clearance:0||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:NOP14(YDL148C)|FD-Score:5.22|P-value:9.18E-8|Clearance:0.12||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:POL31(YJR006W)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.01||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PRP3(YDR473C)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.23||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RFA1(YAR007C)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RFC5(YBR087W)|FD-Score:3.69|P-value:1.14E-4|Clearance:0.04||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIB7(YBR153W)|FD-Score:6.03|P-value:8.36E-10|Clearance:0.81||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:SEC13(YLR208W)|FD-Score:10.3|P-value:4.12E-25|Clearance:4.26||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:5.1|P-value:1.70E-7|Clearance:0.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC62(YPL094C)|FD-Score:4.06|P-value:2.44E-5|Clearance:0.38||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5400454
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:APS3(YJL024C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARF3(YOR094W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ARN2(YHL047C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARR3(YPR201W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ASE1(YOR058C)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATG16(YMR159C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BCH1(YMR237W)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BIM1(YER016W)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BST1(YFL025C)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCS1(YMR038C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-4.72|P-value:1.18E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DIA2(YOR080W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:DSE2(YHR143W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUG2(YBR281C)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELC1(YPL046C)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:GAL11(YOL051W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAT4(YIR013C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDT1(YBR187W_p)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:IXR1(YKL032C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:KAP114(YGL241W)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:LGE1(YPL055C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LYS4(YDR234W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MSN1(YOL116W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NUT1(YGL151W)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OAF3(YKR064W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ODC2(YOR222W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:OPI8(YKR035C_d)|FD-Score:5.28|P-value:6.32E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OTU2(YHL013C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PEX5(YDR244W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:QRI7(YDL104C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RDS1(YCR106W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REG1(YDR028C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX2(YLR059C)|FD-Score:6.66|P-value:1.34E-11||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RHR2(YIL053W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:-4.59|P-value:2.23E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RSA3(YLR221C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SDC25(YLL016W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SDS24(YBR214W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication Gene:SFT2(YBL102W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHG1(YBR258C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres Gene:SLM5(YCR024C)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SMK1(YPR054W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SNF8(YPL002C)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPO21(YOL091W)|FD-Score:-5.41|P-value:3.13E-8||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SUC2(YIL162W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TDH2(YJR009C)|FD-Score:-5.17|P-value:1.19E-7||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI4(YGR144W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TOM5(YPR133W-A)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPK2(YPL203W)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP1(YDR007W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUS1(YLR425W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:VAM6(YDL077C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS4(YPR173C)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YAL065C(YAL065C_p)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL012C(YBL012C_d)|FD-Score:8.8|P-value:6.76E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YDR467C(YDR467C_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGR266W(YGR266W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL215C(YJL215C_d)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:-4.4|P-value:5.34E-6||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL118W(YKL118W_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YMR102C(YMR102C_p)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR119W-A(YMR119W-A_d)|FD-Score:4.51|P-value:3.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR326C(YMR326C_d)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YNL235C(YNL235C_d)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YPL216W(YPL216W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YRR1(YOR162C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:AAC1(YMR056C)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:APS3(YJL024C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ARF3(YOR094W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ARN2(YHL047C)|FD-Score:4.71|P-value:1.23E-6||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO1(YDR127W)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARR3(YPR201W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ASE1(YOR058C)|FD-Score:4.92|P-value:4.35E-7||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:ATG16(YMR159C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BCH1(YMR237W)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BIM1(YER016W)|FD-Score:5.34|P-value:4.71E-8||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BST1(YFL025C)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCS1(YMR038C)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-4.72|P-value:1.18E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DIA2(YOR080W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:DSE2(YHR143W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUG2(YBR281C)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ELC1(YPL046C)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:GAL11(YOL051W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAT4(YIR013C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GDT1(YBR187W_p)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:IXR1(YKL032C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:KAP114(YGL241W)|FD-Score:-3.15|P-value:8.23E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:LGE1(YPL055C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LYS4(YDR234W)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MSN1(YOL116W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NUT1(YGL151W)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OAF3(YKR064W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ODC2(YOR222W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:OPI8(YKR035C_d)|FD-Score:5.28|P-value:6.32E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OTU2(YHL013C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PEX5(YDR244W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:QRI7(YDL104C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification Gene:RDS1(YCR106W)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:REG1(YDR028C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:REX2(YLR059C)|FD-Score:6.66|P-value:1.34E-11||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RHR2(YIL053W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:-4.59|P-value:2.23E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RSA3(YLR221C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SDC25(YLL016W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SDS24(YBR214W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication Gene:SFT2(YBL102W)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHG1(YBR258C)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres Gene:SLM5(YCR024C)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SMK1(YPR054W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SNF8(YPL002C)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPO21(YOL091W)|FD-Score:-5.41|P-value:3.13E-8||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SUC2(YIL162W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TDH2(YJR009C)|FD-Score:-5.17|P-value:1.19E-7||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI4(YGR144W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TOM5(YPR133W-A)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPK2(YPL203W)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP1(YDR007W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:-3.94|P-value:4.09E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-4.04|P-value:2.64E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUS1(YLR425W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:VAM6(YDL077C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:3.87|P-value:5.50E-5||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS4(YPR173C)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YAL065C(YAL065C_p)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL012C(YBL012C_d)|FD-Score:8.8|P-value:6.76E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR174C(YBR174C_d)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YDR467C(YDR467C_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL045C(YEL045C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGR266W(YGR266W)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YJL215C(YJL215C_d)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR084W(YJR084W)|FD-Score:-4.4|P-value:5.34E-6||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YKL118W(YKL118W_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YMR102C(YMR102C_p)|FD-Score:4.69|P-value:1.37E-6||SGD DESC:Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene Gene:YMR119W-A(YMR119W-A_d)|FD-Score:4.51|P-value:3.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR326C(YMR326C_d)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:YNL235C(YNL235C_d)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YPL216W(YPL216W_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YRR1(YOR162C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W10.304.12E-254.26SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR153W6.038.36E-100.81RIB7Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
YDL148C5.229.18E-80.12NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YDL195W5.101.70E-70.18SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPL169C4.924.37E-70.86MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YPL094C4.062.44E-50.38SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YBR087W3.691.14E-40.04RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YJR006W3.651.31E-40.01POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YKL013C3.641.38E-40.28ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YDR473C3.353.98E-40.23PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YMR113W3.138.87E-40.16FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YJL143W2.970.001510.00TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YJL032W_d2.960.001530.15YJL032W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
YBL076C2.810.002450.18ILS1Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
YFL017C2.630.004300.04GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL012C_d8.806.76E-19YBL012C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR059C6.661.34E-11REX23'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease
YFL025C5.531.59E-8BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YER016W5.344.71E-8BIM1Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
YKR035C_d5.286.32E-8OPI8_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
YMR326C_d5.151.28E-7YMR326C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13
YMR237W5.012.74E-7BCH1Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication
YOR058C4.924.35E-7ASE1Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
YCR106W4.751.02E-6RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YHL047C4.711.23E-6ARN2Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C
YMR102C_p4.691.37E-6YMR102C_pProtein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
YBR281C4.612.06E-6DUG2Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
YMR119W-A_d4.513.22E-6YMR119W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR162C4.503.47E-6YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YPR173C4.483.81E-6VPS4AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism

GO enrichment analysis for SGTC_619
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2151.54E-62SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.101449
0.1892.16E-48SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.114286
0.1885.53E-48SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.138889
0.1762.10E-42SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.0945946
0.1631.36E-36SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.119403
0.1578.48E-34SGTC_31979113331 49.5 μMChembridge (Drug-like library)166104590.160494
0.1504.75E-31SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.15625
0.1316.12E-24SGTC_14163966-0321 25.3 μMChemDiv (Drug-like library)5825300.166667
0.1317.80E-24SGTC_9933909-8757 62.7 μMChemDiv (Drug-like library)42883240.161765
0.1231.80E-21SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.1125
0.1232.16E-21SGTC_8550428-0020 25.0 μMChemDiv (Drug-like library)68124140.129032Golgi
0.1195.65E-20SGTC_391gliotoxin 8.3 μMMiscellaneous62230.0714286
0.1125.86E-18SGTC_890302-0250 142.1 μMChemDiv (Drug-like library)42035180.0597015
0.1094.10E-17SGTC_12680828-0275 2.5 μMChemDiv (Drug-like library)7453370.101449
0.0952.85E-13SGTC_3111315-0136 58.0 μMChemDiv (Drug-like library)67592070.0609756

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23196033012152.6 μM0.315789847246Chembridge (Fragment library)219.262960.86524tubulin folding & SWR complex
SGTC_2061524588480.95 μM0.309091573863Chembridge (Fragment library)210.276041.56514tubulin folding & SWR complex
SGTC_21886126565140.68 μM0.305085874518Chembridge (Fragment library)208.237020.86114mitochondrial processes
SGTC_7641951-2361146 μM0.303035438930ChemDiv (Drug-like library)401.226052.75325
SGTC_10583448-542597.3 μM0.2982467279705ChemDiv (Drug-like library)262.305863.71602
SGTC_1390099-0334718.62 μM0.29310397690ChemDiv (Drug-like library)278.305262.50303
SGTC_7961234-002070.7 μM0.2812546495034ChemDiv (Drug-like library)345.32084.1902SWF1 & branched chain AA biosynthesis
SGTC_22267298446200 μM0.2238813283010Chembridge (Fragment library)287.379982.70104
SGTC_5321548-0774215 μM0.2207795932993ChemDiv (Drug-like library)418.468322.56725
SGTC_1101ebselen5.29 μM0.2181823194NIH Clinical Collection274.176663.22701