0109-0052

4-amino-N-[4-[6-[(4-aminobenzoyl)amino]-1H-benzimidazol-2-yl]phenyl]benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_620
Screen concentration 432.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 493037
SMILES C1=CC(=CC=C1C2=NC3=C(N2)C=C(C=C3)NC(=O)C4=CC=C(C=C4)N)NC(=O)C5=CC=C(C=C5)N
Standardized SMILES Nc1ccc(cc1)C(=O)Nc2ccc(cc2)c3nc4cc(NC(=O)c5ccc(N)cc5)ccc4[nH]3
Molecular weight 462.5026
ALogP 3.35
H-bond donor count 5
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.56
% growth inhibition (Hom. pool) 4.5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 493037
Download HIP data (tab-delimited text)  (excel)
Gene:CCT5(YJR064W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:4.95|P-value:3.70E-7|Clearance:0.74||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC53(YDL132W)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.05||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CSE4(YKL049C)|FD-Score:-3.39|P-value:3.51E-4|Clearance:0||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:FOL3(YMR113W)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.01||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HEM2(YGL040C)|FD-Score:-3.24|P-value:5.94E-4|Clearance:0||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:LSM5(YER146W)|FD-Score:-3.84|P-value:6.08E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:PDS1(YDR113C)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.08||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:RPB10(YOR210W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.58||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RRP4(YHR069C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP45(YDR280W)|FD-Score:3.25|P-value:5.81E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RVB2(YPL235W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.09||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SMD2(YLR275W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.18||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SPC24(YMR117C)|FD-Score:-4.1|P-value:2.02E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:CCT5(YJR064W)|FD-Score:3.64|P-value:1.39E-4|Clearance:0.06||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:4.95|P-value:3.70E-7|Clearance:0.74||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC53(YDL132W)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.05||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CSE4(YKL049C)|FD-Score:-3.39|P-value:3.51E-4|Clearance:0||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:FOL3(YMR113W)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.01||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HEM2(YGL040C)|FD-Score:-3.24|P-value:5.94E-4|Clearance:0||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:LSM5(YER146W)|FD-Score:-3.84|P-value:6.08E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:PDS1(YDR113C)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.08||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:RPB10(YOR210W)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.58||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RRP4(YHR069C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP45(YDR280W)|FD-Score:3.25|P-value:5.81E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:RVB2(YPL235W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.09||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SMD2(YLR275W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.18||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SPC24(YMR117C)|FD-Score:-4.1|P-value:2.02E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 493037
Download HOP data (tab-delimited text)  (excel)
Gene:ALY1(YKR021W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:BIM1(YER016W)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BNI1(YNL271C)|FD-Score:7.1|P-value:6.25E-13||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:CCW12(YLR110C)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDH1(YGL003C)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CHL1(YPL008W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:COS1(YNL336W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DBP7(YKR024C)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DDI3(YNL335W_p)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DFG16(YOR030W)|FD-Score:5.35|P-value:4.47E-8||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DST1(YGL043W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EEB1(YPL095C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ENB1(YOL158C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERV46(YAL042W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FMP25(YLR077W)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV8(YGR196C)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL10(YBR019C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GNT1(YOR320C)|FD-Score:-6.6|P-value:2.05E-11||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:HKR1(YDR420W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HMO1(YDR174W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HUR1(YGL168W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HVG1(YER039C)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IRE1(YHR079C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:KTI12(YKL110C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LTP1(YPR073C)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:NCA2(YPR155C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:PAF1(YBR279W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PTH1(YHR189W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD10(YML095C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD54(YGL163C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:REH1(YLR387C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RPL21A(YBR191W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:4.3|P-value:8.42E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSC1(YGR056W)|FD-Score:5.99|P-value:1.03E-9||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAP4(YGL229C)|FD-Score:-5.22|P-value:9.16E-8||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SAT4(YCR008W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SBE22(YHR103W)|FD-Score:4.3|P-value:8.50E-6||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SED1(YDR077W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SPT8(YLR055C)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB2(YHR041C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRB5(YGR104C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:STL1(YDR536W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:URA8(YJR103W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VBA4(YDR119W_p)|FD-Score:8.32|P-value:4.47E-17||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VMA22(YHR060W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS9(YML097C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:XBP1(YIL101C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR134W(YBR134W_d)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCH1(YGR203W)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR161W(YDR161W_p)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YEL010W(YEL010W_d)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR018C(YGR018C_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YJR084W(YJR084W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YLR001C(YLR001C_p)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR122C(YLR122C_d)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR312C(YLR312C_p)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Putative protein of unknown function Gene:YNL010W(YNL010W_p)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNR062C(YNR062C_p)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Putative membrane protein of unknown function Gene:YPL025C(YPL025C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR109W(YPR109W_p)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPS7(YDR349C)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:ALY1(YKR021W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:BIM1(YER016W)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BNI1(YNL271C)|FD-Score:7.1|P-value:6.25E-13||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:CCW12(YLR110C)|FD-Score:5.38|P-value:3.82E-8||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDH1(YGL003C)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CHL1(YPL008W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome Gene:COS1(YNL336W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:DBP7(YKR024C)|FD-Score:4.94|P-value:3.95E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DDI3(YNL335W_p)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:DFG16(YOR030W)|FD-Score:5.35|P-value:4.47E-8||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DST1(YGL043W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EEB1(YPL095C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ENB1(YOL158C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERV46(YAL042W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FMP25(YLR077W)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:FYV8(YGR196C)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GAL10(YBR019C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GNT1(YOR320C)|FD-Score:-6.6|P-value:2.05E-11||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:HKR1(YDR420W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HMO1(YDR174W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HUR1(YGL168W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HVG1(YER039C)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function, has homology to Vrg4p Gene:IRE1(YHR079C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:KTI12(YKL110C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LTP1(YPR073C)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:NCA2(YPR155C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:PAF1(YBR279W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PTH1(YHR189W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD10(YML095C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD54(YGL163C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:REH1(YLR387C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RPL21A(YBR191W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS11B(YBR048W)|FD-Score:4.3|P-value:8.42E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RPS21B(YJL136C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSC1(YGR056W)|FD-Score:5.99|P-value:1.03E-9||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SAP4(YGL229C)|FD-Score:-5.22|P-value:9.16E-8||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SAT4(YCR008W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SBE22(YHR103W)|FD-Score:4.3|P-value:8.50E-6||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SED1(YDR077W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SPT8(YLR055C)|FD-Score:3.85|P-value:5.88E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB2(YHR041C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRB5(YGR104C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:STL1(YDR536W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:URA8(YJR103W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VBA4(YDR119W_p)|FD-Score:8.32|P-value:4.47E-17||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VMA22(YHR060W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS9(YML097C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:XBP1(YIL101C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR134W(YBR134W_d)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCH1(YGR203W)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR161W(YDR161W_p)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YEL010W(YEL010W_d)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR018C(YGR018C_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YJR084W(YJR084W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome Gene:YLR001C(YLR001C_p)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR122C(YLR122C_d)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR312C(YLR312C_p)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:Putative protein of unknown function Gene:YNL010W(YNL010W_p)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNR062C(YNR062C_p)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Putative membrane protein of unknown function Gene:YPL025C(YPL025C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR109W(YPR109W_p)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPS7(YDR349C)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR107C4.953.70E-70.74CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YOR210W4.211.26E-50.58RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YJR064W3.641.39E-40.06CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YHR069C3.581.73E-40.06RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YLR275W3.522.19E-40.18SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YPL235W3.344.20E-40.09RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YDR280W3.255.81E-40.05RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YMR113W3.197.01E-40.01FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YDL132W3.187.37E-40.05CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YDR113C3.138.69E-40.08PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YER104W3.050.001150.01RTT105Protein with a role in regulation of Ty1 transposition
YPL238C_d3.030.001210.07YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YJL109C2.960.001520.00UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YDR212W2.960.001530.02TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YER025W2.940.001620.12GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR119W_p8.324.47E-17VBA4_pProtein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
YNL271C7.106.25E-13BNI1Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
YGR056W5.991.03E-9RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YLR110C5.383.82E-8CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YOR030W5.354.47E-8DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
YKR024C4.943.95E-7DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YPR073C4.934.02E-7LTP1Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
YBR134W_d4.444.40E-6YBR134W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR161W_p4.327.88E-6YDR161W_pPutative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
YBR048W4.308.42E-6RPS11BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
YHR103W4.308.50E-6SBE22Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
YHR041C4.241.10E-5SRB2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
YFL036W4.221.24E-5RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YGL163C4.211.27E-5RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
YDR174W4.131.83E-5HMO1Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase

GO enrichment analysis for SGTC_620
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0823.15E-10SGTC_625k213-0058 121.0 μMChemDiv (Drug-like library)36554200.243243translation
0.0672.53E-7SGTC_8003448-7362 155.0 μMChemDiv (Drug-like library)159919130.141026translation
0.0663.39E-7SGTC_13983558-0053 112.0 μMChemDiv (Drug-like library)45323690.149425
0.0664.00E-7SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.0895522RNA processing & uracil transport
0.0664.09E-7SGTC_1773st048542 73.6 μMTimTec (Natural product derivative library)32371380.0547945
0.0648.61E-7SGTC_8324083-0019 164.0 μMChemDiv (Drug-like library)19907580.246753translation
0.0631.17E-6SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.0547945RPP1 & pyrimidine depletion
0.0631.37E-6SGTC_3832324-0395 7.4 μMChemDiv (Drug-like library)31464210.0352941azole & statin
0.0622.00E-6SGTC_14884013-0813 75.9 μMChemDiv (Drug-like library)29100570.05RPP1 & pyrimidine depletion
0.0612.36E-6SGTC_31259127534 49.5 μMChembridge (Drug-like library)16392350.166667
0.0571.10E-5SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.0526316heat shock/prefoldin
0.0552.07E-5SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.175676RNA processing & uracil transport
0.0552.69E-5SGTC_22837944793 200.0 μMChembridge (Fragment library)29705330.112676
0.0534.55E-5SGTC_401neomycin 50.0 μMMiscellaneous83780.0123457
0.0525.49E-5SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous59550.0625DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_6990375-0592111 μM0.5762578ChemDiv (Drug-like library)251.326283.71822iron homeostasis
SGTC_21826325192200 μM0.411765736248Chembridge (Fragment library)226.273762.35422Golgi
SGTC_8380109-020371.4 μM0.372549759422ChemDiv (Drug-like library)208.258483.75611
SGTC_5382922-083853.3 μM0.345455777039ChemDiv (Drug-like library)259.305223.43222Golgi
SGTC_29087930301100 μM0.342223178Chembridge (Drug-like library)387.431143.00533
SGTC_660109-018742.4 μM0.333333759417ChemDiv (Drug-like library)228.676963.93511
SGTC_20134024628200 μM0.3272735230760Chembridge (Fragment library)226.273762.35422
SGTC_10553448-067286 μM0.315789748378ChemDiv (Drug-like library)302.369723.92412
SGTC_3118912425049.47 μM0.3114751504786Chembridge (Drug-like library)317.16944.12912
SGTC_990109-0120622.8 μM0.309091759414ChemDiv (Drug-like library)321.126163.25713