k048-0052

3-[2-[(3,5-dibromo-6-oxocyclohexa-2,4-dien-1-ylidene)methyl]hydrazinyl]benzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_624
Screen concentration 28.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6835531
SMILES C1=CC(=CC(=C1)NNC=C2C=C(C=C(C2=O)Br)Br)C(=O)O
Standardized SMILES OC(=O)c1cccc(NN=Cc2cc(Br)cc(Br)c2O)c1
Molecular weight 414.0488
ALogP 2.31
H-bond donor count 3
H-bond acceptor count 5
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.53
% growth inhibition (Hom. pool) 8.76


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6835531
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:6.12|P-value:4.65E-10|Clearance:0.94||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DAM1(YGR113W)|FD-Score:-3.92|P-value:4.48E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:ERG11(YHR007C)|FD-Score:7.13|P-value:4.87E-13|Clearance:1.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:LCD1(YDR499W)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LSM2(YBL026W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.08||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.02||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP56(YLR197W)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.02||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RFA1(YAR007C)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.06||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RHO1(YPR165W)|FD-Score:5.18|P-value:1.11E-7|Clearance:0.98||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB2(YOR151C)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.23||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPB8(YOR224C)|FD-Score:3.96|P-value:3.77E-5|Clearance:0.24||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RSC58(YLR033W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.21||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SRB4(YER022W)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TAF12(YDR145W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.31||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:3.72|P-value:9.99E-5|Clearance:0.02||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ARP7(YPR034W)|FD-Score:6.12|P-value:4.65E-10|Clearance:0.94||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DAM1(YGR113W)|FD-Score:-3.92|P-value:4.48E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:ERG11(YHR007C)|FD-Score:7.13|P-value:4.87E-13|Clearance:1.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:LCD1(YDR499W)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LSM2(YBL026W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.08||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.02||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP56(YLR197W)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.02||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:RFA1(YAR007C)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.06||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RHO1(YPR165W)|FD-Score:5.18|P-value:1.11E-7|Clearance:0.98||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB2(YOR151C)|FD-Score:3.45|P-value:2.83E-4|Clearance:0.23||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPB8(YOR224C)|FD-Score:3.96|P-value:3.77E-5|Clearance:0.24||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RSC58(YLR033W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.21||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.21|P-value:6.55E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SRB4(YER022W)|FD-Score:-3.23|P-value:6.22E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TAF12(YDR145W)|FD-Score:3.11|P-value:9.35E-4|Clearance:0.31||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIF35(YDR429C)|FD-Score:3.72|P-value:9.99E-5|Clearance:0.02||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6835531
Download HOP data (tab-delimited text)  (excel)
Gene:ATG3(YNR007C)|FD-Score:4.54|P-value:2.88E-6||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CAP1(YKL007W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CMC4(YMR194C-B)|FD-Score:-3.92|P-value:4.47E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:COG6(YNL041C)|FD-Score:6.77|P-value:6.29E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:10.1|P-value:1.82E-24||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:10.8|P-value:1.72E-27||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ9(YLR201C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:DCR2(YLR361C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START Gene:DIA3(YDL024C)|FD-Score:5.42|P-value:2.95E-8||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DST1(YGL043W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:DUN1(YDL101C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM19(YLR390W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERV15(YBR210W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FAR3(YMR052W)|FD-Score:5.94|P-value:1.41E-9||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FLC1(YPL221W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FRT1(YOR324C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GCV3(YAL044C)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:HUA1(YGR268C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IMO32(YGR031W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:KEL1(YHR158C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:MFT1(YML062C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNT4(YNR059W)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MSA2(YKR077W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSH5(YDL154W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NUC1(YJL208C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:PAA1(YDR071C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PCL10(YGL134W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PCS60(YBR222C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PDR18(YNR070W_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PET10(YKR046C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX18(YHR160C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX5(YDR244W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PUS1(YPL212C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RGP1(YDR137W)|FD-Score:6.52|P-value:3.44E-11||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM9(YMR063W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RSM25(YIL093C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SEC22(YLR268W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHE9(YDR393W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SIS2(YKR072C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SNC1(YAL030W)|FD-Score:5.35|P-value:4.33E-8||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SUC2(YIL162W)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TVP38(YKR088C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UTR4(YEL038W)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VBA2(YBR293W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPH2(YKL119C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS21(YOR089C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS35(YJL154C)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS52(YDR484W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBL070C(YBL070C_d)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL046W(YCL046W_d)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL009C(YDL009C_p)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YHK8(YHR048W_p)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIL029C(YIL029C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL027C(YJL027C_p)|FD-Score:4.68|P-value:1.42E-6||SGD DESC:Putative protein of unknown function Gene:YMR265C(YMR265C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function Gene:YNL190W(YNL190W_p)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNR014W(YNR014W_p)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL037C(YOL037C_d)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:ATG3(YNR007C)|FD-Score:4.54|P-value:2.88E-6||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CAP1(YKL007W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CMC4(YMR194C-B)|FD-Score:-3.92|P-value:4.47E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:COG6(YNL041C)|FD-Score:6.77|P-value:6.29E-12||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:10.1|P-value:1.82E-24||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:10.8|P-value:1.72E-27||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ9(YLR201C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes Gene:DCR2(YLR361C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START Gene:DIA3(YDL024C)|FD-Score:5.42|P-value:2.95E-8||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:DST1(YGL043W)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:DUN1(YDL101C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM19(YLR390W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERV15(YBR210W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FAR3(YMR052W)|FD-Score:5.94|P-value:1.41E-9||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FLC1(YPL221W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FRT1(YOR324C)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GCV3(YAL044C)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:HUA1(YGR268C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IMO32(YGR031W)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:KEL1(YHR158C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:MFT1(YML062C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNT4(YNR059W)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MSA2(YKR077W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSH5(YDL154W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:NUC1(YJL208C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:PAA1(YDR071C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PCL10(YGL134W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PCS60(YBR222C)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PDR18(YNR070W_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PET10(YKR046C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX18(YHR160C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PEX5(YDR244W)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PUS1(YPL212C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RGP1(YDR137W)|FD-Score:6.52|P-value:3.44E-11||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIM9(YMR063W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RSM25(YIL093C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SEC22(YLR268W)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHE9(YDR393W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SIS2(YKR072C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SNC1(YAL030W)|FD-Score:5.35|P-value:4.33E-8||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SUC2(YIL162W)|FD-Score:-3.94|P-value:4.10E-5||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TVP38(YKR088C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UTR4(YEL038W)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus Gene:VBA2(YBR293W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPH2(YKL119C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS21(YOR089C)|FD-Score:3.46|P-value:2.65E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS35(YJL154C)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS52(YDR484W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBL070C(YBL070C_d)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL046W(YCL046W_d)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL009C(YDL009C_p)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YHK8(YHR048W_p)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIL029C(YIL029C_p)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL027C(YJL027C_p)|FD-Score:4.68|P-value:1.42E-6||SGD DESC:Putative protein of unknown function Gene:YMR265C(YMR265C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function Gene:YNL190W(YNL190W_p)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNR014W(YNR014W_p)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL037C(YOL037C_d)|FD-Score:-3.18|P-value:7.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR007C7.134.87E-131.01ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YPR034W6.124.65E-100.94ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YPR165W5.181.11E-70.98RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YLR033W4.201.36E-50.21RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YLR197W3.983.41E-50.02NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YOR224C3.963.77E-50.24RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YDR429C3.729.99E-50.02TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YCR052W3.701.09E-40.01RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YIL048W3.681.15E-40.02NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YBL026W3.661.27E-40.08LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YDL196W_d3.581.71E-40.14YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YOR151C3.452.83E-40.23RPB2RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
YFR037C3.216.55E-40.04RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YAR007C3.177.55E-40.06RFA1Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination
YDR145W3.119.35E-40.31TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML071C10.801.72E-27COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL005C10.101.82E-24COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL041C6.776.29E-12COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR137W6.523.44E-11RGP1Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
YMR052W5.941.41E-9FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YDL024C5.422.95E-8DIA3Protein of unknown function, involved in invasive and pseudohyphal growth
YAL030W5.354.33E-8SNC1Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication
YJL027C_p4.681.42E-6YJL027C_pPutative protein of unknown function
YNR007C4.542.88E-6ATG3E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
YAL044C4.356.93E-6GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YKR088C4.042.64E-5TVP38Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
YDL009C_p4.042.68E-5YDL009C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
YNR064C4.032.78E-5YNR064CEpoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
YDR222W_p3.953.84E-5YDR222W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YNR014W_p3.944.10E-5YNR014W_pPutative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication

GO enrichment analysis for SGTC_624
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2968.24E-120SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.25Golgi
0.2795.97E-106SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.407407Golgi
0.2681.02E-97SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.344262RSC & ERG11
0.2483.30E-83SGTC_7830848-0141 92.7 μMChemDiv (Drug-like library)67550760.31746RSC & ERG11
0.2448.18E-81SGTC_7461095-0025 46.6 μMChemDiv (Drug-like library)67492860.344828RSC & ERG11
0.2421.11E-79SGTC_590k048-0076 22.4 μMChemDiv (Drug-like library)68328140.362069RSC & ERG11
0.2381.26E-76SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0571429Golgi
0.2311.97E-72SGTC_185k048-0037 25.5 μMChemDiv (Drug-like library)68275890.627451RSC & ERG11
0.2147.56E-62SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.33871RSC & ERG11
0.2138.28E-61SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.277778Golgi
0.2093.81E-59SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0967742RSC complex & mRNA processing
0.2052.88E-57SGTC_5571315-0110 16.2 μMChemDiv (Drug-like library)68338010.173913Golgi
0.2041.94E-56SGTC_5660958-0068 16.5 μMChemDiv (Drug-like library)68153650.491803RSC & ERG11
0.1924.64E-50SGTC_329k048-0121 21.3 μMChemDiv (Drug-like library)68329020.407407RSC & ERG11
0.1911.24E-49SGTC_8550428-0020 25.0 μMChemDiv (Drug-like library)68124140.186441Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_352k048-00075.11 μM0.6666676811911ChemDiv (Drug-like library)404.484363.34223ERAD & cell cycle
SGTC_185k048-003725.49 μM0.6274516827589ChemDiv (Drug-like library)414.04882.30735RSC & ERG11
SGTC_342k048-008823.3 μM0.5370376820570ChemDiv (Drug-like library)413.060743.1224RSC & ERG11
SGTC_551k048-002834.9 μM0.56836383ChemDiv (Drug-like library)447.1201033.5234
SGTC_5660958-006816.5 μM0.4918036815365ChemDiv (Drug-like library)492.1358233.99424RSC & ERG11
SGTC_4300958-011516.4 μM0.4603176833180ChemDiv (Drug-like library)508.590424.45323
SGTC_13531498-12068.11 μM0.4230776756808ChemDiv (Drug-like library)371.024062.76223mitochondrial response to ROS
SGTC_329k048-012121.3 μM0.4074076832902ChemDiv (Drug-like library)480.921193.58212RSC & ERG11
SGTC_1162k048-013020.1 μM0.4074076797406ChemDiv (Drug-like library)431.120624.52312Golgi
SGTC_331k048-013324.5 μM0.3928576813582ChemDiv (Drug-like library)356.012721.85413RSC & ERG11