3448-6466

N-quinolin-3-ylthiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_629
Screen concentration 76.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 573744
SMILES C1=CC=C2C(=C1)C=C(C=N2)NC(=O)C3=CC=CS3
Standardized SMILES O=C(Nc1cnc2ccccc2c1)c3cccs3
Molecular weight 254.307
ALogP 2.75
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 37.19
% growth inhibition (Hom. pool) 20.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 573744
Download HIP data (tab-delimited text)  (excel)
Gene:DFR1(YOR236W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.06||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:ERG13(YML126C)|FD-Score:3.29|P-value:5.01E-4|Clearance:0.02||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:GLC7(YER133W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.02||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KEG1(YFR042W)|FD-Score:4.52|P-value:3.15E-6|Clearance:0.85||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:NTR2(YKR022C)|FD-Score:3.14|P-value:8.38E-4|Clearance:0.11||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:PKC1(YBL105C)|FD-Score:-4.56|P-value:2.51E-6|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP28(YDR243C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.2||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RPA135(YPR010C)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.07||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RPP1(YHR062C)|FD-Score:6.06|P-value:7.00E-10|Clearance:1.54||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC31(YDL195W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.02||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC1(YFL008W)|FD-Score:3.15|P-value:8.18E-4|Clearance:0.01||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SPC105(YGL093W)|FD-Score:-3.46|P-value:2.73E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPP381(YBR152W)|FD-Score:-3.77|P-value:8.01E-5|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TIM44(YIL022W)|FD-Score:3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TOM40(YMR203W)|FD-Score:-3.1|P-value:9.80E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YGR114C(YGR114C_d)|FD-Score:3.27|P-value:5.30E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YRA1(YDR381W)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:DFR1(YOR236W)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.06||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:ERG13(YML126C)|FD-Score:3.29|P-value:5.01E-4|Clearance:0.02||SGD DESC:3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis Gene:GLC7(YER133W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.02||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:KEG1(YFR042W)|FD-Score:4.52|P-value:3.15E-6|Clearance:0.85||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:NTR2(YKR022C)|FD-Score:3.14|P-value:8.38E-4|Clearance:0.11||SGD DESC:Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly Gene:PKC1(YBL105C)|FD-Score:-4.56|P-value:2.51E-6|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP28(YDR243C)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.2||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RPA135(YPR010C)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.07||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RPP1(YHR062C)|FD-Score:6.06|P-value:7.00E-10|Clearance:1.54||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC31(YDL195W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.02||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC1(YFL008W)|FD-Score:3.15|P-value:8.18E-4|Clearance:0.01||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SPC105(YGL093W)|FD-Score:-3.46|P-value:2.73E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPP381(YBR152W)|FD-Score:-3.77|P-value:8.01E-5|Clearance:0||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TIM44(YIL022W)|FD-Score:3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:TOM40(YMR203W)|FD-Score:-3.1|P-value:9.80E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YGR114C(YGR114C_d)|FD-Score:3.27|P-value:5.30E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YRA1(YDR381W)|FD-Score:-3.39|P-value:3.49E-4|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 573744
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:5.73|P-value:4.97E-9||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADH3(YMR083W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:AEP1(YMR064W)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AEP2(YMR282C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM26(YKL037W)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ARR1(YPR199C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATF2(YGR177C)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking Gene:BCK1(YJL095W)|FD-Score:-3.85|P-value:5.92E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:-4.32|P-value:7.74E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:4.37|P-value:6.10E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM18(YDR125C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ENT3(YJR125C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FMP25(YLR077W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GAC1(YOR178C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GLC8(YMR311C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPD2(YOL059W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HCR1(YLR192C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IKI1(YHR187W)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:MEF1(YLR069C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM101(YJR144W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MOT3(YMR070W)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRH4(YGL064C)|FD-Score:4.79|P-value:8.45E-7||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPL23(YOR150W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:5|P-value:2.80E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSF1(YPR047W)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSH5(YDL154W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSW1(YDR268W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NCL1(YBL024W)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NVJ1(YHR195W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PET111(YMR257C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PKH3(YDR466W)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PNP1(YLR209C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PRM7(YDL039C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:PRO2(YOR323C)|FD-Score:-3.86|P-value:5.66E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RML2(YEL050C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPE1(YJL121C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL12B(YDR418W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL15B(YMR121C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPL40A(YIL148W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN10(YHR200W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPN4(YDL020C)|FD-Score:-6.24|P-value:2.25E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS4B(YHR203C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAT4(YCR008W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SAY1(YGR263C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SIN4(YNL236W)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIS2(YKR072C)|FD-Score:5.55|P-value:1.42E-8||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SPT3(YDR392W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SUV3(YPL029W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAN1(YGL232W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TOM5(YPR133W-A)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPO4(YOR273C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP3(YKL211C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBC7(YMR022W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBP6(YFR010W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:ULS1(YOR191W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:URA2(YJL130C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VPS35(YJL154C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS68(YOL129W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR087C-A(YCR087C-A_p)|FD-Score:4.27|P-value:9.88E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR509W(YDR509W_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL218W(YGL218W_d)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR064W(YGR064W_d)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL060W(YIL060W_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YML053C(YML053C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL195C(YNL195C_p)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:AAC1(YMR056C)|FD-Score:5.73|P-value:4.97E-9||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ADH3(YMR083W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:AEP1(YMR064W)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AEP2(YMR282C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM26(YKL037W)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ARR1(YPR199C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATF2(YGR177C)|FD-Score:-3.97|P-value:3.59E-5||SGD DESC:Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking Gene:BCK1(YJL095W)|FD-Score:-3.85|P-value:5.92E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BST1(YFL025C)|FD-Score:-4.32|P-value:7.74E-6||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCR4(YAL021C)|FD-Score:4.37|P-value:6.10E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COG7(YGL005C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM18(YDR125C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication Gene:ENT3(YJR125C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FMP25(YLR077W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria Gene:GAC1(YOR178C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GLC8(YMR311C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GPD2(YOL059W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:HCR1(YLR192C)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:IKI1(YHR187W)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:MEF1(YLR069C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MGM101(YJR144W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MOT3(YMR070W)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRH4(YGL064C)|FD-Score:4.79|P-value:8.45E-7||SGD DESC:Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function Gene:MRPL23(YOR150W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:5|P-value:2.80E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MRPS5(YBR251W)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSF1(YPR047W)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:MSH5(YDL154W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans Gene:MSW1(YDR268W)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NCL1(YBL024W)|FD-Score:-4.58|P-value:2.35E-6||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NVJ1(YHR195W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:PET111(YMR257C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PKH3(YDR466W)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PNP1(YLR209C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PRM7(YDL039C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:PRO2(YOR323C)|FD-Score:-3.86|P-value:5.66E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RML2(YEL050C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPE1(YJL121C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL12B(YDR418W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL15B(YMR121C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RPL40A(YIL148W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN10(YHR200W)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPN4(YDL020C)|FD-Score:-6.24|P-value:2.25E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS4B(YHR203C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:SAT4(YCR008W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p Gene:SAY1(YGR263C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SIN4(YNL236W)|FD-Score:4.95|P-value:3.78E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIS2(YKR072C)|FD-Score:5.55|P-value:1.42E-8||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SPT3(YDR392W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SUV3(YPL029W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAN1(YGL232W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TOM5(YPR133W-A)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TPO4(YOR273C)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP3(YKL211C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBC7(YMR022W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBP6(YFR010W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:ULS1(YOR191W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:URA2(YJL130C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VPS35(YJL154C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS68(YOL129W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR087C-A(YCR087C-A_p)|FD-Score:4.27|P-value:9.88E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR509W(YDR509W_d)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL218W(YGL218W_d)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR064W(YGR064W_d)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL060W(YIL060W_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YML053C(YML053C_p)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL195C(YNL195C_p)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C6.067.00E-101.54RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YFR042W4.523.15E-60.85KEG1Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
YDL195W3.671.22E-40.02SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR010C3.651.33E-40.07RPA135RNA polymerase I second largest subunit A135
YDR243C3.571.77E-40.20PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YER133W3.373.71E-40.02GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YOR236W3.354.00E-40.06DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YML126C3.295.01E-40.02ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YGR114C_d3.275.30E-40.12YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YIL022W3.158.06E-40.00TIM44Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
YFL008W3.158.18E-40.01SMC1Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
YKR022C3.148.38E-40.11NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YJR067C3.030.001210.05YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YLR310C2.990.001420.01CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YMR288W2.970.001490.13HSH155U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR056C5.734.97E-9AAC1Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YKR072C5.551.42E-8SIS2Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YIL060W_p5.151.31E-7YIL060W_pPutative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
YGR165W5.002.80E-7MRPS35Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
YNL236W4.953.78E-7SIN4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
YGL064C4.798.45E-7MRH4Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function
YMR083W4.681.46E-6ADH3Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production
YHR187W4.493.60E-6IKI1Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
YLR384C4.424.85E-6IKI3Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
YLR069C4.425.02E-6MEF1Mitochondrial elongation factor involved in translational elongation
YAL021C4.376.10E-6CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YBR251W4.289.26E-6MRPS5Mitochondrial ribosomal protein of the small subunit
YCR087C-A_p4.279.88E-6YCR087C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
YCR008W4.251.08E-5SAT4Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; partially redundant with Hal5p; has similarity to Npr1p
YGR064W_d4.141.71E-5YGR064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C

GO enrichment analysis for SGTC_629
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1552.82E-33SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.118421mitochondrial stress
0.1504.31E-31SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0689655TRP & mitochondrial translation
0.1262.74E-22SGTC_2113455-0679 143.5 μMChemDiv (Drug-like library)7486490.106061
0.1202.40E-20SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0757576mitochondrial stress
0.1194.56E-20SGTC_1623st003709 20.3 μMTimTec (Natural product derivative library)161926180.0978261calcium & mitochondrial duress
0.1181.08E-19SGTC_32329132874 49.5 μMChembridge (Drug-like library)37849400.259259RPP1 & pyrimidine depletion
0.1171.87E-19SGTC_6001495-0418 41.6 μMChemDiv (Drug-like library)53777680.129032RPP1 & pyrimidine depletion
0.1172.11E-19SGTC_3141671-0008 2.7 μMChemDiv (Drug-like library)39130600.0684932
0.1164.13E-19SGTC_3101273-0058 1.1 μMChemDiv (Drug-like library)27342320.0163934mitochondrial stress
0.1159.52E-19SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.0909091RPP1 & pyrimidine depletion
0.1132.38E-18SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0493827RPP1 & pyrimidine depletion
0.1111.09E-17SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.101695TRP & mitochondrial translation
0.1102.67E-17SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0588235
0.1072.08E-16SGTC_1766st045414 44.8 μMTimTec (Natural product derivative library)2539600.115942RPP1 & pyrimidine depletion
0.1056.03E-16SGTC_13853091-4834 14.1 μMChemDiv (Drug-like library)40728140.123077RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2061524588480.95 μM0.488889573863Chembridge (Fragment library)210.276041.56514tubulin folding & SWR complex
SGTC_2810795987145.46 μM0.4655172974535Chembridge (Drug-like library)333.40694.35213copper-dependent oxidative stress
SGTC_3187910939449.47 μM0.44230817098090Chembridge (Drug-like library)247.312822.913Golgi
SGTC_20535265679138 μM0.428571764595Chembridge (Fragment library)233.286242.55113
SGTC_3189911068249.47 μM0.41071417122418Chembridge (Drug-like library)273.35013.61413
SGTC_22647948757110.76 μM0.4081632971411Chembridge (Fragment library)252.312721.35624
SGTC_2276794751342.08 μM0.42105100Chembridge (Fragment library)234.297442.23314
SGTC_14123909-8563178 μM0.39436623767530ChemDiv (Drug-like library)449.525422.45416
SGTC_9143820-6779408 μM0.3928572893481ChemDiv (Drug-like library)392.450884.69725
SGTC_3118912425049.47 μM0.3818181504786Chembridge (Drug-like library)317.16944.12912