k072-0230

methyl 3-[(2Z)-2-(pyridin-3-ylmethylidene)hydrazinyl]thiophene-2-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_631
Screen concentration 41.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 5773147
SMILES COC(=O)C1=C(C=CS1)NN=CC2=CN=CC=C2
Standardized SMILES COC(=O)c1sccc1NN=Cc2cccnc2
Molecular weight 261.2996
ALogP 2.11
H-bond donor count 1
H-bond acceptor count 6
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.95
% growth inhibition (Hom. pool) 10.99


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5773147
Download HIP data (tab-delimited text)  (excel)
Gene:CDC45(YLR103C)|FD-Score:3.28|P-value:5.11E-4|Clearance:0.09||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:ENP2(YGR145W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:FCP1(YMR277W)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.01||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LCD1(YDR499W)|FD-Score:-3.77|P-value:8.30E-5|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LSG1(YGL099W)|FD-Score:8.38|P-value:2.56E-17|Clearance:2.73||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NAB3(YPL190C)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.16||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NMD3(YHR170W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.33||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRP16(YKR086W)|FD-Score:3.72|P-value:9.97E-5|Clearance:0.1||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:PSF1(YDR013W)|FD-Score:3.84|P-value:6.21E-5|Clearance:0.07||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PUP3(YER094C)|FD-Score:3.9|P-value:4.89E-5|Clearance:0.06||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RPB3(YIL021W)|FD-Score:5.66|P-value:7.76E-9|Clearance:1.74||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC13(YLR208W)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.01||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TFB3(YDR460W)|FD-Score:3.77|P-value:8.09E-5|Clearance:0.05||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:UTP10(YJL109C)|FD-Score:3.72|P-value:9.85E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:CDC45(YLR103C)|FD-Score:3.28|P-value:5.11E-4|Clearance:0.09||SGD DESC:DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes Gene:ENP2(YGR145W)|FD-Score:-3.31|P-value:4.60E-4|Clearance:0||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:FCP1(YMR277W)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.01||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:LCD1(YDR499W)|FD-Score:-3.77|P-value:8.30E-5|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:LSG1(YGL099W)|FD-Score:8.38|P-value:2.56E-17|Clearance:2.73||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NAB3(YPL190C)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.16||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:NMD3(YHR170W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.33||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PRP16(YKR086W)|FD-Score:3.72|P-value:9.97E-5|Clearance:0.1||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:PSF1(YDR013W)|FD-Score:3.84|P-value:6.21E-5|Clearance:0.07||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PUP3(YER094C)|FD-Score:3.9|P-value:4.89E-5|Clearance:0.06||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RPB3(YIL021W)|FD-Score:5.66|P-value:7.76E-9|Clearance:1.74||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC13(YLR208W)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.01||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TFB3(YDR460W)|FD-Score:3.77|P-value:8.09E-5|Clearance:0.05||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:UTP10(YJL109C)|FD-Score:3.72|P-value:9.85E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5773147
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:5.29|P-value:6.07E-8||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ARG81(YML099C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ARO9(YHR137W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG5(YPL149W)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BNI1(YNL271C)|FD-Score:6.02|P-value:8.69E-10||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BNI4(YNL233W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CCW12(YLR110C)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS3(YBR023C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CMS1(YLR003C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COA2(YPL189C-A)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG6(YNL041C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSS1(YMR287C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:FET4(YMR319C)|FD-Score:4.37|P-value:6.23E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FUM1(YPL262W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GDS1(YOR355W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GRE2(YOL151W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HAA1(YPR008W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HXT10(YFL011W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IMA1(YGR287C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC14(YOR135C_d)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITT1(YML068W)|FD-Score:4.3|P-value:8.37E-6||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:MRPL17(YNL252C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST1(YKL194C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NCL1(YBL024W)|FD-Score:-4.84|P-value:6.34E-7||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NTH2(YBR001C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUT1(YGL151W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OAF1(YAL051W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:OPT2(YPR194C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:ORM2(YLR350W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSH2(YDL019C)|FD-Score:-4.82|P-value:7.24E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OYE2(YHR179W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCL10(YGL134W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PEP7(YDR323C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PGD1(YGL025C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POC4(YPL144W)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:RAD51(YER095W)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD57(YDR004W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RCK1(YGL158W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RIF1(YBR275C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RKR1(YMR247C)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPS11A(YDR025W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:SCS22(YBL091C-A)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:STP1(YDR463W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPN1(YGL186C)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRS85(YDR108W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBA3(YPR066W)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ULS1(YOR191W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UME6(YDR207C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VBA5(YKR105C_p)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VPS69(YPR087W_d)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WSS1(YHR134W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YER097W(YER097W_d)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Putative protein of unknown function Gene:YHL037C(YHL037C_d)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHP1(YDR451C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR138C(YHR138C_p)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL089W(YIL089W)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YLR184W(YLR184W_d)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR365W(YLR365W_d)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Gene:YLR413W(YLR413W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YMR034C(YMR034C_p)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL102C(YPL102C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.93|P-value:4.25E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR109W(YPR109W_p)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YRM1(YOR172W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes Gene:ADH4(YGL256W)|FD-Score:5.29|P-value:6.07E-8||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ARG81(YML099C)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ARO9(YHR137W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ATG5(YPL149W)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BNI1(YNL271C)|FD-Score:6.02|P-value:8.69E-10||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BNI4(YNL233W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD16(YEL029C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CCW12(YLR110C)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS3(YBR023C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CMS1(YLR003C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COA2(YPL189C-A)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:COG6(YNL041C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSS1(YMR287C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:FET4(YMR319C)|FD-Score:4.37|P-value:6.23E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FUM1(YPL262W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GDS1(YOR355W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GRE2(YOL151W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HAA1(YPR008W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HXT10(YFL011W)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IMA1(YGR287C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRC14(YOR135C_d)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITT1(YML068W)|FD-Score:4.3|P-value:8.37E-6||SGD DESC:Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins Gene:MRPL17(YNL252C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST1(YKL194C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NCL1(YBL024W)|FD-Score:-4.84|P-value:6.34E-7||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NTH2(YBR001C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUT1(YGL151W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription Gene:OAF1(YAL051W)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:4.38|P-value:6.06E-6||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:OPT2(YPR194C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:ORM2(YLR350W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSH2(YDL019C)|FD-Score:-4.82|P-value:7.24E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OYE2(YHR179W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCL10(YGL134W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PEP7(YDR323C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PGD1(YGL025C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POC4(YPL144W)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:RAD51(YER095W)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD57(YDR004W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RCK1(YGL158W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RIF1(YBR275C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RKR1(YMR247C)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RPS11A(YDR025W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:SCS22(YBL091C-A)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:STP1(YDR463W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TOS6(YNL300W_p)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPN1(YGL186C)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRS85(YDR108W)|FD-Score:3.86|P-value:5.75E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBA3(YPR066W)|FD-Score:-3.74|P-value:9.19E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ULS1(YOR191W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UME6(YDR207C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VBA5(YKR105C_p)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VPS69(YPR087W_d)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:WSS1(YHR134W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YER097W(YER097W_d)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR121W-A(YGR121W-A_p)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Putative protein of unknown function Gene:YHL037C(YHL037C_d)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHP1(YDR451C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR138C(YHR138C_p)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL089W(YIL089W)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YLR184W(YLR184W_d)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR365W(YLR365W_d)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene Gene:YLR413W(YLR413W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YMR034C(YMR034C_p)|FD-Score:-3.16|P-value:7.96E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPL102C(YPL102C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.93|P-value:4.25E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR109W(YPR109W_p)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YRM1(YOR172W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W8.382.56E-172.73LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YIL021W5.667.76E-91.74RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YMR277W3.924.47E-50.01FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YLR208W3.914.66E-50.01SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YER094C3.904.89E-50.06PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YDR013W3.846.21E-50.07PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YDR460W3.778.09E-50.05TFB3Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
YJL109C3.729.85E-50.00UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YKR086W3.729.97E-50.10PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YHR170W3.621.49E-40.33NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YLR103C3.285.11E-40.09CDC45DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
YPL190C3.197.00E-40.17NAB3RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
YDL195W3.030.001220.01SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YER159C3.020.001260.00BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YDR464W3.020.001270.07SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL271C6.028.69E-10BNI1Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
YKR019C5.891.98E-9IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YGL256W5.296.07E-8ADH4Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
YGR121W-A_p4.973.34E-7YGR121W-A_pPutative protein of unknown function
YPL149W4.602.09E-6ATG5Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation
YKR105C_p4.434.64E-6VBA5_pPlasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p
YPR194C4.434.76E-6OPT2Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles
YER095W4.415.19E-6RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YGL186C4.405.37E-6TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YKR087C4.386.06E-6OMA1Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes
YMR319C4.376.23E-6FET4Low-affinity Fe(II) transporter of the plasma membrane
YPL144W4.327.96E-6POC4Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
YML068W4.308.37E-6ITT1Protein that modulates the efficiency of translation termination, interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
YHR137W4.171.50E-5ARO9Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism
YDR207C4.111.99E-5UME6Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p

GO enrichment analysis for SGTC_631
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1602.94E-35SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.30158760S ribosome export
0.1441.17E-28SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.08450760S ribosome export
0.1403.37E-27SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.080459860S ribosome export
0.1381.21E-26SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.13333360S ribosome export
0.1301.32E-23SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.0694444
0.1293.65E-23SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.138462Golgi
0.1284.33E-23SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.081081160S ribosome export
0.1284.48E-23SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.14666760S ribosome export
0.1285.40E-23SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.17647160S ribosome export
0.1232.99E-21SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.274194heme biosynthesis & mitochondrial translocase
0.1211.11E-20SGTC_9761348-1067 47.6 μMChemDiv (Drug-like library)53106710.0860S ribosome export
0.1194.31E-20SGTC_5382922-0838 53.3 μMChemDiv (Drug-like library)7770390.0694444Golgi
0.1195.88E-20SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.115942
0.1171.67E-19SGTC_23307979373 200.0 μMChembridge (Fragment library)18630070.097222260S ribosome export
0.1172.22E-19SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.075949460S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1033k072-010221.3 μM0.755965931ChemDiv (Drug-like library)261.299642.1061660S ribosome export
SGTC_575k072-024611.9 μM0.5423736796779ChemDiv (Drug-like library)299.347622.25826
SGTC_1165k072-027530.3 μM0.5098046153523ChemDiv (Drug-like library)211.262422.78413
SGTC_2424553792859.2 μM0.4137935382802Miscellaneous254.31032.92315
SGTC_5371334-0398209 μM0.3442625846986ChemDiv (Drug-like library)270.283263.06225
SGTC_8304076-028657.8 μM0.3291146866659ChemDiv (Drug-like library)411.455844.26825translation
SGTC_3034909042149.47 μM0.32835817173761Chembridge (Drug-like library)304.364142.381560S ribosome export
SGTC_11841269-1718139 μM0.3225815403821ChemDiv (Drug-like library)260.311582.6214
SGTC_6390833-0042114 μM0.3114755978911ChemDiv (Drug-like library)252.311042.71303
SGTC_22857945142200 μM0.3064522970608Chembridge (Fragment library)247.273060.25224