0437-0093

benzyl N-[2,2,2-trichloro-1-[(2-methoxy-5-nitrophenyl)carbamothioylamino]ethyl]carbamate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_633
Screen concentration 97.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 2831118
SMILES COC1=C(C=C(C=C1)[N+](=O)[O-])NC(=S)NC(C(Cl)(Cl)Cl)NC(=O)OCC2=CC=CC=C2
Standardized SMILES COc1ccc(cc1NC(=S)NC(NC(=O)OCc2ccccc2)C(Cl)(Cl)Cl)[N+](=O)[O-]
Molecular weight 507.7754
ALogP 5.61
H-bond donor count 3
H-bond acceptor count 6
Response signature DNA intercalators

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.68
% growth inhibition (Hom. pool) 2.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2831118
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.19||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:ERG12(YMR208W)|FD-Score:7.52|P-value:2.77E-14|Clearance:1.57||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG25(YGR060W)|FD-Score:-3.54|P-value:1.97E-4|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:GPI13(YLL031C)|FD-Score:3.17|P-value:7.50E-4|Clearance:0.25||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:HEM4(YOR278W)|FD-Score:4.18|P-value:1.46E-5|Clearance:0.24||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MAK21(YDR060W)|FD-Score:6.64|P-value:1.58E-11|Clearance:1.57||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MEX67(YPL169C)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.22||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NHP2(YDL208W)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:PFS2(YNL317W)|FD-Score:3.91|P-value:4.61E-5|Clearance:0.1||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PMI40(YER003C)|FD-Score:3.94|P-value:4.07E-5|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:POP8(YBL018C)|FD-Score:3.75|P-value:8.67E-5|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PSF1(YDR013W)|FD-Score:-3.53|P-value:2.05E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PTI1(YGR156W)|FD-Score:3.81|P-value:7.03E-5|Clearance:0.01||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RFA1(YAR007C)|FD-Score:-4.11|P-value:1.96E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RFC4(YOL094C)|FD-Score:4.31|P-value:8.29E-6|Clearance:0.06||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPS3(YNL178W)|FD-Score:3.25|P-value:5.82E-4|Clearance:0.07||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT2(YDL007W)|FD-Score:-3.19|P-value:7.03E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RRS1(YOR294W)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.07||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TFC6(YDR362C)|FD-Score:5.07|P-value:1.98E-7|Clearance:0.76||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:USO1(YDL058W)|FD-Score:8.66|P-value:2.33E-18|Clearance:1.57||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:YEF3(YLR249W)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.04||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YPL044C(YPL044C_d)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W Gene:ARC15(YIL062C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.19||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:ERG12(YMR208W)|FD-Score:7.52|P-value:2.77E-14|Clearance:1.57||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG25(YGR060W)|FD-Score:-3.54|P-value:1.97E-4|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:GPI13(YLL031C)|FD-Score:3.17|P-value:7.50E-4|Clearance:0.25||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:HEM4(YOR278W)|FD-Score:4.18|P-value:1.46E-5|Clearance:0.24||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MAK21(YDR060W)|FD-Score:6.64|P-value:1.58E-11|Clearance:1.57||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:MEX67(YPL169C)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.22||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NHP2(YDL208W)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:PFS2(YNL317W)|FD-Score:3.91|P-value:4.61E-5|Clearance:0.1||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:PMI40(YER003C)|FD-Score:3.94|P-value:4.07E-5|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:POP8(YBL018C)|FD-Score:3.75|P-value:8.67E-5|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PSF1(YDR013W)|FD-Score:-3.53|P-value:2.05E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PTI1(YGR156W)|FD-Score:3.81|P-value:7.03E-5|Clearance:0.01||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RFA1(YAR007C)|FD-Score:-4.11|P-value:1.96E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination Gene:RFC4(YOL094C)|FD-Score:4.31|P-value:8.29E-6|Clearance:0.06||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPS3(YNL178W)|FD-Score:3.25|P-value:5.82E-4|Clearance:0.07||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT2(YDL007W)|FD-Score:-3.19|P-value:7.03E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RRS1(YOR294W)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.07||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TFC6(YDR362C)|FD-Score:5.07|P-value:1.98E-7|Clearance:0.76||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:USO1(YDL058W)|FD-Score:8.66|P-value:2.33E-18|Clearance:1.57||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:YEF3(YLR249W)|FD-Score:3.79|P-value:7.39E-5|Clearance:0.04||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YPL044C(YPL044C_d)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2831118
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ABP140(YOR239W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ADR1(YDR216W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ANR2(YKL047W_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:APD1(YBR151W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:AQY2(YLL052C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ASI1(YMR119W)|FD-Score:8.1|P-value:2.66E-16||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BDS1(YOL164W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BIT61(YJL058C)|FD-Score:-3.78|P-value:7.72E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BNA7(YDR428C)|FD-Score:-4.35|P-value:6.88E-6||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BXI1(YNL305C)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAP1(YKL007W)|FD-Score:4.85|P-value:6.20E-7||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHS6(YJL099W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CTK1(YKL139W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAN1(YJR150C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DDP1(YOR163W)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:ECM2(YBR065C)|FD-Score:-5.53|P-value:1.57E-8||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:EGD1(YPL037C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:EMP70(YLR083C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERV46(YAL042W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FAT3(YKL187C_p)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FZO1(YBR179C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GZF3(YJL110C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:KTR1(YOR099W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:MBB1(YJL199C_d)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MET14(YKL001C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MRP21(YBL090W)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPS28(YDR337W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB1(YOR188W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NCL1(YBL024W)|FD-Score:-5.02|P-value:2.61E-7||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NIS1(YNL078W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:OAF3(YKR064W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:OM14(YBR230C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OXP1(YKL215C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAM1(YDR251W)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PIB2(YGL023C)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PLB3(YOL011W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMP2(YEL017C-A)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:POF1(YCL047C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PPM2(YOL141W)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PSP1(YDR505C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:RAD9(YDR217C)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RDS1(YCR106W)|FD-Score:7.29|P-value:1.50E-13||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RIM8(YGL045W)|FD-Score:-4.01|P-value:3.05E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RLF2(YPR018W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL23B(YER117W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTK1(YDL025C)|FD-Score:-4.93|P-value:4.21E-7||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RUB1(YDR139C)|FD-Score:-3.92|P-value:4.52E-5||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SDH2(YLL041C)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SDT1(YGL224C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SHE3(YBR130C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SNO1(YMR095C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SVF1(YDR346C)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TDH2(YJR009C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:VIK1(YPL253C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VMA5(YKL080W)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YCL042W(YCL042W_p)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR064C(YCR064C_d)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YEA6(YEL006W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGL039W(YGL039W)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YGL177W(YGL177W_d)|FD-Score:7.1|P-value:6.34E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR078W(YHR078W_p)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YHR131C(YHR131C_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YJR079W(YJR079W_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YLL007C(YLL007C_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YNL122C(YNL122C_p)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YPL277C(YPL277C_p)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR150W(YPR150W_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:AAT2(YLR027C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ABP140(YOR239W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ADR1(YDR216W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ANR2(YKL047W_p)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:APD1(YBR151W)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:AQY2(YLL052C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ASI1(YMR119W)|FD-Score:8.1|P-value:2.66E-16||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:BDS1(YOL164W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BIT61(YJL058C)|FD-Score:-3.78|P-value:7.72E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BNA7(YDR428C)|FD-Score:-4.35|P-value:6.88E-6||SGD DESC:Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine Gene:BXI1(YNL305C)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAP1(YKL007W)|FD-Score:4.85|P-value:6.20E-7||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CHS6(YJL099W)|FD-Score:3.87|P-value:5.44E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CTK1(YKL139W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DAN1(YJR150C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DDP1(YOR163W)|FD-Score:-3.78|P-value:7.76E-5||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:ECM2(YBR065C)|FD-Score:-5.53|P-value:1.57E-8||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:EGD1(YPL037C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:EMP70(YLR083C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ERV46(YAL042W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FAT3(YKL187C_p)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FZO1(YBR179C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GZF3(YJL110C)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:KTR1(YOR099W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:MBB1(YJL199C_d)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MET14(YKL001C)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Gene:MRP21(YBL090W)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPS28(YDR337W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB1(YOR188W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NCL1(YBL024W)|FD-Score:-5.02|P-value:2.61E-7||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NIS1(YNL078W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:OAF3(YKR064W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:OM14(YBR230C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OXP1(YKL215C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAM1(YDR251W)|FD-Score:5.42|P-value:2.94E-8||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PIB2(YGL023C)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PLB3(YOL011W)|FD-Score:-3.32|P-value:4.51E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMP2(YEL017C-A)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:POF1(YCL047C)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PPM2(YOL141W)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PSP1(YDR505C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:RAD9(YDR217C)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RDS1(YCR106W)|FD-Score:7.29|P-value:1.50E-13||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RIM8(YGL045W)|FD-Score:-4.01|P-value:3.05E-5||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RLF2(YPR018W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL23B(YER117W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RSM7(YJR113C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTK1(YDL025C)|FD-Score:-4.93|P-value:4.21E-7||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:RUB1(YDR139C)|FD-Score:-3.92|P-value:4.52E-5||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SDH2(YLL041C)|FD-Score:-3.13|P-value:8.81E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SDT1(YGL224C)|FD-Score:3.83|P-value:6.32E-5||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SHE3(YBR130C)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance Gene:SNO1(YMR095C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SVF1(YDR346C)|FD-Score:-3.23|P-value:6.11E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TDH2(YJR009C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:VIK1(YPL253C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:VMA5(YKL080W)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:YCL042W(YCL042W_p)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR064C(YCR064C_d)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YEA6(YEL006W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGL039W(YGL039W)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols Gene:YGL177W(YGL177W_d)|FD-Score:7.1|P-value:6.34E-13||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR078W(YHR078W_p)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YHR131C(YHR131C_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YJR079W(YJR079W_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YLL007C(YLL007C_p)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR428C(YLR428C_d)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1 Gene:YNL122C(YNL122C_p)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YPL277C(YPL277C_p)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR150W(YPR150W_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL058W8.662.33E-181.57USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YMR208W7.522.77E-141.57ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR060W6.641.58E-111.57MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YDR362C5.071.98E-70.76TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YOL094C4.318.29E-60.06RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR294W4.251.08E-50.07RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YOR278W4.181.46E-50.24HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YER003C3.944.07E-50.03PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YNL317W3.914.61E-50.10PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YGR156W3.817.03E-50.01PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YLR249W3.797.39E-50.04YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YBL018C3.758.67E-50.09POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL169C3.661.25E-40.22MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YIL062C3.442.89E-40.19ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YNL178W3.255.82E-40.07RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR119W8.102.66E-16ASI1Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
YCR106W7.291.50E-13RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YGL177W_d7.106.34E-13YGL177W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR251W5.422.94E-8PAM1Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype
YKL007W4.856.20E-7CAP1Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YBR179C4.721.16E-6FZO1Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
YGL023C4.376.26E-6PIB2Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
YEL006W4.131.80E-5YEA6Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog
YBR151W4.121.90E-5APD1Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
YBR130C4.052.54E-5SHE3Protein that acts as an adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance
YOL164W3.973.62E-5BDS1Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources
YKL139W3.914.62E-5CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YJL099W3.875.44E-5CHS6Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
YGL224C3.836.32E-5SDT1Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives
YKL080W3.826.60E-5VMA5Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane

GO enrichment analysis for SGTC_633
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2313.64E-72SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.09375DNA intercalators
0.1995.57E-54SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.0808081amide catabolism
0.1952.33E-51SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.16092DNA intercalators
0.1925.64E-50SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.146341DNA intercalators
0.1842.55E-46SGTC_1459k035-0031 35.7 μMChemDiv (Drug-like library)38771280.0632911DNA intercalators
0.1822.37E-45SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.121951DNA intercalators
0.1721.97E-40SGTC_512prima-1 270.0 μMICCB bioactive library3229680.0379747DNA intercalators
0.1713.55E-40SGTC_469w7 172.0 μMICCB bioactive library451155700.0833333ERG2
0.1599.46E-35SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.134146amide catabolism
0.1532.67E-32SGTC_12900986-0249 10.2 μMChemDiv (Drug-like library)533290.189189DNA intercalators
0.1372.83E-26SGTC_711k292-0785 179.0 μMChemDiv (Drug-like library)41463150.0804598DNA intercalators
0.1352.16E-25SGTC_470aa-861 153.0 μMICCB bioactive library19670.047619sphingolipid biosynthesis & PDR1
0.1292.10E-23SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.150685DNA intercalators
0.1293.55E-23SGTC_1250133-0007 11.8 μMChemDiv (Drug-like library)265410.0714286DNA intercalators
0.1232.95E-21SGTC_1465k213-0056 144.0 μMChemDiv (Drug-like library)240488570.146067DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6520437-0092114 μM0.7058823091274ChemDiv (Drug-like library)507.775385.60636
SGTC_13281441-002569.2 μM0.3571434562601ChemDiv (Drug-like library)287.270722.2724
SGTC_2831799903619.48 μM0.3513512988270Chembridge (Drug-like library)323.309654.03115
SGTC_8890919-152482.7 μM0.3378383905452ChemDiv (Drug-like library)274.271962.85825
SGTC_1967st07017751.4 μM0.3292685729214TimTec (Natural product derivative library)389.400665.14705
SGTC_1778st04681958.9 μM0.325671715TimTec (Natural product derivative library)339.345283.69324
SGTC_850868-0259160.06 μM0.32352983173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_458nppb133 μM0.3157894549ICCB bioactive library300.309243.52325
SGTC_1880MG-132525 μM0.311688462382Miscellaneous475.620844.37135
SGTC_520329-0193875 nM0.3055563739292ChemDiv (Drug-like library)325.575642.80814TSC3-RPN4