1611-4317

N-[2,2,2-trichloro-1-(4-iodoanilino)ethyl]thiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_634
Screen concentration 11.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 2843522
SMILES C1=CSC(=C1)C(=O)NC(C(Cl)(Cl)Cl)NC2=CC=C(C=C2)I
Standardized SMILES ClC(Cl)(Cl)C(NC(=O)c1cccs1)Nc2ccc(I)cc2
Molecular weight 475.5598
ALogP 4.82
H-bond donor count 2
H-bond acceptor count 3
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.96
% growth inhibition (Hom. pool) 7.82


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2843522
Download HIP data (tab-delimited text)  (excel)
Gene:GLE1(YDL207W)|FD-Score:3.89|P-value:4.99E-5|Clearance:0.03||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM2(YGL040C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.02||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:IPI3(YNL182C)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.02||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:RFC4(YOL094C)|FD-Score:4.53|P-value:2.98E-6|Clearance:0.46||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIM2(YBR192W)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.09||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RMP1(YLR145W)|FD-Score:5.29|P-value:6.23E-8|Clearance:0.46||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPC34(YNR003C)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.46||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RPN3(YER021W)|FD-Score:5.54|P-value:1.54E-8|Clearance:0.46||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN8(YOR261C)|FD-Score:4.06|P-value:2.49E-5|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPT5(YOR117W)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.16||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:3.85|P-value:5.99E-5|Clearance:0.41||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SRP1(YNL189W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.11||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TFA1(YKL028W)|FD-Score:4.86|P-value:5.87E-7|Clearance:0.46||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TOM40(YMR203W)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.01||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC11(YER093C)|FD-Score:5.02|P-value:2.56E-7|Clearance:0.46||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:GLE1(YDL207W)|FD-Score:3.89|P-value:4.99E-5|Clearance:0.03||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:HEM2(YGL040C)|FD-Score:3.28|P-value:5.15E-4|Clearance:0.02||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:IPI3(YNL182C)|FD-Score:4.07|P-value:2.32E-5|Clearance:0.02||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:RFC4(YOL094C)|FD-Score:4.53|P-value:2.98E-6|Clearance:0.46||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIM2(YBR192W)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.09||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RMP1(YLR145W)|FD-Score:5.29|P-value:6.23E-8|Clearance:0.46||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPC34(YNR003C)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.46||SGD DESC:RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex Gene:RPN3(YER021W)|FD-Score:5.54|P-value:1.54E-8|Clearance:0.46||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN8(YOR261C)|FD-Score:4.06|P-value:2.49E-5|Clearance:0.01||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPT5(YOR117W)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.16||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:3.85|P-value:5.99E-5|Clearance:0.41||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SRP1(YNL189W)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.11||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TFA1(YKL028W)|FD-Score:4.86|P-value:5.87E-7|Clearance:0.46||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TOM40(YMR203W)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.01||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TSC11(YER093C)|FD-Score:5.02|P-value:2.56E-7|Clearance:0.46||SGD DESC:Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.97|P-value:3.62E-5|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2843522
Download HOP data (tab-delimited text)  (excel)
Gene:APE3(YBR286W)|FD-Score:5.84|P-value:2.62E-9||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:BIM1(YER016W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BPT1(YLL015W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p Gene:CHO1(YER026C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CMK2(YOL016C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:DAS1(YJL149W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DDP1(YOR163W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DJP1(YIR004W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:ERG2(YMR202W)|FD-Score:18.3|P-value:6.07E-75||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG5(YMR015C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FSH2(YMR222C)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:FUN26(YAL022C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GAL11(YOL051W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDT1(YBR187W_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GET3(YDL100C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRE2(YOL151W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HEF3(YNL014W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HFI1(YPL254W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOR7(YMR251W-A)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HTA2(YBL003C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IDH2(YOR136W)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:LEU1(YGL009C)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:MCH5(YOR306C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MID1(YNL291C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:NCL1(YBL024W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:PAU17(YLL025W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PDR8(YLR266C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:6.59|P-value:2.26E-11||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PPM2(YOL141W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:REV1(YOR346W)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL24A(YGL031C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS4A(YJR145C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT16(YNL105W_d)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSM28(YDR494W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SDH2(YLL041C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SDT1(YGL224C)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SFB3(YHR098C)|FD-Score:7.4|P-value:6.57E-14||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGE1(YPR198W)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKI8(YGL213C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SPT3(YDR392W)|FD-Score:7.66|P-value:9.10E-15||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSN2(YDR443C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TAN1(YGL232W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TIM18(YOR297C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TPK2(YPL203W)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPO1(YLL028W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:YDR401W(YDR401W_d)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL023C(YEL023C_p)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YGL041C(YGL041C_d)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM2(YMR151W_d)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YKL053W(YKL053W_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR280C(YLR280C_d)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR010W(YMR010W_p)|FD-Score:7.65|P-value:1.02E-14||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YOR316C-A(YOR316C-A_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR345C(YOR345C_d)|FD-Score:4.37|P-value:6.16E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPK1(YKL126W)|FD-Score:13.5|P-value:8.16E-42||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL277C(YPL277C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:YPR039W(YPR039W_d)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR127W(YPR127W)|FD-Score:-5.06|P-value:2.08E-7||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:APE3(YBR286W)|FD-Score:5.84|P-value:2.62E-9||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:BIM1(YER016W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BPT1(YLL015W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p Gene:CHO1(YER026C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CMK2(YOL016C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:DAS1(YJL149W)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DDP1(YOR163W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DJP1(YIR004W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:ERG2(YMR202W)|FD-Score:18.3|P-value:6.07E-75||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG5(YMR015C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FSH2(YMR222C)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:FUN26(YAL022C)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GAL11(YOL051W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDT1(YBR187W_p)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis Gene:GET3(YDL100C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GRE2(YOL151W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HEF3(YNL014W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HFI1(YPL254W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOR7(YMR251W-A)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HTA2(YBL003C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IDH2(YOR136W)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:LEU1(YGL009C)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:MCH5(YOR306C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MID1(YNL291C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:NCL1(YBL024W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:PAU17(YLL025W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PDR8(YLR266C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:6.59|P-value:2.26E-11||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PPM2(YOL141W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:REV1(YOR346W)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL24A(YGL031C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Ribosomal 60S subunit protein L24A; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS4A(YJR145C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT16(YNL105W_d)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSM28(YDR494W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SDH2(YLL041C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SDT1(YGL224C)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SFB3(YHR098C)|FD-Score:7.4|P-value:6.57E-14||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SGE1(YPR198W)|FD-Score:3.8|P-value:7.12E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SKI8(YGL213C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SPT3(YDR392W)|FD-Score:7.66|P-value:9.10E-15||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SSN2(YDR443C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:TAN1(YGL232W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TIM18(YOR297C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TPK2(YPL203W)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPO1(YLL028W)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:YDR401W(YDR401W_d)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL023C(YEL023C_p)|FD-Score:-3.15|P-value:8.13E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YGL041C(YGL041C_d)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM2(YMR151W_d)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YKL053W(YKL053W_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR280C(YLR280C_d)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR010W(YMR010W_p)|FD-Score:7.65|P-value:1.02E-14||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YOR316C-A(YOR316C-A_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR345C(YOR345C_d)|FD-Score:4.37|P-value:6.16E-6||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPK1(YKL126W)|FD-Score:13.5|P-value:8.16E-42||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL277C(YPL277C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels Gene:YPR039W(YPR039W_d)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR127W(YPR127W)|FD-Score:-5.06|P-value:2.08E-7||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER021W5.541.54E-80.46RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YLR145W5.296.23E-80.46RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
YER093C5.022.56E-70.46TSC11Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
YKL028W4.865.87E-70.46TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YNR003C4.661.55E-60.46RPC34RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
YOL094C4.532.98E-60.46RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YNL182C4.072.32E-50.02IPI3Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene
YOR261C4.062.49E-50.01RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YLR033W4.052.59E-50.08RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YMR290W-A_d3.973.62E-50.08YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YDL207W3.894.99E-50.03GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YMR203W3.865.71E-50.01TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YGL048C3.855.99E-50.41RPT6ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
YOR117W3.442.91E-40.16RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YGL040C3.285.15E-40.02HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W18.306.07E-75ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YKL126W13.508.16E-42YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDR392W7.669.10E-15SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YMR010W_p7.651.02E-14YMR010W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1
YHR098C7.406.57E-14SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YGL025C6.592.26E-11PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YBR286W5.842.62E-9APE3Vacuolar aminopeptidase Y, processed to mature form by Prb1p
YOR346W4.963.51E-7REV1Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YPL203W4.651.64E-6TPK2cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase
YOR345C_d4.376.16E-6YOR345C_dDubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YMR251W-A4.161.62E-5HOR7Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
YLR280C_d4.141.76E-5YLR280C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER016W4.062.43E-5BIM1Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
YOR316C-A_p4.022.89E-5YOR316C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YNL291C3.944.01E-5MID1N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer

GO enrichment analysis for SGTC_634
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3611.49E-181SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.08ERG2
0.3373.99E-157SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0632911ERG2
0.3096.58E-131SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.121951ERG2
0.2994.19E-122SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.109375ERG2
0.2881.80E-113SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.152778ERG2
0.2842.65E-110SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.115942ERG2
0.2702.45E-99SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.164384ERG2
0.2695.75E-98SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.0731707ERG2
0.2593.33E-91SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0545455ERG2
0.2581.99E-90SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.0843373ERG2
0.2423.61E-79SGTC_7764476-4378 112.0 μMChemDiv (Drug-like library)28920080.0886076ERG2
0.2343.10E-74SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.091954
0.2341.60E-74SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.0617284ERG2
0.2243.69E-68SGTC_2732terfenadine 20.0 μMMiscellaneous54050.0769231ERG2
0.2249.67E-68SGTC_5181021-0119 62.6 μMChemDiv (Drug-like library)10977620.0897436ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3071501-122322.3 μM0.4444442830329ChemDiv (Drug-like library)412.49374.43623
SGTC_7891309-113570.6 μM0.4363643092424ChemDiv (Drug-like library)379.068643.80623ERG2
SGTC_9831491-082112.2 μM0.43396246495113ChemDiv (Drug-like library)440.5220435.24623
SGTC_2061524588480.95 μM0.352941573863Chembridge (Fragment library)210.276041.56514tubulin folding & SWR complex
SGTC_22647948757110.76 μM0.3396232971411Chembridge (Fragment library)252.312721.35624
SGTC_6293448-646676.7 μM0.333333573744ChemDiv (Drug-like library)254.3072.75413
SGTC_9143820-6779408 μM0.3333332893481ChemDiv (Drug-like library)392.450884.69725
SGTC_20535265679138 μM0.333333764595Chembridge (Fragment library)233.286242.55113
SGTC_2276794751342.08 μM0.3333332105100Chembridge (Fragment library)234.297442.23314
SGTC_3187910939449.47 μM0.32758617098090Chembridge (Drug-like library)247.312822.913Golgi