3381-0960

9-ethyl-3-[[4-[(3,4,5-trimethoxyphenyl)methyl]piperazin-1-yl]methyl]carbazole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_640
Screen concentration 37.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 1745025
SMILES CCN1C2=C(C=C(C=C2)CN3CCN(CC3)CC4=CC(=C(C(=C4)OC)OC)OC)C5=CC=CC=C51
Standardized SMILES CCn1c2ccccc2c3cc(CN4CCN(Cc5cc(OC)c(OC)c(OC)c5)CC4)ccc13
Molecular weight 473.6065
ALogP 5.44
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 23.76
% growth inhibition (Hom. pool) 3.46


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1745025
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:4.02|P-value:2.93E-5|Clearance:0.06||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:AUR1(YKL004W)|FD-Score:5.43|P-value:2.78E-8|Clearance:0.81||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:CCT6(YDR188W)|FD-Score:3.92|P-value:4.37E-5|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:ESP1(YGR098C)|FD-Score:-5.06|P-value:2.15E-7|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FOL3(YMR113W)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.04||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:KRE33(YNL132W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.13||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LSG1(YGL099W)|FD-Score:3.87|P-value:5.36E-5|Clearance:0.06||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.03||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:4.62|P-value:1.91E-6|Clearance:0.27||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPB7(YDR404C)|FD-Score:5.62|P-value:9.59E-9|Clearance:0.81||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPP1(YHR062C)|FD-Score:4.35|P-value:6.90E-6|Clearance:0.31||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN9(YMR270C)|FD-Score:-3.78|P-value:7.91E-5|Clearance:0||SGD DESC:Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I Gene:SPC42(YKL042W)|FD-Score:4.03|P-value:2.76E-5|Clearance:0.01||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TCP1(YDR212W)|FD-Score:3.81|P-value:6.88E-5|Clearance:0.59||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TIF6(YPR016C)|FD-Score:3.9|P-value:4.74E-5|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:ACS2(YLR153C)|FD-Score:4.02|P-value:2.93E-5|Clearance:0.06||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:AUR1(YKL004W)|FD-Score:5.43|P-value:2.78E-8|Clearance:0.81||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:CCT6(YDR188W)|FD-Score:3.92|P-value:4.37E-5|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:ESP1(YGR098C)|FD-Score:-5.06|P-value:2.15E-7|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FOL3(YMR113W)|FD-Score:3.09|P-value:9.91E-4|Clearance:0.04||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:KRE33(YNL132W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.13||SGD DESC:Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:LSG1(YGL099W)|FD-Score:3.87|P-value:5.36E-5|Clearance:0.06||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:3.96|P-value:3.80E-5|Clearance:0.03||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP2(YNL061W)|FD-Score:4.62|P-value:1.91E-6|Clearance:0.27||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPB7(YDR404C)|FD-Score:5.62|P-value:9.59E-9|Clearance:0.81||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPP1(YHR062C)|FD-Score:4.35|P-value:6.90E-6|Clearance:0.31||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN9(YMR270C)|FD-Score:-3.78|P-value:7.91E-5|Clearance:0||SGD DESC:Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I Gene:SPC42(YKL042W)|FD-Score:4.03|P-value:2.76E-5|Clearance:0.01||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TCP1(YDR212W)|FD-Score:3.81|P-value:6.88E-5|Clearance:0.59||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TIF6(YPR016C)|FD-Score:3.9|P-value:4.74E-5|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1745025
Download HOP data (tab-delimited text)  (excel)
Gene:AIM5(YBR262C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD6(YPL061W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AQY2(YLL052C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:BDH2(YAL061W_p)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:CTS1(YLR286C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:CWH43(YCR017C)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DIA3(YDL024C)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:EMC4(YGL231C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:GYL1(YMR192W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HAP3(YBL021C)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HPA3(YEL066W)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:LST4(YKL176C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MDY2(YOL111C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MMT1(YMR177W)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MUP3(YHL036W)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:PAN2(YGL094C)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEP3(YLR148W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PHO90(YJL198W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:RAM1(YDL090C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RPL19B(YBL027W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RUB1(YDR139C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SDS23(YGL056C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SGT2(YOR007C)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SLM4(YBR077C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SOK1(YDR006C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SOL4(YGR248W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TGL4(YKR089C)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:THI3(YDL080C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:UBC7(YMR022W)|FD-Score:4.36|P-value:6.51E-6||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VPS3(YDR495C)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL094C(YBL094C_d)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Putative protein of unknown function Gene:YCR090C(YCR090C_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function Gene:YDL180W(YDL180W_p)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR306C(YDR306C_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR401W(YDR401W_d)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YJL215C(YJL215C_d)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR010W(YMR010W_p)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR252C(YMR252C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YTP1(YNL237W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AIM5(YBR262C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD6(YPL061W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AQY2(YLL052C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:BDH2(YAL061W_p)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:CTS1(YLR286C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:CWH43(YCR017C)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DIA3(YDL024C)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:EMC4(YGL231C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:GYL1(YMR192W)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HAP3(YBL021C)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HPA3(YEL066W)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:LST4(YKL176C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MDY2(YOL111C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MMT1(YMR177W)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p Gene:MUP3(YHL036W)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:PAN2(YGL094C)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEP3(YLR148W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PHO90(YJL198W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:RAM1(YDL090C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RPL19B(YBL027W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RUB1(YDR139C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) Gene:SDS23(YGL056C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SGT2(YOR007C)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SLM4(YBR077C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SOK1(YDR006C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SOL4(YGR248W)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TGL4(YKR089C)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p Gene:THI3(YDL080C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:UBC7(YMR022W)|FD-Score:4.36|P-value:6.51E-6||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VPS3(YDR495C)|FD-Score:3.78|P-value:7.93E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL094C(YBL094C_d)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YCL021W-A(YCL021W-A_p)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Putative protein of unknown function Gene:YCR090C(YCR090C_p)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function Gene:YDL180W(YDL180W_p)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR306C(YDR306C_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR401W(YDR401W_d)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL042C(YFL042C_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YJL215C(YJL215C_d)|FD-Score:-3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR010W(YMR010W_p)|FD-Score:-3.89|P-value:5.07E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR252C(YMR252C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YTP1(YNL237W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR404C5.629.59E-90.81RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YKL004W5.432.78E-80.81AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
YNL061W4.621.91E-60.27NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YHR062C4.356.90E-60.32RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL042W4.032.76E-50.01SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YLR153C4.022.93E-50.06ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YHR170W3.963.80E-50.03NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDR188W3.924.37E-50.02CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YPR016C3.904.74E-50.03TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YGL099W3.875.36E-50.06LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR212W3.816.88E-50.59TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YNL132W3.226.31E-40.13KRE33Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YMR113W3.099.91E-40.04FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YKR083C3.050.001130.12DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YGR245C2.940.001650.06SDA1Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR017C4.827.02E-7CWH43Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
YDR139C4.741.09E-6RUB1Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
YDR293C4.731.14E-6SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YGL231C4.641.77E-6EMC4Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85
YOR007C4.395.64E-6SGT2Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
YMR022W4.366.51E-6UBC7Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
YBL021C4.231.17E-5HAP3Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
YKR089C4.032.80E-5TGL4Multifunctional triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p
YDL024C4.022.87E-5DIA3Protein of unknown function, involved in invasive and pseudohyphal growth
YBR262C4.022.88E-5AIM5Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YMR242C3.993.30E-5RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YMR177W3.963.68E-5MMT1Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt2p
YBR077C3.944.15E-5SLM4Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
YGL094C3.934.21E-5PAN2Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YDR495C3.787.93E-5VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase

GO enrichment analysis for SGTC_640
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1172.26E-19SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.122222
0.1141.28E-18SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.13636460S ribosome export
0.1141.46E-18SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.10256460S ribosome export
0.1041.08E-15SGTC_1360097-0015 404.5 μMChemDiv (Drug-like library)48613370.073529460S ribosome export
0.1031.79E-15SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.104651
0.1032.57E-15SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.11956560S ribosome export
0.0983.53E-14SGTC_5700987-0065 62.6 μMChemDiv (Drug-like library)31044280.078947460S ribosome export
0.0976.40E-14SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0657895
0.0921.73E-12SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.28205160S ribosome export
0.0912.54E-12SGTC_6470140-0236 81.0 μMChemDiv (Drug-like library)9908370.152542
0.0904.67E-12SGTC_1875nocodazole 6.0 μMMiscellaneous41220.0963855tubulin folding & SWR complex
0.0896.01E-12SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.077922160S ribosome export
0.0881.64E-11SGTC_31709103054 49.5 μMChembridge (Drug-like library)164329700.160S ribosome export
0.0872.20E-11SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0681818PDR1
0.0863.52E-11SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.11627960S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12560570-0116285 μM0.510204410638ChemDiv (Drug-like library)280.362661.81105
SGTC_10094106-00579.87 μM0.4218752928005ChemDiv (Drug-like library)443.580525.0314
SGTC_7924534-1957155 μM0.3934431717904ChemDiv (Drug-like library)282.337022.73412
SGTC_11584092-049491.8 μM0.3906251148199ChemDiv (Drug-like library)416.512124.2704
SGTC_14414106-004013 μM0.3870972904180ChemDiv (Drug-like library)433.973025.36213
SGTC_1784092-080687.51 μM0.3333332169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_5423381-024489.3 μM0.3333331112843ChemDiv (Drug-like library)360.448863.67603
SGTC_2790542397071.43 μM0.3134331376240Chembridge (Drug-like library)389.486785.09604endomembrane recycling
SGTC_13752910-0797186 μM0.307692742508ChemDiv (Drug-like library)340.416162.75204
SGTC_14354092-078956.7 μM0.3064522248285ChemDiv (Drug-like library)344.87834.44103plasma membrane duress