4092-0595

1-[(4-methylsulfanylphenyl)methyl]-4-methylsulfonylpiperazine

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_641
Screen concentration 30.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 786260
SMILES CSC1=CC=C(C=C1)CN2CCN(CC2)S(=O)(=O)C
Standardized SMILES CSc1ccc(CN2CCN(CC2)S(=O)(=O)C)cc1
Molecular weight 300.4401
ALogP 1.45
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.75
% growth inhibition (Hom. pool) 4.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 786260
Download HIP data (tab-delimited text)  (excel)
Gene:BRX1(YOL077C)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.04||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:DML1(YMR211W)|FD-Score:4.65|P-value:1.68E-6|Clearance:0.15||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:6.81|P-value:4.80E-12|Clearance:1.31||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.20E-5|Clearance:0.12||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IPL1(YPL209C)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.04||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:NOP2(YNL061W)|FD-Score:4.5|P-value:3.41E-6|Clearance:0.04||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP58(YOR310C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.26||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:OLE1(YGL055W)|FD-Score:6.52|P-value:3.42E-11|Clearance:1.31||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:ORC3(YLL004W)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PDS1(YDR113C)|FD-Score:4.29|P-value:8.95E-6|Clearance:0.04||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP4(YPR178W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.26||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PXR1(YGR280C)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.03||SGD DESC:Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain Gene:QNS1(YHR074W)|FD-Score:3.72|P-value:9.88E-5|Clearance:0.03||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RHO1(YPR165W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.01||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL25(YOL127W)|FD-Score:4.45|P-value:4.21E-6|Clearance:0.16||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL30(YGL030W)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:-3.56|P-value:1.85E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SNM1(YDR478W)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.1||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SUP45(YBR143C)|FD-Score:5.96|P-value:1.29E-9|Clearance:1.31||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TFA2(YKR062W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.03||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UBC1(YDR177W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP11(YKL099C)|FD-Score:-3.09|P-value:9.91E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:BRX1(YOL077C)|FD-Score:3.7|P-value:1.10E-4|Clearance:0.04||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:DML1(YMR211W)|FD-Score:4.65|P-value:1.68E-6|Clearance:0.15||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG11(YHR007C)|FD-Score:6.81|P-value:4.80E-12|Clearance:1.31||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GLC7(YER133W)|FD-Score:-3.12|P-value:9.04E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.20E-5|Clearance:0.12||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IPL1(YPL209C)|FD-Score:3.11|P-value:9.32E-4|Clearance:0.04||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:NOP2(YNL061W)|FD-Score:4.5|P-value:3.41E-6|Clearance:0.04||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP58(YOR310C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.26||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:OLE1(YGL055W)|FD-Score:6.52|P-value:3.42E-11|Clearance:1.31||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:ORC3(YLL004W)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.01||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PDS1(YDR113C)|FD-Score:4.29|P-value:8.95E-6|Clearance:0.04||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRP4(YPR178W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.26||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PXR1(YGR280C)|FD-Score:3.37|P-value:3.79E-4|Clearance:0.03||SGD DESC:Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain Gene:QNS1(YHR074W)|FD-Score:3.72|P-value:9.88E-5|Clearance:0.03||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RHO1(YPR165W)|FD-Score:3.24|P-value:6.07E-4|Clearance:0.01||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL25(YOL127W)|FD-Score:4.45|P-value:4.21E-6|Clearance:0.16||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL30(YGL030W)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.15||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RPP1(YHR062C)|FD-Score:-3.56|P-value:1.85E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SNM1(YDR478W)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.1||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SUP45(YBR143C)|FD-Score:5.96|P-value:1.29E-9|Clearance:1.31||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TFA2(YKR062W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.03||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UBC1(YDR177W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP11(YKL099C)|FD-Score:-3.09|P-value:9.91E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 786260
Download HOP data (tab-delimited text)  (excel)
Gene:ARP5(YNL059C)|FD-Score:4.77|P-value:9.34E-7||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ART5(YGR068C)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:CNM67(YNL225C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COA1(YIL157C)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:CYS4(YGR155W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAS1(YJL149W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DNF1(YER166W)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ERP4(YOR016C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR10(YLR238W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FMP37(YGL080W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GLG2(YJL137C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HAL9(YOL089C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HNT1(YDL125C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HPC2(YBR215W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:IES2(YNL215W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:KAR4(YCL055W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:MRC1(YCL061C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRP4(YHL004W)|FD-Score:3.14|P-value:8.30E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NPP1(YCR026C)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OPI7(YDR360W_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PAH1(YMR165C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PCI8(YIL071C)|FD-Score:7.82|P-value:2.65E-15||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEX28(YHR150W)|FD-Score:4.84|P-value:6.40E-7||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PEX4(YGR133W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:QCR9(YGR183C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RCY1(YJL204C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO2(YNL090W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPS0A(YGR214W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SIL1(YOL031C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIT4(YDL047W)|FD-Score:5.52|P-value:1.66E-8||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SPO7(YAL009W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSN2(YDR443C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUR2(YDR297W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TMA64(YDR117C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TOM1(YDR457W)|FD-Score:11.1|P-value:8.26E-29||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOS1(YBR162C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRI1(YMR233W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBC13(YDR092W)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:VIP1(YLR410W)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VOA1(YGR106C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS9(YML097C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR174C(YBR174C_d)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR025C(YCR025C_d)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR219W(YGR219W_d)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL005C(YHL005C_d)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YIL029C(YIL029C_p)|FD-Score:6.37|P-value:9.75E-11||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YMR209C(YMR209C_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YOP1(YPR028W)|FD-Score:4.34|P-value:7.11E-6||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPK1(YKL126W)|FD-Score:5.45|P-value:2.46E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL136W(YPL136W_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:ARP5(YNL059C)|FD-Score:4.77|P-value:9.34E-7||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ART5(YGR068C)|FD-Score:3.12|P-value:8.95E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:CNM67(YNL225C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COA1(YIL157C)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:CYS4(YGR155W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAS1(YJL149W)|FD-Score:-3.26|P-value:5.58E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DNF1(YER166W)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:ERP4(YOR016C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FAR10(YLR238W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:FMP37(YGL080W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GLG2(YJL137C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HAL9(YOL089C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HNT1(YDL125C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HPC2(YBR215W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:IES2(YNL215W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:KAR4(YCL055W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:MRC1(YCL061C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRP4(YHL004W)|FD-Score:3.14|P-value:8.30E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NPP1(YCR026C)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OPI7(YDR360W_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PAH1(YMR165C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PCI8(YIL071C)|FD-Score:7.82|P-value:2.65E-15||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEX28(YHR150W)|FD-Score:4.84|P-value:6.40E-7||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PEX4(YGR133W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:QCR9(YGR183C)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RCY1(YJL204C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RHO2(YNL090W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPS0A(YGR214W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SIL1(YOL031C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIT4(YDL047W)|FD-Score:5.52|P-value:1.66E-8||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SPO7(YAL009W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSN2(YDR443C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUR2(YDR297W)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TMA64(YDR117C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TOM1(YDR457W)|FD-Score:11.1|P-value:8.26E-29||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOS1(YBR162C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRI1(YMR233W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBC13(YDR092W)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:VIP1(YLR410W)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VOA1(YGR106C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS9(YML097C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:YBR174C(YBR174C_d)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCR025C(YCR025C_d)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR219W(YGR219W_d)|FD-Score:3.14|P-value:8.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C Gene:YHL005C(YHL005C_d)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W Gene:YIL029C(YIL029C_p)|FD-Score:6.37|P-value:9.75E-11||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YMR209C(YMR209C_p)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YOP1(YPR028W)|FD-Score:4.34|P-value:7.11E-6||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YPK1(YKL126W)|FD-Score:5.45|P-value:2.46E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL136W(YPL136W_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR007C6.814.80E-121.31ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YGL055W6.523.42E-111.31OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YBR143C5.961.29E-91.31SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YMR211W4.651.68E-60.15DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YNL061W4.503.41E-60.04NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YOL127W4.454.21E-60.17RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YDR113C4.298.95E-60.04PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YGL030W4.251.09E-50.15RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YPR178W4.102.11E-50.26PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YDR047W3.846.20E-50.12HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YHR074W3.729.88E-50.03QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YOL077C3.701.10E-40.04BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YKR062W3.661.27E-40.03TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YOR310C3.631.41E-40.26NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YGR280C3.373.79E-40.03PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR457W11.108.26E-29TOM1E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
YIL071C7.822.65E-15PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YIL029C_p6.379.75E-11YIL029C_pPutative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W
YDL047W5.521.66E-8SIT4Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization
YKL126W5.452.46E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YCR026C4.953.75E-7NPP1Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication
YHR150W4.846.40E-7PEX28Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p
YNL059C4.779.34E-7ARP5Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YPR028W4.347.11E-6YOP1Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
YLR425W4.289.26E-6TUS1Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
YOL089C4.151.68E-5HAL9Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
YDR443C3.983.44E-5SSN2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
YGR155W3.865.78E-5CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YER166W3.778.23E-5DNF1Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
YHL005C_d3.768.53E-5YHL005C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W

GO enrichment analysis for SGTC_641
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1284.20E-23SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.155172fatty acid desaturase (OLE1)
0.1211.42E-20SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.0634921
0.1164.25E-19SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.138462endomembrane recycling
0.1094.26E-17SGTC_23899091542 195.5 μMChembridge (Fragment library)36266240.101695iron homeostasis
0.1094.82E-17SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.326087fatty acid desaturase (OLE1)
0.0979.65E-14SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.147541fatty acid desaturase (OLE1)
0.0943.73E-13SGTC_258haloperidol 50.8 μMMiscellaneous35590.134328fatty acid desaturase (OLE1)
0.0912.06E-12SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.0655738
0.0912.08E-12SGTC_13081187-1617 41.4 μMChemDiv (Drug-like library)28825700.129032fatty acid desaturase (OLE1)
0.0912.34E-12SGTC_11604092-0570 10.5 μMChemDiv (Drug-like library)49949750.461538plasma membrane duress
0.0913.38E-12SGTC_1811447-1644 8.1 μMChemDiv (Drug-like library)5346000.0945946endomembrane recycling
0.0895.77E-12SGTC_659k018-0002 107.0 μMChemDiv (Drug-like library)67978790.0609756
0.0871.80E-11SGTC_224benfluorex 43.9 μMMiscellaneous23180.0985916
0.0872.17E-11SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.057971RSC & ERG11
0.0872.51E-11SGTC_9511171-0579 82.7 μMChemDiv (Drug-like library)16160500.0746269cell wall

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_11604092-057010.5 μM0.4615384994975ChemDiv (Drug-like library)390.926823.82604plasma membrane duress
SGTC_14554262-0120131 μM0.41529587ChemDiv (Drug-like library)308.460424.87302plasma membrane duress
SGTC_2784092-082123.9 μM0.390244877900ChemDiv (Drug-like library)328.87895.05102plasma membrane duress
SGTC_7064262-005746.9 μM0.363636883298ChemDiv (Drug-like library)338.48644.71903fatty acid desaturase (OLE1)
SGTC_20955432005200 μM0.357143783734Chembridge (Fragment library)204.31132.52602
SGTC_1784092-080687.51 μM0.3404262169044ChemDiv (Drug-like library)328.87895.05102ergosterol depletion effects on membrane
SGTC_6164092-0450108 μM0.34787165ChemDiv (Drug-like library)322.443943.76202
SGTC_2014262-015912.8 μM0.3333331341029ChemDiv (Drug-like library)300.481484.91302ergosterol depletion effects on membrane
SGTC_5154092-1064102 μM0.333333717353ChemDiv (Drug-like library)335.27084.77302plasma membrane duress
SGTC_2887904478458.44 μM0.3269236465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)