0845-0943

N-[3-chloro-4-(4-chlorophenoxy)phenyl]-6-methoxy-2-methylquinolin-4-amine

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_645
Screen concentration 5.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 1885106
SMILES CC1=NC2=C(C=C(C=C2)OC)C(=C1)NC3=CC(=C(C=C3)OC4=CC=C(C=C4)Cl)Cl
Standardized SMILES COc1ccc2nc(C)cc(Nc3ccc(Oc4ccc(Cl)cc4)c(Cl)c3)c2c1
Molecular weight 425.3072
ALogP 6.72
H-bond donor count 1
H-bond acceptor count 4
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.85
% growth inhibition (Hom. pool) 8.34


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1885106
Download HIP data (tab-delimited text)  (excel)
Gene:BCP1(YDR361C)|FD-Score:-3.12|P-value:9.00E-4|Clearance:0||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:BET3(YKR068C)|FD-Score:-3.31|P-value:4.69E-4|Clearance:0||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:GPI16(YHR188C)|FD-Score:3.24|P-value:5.90E-4|Clearance:0.02||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:LSG1(YGL099W)|FD-Score:9.26|P-value:1.02E-20|Clearance:3.14||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:6.12|P-value:4.81E-10|Clearance:1.53||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUT2(YPR168W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.76||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:POB3(YML069W)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.39||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:RPL32(YBL092W)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS3(YNL178W)|FD-Score:-3.27|P-value:5.47E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SOF1(YLL011W)|FD-Score:3.34|P-value:4.12E-4|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPT14(YPL175W)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.1||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:UTP6(YDR449C)|FD-Score:-3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VTI1(YMR197C)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.06||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJR012C(YJR012C_p)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.03||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YNL150W(YNL150W_d)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:BCP1(YDR361C)|FD-Score:-3.12|P-value:9.00E-4|Clearance:0||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:BET3(YKR068C)|FD-Score:-3.31|P-value:4.69E-4|Clearance:0||SGD DESC:Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex Gene:GPI16(YHR188C)|FD-Score:3.24|P-value:5.90E-4|Clearance:0.02||SGD DESC:Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog Gene:LSG1(YGL099W)|FD-Score:9.26|P-value:1.02E-20|Clearance:3.14||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:6.12|P-value:4.81E-10|Clearance:1.53||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUT2(YPR168W)|FD-Score:4.17|P-value:1.54E-5|Clearance:0.76||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:POB3(YML069W)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.39||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:RPL32(YBL092W)|FD-Score:-3.37|P-value:3.79E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS3(YNL178W)|FD-Score:-3.27|P-value:5.47E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SOF1(YLL011W)|FD-Score:3.34|P-value:4.12E-4|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPT14(YPL175W)|FD-Score:3.34|P-value:4.19E-4|Clearance:0.1||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:UTP6(YDR449C)|FD-Score:-3.25|P-value:5.67E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:VTI1(YMR197C)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.06||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YJR012C(YJR012C_p)|FD-Score:4.58|P-value:2.28E-6|Clearance:0.03||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YNL150W(YNL150W_d)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1885106
Download HOP data (tab-delimited text)  (excel)
Gene:AQR1(YNL065W)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:CCP1(YKR066C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:DAP2(YHR028C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DCN1(YLR128W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DIG1(YPL049C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DST1(YGL043W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM1(YAL059W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm Gene:FLC1(YPL221W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP10(YER182W_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HAL5(YJL165C)|FD-Score:4.76|P-value:9.77E-7||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:HLR1(YDR528W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; <br>HLR1 has a paralog, LRE1, that arose from the whole genome duplication Gene:JJJ1(YNL227C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:MNN2(YBR015C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSS1(YMR023C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MTC4(YBR255W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:PCI8(YIL071C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PTC1(YDL006W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTC7(YHR076W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RCR1(YBR005W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REH1(YLR387C)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:REX4(YOL080C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RRI1(YDL216C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SEC72(YLR292C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLG1(YOR008C)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPS2(YDR522C)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRP40(YKR092C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSF2(YDR312W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SUM1(YDR310C)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TIP1(YBR067C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRM44(YPL030W)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VMA16(YHR026W)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YAL045C(YAL045C_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Gene:YCR102C(YCR102C_p)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YER184C(YER184C_p)|FD-Score:6.76|P-value:6.81E-12||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YGR228W(YGR228W_d)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR097C(YHR097C_p)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL102C(YIL102C_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function Gene:YJR020W(YJR020W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL071W(YKL071W_p)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR075C(YKR075C_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLL059C(YLL059C_d)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR131C(YOR131C_p)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR309C(YOR309C_d)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPK1(YKL126W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-5.16|P-value:1.21E-7||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:AQR1(YNL065W)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:CCP1(YKR066C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:DAP2(YHR028C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:DCN1(YLR128W)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DIG1(YPL049C)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DST1(YGL043W)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM1(YAL059W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm Gene:FLC1(YPL221W)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP10(YER182W_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HAL5(YJL165C)|FD-Score:4.76|P-value:9.77E-7||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:HLR1(YDR528W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; <br>HLR1 has a paralog, LRE1, that arose from the whole genome duplication Gene:JJJ1(YNL227C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:MNN2(YBR015C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSS1(YMR023C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:MTC4(YBR255W)|FD-Score:4.39|P-value:5.78E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:PCI8(YIL071C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PTC1(YDL006W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTC7(YHR076W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RCR1(YBR005W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REH1(YLR387C)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:REX4(YOL080C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Putative RNA exonuclease possibly involved in pre-rRNA processing and ribosome assembly Gene:RRI1(YDL216C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SEC72(YLR292C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLG1(YOR008C)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPS2(YDR522C)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SRP40(YKR092C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SSF2(YDR312W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:SUM1(YDR310C)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:TIP1(YBR067C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRM44(YPL030W)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VMA16(YHR026W)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YAL045C(YAL045C_d)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A Gene:YCR102C(YCR102C_p)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YER184C(YER184C_p)|FD-Score:6.76|P-value:6.81E-12||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YGR228W(YGR228W_d)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR097C(YHR097C_p)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL102C(YIL102C_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function Gene:YJR020W(YJR020W_d)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL071W(YKL071W_p)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKR075C(YKR075C_p)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLL059C(YLL059C_d)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR131C(YOR131C_p)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR309C(YOR309C_d)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPK1(YKL126W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-5.16|P-value:1.21E-7||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W9.261.02E-203.14LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W6.124.81E-101.53NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YJR012C_p4.582.28E-60.03YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YML069W4.562.61E-60.39POB3Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
YPR168W4.171.54E-50.76NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
YMR197C3.403.33E-40.06VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YLL011W3.344.12E-40.00SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YPL175W3.344.19E-40.10SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YHR188C3.245.90E-40.02GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YNL150W_d3.226.41E-40.16YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YGR114C_d3.060.001110.17YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YBL074C2.880.001960.05AAR2Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
YOR335C2.830.002328.12E-4ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
YNR054C2.830.002330.02ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YNL006W2.810.002500.06LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER184C_p6.766.81E-12YER184C_pPutative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source
YJL165C4.769.77E-7HAL5Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
YBR255W4.395.78E-6MTC4Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
YLR387C4.347.10E-6REH1Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
YOR131C_p4.337.48E-6YOR131C_pPutative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
YOR072W_d4.221.24E-5YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YBR067C4.211.29E-5TIP1Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
YGR228W_d4.022.86E-5YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
YPL030W4.013.10E-5TRM44tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
YNL227C3.944.09E-5JJJ1Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
YHR026W3.895.08E-5VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YNL065W3.759.01E-5AQR1Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YHR097C_p3.749.18E-5YHR097C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus
YMR023C3.701.07E-4MSS1Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
YIL071C3.681.18E-4PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain

GO enrichment analysis for SGTC_645
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2451.04E-81SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.2460S ribosome export
0.2311.87E-72SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.089743660S ribosome export
0.2227.95E-67SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.11702160S ribosome export
0.2208.25E-66SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.14285760S ribosome export
0.2086.29E-59SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.12676160S ribosome export
0.1905.31E-49SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.17948760S ribosome export
0.1887.85E-48SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.072289260S ribosome export
0.1714.79E-40SGTC_490717-0965 32.1 μMChemDiv (Drug-like library)58893490.098765460S ribosome export
0.1692.87E-39SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.11494360S ribosome export
0.1611.45E-35SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.077922160S ribosome export
0.1599.18E-35SGTC_31709103054 49.5 μMChembridge (Drug-like library)164329700.15384660S ribosome export
0.1583.63E-34SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.13333360S ribosome export
0.1574.99E-34SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.083333360S ribosome export
0.1552.92E-33SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.12643760S ribosome export
0.1532.22E-32SGTC_5700987-0065 62.6 μMChemDiv (Drug-like library)31044280.076923160S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_558k064-002721.3 μM0.4310345088526ChemDiv (Drug-like library)334.79895.55113DNA damage response
SGTC_6074491-098666.8 μM0.4698981ChemDiv (Drug-like library)296.793985.48512heme requiring
SGTC_1615st00265686.5 μM0.3333331584458TimTec (Natural product derivative library)231.253821.1424redox potentiating
SGTC_2863902873758.44 μM0.3333336460917Chembridge (Drug-like library)322.357823.0514
SGTC_3580075-00589.45 μM0.3235291069125ChemDiv (Drug-like library)378.255783.84822
SGTC_13833049-003220.7 μM0.31746712864ChemDiv (Drug-like library)291.347084.0261460S ribosome export
SGTC_344k064-00356.43 μM0.3050855088750ChemDiv (Drug-like library)339.217986.2321260S ribosome export
SGTC_7734358-145744.6 μM0.2957751186886ChemDiv (Drug-like library)352.38383.73716
SGTC_2866903424558.44 μM0.2898556462273Chembridge (Drug-like library)331.195984.41212
SGTC_5794491-1009110 μM0.2857141271041ChemDiv (Drug-like library)284.354444.7571260S ribosome export