0140-0236

N'-[[10-[[2-(dimethylamino)ethyl-methylamino]methyl]anthracen-9-yl]methyl]-N,N,N'-trimethylethane-1,2-diamine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_647
Screen concentration 81.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 990837
SMILES CN(C)CCN(C)CC1=C2C=CC=CC2=C(C3=CC=CC=C31)CN(C)CCN(C)C
Standardized SMILES CN(C)CCN(C)Cc1c2ccccc2c(CN(C)CCN(C)C)c3ccccc13
Molecular weight 406.6067
ALogP 4.07
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 24.69
% growth inhibition (Hom. pool) 13.73


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 990837
Download HIP data (tab-delimited text)  (excel)
Gene:IMP3(YHR148W)|FD-Score:5.05|P-value:2.23E-7|Clearance:0.43||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NHP2(YDL208W)|FD-Score:4.33|P-value:7.44E-6|Clearance:0.75||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:PRE7(YBL041W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RPP1(YHR062C)|FD-Score:5.08|P-value:1.91E-7|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCL1(YGL011C)|FD-Score:4.61|P-value:1.98E-6|Clearance:0.28||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC31(YDL195W)|FD-Score:8.91|P-value:2.54E-19|Clearance:3.83||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YOR203W(YOR203W_d)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPT1(YFL038C)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.1||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:IMP3(YHR148W)|FD-Score:5.05|P-value:2.23E-7|Clearance:0.43||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:NHP2(YDL208W)|FD-Score:4.33|P-value:7.44E-6|Clearance:0.75||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:PRE7(YBL041W)|FD-Score:3.58|P-value:1.72E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RPP1(YHR062C)|FD-Score:5.08|P-value:1.91E-7|Clearance:0.03||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCL1(YGL011C)|FD-Score:4.61|P-value:1.98E-6|Clearance:0.28||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC31(YDL195W)|FD-Score:8.91|P-value:2.54E-19|Clearance:3.83||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YOR203W(YOR203W_d)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.43||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPT1(YFL038C)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.1||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 990837
Download HOP data (tab-delimited text)  (excel)
Gene:BTT1(YDR252W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:CCE1(YKL011C)|FD-Score:4.85|P-value:6.21E-7||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CLB3(YDL155W)|FD-Score:3.09|P-value:0.00100||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COX8(YLR395C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DAN1(YJR150C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:EMC4(YGL231C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERP6(YGL002W)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:GAL80(YML051W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:HEF3(YNL014W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:IES1(YFL013C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC11(YOR013W_d)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:KSS1(YGR040W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:MDM38(YOL027C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MSN1(YOL116W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NAM8(YHR086W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NDI1(YML120C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:PAN2(YGL094C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PGU1(YJR153W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins Gene:PSP1(YDR505C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS19A(YOL121C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:THI3(YDL080C)|FD-Score:5.22|P-value:8.80E-8||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TMS1(YDR105C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TRM3(YDL112W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:UGA4(YDL210W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:VOA1(YGR106C)|FD-Score:5.73|P-value:4.98E-9||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:YBR072C-A(YBR072C-A_p)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR222W(YDR222W_p)|FD-Score:6.06|P-value:6.62E-10||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR387C(YDR387C_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative transporter, member of the sugar porter family; YDR387C is not an essential gene Gene:YFL042C(YFL042C_p)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL176C(YGL176C_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR168C(YGR168C_p)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHL037C(YHL037C_d)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR130C(YHR130C_d)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR177W(YHR177W_p)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YLR012C(YLR012C_p)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMR082C(YMR082C_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL057W(YOL057W)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL099C(YOL099C_d)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR127W(YPR127W)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPS7(YDR349C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:BTT1(YDR252W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:CCE1(YKL011C)|FD-Score:4.85|P-value:6.21E-7||SGD DESC:Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA Gene:CLB3(YDL155W)|FD-Score:3.09|P-value:0.00100||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COX8(YLR395C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DAN1(YJR150C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:EMC4(YGL231C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERP6(YGL002W)|FD-Score:4.54|P-value:2.78E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:GAL80(YML051W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Transcriptional regulator involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding Gene:HEF3(YNL014W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:IES1(YFL013C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRC11(YOR013W_d)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:KSS1(YGR040W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:MDM38(YOL027C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MSN1(YOL116W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NAM8(YHR086W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NDI1(YML120C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in the respiratory chain but does not pump protons, in contrast to the higher eukaryotic multisubunit respiratory complex I; phosphorylated; involved in Mn and H2O2 induced apoptosis; upon apoptotic stress, Ndip is activated in the mitochondria by N-terminal cleavage, and the truncated protein translocates to the cytoplasm to induce apoptosis; homolog of human AMID Gene:PAN2(YGL094C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PGU1(YJR153W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Endo-polygalacturonase, pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins Gene:PSP1(YDR505C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPO41(YFL036W)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation Gene:RPS19A(YOL121C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:THI3(YDL080C)|FD-Score:5.22|P-value:8.80E-8||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TMS1(YDR105C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TRM3(YDL112W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:UGA4(YDL210W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:VOA1(YGR106C)|FD-Score:5.73|P-value:4.98E-9||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:YBR072C-A(YBR072C-A_p)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR222W(YDR222W_p)|FD-Score:6.06|P-value:6.62E-10||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR387C(YDR387C_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative transporter, member of the sugar porter family; YDR387C is not an essential gene Gene:YFL042C(YFL042C_p)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YGL176C(YGL176C_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR168C(YGR168C_p)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHL037C(YHL037C_d)|FD-Score:-3.48|P-value:2.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR130C(YHR130C_d)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR177W(YHR177W_p)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YLR012C(YLR012C_p)|FD-Score:-3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMR082C(YMR082C_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL057W(YOL057W)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL099C(YOL099C_d)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YPR127W(YPR127W)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPS7(YDR349C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W8.912.54E-193.83SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YHR062C5.081.91E-70.03RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR148W5.052.23E-70.43IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YGL011C4.611.98E-60.28SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YDL208W4.337.44E-60.75NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YBL041W3.581.72E-40.00PRE7Beta 6 subunit of the 20S proteasome
YOR203W_d3.581.74E-40.43YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
YFL038C3.148.31E-40.10YPT1Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
YKL182W3.040.001180.03FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YLR230W_d3.010.001320.04YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YDL055C2.970.001490.01PSA1GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
YFR031C2.960.001550.10SMC2Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
YDL029W2.850.002160.15ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YJL001W2.700.003430.04PRE3Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
YPR104C2.660.003880.03FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR222W_p6.066.62E-10YDR222W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YGR106C5.734.98E-9VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YDL080C5.228.80E-8THI3Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis
YFL042C_p5.012.76E-7YFL042C_pPutative protein of unknown function; YFL042C is not an essential gene
YKL011C4.856.21E-7CCE1Mitochondrial cruciform cutting endonuclease, cleaves Holliday junctions formed during recombination of mitochondrial DNA
YFL036W4.741.07E-6RPO41Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
YGL002W4.542.78E-6ERP6Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
YDR505C3.993.28E-5PSP1Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
YPR127W3.807.30E-5YPR127WPutative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YHR031C3.797.49E-5RRM3DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p
YLR395C3.787.95E-5COX8Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YOL099C_d3.729.79E-5YOL099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes
YDR349C3.721.00E-4YPS7Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
YDR105C3.691.13E-4TMS1Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
YDR252W3.671.23E-4BTT1Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3

GO enrichment analysis for SGTC_647
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2426.08E-79SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.0588235
0.2111.91E-60SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.111111
0.1822.50E-45SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.0862069
0.1723.21E-40SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0545455
0.1404.92E-27SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0862069
0.1373.38E-26SGTC_1260417-1624 672.5 μMChemDiv (Drug-like library)419120.0754717DNA intercalators
0.1271.73E-22SGTC_525k781-2675 26.7 μMChemDiv (Drug-like library)36511180.0961538redox potentiating
0.1231.75E-21SGTC_31979113331 49.5 μMChembridge (Drug-like library)166104590.102941
0.1133.52E-18SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.0961538
0.1111.54E-17SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.0793651RPP1 & pyrimidine depletion
0.1102.96E-17SGTC_12670828-0272 9.5 μMChemDiv (Drug-like library)7453340.0754717
0.1089.54E-17SGTC_28057950783 58.4 μMChembridge (Drug-like library)11789580.0757576
0.1062.93E-16SGTC_12680828-0275 2.5 μMChemDiv (Drug-like library)7453370.0961538
0.1031.52E-15SGTC_389tpck 16.7 μMMiscellaneous4396470.127273
0.0953.13E-13SGTC_3111315-0136 58.0 μMChemDiv (Drug-like library)67592070.0793651

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1681tripelennamine100 μM0.359066NIH Clinical Collection291.818983.48413
SGTC_1680diphenhydramine100 μM0.3333338980NIH Clinical Collection291.815683.76612NEO1
SGTC_2730imipramine71.33 μM0.33696Miscellaneous280.407264.38702
SGTC_2736butenafine14.65 μM0.2954552484Miscellaneous317.467185.79501
SGTC_15977-benzyloxygramine47.5 μM0.270833260807TimTec (Pure natural product library)280.36423.76412calcium & mitochondrial duress
SGTC_1089terbinafine2.25 μM0.2653065282481NIH Clinical Collection327.890845.71711
SGTC_2776terbinafine19.92 μM0.2653065282481NIH Clinical Collection327.890845.71711cell wall signaling
SGTC_12420402-010360.1 μM0.263158432868ChemDiv (Drug-like library)191.269482.3102
SGTC_270tamoxifen5.46 μM0.260875376Miscellaneous371.514566.31902NEO1
SGTC_2774tamoxifen10.92 μM0.260872733526Miscellaneous371.514566.31902
SGTC_870100-005235.5 μM0.25641200187ChemDiv (Drug-like library)256.138962.57202
SGTC_15091326-113180 μM0.25641768313ChemDiv (Drug-like library)239.312283.09402