1328-0195

4,5-dibromo-4,5-dihydrobenzo[1,2]cyclohepta[3,4-b]thiophen-10-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_650
Screen concentration 38.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2780821
SMILES C1=CC=C2C(=C1)C(C(C3=C(C2=O)C=CS3)Br)Br
Standardized SMILES BrC1C(Br)c2sccc2C(=O)c3ccccc13
Molecular weight 372.075
ALogP 4.45
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.07
% growth inhibition (Hom. pool) 7.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2780821
Download HIP data (tab-delimited text)  (excel)
Gene:CBF5(YLR175W)|FD-Score:3.86|P-value:5.63E-5|Clearance:0.02||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CCT4(YDL143W)|FD-Score:5.44|P-value:2.74E-8|Clearance:1.57||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC23(YHR166C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:HIP1(YGR191W)|FD-Score:-3.2|P-value:6.81E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:IPL1(YPL209C)|FD-Score:3.85|P-value:6.00E-5|Clearance:0.34||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:LTO1(YNL260C_p)|FD-Score:3.2|P-value:6.81E-4|Clearance:0||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MPS3(YJL019W)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.23||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NOP56(YLR197W)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.12||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRE1(YER012W)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.07||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RPP1(YHR062C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:CBF5(YLR175W)|FD-Score:3.86|P-value:5.63E-5|Clearance:0.02||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CCT4(YDL143W)|FD-Score:5.44|P-value:2.74E-8|Clearance:1.57||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC23(YHR166C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:HIP1(YGR191W)|FD-Score:-3.2|P-value:6.81E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:IPL1(YPL209C)|FD-Score:3.85|P-value:6.00E-5|Clearance:0.34||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:LTO1(YNL260C_p)|FD-Score:3.2|P-value:6.81E-4|Clearance:0||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MPS3(YJL019W)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.23||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NOP56(YLR197W)|FD-Score:3.2|P-value:6.83E-4|Clearance:0.12||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRE1(YER012W)|FD-Score:3.5|P-value:2.29E-4|Clearance:0.07||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RPP1(YHR062C)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2780821
Download HOP data (tab-delimited text)  (excel)
Gene:ADE16(YLR028C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AFR1(YDR085C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:ATG29(YPL166W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATX2(YOR079C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BCH1(YMR237W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF130(YGR134W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CBP2(YHL038C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:DAL81(YIR023W)|FD-Score:5.73|P-value:4.94E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DON1(YDR273W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:GCN5(YGR252W)|FD-Score:-3.76|P-value:8.42E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HSV2(YGR223C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:ILM1(YJR118C)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INO4(YOL108C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:KEL3(YPL263C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LEO1(YOR123C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LSM1(YJL124C)|FD-Score:-3.77|P-value:8.12E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MID2(YLR332W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL23(YOR150W)|FD-Score:7.84|P-value:2.19E-15||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO4(YAL029C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:OPI9(YLR338W_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAC2(YER007W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET112(YBL080C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET494(YNR045W)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX29(YDR479C)|FD-Score:9.71|P-value:1.38E-22||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFK26(YIL107C)|FD-Score:8.36|P-value:3.13E-17||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHO85(YPL031C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLB3(YOL011W)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PPZ1(YML016C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRM4(YPL156C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PRX1(YBL064C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:RAS2(YNL098C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RBG2(YGR173W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RCR2(YDR003W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SCW10(YMR305C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SCW4(YGR279C)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SFL1(YOR140W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SGT2(YOR007C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SPC1(YJR010C-A)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPS19(YNL202W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:STB6(YKL072W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:STL1(YDR536W)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:SWE1(YJL187C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TAT1(YBR069C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRM44(YPL030W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VAC7(YNL054W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VBA5(YKR105C_p)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VOA1(YGR106C)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS25(YJR102C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YDR056C(YDR056C_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR109C(YDR109C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative kinase Gene:YDR124W(YDR124W_p)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR220C(YDR220C_d)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGR017W(YGR017W_p)|FD-Score:4.83|P-value:6.99E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YHR112C(YHR112C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL067C(YIL067C_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJR098C(YJR098C_p)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL031W(YKL031W_d)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL131W(YKL131W_d)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR104W(YKR104W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR042C(YLR042C_p)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR063W(YLR063W_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YML090W(YML090W_d)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR230W-A(YMR230W-A_p)|FD-Score:5.08|P-value:1.91E-7||SGD DESC:Putative protein of unknown function Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YPL257W(YPL257W_p)|FD-Score:-3.15|P-value:8.27E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR126C(YPR126C_d)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE16(YLR028C)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AFR1(YDR085C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication Gene:ATG29(YPL166W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATX2(YOR079C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation Gene:BCH1(YMR237W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF130(YGR134W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CBP2(YHL038C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene Gene:DAL81(YIR023W)|FD-Score:5.73|P-value:4.94E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DON1(YDR273W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:GCN5(YGR252W)|FD-Score:-3.76|P-value:8.42E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HSV2(YGR223C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:ILM1(YJR118C)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INO4(YOL108C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:KEL3(YPL263C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LEO1(YOR123C)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LSM1(YJL124C)|FD-Score:-3.77|P-value:8.12E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MID2(YLR332W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MRPL23(YOR150W)|FD-Score:7.84|P-value:2.19E-15||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MYO4(YAL029C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:OPI9(YLR338W_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAC2(YER007W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PET112(YBL080C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET494(YNR045W)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX29(YDR479C)|FD-Score:9.71|P-value:1.38E-22||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PFK26(YIL107C)|FD-Score:8.36|P-value:3.13E-17||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHO85(YPL031C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PLB3(YOL011W)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PPZ1(YML016C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRM4(YPL156C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PRX1(YBL064C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:RAS2(YNL098C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RBG2(YGR173W)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RCR2(YDR003W)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SCW10(YMR305C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SCW4(YGR279C)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating Gene:SFL1(YOR140W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SGT2(YOR007C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SPC1(YJR010C-A)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SPS19(YNL202W)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:STB6(YKL072W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:STL1(YDR536W)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:SWE1(YJL187C)|FD-Score:4.29|P-value:9.00E-6||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TAT1(YBR069C)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRM44(YPL030W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:VAC7(YNL054W)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VBA5(YKR105C_p)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VOA1(YGR106C)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS25(YJR102C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YDR056C(YDR056C_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR109C(YDR109C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative kinase Gene:YDR124W(YDR124W_p)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Putative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor Gene:YDR220C(YDR220C_d)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGR017W(YGR017W_p)|FD-Score:4.83|P-value:6.99E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YHR112C(YHR112C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL067C(YIL067C_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YJR098C(YJR098C_p)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKL031W(YKL031W_d)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL131W(YKL131W_d)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR104W(YKR104W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR042C(YLR042C_p)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR063W(YLR063W_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YML090W(YML090W_d)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR230W-A(YMR230W-A_p)|FD-Score:5.08|P-value:1.91E-7||SGD DESC:Putative protein of unknown function Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YPL257W(YPL257W_p)|FD-Score:-3.15|P-value:8.27E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPR126C(YPR126C_d)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL143W5.442.74E-81.57CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR175W3.865.63E-50.02CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YPL209C3.856.00E-50.34IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YHR166C3.512.27E-40.00CDC23Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YER012W3.502.29E-40.07PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YJL019W3.433.00E-40.23MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YNL260C_p3.206.81E-48.56E-4LTO1_pEssential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YLR197W3.206.83E-40.12NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YIL019W3.080.001030.09FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YER094C2.990.001380.06PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YOL038W2.930.001670.03PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YMR235C2.900.001843.61E-4RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YFR029W2.900.001840.03PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YOR159C2.870.002030.02SME1Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
YBR198C2.850.002180.05TAF5Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR479C9.711.38E-22PEX29Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
YIL107C8.363.13E-17PFK266-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A
YOR150W7.842.19E-15MRPL23Mitochondrial ribosomal protein of the large subunit
YIR023W5.734.94E-9DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YMR230W-A_p5.081.91E-7YMR230W-A_pPutative protein of unknown function
YGR106C5.042.34E-7VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YGR017W_p4.836.99E-7YGR017W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
YDR536W4.572.38E-6STL1Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
YDR124W_p4.533.01E-6YDR124W_pPutative protein of unknown function; non-essential gene; expression is strongly induced by alpha factor
YLR042C_p4.464.03E-6YLR042C_pProtein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene
YOR123C4.464.04E-6LEO1Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YNR045W4.415.20E-6PET494Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
YJL187C4.299.00E-6SWE1Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
YPR126C_d4.161.56E-5YPR126C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBL080C4.161.58E-5PET112Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog

GO enrichment analysis for SGTC_650
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1553.80E-33SGTC_7431275-0778 158.0 μMChemDiv (Drug-like library)28492570.107143excess fatty acid
0.1461.70E-29SGTC_12550566-0045 221.0 μMChemDiv (Drug-like library)27884690.0833333excess fatty acid
0.1271.28E-22SGTC_1309pyrazolanthrone 13.4 μMChemDiv (Drug-like library)85150.234043
0.1202.34E-20SGTC_486niguldipine 82.0 μMMiscellaneous12360.0786517amide catabolism
0.1133.96E-18SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.0793651amide catabolism
0.1023.27E-15SGTC_24605552655 174.2 μMMiscellaneous54019890.1amide catabolism
0.1017.08E-15SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.0952381amide catabolism
0.0891.12E-11SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0655738
0.0847.95E-11SGTC_33309141424 71.4 μMChembridge (Drug-like library)272610200.0857143amide catabolism
0.0831.69E-10SGTC_3252810-4341 181.0 μMChemDiv (Drug-like library)7749700.0461538amide catabolism
0.0831.75E-10SGTC_3753970-0961 9.3 μMChemDiv (Drug-like library)28982220.0897436tubulin folding & SWR complex
0.0831.81E-10SGTC_6530203-0016 55.2 μMChemDiv (Drug-like library)2953020.101695
0.0823.02E-10SGTC_10563448-1962 115.0 μMChemDiv (Drug-like library)7155070.0847458amide catabolism
0.0815.43E-10SGTC_1710st031758 61.5 μMTimTec (Natural product derivative library)53943410.0694444
0.0772.62E-9SGTC_11871391-0724 117.0 μMChemDiv (Drug-like library)69979860.0821918amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1693xanthone101.9 μM0.2972977020TimTec (Natural product derivative library)196.201422.96402DNA damage response
SGTC_2532xanthone78.9 μM0.2972977020Microsource (Natural product library)196.201422.96402
SGTC_1915st06710530.6 μM0.28205169751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_263alizarin107.93 μM0.2558146293Miscellaneous240.210922.32424iron homeostasis
SGTC_2686alizarin95.37 μM0.2558146293Miscellaneous240.210922.32424RPP1 & pyrimidine depletion
SGTC_3710195-00067.36 μM0.253928593ChemDiv (Drug-like library)253.209682.70204
SGTC_2671danthron100 μM0.252950Microsource (Natural product library)240.210922.32424DNA damage response
SGTC_9143820-6779408 μM0.2456142893481ChemDiv (Drug-like library)392.450884.69725
SGTC_21085365811200 μM0.244444780283Chembridge (Fragment library)213.231941.21213
SGTC_2681menadione3.18 μM0.2439024055Miscellaneous172.180022.20402superoxide
SGTC_394menadione8.33 μM0.2439024055Miscellaneous172.180022.20402superoxide
SGTC_1889st0590805.18 μM0.24176925TimTec (Natural product derivative library)240.253982.85713
SGTC_1309pyrazolanthrone13.4 μM0.2340438515ChemDiv (Drug-like library)220.226122.99412