1124-0271

5-bromo-N-[(Z)-furan-2-ylmethylideneamino]-2-hydroxybenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_654
Screen concentration 80.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 5403748
SMILES C1=COC(=C1)C=NNC(=O)C2=C(C=CC(=C2)Br)O
Standardized SMILES Oc1ccc(Br)cc1C(=O)NN=Cc2occc2
Molecular weight 309.1155
ALogP 2.59
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 30.17
% growth inhibition (Hom. pool) 6.45


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5403748
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:3.92|P-value:4.49E-5|Clearance:0.08||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:HEM1(YDR232W)|FD-Score:9.2|P-value:1.87E-20|Clearance:3.24||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:NIP1(YMR309C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.03||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP14(YDL148C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.04||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUP192(YJL039C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.08||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:ORC3(YLL004W)|FD-Score:5.96|P-value:1.28E-9|Clearance:2.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:POP5(YAL033W)|FD-Score:3.25|P-value:5.74E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PSF3(YOL146W)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.01||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RLP7(YNL002C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPC11(YDR045C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL18A(YOL120C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS13(YDR064W)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.13||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS20(YHL015W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:3.75|P-value:8.74E-5|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SMD2(YLR275W)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.01||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TIF6(YPR016C)|FD-Score:3.14|P-value:8.49E-4|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:UTP13(YLR222C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.06||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPL142C(YPL142C_d)|FD-Score:3.2|P-value:6.95E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:CCT4(YDL143W)|FD-Score:3.92|P-value:4.49E-5|Clearance:0.08||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:HEM1(YDR232W)|FD-Score:9.2|P-value:1.87E-20|Clearance:3.24||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:NIP1(YMR309C)|FD-Score:3.17|P-value:7.62E-4|Clearance:0.03||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP14(YDL148C)|FD-Score:3.38|P-value:3.66E-4|Clearance:0.04||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUP192(YJL039C)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.08||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:ORC3(YLL004W)|FD-Score:5.96|P-value:1.28E-9|Clearance:2.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:POP5(YAL033W)|FD-Score:3.25|P-value:5.74E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PSF3(YOL146W)|FD-Score:3.18|P-value:7.25E-4|Clearance:0.01||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RLP7(YNL002C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPC11(YDR045C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL18A(YOL120C)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS13(YDR064W)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.13||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS20(YHL015W)|FD-Score:3.84|P-value:6.24E-5|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:3.75|P-value:8.74E-5|Clearance:0.1||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:SMD2(YLR275W)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.01||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TIF6(YPR016C)|FD-Score:3.14|P-value:8.49E-4|Clearance:0.07||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:UTP13(YLR222C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.06||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YPL142C(YPL142C_d)|FD-Score:3.2|P-value:6.95E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5403748
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AIM22(YJL046W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:4.85|P-value:6.29E-7||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:APL4(YPR029C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:AVT3(YKL146W)|FD-Score:4.79|P-value:8.26E-7||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BTS1(YPL069C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDA2(YLR308W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CKA1(YIL035C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLB3(YDL155W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CRT10(YOL063C)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CWP2(YKL096W-A)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:CYC7(YEL039C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL7(YIR031C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DPP1(YDR284C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:EAF1(YDR359C)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EMC5(YIL027C)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FAT3(YKL187C_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FDC1(YDR539W)|FD-Score:4.76|P-value:9.66E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FMP40(YPL222W_p)|FD-Score:4.27|P-value:9.87E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPA2(YER020W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPX1(YKL026C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:HDA3(YPR179C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HSV2(YGR223C)|FD-Score:5.73|P-value:5.12E-9||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HTD2(YHR067W)|FD-Score:4.29|P-value:8.76E-6||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT17(YNR072W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:ISC1(YER019W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:IWR1(YDL115C)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LIP2(YLR239C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MEI4(YER044C-A)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MET17(YLR303W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MMP1(YLL061W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MPP6(YNR024W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL11(YDL202W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSL1(YIR009W)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NEW1(YPL226W)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NTH2(YBR001C)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PEP7(YDR323C)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PER1(YCR044C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PHO88(YBR106W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RAD23(YEL037C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:REV3(YPL167C)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHR2(YIL053W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL14A(YKL006W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SDS23(YGL056C)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SER3(YER081W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:7.51|P-value:2.85E-14||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIF4631(YGR162W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TRM732(YMR259C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TUM1(YOR251C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VAC17(YCL063W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS25(YJR102C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:4.33|P-value:7.55E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS29(YHR012W)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS4(YPR173C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YAK1(YJL141C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAR035C-A(YAR035C-A_p)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR056W-A(YBR056W-A_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YEN1(YER041W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YGR126W(YGR126W_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR242W(YGR242W_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR078W(YHR078W_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YHR112C(YHR112C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL012W(YIL012W_d)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR078W(YKR078W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR415C(YLR415C_p)|FD-Score:6.45|P-value:5.68E-11||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML090W(YML090W_d)|FD-Score:-4.39|P-value:5.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YNL303W(YNL303W_d)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR053W(YOR053W_d)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPL071C(YPL071C_p)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL182C(YPL182C_d)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPL225W(YPL225W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:ADH6(YMR318C)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:AIM22(YJL046W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALD6(YPL061W)|FD-Score:4.85|P-value:6.29E-7||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:APL4(YPR029C)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:AVT3(YKL146W)|FD-Score:4.79|P-value:8.26E-7||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BTS1(YPL069C)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDA2(YLR308W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CKA1(YIL035C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLB3(YDL155W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CRT10(YOL063C)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:CWP2(YKL096W-A)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:CYC7(YEL039C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAL7(YIR031C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DPP1(YDR284C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:EAF1(YDR359C)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EMC5(YIL027C)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:FAT3(YKL187C_p)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies Gene:FDC1(YDR539W)|FD-Score:4.76|P-value:9.66E-7||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FMP40(YPL222W_p)|FD-Score:4.27|P-value:9.87E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPA2(YER020W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GPX1(YKL026C)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:HDA3(YPR179C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HSV2(YGR223C)|FD-Score:5.73|P-value:5.12E-9||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HTD2(YHR067W)|FD-Score:4.29|P-value:8.76E-6||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT17(YNR072W)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Hexose transporter, up-regulated in media containing raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose Gene:ISC1(YER019W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Mitochondrial membrane localized inositol phosphosphingolipid phospholipase C, hydrolyzes complex sphingolipids to produce ceramide; activated by phosphatidylserine, cardiolipin, and phosphatidylglycerol; mediates Na+ and Li+ halotolerance Gene:IWR1(YDL115C)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LIP2(YLR239C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MEI4(YER044C-A)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MET17(YLR303W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MMP1(YLL061W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:High-affinity S-methylmethionine permease, required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p Gene:MPP6(YNR024W)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL11(YDL202W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSL1(YIR009W)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members Gene:NEW1(YPL226W)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NTH2(YBR001C)|FD-Score:4.29|P-value:8.85E-6||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PEP7(YDR323C)|FD-Score:3.73|P-value:9.68E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PER1(YCR044C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PHO88(YBR106W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:RAD23(YEL037C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:REV3(YPL167C)|FD-Score:3.78|P-value:8.00E-5||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHR2(YIL053W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL14A(YKL006W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:-3.8|P-value:7.22E-5||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SDS23(YGL056C)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:One of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; involved in cell separation during budding; SDS23 has a paralog, SDS24, that arose from the whole genome duplication Gene:SER3(YER081W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER3 has a paralog, SER33, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:7.51|P-value:2.85E-14||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIF4631(YGR162W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TRM732(YMR259C_p)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TUM1(YOR251C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:VAC17(YCL063W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS25(YJR102C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:4.33|P-value:7.55E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS29(YHR012W)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS4(YPR173C)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:YAK1(YJL141C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YAR035C-A(YAR035C-A_p)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBR056W-A(YBR056W-A_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YEN1(YER041W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YGR126W(YGR126W_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YGR242W(YGR242W_d)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR078W(YHR078W_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YHR112C(YHR112C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YIL012W(YIL012W_d)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR078W(YKR078W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR415C(YLR415C_p)|FD-Score:6.45|P-value:5.68E-11||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YML002W(YML002W_p)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML090W(YML090W_d)|FD-Score:-4.39|P-value:5.68E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YNL303W(YNL303W_d)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR053W(YOR053W_d)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPL071C(YPL071C_p)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL182C(YPL182C_d)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPL225W(YPL225W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR232W9.201.87E-203.24HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YLL004W5.961.28E-92.04ORC3Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YDL143W3.924.49E-50.08CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YHL015W3.846.24E-50.08RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YNL178W3.758.74E-50.10RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YLR275W3.651.30E-40.01SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YDR064W3.641.35E-40.13RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YJL039C3.512.22E-40.08NUP192Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
YLR222C3.442.95E-40.06UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDL148C3.383.66E-40.04NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YOL120C3.344.15E-40.08RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YDR045C3.265.58E-40.01RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YAL033W3.255.74E-40.05POP5Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL142C_d3.206.95E-40.01YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YNL002C3.197.16E-40.00RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR069C7.512.85E-14TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YLR415C_p6.455.68E-11YLR415C_pPutative protein of unknown function; YLR415C is not an essential gene
YGR223C5.735.12E-9HSV2Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization
YAR035C-A_p5.571.28E-8YAR035C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis
YDL115C5.171.15E-7IWR1RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
YHR012W5.151.28E-7VPS29Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
YPL061W4.856.29E-7ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YKL146W4.798.26E-7AVT3Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YDR539W4.769.66E-7FDC1Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm
YIR031C4.741.08E-6DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YPL069C4.691.39E-6BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YPL182C_d4.661.56E-6YPL182C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
YNL303W_d4.464.16E-6YNL303W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YIL012W_d4.385.88E-6YIL012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL065W4.337.55E-6VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p

GO enrichment analysis for SGTC_654
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1271.25E-22SGTC_5840986-0246 15.4 μMChemDiv (Drug-like library)533150.0923077heme requiring
0.0863.82E-11SGTC_6074491-0986 66.8 μMChemDiv (Drug-like library)6989810.0833333heme requiring
0.0831.63E-10SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.0759494redox potentiating
0.0814.41E-10SGTC_171k064-0041 9.7 μMChemDiv (Drug-like library)533250.0793651heme requiring
0.0806.44E-10SGTC_1025k007-0731 70.0 μMChemDiv (Drug-like library)62561180.140625
0.0773.25E-9SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0857143
0.0741.24E-8SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.078125heme biosynthesis & mitochondrial translocase
0.0741.40E-8SGTC_1073triclabendazole 3.1 μMNIH Clinical Collection502480.0657895
0.0664.05E-7SGTC_30049076122 71.4 μMChembridge (Drug-like library)447825510.152778
0.0622.20E-6SGTC_30079078383 71.4 μMChembridge (Drug-like library)64969950.130952
0.0612.44E-6SGTC_13391486-1321 22.7 μMChemDiv (Drug-like library)34693820.103896ERG2
0.0603.29E-6SGTC_2730imipramine 71.3 μMMiscellaneous36960.0606061
0.0594.88E-6SGTC_1613st002381 52.1 μMTimTec (Natural product derivative library)5511670.097561
0.0587.29E-6SGTC_23799035213 200.0 μMChembridge (Fragment library)6465510.0694444
0.0588.13E-6SGTC_3783970-0959 8.3 μMChemDiv (Drug-like library)66156530.0823529

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12201838-0075193 μM0.5454555529645ChemDiv (Drug-like library)379.20533.15625
SGTC_7531322-0200289 μM0.4310345403907ChemDiv (Drug-like library)275.216961.85526
SGTC_3021094-006318.9 μM0.426235440289ChemDiv (Drug-like library)374.188782.47624
SGTC_2230bromochlorosalicylanilide954.5 nM0.42307777254Miscellaneous326.573063.78522
SGTC_8981000-084928.3 μM0.4210536751807ChemDiv (Drug-like library)356.374042.72334
SGTC_11780988-0033191 μM0.3833335392207ChemDiv (Drug-like library)349.179323.17324
SGTC_30833-058795.58 μM0.3584915382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_9781416-051287.4 μM0.356814003ChemDiv (Drug-like library)270.283260.69424redox potentiating
SGTC_12700831-117017.3 μM0.356764716ChemDiv (Drug-like library)380.234664.25113
SGTC_310508-197457.24 μM0.343756810525ChemDiv (Drug-like library)414.679724.6213