1304-0007

2-sulfanylidene-[1,3]dithiolo[4,5-b][1,4]dithiepin-6-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_661
Screen concentration 33.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 3117130
SMILES C1C(=O)CSC2=C(S1)SC(=S)S2
Standardized SMILES O=C1CSC2=C(SC1)SC(=S)S2
Molecular weight 252.4204
ALogP 3.62
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.36
% growth inhibition (Hom. pool) 9.37


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3117130
Download HIP data (tab-delimited text)  (excel)
Gene:BCP1(YDR361C)|FD-Score:3.9|P-value:4.84E-5|Clearance:0.17||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC27(YBL084C)|FD-Score:5.23|P-value:8.48E-8|Clearance:1.12||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DAD2(YKR083C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.22||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG8(YMR220W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.2||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ESP1(YGR098C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.05||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI11(YDR302W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.05||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:ILV5(YLR355C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.04||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:ORC4(YPR162C)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PDS5(YMR076C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.21||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP9(YDL030W)|FD-Score:3.73|P-value:9.45E-5|Clearance:0.07||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RIO2(YNL207W)|FD-Score:5.46|P-value:2.36E-8|Clearance:1.12||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPP1(YHR062C)|FD-Score:-4.24|P-value:1.12E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SGD1(YLR336C)|FD-Score:3.28|P-value:5.21E-4|Clearance:0.06||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SWI1(YPL016W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.01||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:BCP1(YDR361C)|FD-Score:3.9|P-value:4.84E-5|Clearance:0.17||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC27(YBL084C)|FD-Score:5.23|P-value:8.48E-8|Clearance:1.12||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DAD2(YKR083C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.22||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERG8(YMR220W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.2||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ESP1(YGR098C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.05||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI11(YDR302W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.05||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:ILV5(YLR355C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.04||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:ORC4(YPR162C)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PDS5(YMR076C)|FD-Score:3.22|P-value:6.39E-4|Clearance:0.21||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP9(YDL030W)|FD-Score:3.73|P-value:9.45E-5|Clearance:0.07||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RIO2(YNL207W)|FD-Score:5.46|P-value:2.36E-8|Clearance:1.12||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPP1(YHR062C)|FD-Score:-4.24|P-value:1.12E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SGD1(YLR336C)|FD-Score:3.28|P-value:5.21E-4|Clearance:0.06||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SWI1(YPL016W)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.01||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3117130
Download HOP data (tab-delimited text)  (excel)
Gene:ALR2(YFL050C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:APL1(YJR005W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BNI4(YNL233W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:CBP1(YJL209W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CBP4(YGR174C)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CBR1(YIL043C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CHS3(YBR023C)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CNA1(YLR433C)|FD-Score:7.27|P-value:1.80E-13||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COX7(YMR256C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTR9(YOL145C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DCG1(YIR030C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DGR1(YNL130C-A_p)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DYN1(YKR054C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM32(YER176W)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EMI1(YDR512C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG4(YGL012W)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY21(YER060W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FYV12(YOR183W_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV8(YGR196C)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN3(YKR026C)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HBN1(YCL026C-B_p)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:IDP1(YDL066W)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:ILV1(YER086W)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:MDM36(YPR083W)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MRF1(YGL143C)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:OPT2(YPR194C)|FD-Score:4.49|P-value:3.53E-6||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAR32(YDL173W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX3(YDR329C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PHO5(YBR093C)|FD-Score:5.53|P-value:1.65E-8||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO88(YBR106W)|FD-Score:7.81|P-value:2.78E-15||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PLB3(YOL011W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PRO2(YOR323C)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUF2(YPR042C)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAD4(YER162C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD51(YER095W)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RNP1(YLL046C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPS29A(YLR388W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SDS3(YIL084C)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SLX9(YGR081C)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNX4(YJL036W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOD1(YJR104C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPT7(YBR081C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB2(YMR053C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:STP22(YCL008C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TOF1(YNL273W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TOM5(YPR133W-A)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TRP4(YDR354W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP15(YMR304W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VPS25(YJR102C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YER038W-A(YER038W-A_d)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGL117W(YGL117W_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function Gene:YHR202W(YHR202W_p)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIH1(YCR059C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL058W(YIL058W_d)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR075C(YKR075C_p)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR415C(YLR415C_p)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YMR304C-A(YMR304C-A_d)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL089C(YNL089C_d)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YPR064W(YPR064W_d)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSP3(YOR003W)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ZAP1(YJL056C)|FD-Score:5.85|P-value:2.46E-9||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ALR2(YFL050C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition Gene:APL1(YJR005W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BNI4(YNL233W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:CBP1(YJL209W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CBP4(YGR174C)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CBR1(YIL043C)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CHS3(YBR023C)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CNA1(YLR433C)|FD-Score:7.27|P-value:1.80E-13||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COX7(YMR256C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTR9(YOL145C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DCG1(YIR030C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DGR1(YNL130C-A_p)|FD-Score:-3.83|P-value:6.37E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DYN1(YKR054C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:ECM32(YER176W)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:EMI1(YDR512C)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG4(YGL012W)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY21(YER060W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FYV12(YOR183W_p)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV8(YGR196C)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN3(YKR026C)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HBN1(YCL026C-B_p)|FD-Score:4.3|P-value:8.47E-6||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:IDP1(YDL066W)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:ILV1(YER086W)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:MDM36(YPR083W)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MRF1(YGL143C)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:OPT2(YPR194C)|FD-Score:4.49|P-value:3.53E-6||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAR32(YDL173W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX3(YDR329C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PHO5(YBR093C)|FD-Score:5.53|P-value:1.65E-8||SGD DESC:Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2 Gene:PHO88(YBR106W)|FD-Score:7.81|P-value:2.78E-15||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PLB3(YOL011W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PRO2(YOR323C)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUF2(YPR042C)|FD-Score:3.78|P-value:7.83E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:RAD4(YER162C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD51(YER095W)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RNP1(YLL046C)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPS29A(YLR388W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SDS3(YIL084C)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SLX9(YGR081C)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNX4(YJL036W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOD1(YJR104C)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPT7(YBR081C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SSD1(YDR293C)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STB2(YMR053C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:STP22(YCL008C)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:TOF1(YNL273W)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TOM5(YPR133W-A)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TRP4(YDR354W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBP15(YMR304W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:VPS25(YJR102C)|FD-Score:-3.12|P-value:8.99E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YER038W-A(YER038W-A_d)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YGL117W(YGL117W_p)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Putative protein of unknown function Gene:YHR202W(YHR202W_p)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIH1(YCR059C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL058W(YIL058W_d)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR075C(YKR075C_p)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR415C(YLR415C_p)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Putative protein of unknown function; YLR415C is not an essential gene Gene:YMR304C-A(YMR304C-A_d)|FD-Score:4.57|P-value:2.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL089C(YNL089C_d)|FD-Score:3.84|P-value:6.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YPR064W(YPR064W_d)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSP3(YOR003W)|FD-Score:-3.95|P-value:3.91E-5||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ZAP1(YJL056C)|FD-Score:5.85|P-value:2.46E-9||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL207W5.462.36E-81.12RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YBL084C5.238.48E-81.12CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YKR083C4.111.94E-50.22DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YDR361C3.904.84E-50.17BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDL030W3.739.45E-50.07PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YPL016W3.661.26E-40.01SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YGR098C3.651.29E-40.00ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YLR355C3.651.30E-40.04ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YPR162C3.611.51E-40.04ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YER133W3.571.75E-40.05GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDR302W3.532.09E-40.05GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YMR220W3.482.54E-40.20ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YLR336C3.285.21E-40.06SGD1Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
YMR076C3.226.39E-40.21PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YPR086W3.010.001300.05SUA7Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR106W7.812.78E-15PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YLR433C7.271.80E-13CNA1Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
YJL056C5.852.46E-9ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
YBR093C5.531.65E-8PHO5Repressible acid phosphatase (1 of 3) that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
YOR323C5.052.25E-7PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YEL009C4.953.64E-7GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YKR075C_p4.943.86E-7YKR075C_pProtein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YLR415C_p4.836.95E-7YLR415C_pPutative protein of unknown function; YLR415C is not an essential gene
YPR064W_d4.731.14E-6YPR064W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YNL273W4.711.25E-6TOF1Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase
YMR304C-A_d4.572.48E-6YMR304C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10
YPR194C4.493.53E-6OPT2Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles
YPR083W4.493.60E-6MDM36Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
YGL143C4.473.97E-6MRF1Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
YER038W-A_d4.405.37E-6YER038W-A_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria

GO enrichment analysis for SGTC_661
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1141.45E-18SGTC_2851486-1325 46.0 μMChemDiv (Drug-like library)223102300.0344828
0.1132.47E-18SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0754717
0.1111.04E-17SGTC_6513253-0966 34.4 μMChemDiv (Drug-like library)7814620.0363636calcium & mitochondrial duress
0.1023.61E-15SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0217391
0.1016.18E-15SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0754717
0.1018.12E-15SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.0425532
0.1001.13E-14SGTC_6681682-0499 62.6 μMChemDiv (Drug-like library)6822140.0357143
0.0983.54E-14SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.0322581
0.0939.14E-13SGTC_1761486-1328 72.8 μMChemDiv (Drug-like library)223102310.0285714
0.0939.52E-13SGTC_24885268135 45.7 μMMiscellaneous12703560.159091
0.0921.57E-12SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0545455mitochondrial processes
0.0912.29E-12SGTC_24785763493 32.3 μMMiscellaneous13655760.142857
0.0912.70E-12SGTC_2717st081029 62.3 μMTimTec (Natural product derivative library)52928460.0483871
0.0881.17E-11SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0166667
0.0871.75E-11SGTC_1076itraconazole 331.0 nMMiscellaneous37930.0416667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8241304-0008100 μM0.636364768245ChemDiv (Drug-like library)236.354762.71606DNA damage response
SGTC_11403229-18482.98 μM0.2142862849525ChemDiv (Drug-like library)254.285542.62105
SGTC_13571503-130339.5 μM0.209302292895ChemDiv (Drug-like library)239.313962.7104
SGTC_10234482-388816 μM0.1914893303168ChemDiv (Drug-like library)267.324062.58205TSC3-RPN4
SGTC_18835-benzylidenerhodanine20 μM0.1751273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_24545-benzylidenerhodanine10.36 μM0.1751273210Miscellaneous221.298682.84113TRP & mitochondrial translation
SGTC_14183966-30992.4 μM0.170732740335ChemDiv (Drug-like library)300.194743.58913
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.1590911241132ChemDiv (Drug-like library)227.32642.79414
SGTC_20995377090177.37 μM0.1590912243829Chembridge (Fragment library)240.2540631.89615
SGTC_2488526813545.7 μM0.1590911270356Miscellaneous247.335963.30913
SGTC_21015377231179.23 μM0.1521742844213Chembridge (Fragment library)256.708662.35514