k832-1615

5-(4-fluorophenyl)-7-(4-methylphenyl)-1,7-dihydro-[1,2,4]triazolo[1,5-a]pyrimidine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_662
Screen concentration 104.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5878478
SMILES CC1=CC=C(C=C1)C2C=C(N=C3N2NC=N3)C4=CC=C(C=C4)F
Standardized SMILES Cc1ccc(cc1)C2C=C(Nc3ncnn23)c4ccc(F)cc4
Molecular weight 306.3369
ALogP 2.76
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.54
% growth inhibition (Hom. pool) 8.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5878478
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.13||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:ESA1(YOR244W)|FD-Score:3.47|P-value:2.64E-4|Clearance:0.18||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:LST8(YNL006W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.02||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:PAN1(YIR006C)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.09||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PRE7(YBL041W)|FD-Score:4.42|P-value:5.03E-6|Clearance:0.28||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP3(YDR473C)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.3||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN9(YDR427W)|FD-Score:3.11|P-value:9.31E-4|Clearance:0.11||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:TAO3(YIL129C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.19||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:YCR013C(YCR013C_d)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YJR141W(YJR141W_p)|FD-Score:3.29|P-value:5.02E-4|Clearance:0.09||SGD DESC:Essential protein of unknown function Gene:YOR282W(YOR282W_d)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:CDC19(YAL038W)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.13||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:ESA1(YOR244W)|FD-Score:3.47|P-value:2.64E-4|Clearance:0.18||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:LST8(YNL006W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.02||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:PAN1(YIR006C)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.09||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:PRE7(YBL041W)|FD-Score:4.42|P-value:5.03E-6|Clearance:0.28||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRP3(YDR473C)|FD-Score:4.72|P-value:1.20E-6|Clearance:0.3||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN9(YDR427W)|FD-Score:3.11|P-value:9.31E-4|Clearance:0.11||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:TAO3(YIL129C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.19||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:YCR013C(YCR013C_d)|FD-Score:4.01|P-value:3.04E-5|Clearance:0.19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant Gene:YJR141W(YJR141W_p)|FD-Score:3.29|P-value:5.02E-4|Clearance:0.09||SGD DESC:Essential protein of unknown function Gene:YOR282W(YOR282W_d)|FD-Score:4.14|P-value:1.75E-5|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5878478
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACB1(YGR037C)|FD-Score:11.4|P-value:1.48E-30||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACE2(YLR131C)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BNA3(YJL060W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:CBR1(YIL043C)|FD-Score:-4.84|P-value:6.40E-7||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CKA2(YOR061W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG1(YGL223C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM4(YPL200W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DAL81(YIR023W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP7(YKR024C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DST1(YGL043W)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:DTR1(YBR180W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EMC4(YGL231C)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EMP47(YFL048C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ERG2(YMR202W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FEN1(YCR034W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GCN3(YKR026C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPA2(YER020W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:HOS1(YPR068C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:ICS2(YBR157C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IGO2(YHR132W-A)|FD-Score:-3.76|P-value:8.48E-5||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INO4(YOL108C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IRE1(YHR079C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:JEM1(YJL073W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KRE28(YDR532C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:MCH4(YOL119C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM38(YOL027C)|FD-Score:-4.87|P-value:5.58E-7||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MFA2(YNL145W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MMM1(YLL006W)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL1(YDR116C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OTU2(YHL013C)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFK2(YMR205C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHD1(YKL043W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PHO90(YJL198W)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PLB3(YOL011W)|FD-Score:-4.6|P-value:2.10E-6||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMS1(YNL082W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRM1(YNL279W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PRM2(YIL037C)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PXA2(YKL188C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RAD51(YER095W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAX2(YLR084C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RRD1(YIL153W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAS5(YOR213C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SFB2(YNL049C)|FD-Score:5.31|P-value:5.36E-8||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SGN1(YIR001C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SHE4(YOR035C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLX5(YDL013W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF12(YNR023W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:STP3(YLR375W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWT1(YOR166C)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TLG2(YOL018C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA10(YLR327C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:14.9|P-value:9.17E-51||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBR1(YGR184C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VBA4(YDR119W_p)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:YBR062C(YBR062C_p)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR241C(YBR241C_p)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL088W(YGL088W_d)|FD-Score:-4.79|P-value:8.54E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YKL075C(YKL075C_p)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKL133C(YKL133C_p)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YKR047W(YKR047W_d)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YMR1(YJR110W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YPK1(YKL126W)|FD-Score:6.01|P-value:9.10E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL038W-A(YPL038W-A_p)|FD-Score:4.78|P-value:8.75E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL071C(YPL071C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AAD4(YDL243C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACB1(YGR037C)|FD-Score:11.4|P-value:1.48E-30||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACE2(YLR131C)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BNA3(YJL060W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:CBR1(YIL043C)|FD-Score:-4.84|P-value:6.40E-7||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CKA2(YOR061W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG1(YGL223C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSM4(YPL200W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:DAL81(YIR023W)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP7(YKR024C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DST1(YGL043W)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:DTR1(YBR180W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EMC4(YGL231C)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EMP47(YFL048C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ERG2(YMR202W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FEN1(YCR034W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:GCN3(YKR026C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPA2(YER020W)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:HOS1(YPR068C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:ICS2(YBR157C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IGO2(YHR132W-A)|FD-Score:-3.76|P-value:8.48E-5||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INO4(YOL108C)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:IRE1(YHR079C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:JEM1(YJL073W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KRE28(YDR532C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:MCH4(YOL119C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDM38(YOL027C)|FD-Score:-4.87|P-value:5.58E-7||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MFA2(YNL145W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MMM1(YLL006W)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRPL1(YDR116C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OTU2(YHL013C)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PFK2(YMR205C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHD1(YKL043W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PHO90(YJL198W)|FD-Score:-3.83|P-value:6.51E-5||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PLB3(YOL011W)|FD-Score:-4.6|P-value:2.10E-6||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMS1(YNL082W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PRM1(YNL279W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PRM2(YIL037C)|FD-Score:3.73|P-value:9.52E-5||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PXA2(YKL188C)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RAD51(YER095W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAX2(YLR084C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RRD1(YIL153W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAS5(YOR213C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SFB2(YNL049C)|FD-Score:5.31|P-value:5.36E-8||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SGN1(YIR001C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Cytoplasmic RNA-binding protein, contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation Gene:SHE4(YOR035C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLX5(YDL013W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNF12(YNR023W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:STP3(YLR375W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SWT1(YOR166C)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:TLG2(YOL018C)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA10(YLR327C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:14.9|P-value:9.17E-51||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBR1(YGR184C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:VBA4(YDR119W_p)|FD-Score:4.32|P-value:7.88E-6||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:YBR062C(YBR062C_p)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR241C(YBR241C_p)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL088W(YGL088W_d)|FD-Score:-4.79|P-value:8.54E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YKL075C(YKL075C_p)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YKL133C(YKL133C_p)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YKR047W(YKR047W_d)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YMR1(YJR110W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YPK1(YKL126W)|FD-Score:6.01|P-value:9.10E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL038W-A(YPL038W-A_p)|FD-Score:4.78|P-value:8.75E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL071C(YPL071C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR473C4.721.20E-60.30PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YBL041W4.425.03E-60.28PRE7Beta 6 subunit of the 20S proteasome
YOR282W_d4.141.75E-50.13YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YCR013C_d4.013.04E-50.19YCR013C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
YAL038W3.826.76E-50.14CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YNL006W3.681.16E-40.02LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YIL129C3.661.26E-40.19TAO3Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
YOR244W3.472.64E-40.18ESA1Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy
YJR141W_p3.295.02E-40.09YJR141W_pEssential protein of unknown function
YIR006C3.206.92E-40.09PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YDR427W3.119.31E-40.11RPN9Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
YKR022C3.000.001350.10NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YOR353C2.900.001890.01SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YJR057W2.890.001920.02CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YMR197C2.870.002070.04VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A14.909.17E-51TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGR037C11.401.48E-30ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YKL126W6.019.10E-10YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YNL049C5.315.36E-8SFB2Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p
YGL223C5.141.41E-7COG1Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YPL038W-A_p4.788.75E-7YPL038W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YIR023W4.741.09E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YJL060W4.731.10E-6BNA3Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
YDR127W4.671.48E-6ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YBR241C_p4.513.26E-6YBR241C_pPutative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene
YOR166C4.473.91E-6SWT1RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
YDR119W_p4.327.88E-6VBA4_pProtein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
YLR131C4.308.49E-6ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YOR213C4.191.39E-5SAS5Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
YOL108C4.191.40E-5INO4Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain

GO enrichment analysis for SGTC_662
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3517.00E-171SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.121212TSC3-RPN4
0.3331.31E-152SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.121212TSC3-RPN4
0.3307.26E-150SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0434783TSC3-RPN4
0.3254.02E-145SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.056338TSC3-RPN4
0.3154.95E-136SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0595238TSC3-RPN4
0.3123.05E-133SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0895522TSC3-RPN4
0.2976.52E-121SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0512821TSC3-RPN4
0.2871.58E-112SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0909091TSC3-RPN4
0.2844.54E-110SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0547945TSC3-RPN4
0.2833.97E-109SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0526316TSC3-RPN4
0.2804.99E-107SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0909091TSC3-RPN4
0.2715.06E-100SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.057971TSC3-RPN4
0.2665.85E-96SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.0434783TSC3-RPN4
0.2651.85E-95SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.116883TSC3-RPN4
0.2632.52E-94SGTC_32379133362 49.5 μMChembridge (Drug-like library)438451370.0813954

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_592k241-007346.5 μM0.3454554230158ChemDiv (Drug-like library)284.3512234.52712
SGTC_5694130-2812160 μM0.224138743552ChemDiv (Drug-like library)279.3298633.01614amide catabolism
SGTC_3097911724649.47 μM0.22077916423892Chembridge (Drug-like library)381.4266432.37917
SGTC_23097664088163.95 μM0.189655704922Chembridge (Fragment library)205.1917231.99626
SGTC_4253825-701713.1 μM0.1884063397440ChemDiv (Drug-like library)329.3702435.78914
SGTC_3257913920632.98 μM0.1884064910041Chembridge (Drug-like library)321.419423.04804
SGTC_3312913333071.43 μM0.187517617324Chembridge (Drug-like library)307.3830233.87914
SGTC_8063966-44555.86 μM0.184615714982ChemDiv (Drug-like library)316.3069633.17425
SGTC_3213912988449.47 μM0.18421117190742Chembridge (Drug-like library)325.3369432.37405
SGTC_270485-000132.62 μM0.1833332054479ChemDiv (Drug-like library)266.360764.32313RPP1 & pyrimidine depletion