1486-0733

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_663
Screen concentration 13.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 722445
SMILES CN(C)CCNC1=C2CCCC2=C(C3=NC4=CC=CC=C4N13)C#N
Standardized SMILES CN(C)CCNc1c2CCCc2c(C#N)c3nc4ccccc4n13
Molecular weight 319.4035
ALogP 3.76
H-bond donor count 1
H-bond acceptor count 4
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.3
% growth inhibition (Hom. pool) 4.2


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 722445
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.11||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP3(YJR065C)|FD-Score:4.61|P-value:1.99E-6|Clearance:0.05||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BRN1(YBL097W)|FD-Score:4.56|P-value:2.56E-6|Clearance:0.11||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC20(YGL116W)|FD-Score:-3.87|P-value:5.53E-5|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:KRE9(YJL174W)|FD-Score:7.66|P-value:9.32E-15|Clearance:3.05||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:LCD1(YDR499W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.1||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.28||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:PRE4(YFR050C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.14||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RHO1(YPR165W)|FD-Score:24.6|P-value:3.72E-134|Clearance:14.03||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:ROT1(YMR200W)|FD-Score:10.6|P-value:1.61E-26|Clearance:2.93||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPN5(YDL147W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.23||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN6(YDL097C)|FD-Score:4.45|P-value:4.25E-6|Clearance:0.08||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SAM50(YNL026W)|FD-Score:4.37|P-value:6.10E-6|Clearance:0.44||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC21(YNL287W)|FD-Score:3.13|P-value:8.74E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPT6(YGR116W)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.05||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:ARC19(YKL013C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.11||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP3(YJR065C)|FD-Score:4.61|P-value:1.99E-6|Clearance:0.05||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BRN1(YBL097W)|FD-Score:4.56|P-value:2.56E-6|Clearance:0.11||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC20(YGL116W)|FD-Score:-3.87|P-value:5.53E-5|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:KRE9(YJL174W)|FD-Score:7.66|P-value:9.32E-15|Clearance:3.05||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:LCD1(YDR499W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.1||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.28||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:PRE4(YFR050C)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.14||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RHO1(YPR165W)|FD-Score:24.6|P-value:3.72E-134|Clearance:14.03||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:ROT1(YMR200W)|FD-Score:10.6|P-value:1.61E-26|Clearance:2.93||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPN5(YDL147W)|FD-Score:3.36|P-value:3.85E-4|Clearance:0.23||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN6(YDL097C)|FD-Score:4.45|P-value:4.25E-6|Clearance:0.08||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SAM50(YNL026W)|FD-Score:4.37|P-value:6.10E-6|Clearance:0.44||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SEC21(YNL287W)|FD-Score:3.13|P-value:8.74E-4|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SPT6(YGR116W)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.05||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 722445
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACK1(YDL203C)|FD-Score:23|P-value:2.50E-117||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ALG12(YNR030W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ANP1(YEL036C)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ARC18(YLR370C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:AST1(YBL069W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG8(YBL078C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCK1(YJL095W)|FD-Score:31|P-value:1.44E-211||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCM1(YGR150C)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CLC1(YGR167W)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNE1(YAL058W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CPR7(YJR032W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CWH41(YGL027C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DEP1(YAL013W)|FD-Score:4.3|P-value:8.54E-6||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG5(YMR238W)|FD-Score:13.4|P-value:1.85E-41||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:EDE1(YBL047C)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELC1(YPL046C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:EMC5(YIL027C)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:END3(YNL084C)|FD-Score:11.4|P-value:2.46E-30||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERD1(YDR414C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:5.32|P-value:5.25E-8||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA4(YMR246W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FET3(YMR058W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FIG4(YNL325C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FLC2(YAL053W)|FD-Score:16.6|P-value:1.68E-62||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP40(YPL222W_p)|FD-Score:4.57|P-value:2.49E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPS1(YLL043W)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FYV8(YGR196C)|FD-Score:20.5|P-value:1.71E-93||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GDB1(YPR184W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET1(YGL020C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GLG2(YJL137C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HCH1(YNL281W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HOC1(YJR075W)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HSV2(YGR223C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HUR1(YGL168W)|FD-Score:5.64|P-value:8.72E-9||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IRC3(YDR332W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:6.07|P-value:6.56E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITR2(YOL103W)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:JIP3(YLR331C_d)|FD-Score:10.7|P-value:8.25E-27||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:KCS1(YDR017C)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KRE1(YNL322C)|FD-Score:9.56|P-value:5.82E-22||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LRS4(YDR439W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MID2(YLR332W)|FD-Score:12.1|P-value:8.17E-34||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MKK1(YOR231W)|FD-Score:6.37|P-value:9.72E-11||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MKK2(YPL140C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MNN10(YDR245W)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN11(YJL183W)|FD-Score:4.85|P-value:6.07E-7||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNN2(YBR015C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSC7(YHR039C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUD1(YBR119W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NPR2(YEL062W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NTO1(YPR031W)|FD-Score:8.58|P-value:4.69E-18||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OPI6(YDL096C_d)|FD-Score:4.83|P-value:6.78E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OST3(YOR085W)|FD-Score:5.06|P-value:2.07E-7||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PAC10(YGR078C)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAD1(YDR538W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PEX11(YOL147C)|FD-Score:5.42|P-value:3.03E-8||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PMT1(YDL095W)|FD-Score:4.29|P-value:8.80E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPM1(YDR435C)|FD-Score:-3.83|P-value:6.35E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PPZ1(YML016C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRX1(YBL064C)|FD-Score:5.47|P-value:2.19E-8||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:RCE1(YMR274C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RGD1(YBR260C)|FD-Score:15.2|P-value:2.97E-52||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RLM1(YPL089C)|FD-Score:5.82|P-value:3.00E-9||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.74|P-value:9.05E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RXT2(YBR095C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC7(YDR389W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SHE4(YOR035C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLA1(YBL007C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:38.6|P-value:0||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOP4(YJL192C)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SSD1(YDR293C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSZ1(YHR064C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STM1(YLR150W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SUL2(YLR092W)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SVL3(YPL032C)|FD-Score:-3.84|P-value:6.04E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWA2(YDR320C)|FD-Score:8.94|P-value:1.98E-19||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SXM1(YDR395W)|FD-Score:5|P-value:2.79E-7||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYT1(YPR095C)|FD-Score:4.89|P-value:4.98E-7||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TAT1(YBR069C)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRS65(YGR166W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:USV1(YPL230W)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:UTH1(YKR042W)|FD-Score:11.9|P-value:8.79E-33||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAL056C-A(YAL056C-A_d)|FD-Score:11.1|P-value:4.49E-29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR139W(YBR139W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL041C(YGL041C_d)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:-5.03|P-value:2.39E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGL214W(YGL214W_d)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR242W(YGR242W_d)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YJR018W(YJR018W_d)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLL017W(YLL017W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:YML094C-A(YML094C-A_d)|FD-Score:6.03|P-value:8.20E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR252C(YMR252C_p)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL089C(YNL089C_d)|FD-Score:7.93|P-value:1.11E-15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNL324W(YNL324W_d)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR092W(YPR092W_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YUR1(YJL139C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:AAT2(YLR027C)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACK1(YDL203C)|FD-Score:23|P-value:2.50E-117||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ALG12(YNR030W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:ANP1(YEL036C)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ARC18(YLR370C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:AST1(YBL069W)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATG8(YBL078C)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCK1(YJL095W)|FD-Score:31|P-value:1.44E-211||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCM1(YGR150C)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CLC1(YGR167W)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CNE1(YAL058W)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:CPR7(YJR032W)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CWH41(YGL027C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DEP1(YAL013W)|FD-Score:4.3|P-value:8.54E-6||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG5(YMR238W)|FD-Score:13.4|P-value:1.85E-41||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:EDE1(YBL047C)|FD-Score:5.11|P-value:1.58E-7||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELC1(YPL046C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:EMC5(YIL027C)|FD-Score:-3.34|P-value:4.25E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:END3(YNL084C)|FD-Score:11.4|P-value:2.46E-30||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERD1(YDR414C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERG4(YGL012W)|FD-Score:5.32|P-value:5.25E-8||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA4(YMR246W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FET3(YMR058W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:FIG4(YNL325C)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FLC2(YAL053W)|FD-Score:16.6|P-value:1.68E-62||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FMP40(YPL222W_p)|FD-Score:4.57|P-value:2.49E-6||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FPS1(YLL043W)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FYV8(YGR196C)|FD-Score:20.5|P-value:1.71E-93||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GDB1(YPR184W)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET1(YGL020C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GLG2(YJL137C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HCH1(YNL281W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HOC1(YJR075W)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HSV2(YGR223C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HUR1(YGL168W)|FD-Score:5.64|P-value:8.72E-9||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IRC3(YDR332W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:6.07|P-value:6.56E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITR2(YOL103W)|FD-Score:3.91|P-value:4.53E-5||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:JIP3(YLR331C_d)|FD-Score:10.7|P-value:8.25E-27||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:KCS1(YDR017C)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KRE1(YNL322C)|FD-Score:9.56|P-value:5.82E-22||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LRS4(YDR439W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MID2(YLR332W)|FD-Score:12.1|P-value:8.17E-34||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MKK1(YOR231W)|FD-Score:6.37|P-value:9.72E-11||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication Gene:MKK2(YPL140C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MNN10(YDR245W)|FD-Score:5.89|P-value:1.98E-9||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN11(YJL183W)|FD-Score:4.85|P-value:6.07E-7||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MNN2(YBR015C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSC7(YHR039C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MUD1(YBR119W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NPR2(YEL062W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:NTO1(YPR031W)|FD-Score:8.58|P-value:4.69E-18||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OPI6(YDL096C_d)|FD-Score:4.83|P-value:6.78E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OST3(YOR085W)|FD-Score:5.06|P-value:2.07E-7||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PAC10(YGR078C)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAD1(YDR538W)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PEX11(YOL147C)|FD-Score:5.42|P-value:3.03E-8||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PMT1(YDL095W)|FD-Score:4.29|P-value:8.80E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPM1(YDR435C)|FD-Score:-3.83|P-value:6.35E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PPZ1(YML016C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRX1(YBL064C)|FD-Score:5.47|P-value:2.19E-8||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:RCE1(YMR274C)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RGD1(YBR260C)|FD-Score:15.2|P-value:2.97E-52||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RLM1(YPL089C)|FD-Score:5.82|P-value:3.00E-9||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.74|P-value:9.05E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RXT2(YBR095C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC7(YDR389W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SHE4(YOR035C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLA1(YBL007C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:5.51|P-value:1.81E-8||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:38.6|P-value:0||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SOP4(YJL192C)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SSD1(YDR293C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSZ1(YHR064C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP Gene:STM1(YLR150W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SUL2(YLR092W)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SVL3(YPL032C)|FD-Score:-3.84|P-value:6.04E-5||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWA2(YDR320C)|FD-Score:8.94|P-value:1.98E-19||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SXM1(YDR395W)|FD-Score:5|P-value:2.79E-7||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYT1(YPR095C)|FD-Score:4.89|P-value:4.98E-7||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TAT1(YBR069C)|FD-Score:-3.88|P-value:5.28E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRS65(YGR166W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:USV1(YPL230W)|FD-Score:-4.01|P-value:2.99E-5||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:UTH1(YKR042W)|FD-Score:11.9|P-value:8.79E-33||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YAL056C-A(YAL056C-A_d)|FD-Score:11.1|P-value:4.49E-29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR139W(YBR139W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL041C(YGL041C_d)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL088W(YGL088W_d)|FD-Score:-5.03|P-value:2.39E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGL214W(YGL214W_d)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR242W(YGR242W_d)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YJR018W(YJR018W_d)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLL017W(YLL017W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:YML094C-A(YML094C-A_d)|FD-Score:6.03|P-value:8.20E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR252C(YMR252C_p)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:3.82|P-value:6.70E-5||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL089C(YNL089C_d)|FD-Score:7.93|P-value:1.11E-15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YNL324W(YNL324W_d)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR092W(YPR092W_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YUR1(YJL139C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W24.603.72E-13414.00RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YMR200W10.601.61E-262.93ROT1Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
YJL174W7.669.32E-153.05KRE9Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
YJR065C4.611.99E-60.05ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YBL097W4.562.56E-60.11BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YDL097C4.454.25E-60.08RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YNL026W4.376.10E-60.44SAM50Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
YGR116W3.944.15E-50.05SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YLR163C3.895.11E-50.28MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YFR050C3.601.58E-40.14PRE4Beta 7 subunit of the 20S proteasome
YDR499W3.462.68E-40.10LCD1Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
YDL147W3.363.85E-40.23RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YNL287W3.138.74E-40.00SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YKL013C3.138.85E-40.11ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YDR429C3.020.001280.06TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR030C38.600SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YJL095W31.001.44E-211BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YDL203C23.002.50E-117ACK1Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
YGR196C20.501.71E-93FYV8Protein of unknown function, required for survival upon exposure to K1 killer toxin
YAL053W16.601.68E-62FLC2Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
YBR260C15.202.97E-52RGD1GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
YMR238W13.401.85E-41DFG5Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
YLR332W12.108.17E-34MID2O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
YKR042W11.908.79E-33UTH1Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
YNL084C11.402.46E-30END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YAL056C-A_d11.104.49E-29YAL056C-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR331C_d10.708.25E-27JIP3_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
YNL322C9.565.82E-22KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YDR320C8.941.98E-19SWA2Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
YPR031W8.584.69E-18NTO1Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3

GO enrichment analysis for SGTC_663
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.7140SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.109589cell wall signaling
0.6480SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.0933333cell wall signaling
0.6010SGTC_23995884133 200.0 μMMiscellaneous7224451cell wall signaling
0.5840SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.180556cell wall signaling
0.4630SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0729167cell wall
0.4582.36E-304SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.238806cell wall signaling
0.4357.36E-271SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0909091cell wall signaling
0.4341.19E-269SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.0555556cell wall signaling
0.4283.17E-262SGTC_500h-89 112.0 μMICCB bioactive library4492410.0879121cell wall signaling
0.4154.43E-245SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.091954cell wall signaling
0.4028.51E-228SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.121212cell wall signaling
0.3991.76E-224SGTC_422cantharidin 155.0 μMICCB bioactive library25450.0447761cell wall signaling
0.3945.28E-219SGTC_31229126309 49.5 μMChembridge (Drug-like library)175912060.0941176cell wall signaling
0.3473.25E-167SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.101266cell wall
0.3401.91E-159SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0724638cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23995884133200 μM1722445Miscellaneous319.403543.75814cell wall signaling
SGTC_3353915164439.28 μM0.3333334909158Chembridge (Drug-like library)309.408722.87614
SGTC_23266164455177.58 μM0.2647062888790Chembridge (Fragment library)260.761883.88722mitochondrial processes
SGTC_9011112-006260.1 μM0.2575761908457ChemDiv (Drug-like library)224.300943.69101
SGTC_14534237-0541195 μM0.257143739892ChemDiv (Drug-like library)277.32053.43623heme biosynthesis & mitochondrial translocase
SGTC_6023130-153338.1 μM0.246377720941ChemDiv (Drug-like library)274.359625.11612
SGTC_8291635-0120117 μM0.245902285054ChemDiv (Drug-like library)220.229422.38903DNA damage response
SGTC_21495554786194.83 μM0.238806745063Chembridge (Fragment library)230.308821.94613cell wall signaling
SGTC_8860866-0150153 μM0.234375271463ChemDiv (Drug-like library)199.248423.14201
SGTC_14504112-426037.6 μM0.2337661633175ChemDiv (Drug-like library)335.44274.06714calcium & mitochondrial duress