3914-0008

4-[4-(6-methyl-4-phenylquinazolin-2-yl)piperazin-1-yl]-4-oxobutanoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_665
Screen concentration 307.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1069549
SMILES CC1=CC2=C(C=C1)N=C(N=C2C3=CC=CC=C3)N4CCN(CC4)C(=O)CCC(=O)O
Standardized SMILES Cc1ccc2nc(nc(c3ccccc3)c2c1)N4CCN(CC4)C(=O)CCC(=O)O
Molecular weight 404.4617
ALogP 3.57
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.41
% growth inhibition (Hom. pool) 3.35


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1069549
Download HIP data (tab-delimited text)  (excel)
Gene:CAB4(YGR277C)|FD-Score:3.11|P-value:9.41E-4|Clearance:0.08||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:KAP95(YLR347C)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.09||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LSG1(YGL099W)|FD-Score:9.85|P-value:3.31E-23|Clearance:5.61||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.35||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUS1(YDL193W)|FD-Score:3.89|P-value:5.06E-5|Clearance:0.36||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PDS5(YMR076C)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.01||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:POL2(YNL262W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.22||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PTR3(YFR029W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.05||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RPL25(YOL127W)|FD-Score:-3.74|P-value:9.26E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RSA4(YCR072C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.05||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SDA1(YGR245C)|FD-Score:-3.58|P-value:1.75E-4|Clearance:0||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis Gene:CAB4(YGR277C)|FD-Score:3.11|P-value:9.41E-4|Clearance:0.08||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:KAP95(YLR347C)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.09||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:LSG1(YGL099W)|FD-Score:9.85|P-value:3.31E-23|Clearance:5.61||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NMD3(YHR170W)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.35||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUS1(YDL193W)|FD-Score:3.89|P-value:5.06E-5|Clearance:0.36||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PDS5(YMR076C)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.01||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:POL2(YNL262W)|FD-Score:3.33|P-value:4.40E-4|Clearance:0.22||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PTR3(YFR029W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.05||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RPL25(YOL127W)|FD-Score:-3.74|P-value:9.26E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RSA4(YCR072C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.05||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:SDA1(YGR245C)|FD-Score:-3.58|P-value:1.75E-4|Clearance:0||SGD DESC:Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1069549
Download HOP data (tab-delimited text)  (excel)
Gene:ARO10(YDR380W)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ARO80(YDR421W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:BRE5(YNR051C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BUL2(YML111W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CBP1(YJL209W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CHS7(YHR142W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG5(YNL051W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX11(YPL132W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CTH1(YDR151C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DCR2(YLR361C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START Gene:DIA1(YMR316W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DPP1(YDR284C)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DYN1(YKR054C)|FD-Score:-3.73|P-value:9.72E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:EMC3(YKL207W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FAR11(YNL127W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FCY1(YPR062W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FTR1(YER145C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GAP1(YKR039W)|FD-Score:-5.52|P-value:1.72E-8||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCN5(YGR252W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HNT3(YOR258W)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:INM1(YHR046C)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:ITR1(YDR497C)|FD-Score:-5.18|P-value:1.09E-7||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KIN4(YOR233W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LOH1(YJL038C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:LSB3(YFR024C-A)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MOG1(YJR074W)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL1(YDR116C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC6(YOR354C)|FD-Score:4.8|P-value:8.10E-7||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NHA1(YLR138W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:PAL1(YDR348C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAT1(YCR077C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGC1(YPL206C)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO8(YDR481C)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PKH1(YDR490C)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PRY1(YJL079C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RGA2(YDR379W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIP1(YEL024W)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RKM4(YDR257C)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RPS11B(YBR048W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSA1(YPL193W)|FD-Score:5.34|P-value:4.62E-8||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RTR2(YDR066C_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:9.5|P-value:1.08E-21||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAM37(YMR060C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SIP4(YJL089W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLX5(YDL013W)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SRN2(YLR119W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:STE13(YOR219C)|FD-Score:-4.46|P-value:4.10E-6||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STI1(YOR027W)|FD-Score:4.31|P-value:8.13E-6||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TDA11(YHR159W_p)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TGS1(YPL157W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOP1(YOL006C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:UTH1(YKR042W)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS61(YDR136C_d)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAK1(YJL141C)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YDR391C(YDR391C_p)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR476C(YDR476C_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YER121W(YER121W_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YER134C(YER134C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YHL045W(YHL045W_d)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL215C(YJL215C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR098C(YJR098C_p)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR078W(YKR078W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR419W(YLR419W_p)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML009W-B(YML009W-B_d)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR057C(YMR057C_d)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR160W(YMR160W_p)|FD-Score:5.34|P-value:4.53E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNL226W(YNL226W_d)|FD-Score:4.27|P-value:9.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR053W(YOR053W_d)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR072W(YOR072W_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR292C(YOR292C_p)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPR092W(YPR092W_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ARO10(YDR380W)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Phenylpyruvate decarboxylase, catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway Gene:ARO80(YDR421W)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:BRE5(YNR051C)|FD-Score:3.87|P-value:5.54E-5||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BUL2(YML111W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Component of the Rsp5p E3-ubiquitin ligase complex; involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication Gene:CBP1(YJL209W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CHS7(YHR142W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG5(YNL051W)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX11(YPL132W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:CTH1(YDR151C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DCR2(YLR361C)|FD-Score:3.13|P-value:8.89E-4||SGD DESC:Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START Gene:DIA1(YMR316W)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DPP1(YDR284C)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DYN1(YKR054C)|FD-Score:-3.73|P-value:9.72E-5||SGD DESC:Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p Gene:EMC3(YKL207W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FAR11(YNL127W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FCY1(YPR062W)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FTR1(YER145C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GAP1(YKR039W)|FD-Score:-5.52|P-value:1.72E-8||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCN5(YGR252W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HNT3(YOR258W)|FD-Score:5.12|P-value:1.52E-7||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:INM1(YHR046C)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:ITR1(YDR497C)|FD-Score:-5.18|P-value:1.09E-7||SGD DESC:Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress Gene:KIN4(YOR233W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:LOH1(YJL038C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:LSB3(YFR024C-A)|FD-Score:-4.24|P-value:1.12E-5||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MOG1(YJR074W)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL1(YDR116C)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC6(YOR354C)|FD-Score:4.8|P-value:8.10E-7||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NHA1(YLR138W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:PAL1(YDR348C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PAT1(YCR077C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGC1(YPL206C)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs Gene:PHO8(YDR481C)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PKH1(YDR490C)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PRY1(YJL079C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:RGA2(YDR379W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RIP1(YEL024W)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:RKM4(YDR257C)|FD-Score:-3.85|P-value:5.84E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RPS11B(YBR048W)|FD-Score:4.51|P-value:3.26E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSA1(YPL193W)|FD-Score:5.34|P-value:4.62E-8||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RTR2(YDR066C_p)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:9.5|P-value:1.08E-21||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAM37(YMR060C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SIP4(YJL089W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SLX5(YDL013W)|FD-Score:3.12|P-value:8.92E-4||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SRN2(YLR119W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:STE13(YOR219C)|FD-Score:-4.46|P-value:4.10E-6||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STI1(YOR027W)|FD-Score:4.31|P-value:8.13E-6||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TDA11(YHR159W_p)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TGS1(YPL157W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TOP1(YOL006C)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination Gene:UTH1(YKR042W)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VPS61(YDR136C_d)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAK1(YJL141C)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YDR391C(YDR391C_p)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR476C(YDR476C_p)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YER121W(YER121W_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YER134C(YER134C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YHL045W(YHL045W_d)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL215C(YJL215C_d)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR098C(YJR098C_p)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YKR078W(YKR078W)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Cytoplasmic protein of unknown function, has similarity to Vps5p; potential Cdc28p substrate; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:YLR419W(YLR419W_p)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YML009W-B(YML009W-B_d)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YMR057C(YMR057C_d)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1 Gene:YMR160W(YMR160W_p)|FD-Score:5.34|P-value:4.53E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNL226W(YNL226W_d)|FD-Score:4.27|P-value:9.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR053W(YOR053W_d)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR072W(YOR072W_d)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR292C(YOR292C_p)|FD-Score:-4.25|P-value:1.06E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:YPR092W(YPR092W_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W9.853.31E-235.61LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W4.241.11E-50.35NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDL193W3.895.06E-50.36NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YFR029W3.532.09E-40.05PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YCR072C3.482.53E-40.05RSA4WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
YLR347C3.423.08E-40.09KAP95Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
YMR076C3.334.29E-40.01PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YNL262W3.334.40E-40.22POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YGR277C3.119.41E-40.08CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YMR047C3.030.001240.07NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YBR153W2.960.001560.01RIB7Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
YML010W2.950.001600.06SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YBR234C2.890.001920.00ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YKR022C2.890.001950.02NTR2Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
YIL046W2.870.002070.01MET30F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL212W9.501.08E-21SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YMR160W_p5.344.53E-8YMR160W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress
YPL193W5.344.62E-8RSA1Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
YOR053W_d5.171.16E-7YOR053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
YIL153W5.161.24E-7RRD1Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YOR258W5.121.52E-7HNT3DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
YJL141C4.875.63E-7YAK1Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose
YOR354C4.808.10E-7MSC6Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YHL045W_d4.691.38E-6YHL045W_dPutative protein of unknown function; not an essential gene
YLR119W4.542.85E-6SRN2Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
YBR048W4.513.26E-6RPS11BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
YEL024W4.503.37E-6RIP1Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
YOR027W4.318.13E-6STI1Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
YNL226W_d4.279.73E-6YNL226W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
YOR072W_d4.241.12E-5YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive

GO enrichment analysis for SGTC_665
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2017.87E-55SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.10526360S ribosome export
0.1976.20E-53SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.12371160S ribosome export
0.1859.60E-47SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.112560S ribosome export
0.1688.15E-39SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.212560S ribosome export
0.1645.68E-37SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.17721560S ribosome export
0.1646.23E-37SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.29577560S ribosome export
0.1625.06E-36SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.12222260S ribosome export
0.1453.61E-29SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.10465160S ribosome export
0.1404.52E-27SGTC_32809140961 49.5 μMChembridge (Drug-like library)49012260.18518560S ribosome export
0.1372.70E-26SGTC_29379044753 11.3 μMChembridge (Drug-like library)64655300.10112460S ribosome export
0.1343.40E-25SGTC_5794491-1009 110.0 μMChemDiv (Drug-like library)12710410.16438460S ribosome export
0.1321.66E-24SGTC_1733st037357 54.9 μMTimTec (Natural product derivative library)12723390.12643760S ribosome export
0.1315.61E-24SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.11538560S ribosome export
0.1279.00E-23SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.16216260S ribosome export
0.1271.07E-22SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.11538560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12063978-0017132 μM0.7894743873677ChemDiv (Drug-like library)469.331143.82816ERG2
SGTC_6133914-0030117 μM0.388061145395ChemDiv (Drug-like library)358.189383.89425
SGTC_11513909-831615.3 μM0.3800431ChemDiv (Drug-like library)332.442064.50104
SGTC_21856113692200 μM0.296875874150Chembridge (Fragment library)250.3118632.53703ergosterol biosynthesis
SGTC_1913914-012366.51 μM0.2957753144412ChemDiv (Drug-like library)358.232443.9362460S ribosome export
SGTC_23829035899200 μM0.2941186462679Chembridge (Fragment library)288.368041.9431460S ribosome export
SGTC_2376907292281.32 μM0.28985517087968Chembridge (Fragment library)281.78112.2513
SGTC_2970909093841.86 μM0.28947417217104Chembridge (Drug-like library)339.43143.55304RNA processing & uracil transport
SGTC_3214534-389065.3 μM0.285714699746ChemDiv (Drug-like library)345.82333.24704RPP1 & pyrimidine depletion
SGTC_12931000-1278195 μM0.285714678847ChemDiv (Drug-like library)342.186683.16912