1498-1044

2-[(2-oxonaphthalen-1-ylidene)methylamino]benzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_667
Screen concentration 59.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6739982
SMILES C1=CC=C2C(=C1)C=CC(=O)C2=CNC3=CC=CC=C3C(=O)O
Standardized SMILES OC(=O)c1ccccc1N=Cc2c(O)ccc3ccccc23
Molecular weight 291.3007
ALogP 2.78
H-bond donor count 2
H-bond acceptor count 4
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.89
% growth inhibition (Hom. pool) 7.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6739982
Download HIP data (tab-delimited text)  (excel)
Gene:ACP1(YKL192C)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:AOS1(YPR180W)|FD-Score:3.31|P-value:4.66E-4|Clearance:0.04||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CWC22(YGR278W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:GCD11(YER025W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LIP1(YMR298W)|FD-Score:-4.08|P-value:2.28E-5|Clearance:0||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:POP4(YBR257W)|FD-Score:3.28|P-value:5.28E-4|Clearance:0.23||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRE4(YFR050C)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.05||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPC25(YKL144C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPC53(YDL150W)|FD-Score:3.33|P-value:4.37E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.06||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:9.77|P-value:7.47E-23|Clearance:3.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:4.76|P-value:9.59E-7|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC58(YLR033W)|FD-Score:3.79|P-value:7.66E-5|Clearance:0.18||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC31(YDL195W)|FD-Score:5.32|P-value:5.13E-8|Clearance:0.3||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMD2(YLR275W)|FD-Score:6.58|P-value:2.33E-11|Clearance:1.26||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SPP382(YLR424W)|FD-Score:5.03|P-value:2.51E-7|Clearance:0.26||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SUI3(YPL237W)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.47||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF35(YDR429C)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.39||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:ACP1(YKL192C)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p Gene:AOS1(YPR180W)|FD-Score:3.31|P-value:4.66E-4|Clearance:0.04||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CWC22(YGR278W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:GCD11(YER025W)|FD-Score:3.57|P-value:1.75E-4|Clearance:0.14||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GLC7(YER133W)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LIP1(YMR298W)|FD-Score:-4.08|P-value:2.28E-5|Clearance:0||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:POP4(YBR257W)|FD-Score:3.28|P-value:5.28E-4|Clearance:0.23||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRE4(YFR050C)|FD-Score:4.23|P-value:1.17E-5|Clearance:0.05||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPC25(YKL144C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p Gene:RPC53(YDL150W)|FD-Score:3.33|P-value:4.37E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.06||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:9.77|P-value:7.47E-23|Clearance:3.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:4.76|P-value:9.59E-7|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC58(YLR033W)|FD-Score:3.79|P-value:7.66E-5|Clearance:0.18||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEC31(YDL195W)|FD-Score:5.32|P-value:5.13E-8|Clearance:0.3||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMD2(YLR275W)|FD-Score:6.58|P-value:2.33E-11|Clearance:1.26||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SPP382(YLR424W)|FD-Score:5.03|P-value:2.51E-7|Clearance:0.26||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SUI3(YPL237W)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.47||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TIF35(YDR429C)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.39||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6739982
Download HOP data (tab-delimited text)  (excel)
Gene:AIM44(YPL158C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALB1(YJL122W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARO3(YDR035W)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:AVT1(YJR001W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BOR1(YNL275W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BRE1(YDL074C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CAT8(YMR280C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CHA4(YLR098C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:COX7(YMR256C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYB2(YML054C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAL81(YIR023W)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCN1(YLR128W)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DTD1(YDL219W)|FD-Score:-5.51|P-value:1.81E-8||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EAF1(YDR359C)|FD-Score:5.29|P-value:6.24E-8||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ELP4(YPL101W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG2(YMR202W)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EST3(YIL009C-A)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FAA4(YMR246W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FUS2(YMR232W)|FD-Score:5.14|P-value:1.37E-7||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GCN4(YEL009C)|FD-Score:-4.51|P-value:3.24E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCN5(YGR252W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GET2(YER083C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLC8(YMR311C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GOS1(YHL031C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GRX2(YDR513W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:GVP36(YIL041W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:ICE2(YIL090W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IKI1(YHR187W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:INM1(YHR046C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRC2(YDR112W_d)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:4.76|P-value:9.78E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAT1(YNL071W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LST4(YKL176C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRPL16(YBL038W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC1(YML128C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MTC6(YHR151C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MUS81(YDR386W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MVB12(YGR206W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:PDC1(YLR044C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PDR16(YNL231C)|FD-Score:-5.44|P-value:2.64E-8||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX18(YHR160C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PLM2(YDR501W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication Gene:PMR1(YGL167C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:RCE1(YMR274C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RQC1(YDR333C_p)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RRP6(YOR001W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:RRT1(YBL048W_d)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SAP155(YFR040W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SMM1(YNR015W)|FD-Score:-5.14|P-value:1.39E-7||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPT2(YER161C)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRO9(YCL037C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.3|P-value:8.65E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP2(YER090W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUS1(YLR425W)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA3(YPR066W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMA8(YEL051W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS61(YDR136C_d)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL059W(YBL059W_p)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YDR010C(YDR010C_d)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR442W(YDR442W_d)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR541C(YDR541C_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER175W-A(YER175W-A_p)|FD-Score:7.54|P-value:2.33E-14||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL117W(YGL117W_p)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function Gene:YGR045C(YGR045C_d)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR266W(YGR266W)|FD-Score:-4.84|P-value:6.55E-7||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHL045W(YHL045W_d)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR202W(YHR202W_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJR149W(YJR149W_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL059C(YLL059C_d)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR063W(YLR063W_p)|FD-Score:-3.85|P-value:5.97E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNR021W(YNR021W_p)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YPL264C(YPL264C_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR011C(YPR011C_p)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR015C(YPR015C_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPS1(YLR120C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSC83(YHR017W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AIM44(YPL158C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALB1(YJL122W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ARO3(YDR035W)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:AVT1(YJR001W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BOR1(YNL275W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BRE1(YDL074C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:CAT8(YMR280C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress Gene:CHA4(YLR098C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:COX7(YMR256C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYB2(YML054C)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAL81(YIR023W)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCN1(YLR128W)|FD-Score:4.43|P-value:4.80E-6||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DTD1(YDL219W)|FD-Score:-5.51|P-value:1.81E-8||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EAF1(YDR359C)|FD-Score:5.29|P-value:6.24E-8||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ELP4(YPL101W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG2(YMR202W)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:EST3(YIL009C-A)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FAA4(YMR246W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FUS2(YMR232W)|FD-Score:5.14|P-value:1.37E-7||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GCN4(YEL009C)|FD-Score:-4.51|P-value:3.24E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCN5(YGR252W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GET2(YER083C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLC8(YMR311C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GOS1(YHL031C)|FD-Score:3.21|P-value:6.60E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GRX2(YDR513W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:GVP36(YIL041W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:ICE2(YIL090W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IKI1(YHR187W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:INM1(YHR046C)|FD-Score:-3.41|P-value:3.22E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRC2(YDR112W_d)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:4.76|P-value:9.78E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAT1(YNL071W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LST4(YKL176C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRPL16(YBL038W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC1(YML128C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:MTC6(YHR151C)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MUS81(YDR386W)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:MVB12(YGR206W)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:PDC1(YLR044C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PDR16(YNL231C)|FD-Score:-5.44|P-value:2.64E-8||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX18(YHR160C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PLM2(YDR501W)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication Gene:PMR1(YGL167C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:RCE1(YMR274C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RQC1(YDR333C_p)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RRP6(YOR001W)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:RRT1(YBL048W_d)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SAP155(YFR040W)|FD-Score:3.77|P-value:8.12E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SMM1(YNR015W)|FD-Score:-5.14|P-value:1.39E-7||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SPT2(YER161C)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRO9(YCL037C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.3|P-value:8.65E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP2(YER090W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUS1(YLR425W)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA3(YPR066W)|FD-Score:3.98|P-value:3.40E-5||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VMA8(YEL051W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS61(YDR136C_d)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:4.89|P-value:4.93E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL059W(YBL059W_p)|FD-Score:4.08|P-value:2.28E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YDR010C(YDR010C_d)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR442W(YDR442W_d)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR541C(YDR541C_p)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER175W-A(YER175W-A_p)|FD-Score:7.54|P-value:2.33E-14||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL117W(YGL117W_p)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Putative protein of unknown function Gene:YGR045C(YGR045C_d)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR204C-A(YGR204C-A_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGR266W(YGR266W)|FD-Score:-4.84|P-value:6.55E-7||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHL045W(YHL045W_d)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR202W(YHR202W_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJR149W(YJR149W_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL059C(YLL059C_d)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR063W(YLR063W_p)|FD-Score:-3.85|P-value:5.97E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YMR315W(YMR315W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNR021W(YNR021W_p)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YPL264C(YPL264C_p)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR011C(YPR011C_p)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR015C(YPR015C_p)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPS1(YLR120C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YSC83(YHR017W)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C9.777.47E-233.19RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR275W6.582.33E-111.26SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YDL195W5.325.13E-80.30SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YLR424W5.032.51E-70.26SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YHR069C4.769.59E-70.06RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YPL237W4.701.32E-60.47SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YFR050C4.231.17E-50.05PRE4Beta 7 subunit of the 20S proteasome
YDR429C4.181.47E-50.39TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YLR033W3.797.66E-50.18RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YKL144C3.611.55E-40.03RPC25RNA polymerase III subunit C25, required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
YER025W3.571.75E-40.14GCD11Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
YER133W3.432.98E-40.04GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YOR116C3.393.46E-40.06RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YDL150W3.334.37E-40.02RPC53RNA polymerase III subunit C53
YPR180W3.314.66E-40.04AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER175W-A_p7.542.33E-14YER175W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YBL048W_d6.311.40E-10RRT1_dIdentified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YDR359C5.296.24E-8EAF1Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
YMR232W5.141.37E-7FUS2Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating
YIR023W4.943.86E-7DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YLR261C_d4.894.93E-7VPS63_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
YKR019C4.769.78E-7IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YIL009C-A4.661.61E-6EST3Component of the telomerase holoenzyme, involved in telomere replication
YLR128W4.434.80E-6DCN1Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation
YDR136C_d4.318.30E-6VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
YOL018C4.308.65E-6TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YPL158C4.251.08E-5AIM44Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBL059W_p4.082.28E-5YBL059W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
YHR151C4.082.28E-5MTC6Protein of unknown function; mtc6 is synthetically sick with cdc13-1
YKL176C4.052.58E-5LST4Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface

GO enrichment analysis for SGTC_667
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1775.57E-43SGTC_8282074-0507 139.0 μMChemDiv (Drug-like library)X8280.375RPP1 & pyrimidine depletion
0.1735.97E-41SGTC_1320417-1715 25.8 μMChemDiv (Drug-like library)67690980.242424RPP1 & pyrimidine depletion
0.1739.76E-41SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.211268
0.1619.78E-36SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.1521.16E-31SGTC_2023594-0513 34.8 μMChemDiv (Drug-like library)7084290.140351PDR1
0.1411.34E-27SGTC_13993562-5772 25.0 μMChemDiv (Drug-like library)238474900.106667RPP1 & pyrimidine depletion
0.1411.32E-27SGTC_2686alizarin 95.4 μMMiscellaneous62930.196078RPP1 & pyrimidine depletion
0.1396.04E-27SGTC_13271436-0082 2.2 μMChemDiv (Drug-like library)159922050.126984
0.1381.31E-26SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.1
0.1352.99E-25SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.112676amide catabolism
0.1345.29E-25SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0512821RPP1 & pyrimidine depletion
0.1321.95E-24SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.173077copper-dependent oxidative stress
0.1315.51E-24SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.0434783
0.1284.23E-23SGTC_486niguldipine 82.0 μMMiscellaneous12360.0842105amide catabolism
0.1285.37E-23SGTC_389tpck 16.7 μMMiscellaneous4396470.117647

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7851270-011165.3 μM0.6888896741994ChemDiv (Drug-like library)307.300122.53735
SGTC_8981000-084928.3 μM0.5098046751807ChemDiv (Drug-like library)356.374042.72334
SGTC_780336-0123156.75 μM0.4909096765896ChemDiv (Drug-like library)380.461645.15814
SGTC_6441498-119763.3 μM0.486741615ChemDiv (Drug-like library)291.343782.86623
SGTC_7181130-0073349 μM0.4705886740020ChemDiv (Drug-like library)282.724343.20213
SGTC_1280906-298113.37 μM0.4629636740226ChemDiv (Drug-like library)296.343682.0124
SGTC_11760848-013183.3 μM0.406786769040ChemDiv (Drug-like library)414.454725.58514Golgi
SGTC_2235salicylic acid500 μM0.4338Miscellaneous138.120741.21723
SGTC_3740880-023182.3 μM0.3968256833587ChemDiv (Drug-like library)382.43453.24523iron homeostasis
SGTC_12800880-025310.1 μM0.3968256836607ChemDiv (Drug-like library)410.44462.91724