1682-0499

2-(2,4-dichlorophenoxy)-N-(2-phenylethyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_668
Screen concentration 62.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 682214
SMILES C1=CC=C(C=C1)CCNC(=O)COC2=C(C=C(C=C2)Cl)Cl
Standardized SMILES Clc1ccc(OCC(=O)NCCc2ccccc2)c(Cl)c1
Molecular weight 324.2018
ALogP 4.11
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.89
% growth inhibition (Hom. pool) 8.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 682214
Download HIP data (tab-delimited text)  (excel)
Gene:AUR1(YKL004W)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.61||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:CDC13(YDL220C)|FD-Score:3.73|P-value:9.51E-5|Clearance:0.16||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:DHR2(YKL078W)|FD-Score:4.94|P-value:3.86E-7|Clearance:0.61||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF1(YDR339C)|FD-Score:-5.28|P-value:6.48E-8|Clearance:0||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GAA1(YLR088W)|FD-Score:3.11|P-value:9.48E-4|Clearance:0.05||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GPI13(YLL031C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.01||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:LTO1(YNL260C_p)|FD-Score:-3.28|P-value:5.10E-4|Clearance:0||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MCM2(YBL023C)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.61||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:NOP14(YDL148C)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.22||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RIO2(YNL207W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.37||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RRP5(YMR229C)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:TFC1(YBR123C)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:YLR076C(YLR076C_d)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:AUR1(YKL004W)|FD-Score:5.18|P-value:1.09E-7|Clearance:0.61||SGD DESC:Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance Gene:CDC13(YDL220C)|FD-Score:3.73|P-value:9.51E-5|Clearance:0.16||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:DHR2(YKL078W)|FD-Score:4.94|P-value:3.86E-7|Clearance:0.61||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:FCF1(YDR339C)|FD-Score:-5.28|P-value:6.48E-8|Clearance:0||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GAA1(YLR088W)|FD-Score:3.11|P-value:9.48E-4|Clearance:0.05||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GPI13(YLL031C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.01||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:LTO1(YNL260C_p)|FD-Score:-3.28|P-value:5.10E-4|Clearance:0||SGD DESC:Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:MCM2(YBL023C)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.61||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:NOP14(YDL148C)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.22||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:RIO2(YNL207W)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.37||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RRP5(YMR229C)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress Gene:TFC1(YBR123C)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:YLR076C(YLR076C_d)|FD-Score:3.19|P-value:7.00E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 682214
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AIM14(YGL160W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ALG9(YNL219C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:AMN1(YBR158W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ATO3(YDR384C)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BRE2(YLR015W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BUD7(YOR299W)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:COX8(YLR395C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRG1(YHR209W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DAL7(YIR031C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DBP1(YPL119C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:4.32|P-value:7.71E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DCS1(YLR270W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:EFG1(YGR271C-A)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FYV7(YLR068W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLN3(YER040W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GPA2(YER020W)|FD-Score:5.32|P-value:5.23E-8||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:HEF3(YNL014W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HES1(YOR237W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:INM1(YHR046C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:INP51(YIL002C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IPT1(YDR072C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KGD1(YIL125W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LDB7(YBL006C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MDS3(YGL197W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MID1(YNL291C)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:MKS1(YNL076W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:NFU1(YKL040C)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:NOP6(YDL213C)|FD-Score:-5.17|P-value:1.16E-7||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:ORM2(YLR350W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDB1(YBR221C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PET112(YBL080C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PNG1(YPL096W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PNS1(YOR161C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POA1(YBR022W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:POX1(YGL205W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PRK1(YIL095W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRM1(YNL279W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUS9(YDL036C)|FD-Score:-4.81|P-value:7.38E-7||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:RAM1(YDL090C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:REH1(YLR387C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RKM3(YBR030W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RTG1(YOL067C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SAF1(YBR280C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAS4(YDR181C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SFL1(YOR140W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SHM2(YLR058C)|FD-Score:-4.6|P-value:2.11E-6||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SPO12(YHR152W)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SSA3(YBL075C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUL2(YLR092W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR4(YLR372W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THP1(YOL072W)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIP1(YBR067C)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRP1(YDR007W)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.24|P-value:8.05E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.31|P-value:5.39E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.78|P-value:8.59E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:5.23|P-value:8.54E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS52(YDR484W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBR196C-A(YBR196C-A_p)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL050C(YDL050C_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL144C(YDL144C_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:6.48|P-value:4.50E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR015C(YDR015C_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YGL117W(YGL117W_p)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative protein of unknown function Gene:YHM2(YMR241W)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YJL120W(YJL120W_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YMR074C(YMR074C)|FD-Score:3.72|P-value:9.81E-5||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL144C(YNL144C_p)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOR022C(YOR022C_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR378W(YOR378W_p)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:4.77|P-value:9.11E-7||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YRB30(YGL164C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YSP3(YOR003W)|FD-Score:-5.39|P-value:3.43E-8||SGD DESC:Putative precursor to the subtilisin-like protease III Gene:ADH4(YGL256W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AIM14(YGL160W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ALG9(YNL219C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:AMN1(YBR158W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ATO3(YDR384C)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:BRE2(YLR015W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BUD7(YOR299W)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:COX8(YLR395C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CRG1(YHR209W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DAL7(YIR031C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DBP1(YPL119C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DBP7(YKR024C)|FD-Score:4.32|P-value:7.71E-6||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DCS1(YLR270W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:EFG1(YGR271C-A)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:FYV7(YLR068W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:4.38|P-value:5.92E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GLN3(YER040W)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GPA2(YER020W)|FD-Score:5.32|P-value:5.23E-8||SGD DESC:Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:HEF3(YNL014W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HES1(YOR237W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); HES1 has a paralog, KES1, that arose from the whole genome duplication Gene:INM1(YHR046C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:INP51(YIL002C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IPT1(YDR072C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:KGD1(YIL125W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:LDB7(YBL006C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MDS3(YGL197W)|FD-Score:-3.38|P-value:3.66E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MID1(YNL291C)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:MKS1(YNL076W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling Gene:NFU1(YKL040C)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:NOP6(YDL213C)|FD-Score:-5.17|P-value:1.16E-7||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:ORM2(YLR350W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDB1(YBR221C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PET112(YBL080C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PNG1(YPL096W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PNS1(YOR161C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:POA1(YBR022W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:POX1(YGL205W)|FD-Score:-3.32|P-value:4.50E-4||SGD DESC:Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix Gene:PRK1(YIL095W)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PRM1(YNL279W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUS9(YDL036C)|FD-Score:-4.81|P-value:7.38E-7||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:RAM1(YDL090C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:REH1(YLR387C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RKM3(YBR030W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RTG1(YOL067C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:SAF1(YBR280C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SAS4(YDR181C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p Gene:SFL1(YOR140W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SHM2(YLR058C)|FD-Score:-4.6|P-value:2.11E-6||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SPO12(YHR152W)|FD-Score:5.02|P-value:2.57E-7||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SSA3(YBL075C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUL2(YLR092W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:SUR4(YLR372W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THP1(YOL072W)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIP1(YBR067C)|FD-Score:3.87|P-value:5.51E-5||SGD DESC:Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins Gene:TRP1(YDR007W)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.24|P-value:8.05E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.31|P-value:5.39E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.78|P-value:8.59E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:5.23|P-value:8.54E-8||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VPS52(YDR484W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBR196C-A(YBR196C-A_p)|FD-Score:5.46|P-value:2.36E-8||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL050C(YDL050C_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL144C(YDL144C_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:6.48|P-value:4.50E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR015C(YDR015C_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A Gene:YGL117W(YGL117W_p)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative protein of unknown function Gene:YHM2(YMR241W)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YJL120W(YJL120W_d)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YMR074C(YMR074C)|FD-Score:3.72|P-value:9.81E-5||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL144C(YNL144C_p)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOR022C(YOR022C_p)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR378W(YOR378W_p)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Putative paralog of ATR1, but not required for boron tolerance; member of the DHA2 family of drug:H+ antiporters; YOR378W is not an essential gene Gene:YPL257W(YPL257W_p)|FD-Score:4.77|P-value:9.11E-7||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YRB30(YGL164C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:YSP3(YOR003W)|FD-Score:-5.39|P-value:3.43E-8||SGD DESC:Putative precursor to the subtilisin-like protease III

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL004W5.181.09E-70.61AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
YKL078W4.943.86E-70.61DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YBL023C4.562.53E-60.61MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YDL148C3.953.91E-50.22NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YDL220C3.739.51E-50.16CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
YLL031C3.571.78E-40.01GPI13ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
YNL207W3.561.84E-40.37RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YLR076C_d3.197.00E-40.09YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YLR088W3.119.48E-40.05GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YKL154W3.060.001100.08SRP102Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
YMR236W2.980.001460.03TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YHR058C2.950.001610.01MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YKL036C_d2.940.001650.03YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YGL001C2.910.001830.07ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YDL209C2.840.002270.01CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d6.484.50E-11YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL026C5.658.12E-9TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YKR026C5.551.39E-8GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YBR196C-A_p5.462.36E-8YBR196C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YER020W5.325.23E-8GPA2Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YKL211C5.315.39E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YER090W5.248.05E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YBR058C-A5.238.54E-8TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR152W5.022.57E-7SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
YOR299W4.808.00E-7BUD7Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
YDR354W4.788.59E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YPL257W_p4.779.11E-7YPL257W_pPutative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
YBL075C4.592.26E-6SSA3ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
YEL009C4.473.99E-6GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YFR009W4.385.92E-6GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA

GO enrichment analysis for SGTC_668
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3083.62E-130SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0701754
0.2925.51E-116SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0405405
0.2901.65E-114SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0405405
0.2886.48E-113SGTC_18825615643 20.0 μMMiscellaneous22530750.126582TRP & mitochondrial translation
0.2705.08E-99SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.241379TSC3-RPN4
0.2689.20E-98SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0266667
0.2662.50E-96SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0724638
0.2651.03E-95SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.116667TRP & mitochondrial translation
0.2621.37E-93SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.126761
0.2622.14E-93SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.1125
0.2623.78E-93SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0625
0.2604.31E-92SGTC_24785763493 32.3 μMMiscellaneous13655760.0704225
0.2591.93E-91SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0266667
0.2583.97E-90SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0222222
0.2567.33E-89SGTC_18815652484 16.0 μMMiscellaneous22551290.1TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6530203-001655.2 μM0.681818295302ChemDiv (Drug-like library)289.756743.44212
SGTC_9621254-030021.5 μM0.4333337340576ChemDiv (Drug-like library)406.058743.79114
SGTC_1300136-0219337.66 μM0.384615234244ChemDiv (Drug-like library)296.148643.77812
SGTC_2888905854712.99 μM0.37516476514Chembridge (Drug-like library)279.333084.11812
SGTC_22436634756200 μM0.372549885676Chembridge (Fragment library)234.079262.01512
SGTC_74000s-0487160.28 μM0.37037677548ChemDiv (Drug-like library)277.702982.87223
SGTC_15120259-054412.2 μM0.35714318476ChemDiv (Drug-like library)293.1484.43412
SGTC_3053909590349.47 μM0.3517174018Chembridge (Drug-like library)317.766843.39313Golgi
SGTC_2929901334824.6 μM0.3392862995952Chembridge (Drug-like library)310.175223.99912
SGTC_12001643-019318.4 μM0.3382355403974ChemDiv (Drug-like library)347.753063.56315