0560-0003

(Z)-2-amino-3-[[4-(diethylamino)phenyl]methylideneamino]but-2-enedinitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_671
Screen concentration 407.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3238678
SMILES CCN(CC)C1=CC=C(C=C1)C=NC(=C(C#N)N)C#N
Standardized SMILES CCN(CC)c1ccc(C=NC(=C(N)C#N)C#N)cc1
Molecular weight 267.329
ALogP 1.5
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.57
% growth inhibition (Hom. pool) 6.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3238678
Download HIP data (tab-delimited text)  (excel)
Gene:ARP4(YJL081C)|FD-Score:4.68|P-value:1.43E-6|Clearance:1.4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:-4.1|P-value:2.03E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ESA1(YOR244W)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.3||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:LCB1(YMR296C)|FD-Score:10.8|P-value:2.27E-27|Clearance:4.99||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LCB2(YDR062W)|FD-Score:5.79|P-value:3.57E-9|Clearance:0.73||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MED8(YBR193C)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NDC1(YML031W)|FD-Score:5.06|P-value:2.13E-7|Clearance:0.38||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:RPB5(YBR154C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.13||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:UTP6(YDR449C)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.01||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-3.42|P-value:3.10E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ARP4(YJL081C)|FD-Score:4.68|P-value:1.43E-6|Clearance:1.4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP9(YMR033W)|FD-Score:-4.1|P-value:2.03E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ESA1(YOR244W)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.3||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:LCB1(YMR296C)|FD-Score:10.8|P-value:2.27E-27|Clearance:4.99||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LCB2(YDR062W)|FD-Score:5.79|P-value:3.57E-9|Clearance:0.73||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MED8(YBR193C)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NDC1(YML031W)|FD-Score:5.06|P-value:2.13E-7|Clearance:0.38||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:RPB5(YBR154C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.13||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:UTP6(YDR449C)|FD-Score:3.28|P-value:5.19E-4|Clearance:0.01||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-3.42|P-value:3.10E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3238678
Download HOP data (tab-delimited text)  (excel)
Gene:AEP3(YPL005W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM21(YIR003W)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ATH1(YPR026W)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:CCP1(YKR066C)|FD-Score:4.91|P-value:4.57E-7||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CRD1(YDL142C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:ECM23(YPL021W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:ERG2(YMR202W)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:EXG2(YDR261C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:GDH2(YDL215C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GUF1(YLR289W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:ISR1(YPR106W)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KRE2(YDR483W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LOS1(YKL205W)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MAK10(YEL053C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MLP1(YKR095W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MRC1(YCL061C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRP1(YDR347W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MSN2(YMR037C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC6(YHR151C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MVB12(YGR206W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:PKH2(YOL100W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRO2(YOR323C)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC1(YDL006W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTK2(YJR059W)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUB1(YNL016W)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RAD28(YDR030C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RFU1(YLR073C)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RSB1(YOR049C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RTC6(YPL183W-A)|FD-Score:4.27|P-value:9.77E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAP30(YMR263W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SCS7(YMR272C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SGA1(YIL099W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SHE4(YOR035C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHU2(YDR078C)|FD-Score:-4.28|P-value:9.33E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNT309(YPR101W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPS22(YCL048W)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall Gene:STF1(YDL130W-A)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWS2(YNL081C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:SYS1(YJL004C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA3(YHR009C_p)|FD-Score:5.05|P-value:2.19E-7||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TPP1(YMR156C)|FD-Score:6.05|P-value:7.28E-10||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRS85(YDR108W)|FD-Score:-4.56|P-value:2.62E-6||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSC3(YBR058C-A)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA1(YKL216W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VAM3(YOR106W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:YAL018C(YAL018C_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function Gene:YGR069W(YGR069W_d)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL060W(YIL060W_p)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKR073C(YKR073C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR039W(YPR039W_d)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YTA7(YGR270W)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:AEP3(YPL005W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:AIM21(YIR003W)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ATH1(YPR026W)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:CCP1(YKR066C)|FD-Score:4.91|P-value:4.57E-7||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CRD1(YDL142C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:ECM23(YPL021W)|FD-Score:3.24|P-value:6.08E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:ERG2(YMR202W)|FD-Score:5.6|P-value:1.05E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:EXG2(YDR261C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:GDH2(YDL215C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels Gene:GUF1(YLR289W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:ISR1(YPR106W)|FD-Score:3.81|P-value:7.06E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KRE2(YDR483W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LOS1(YKL205W)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MAK10(YEL053C)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible Gene:MLP1(YKR095W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MRC1(YCL061C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRP1(YDR347W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MSN2(YMR037C)|FD-Score:-3.52|P-value:2.17E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC6(YHR151C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MVB12(YGR206W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:PKH2(YOL100W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRO2(YOR323C)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC1(YDL006W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTK2(YJR059W)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUB1(YNL016W)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RAD28(YDR030C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RFU1(YLR073C)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RSB1(YOR049C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RTC6(YPL183W-A)|FD-Score:4.27|P-value:9.77E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAP30(YMR263W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SCS7(YMR272C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SGA1(YIL099W)|FD-Score:-3.57|P-value:1.81E-4||SGD DESC:Intracellular sporulation-specific glucoamylase involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation Gene:SHE4(YOR035C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHU2(YDR078C)|FD-Score:-4.28|P-value:9.33E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SNT309(YPR101W)|FD-Score:3.35|P-value:3.97E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SPS22(YCL048W)|FD-Score:-3.95|P-value:3.87E-5||SGD DESC:Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall Gene:STF1(YDL130W-A)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWS2(YNL081C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:SYS1(YJL004C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA3(YHR009C_p)|FD-Score:5.05|P-value:2.19E-7||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TPP1(YMR156C)|FD-Score:6.05|P-value:7.28E-10||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRS85(YDR108W)|FD-Score:-4.56|P-value:2.62E-6||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSC3(YBR058C-A)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URA1(YKL216W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VAM3(YOR106W)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:YAL018C(YAL018C_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Putative protein of unknown function Gene:YGR069W(YGR069W_d)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL060W(YIL060W_p)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKR073C(YKR073C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR039W(YPR039W_d)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YTA7(YGR270W)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR296C10.802.27E-274.99LCB1Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YDR062W5.793.57E-90.73LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YML031W5.062.13E-70.38NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YJL081C4.681.43E-61.40ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YDR449C3.285.19E-40.01UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YBR154C3.275.33E-40.13RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YOR244W3.148.31E-40.30ESA1Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy
YJL174W2.840.002220.01KRE9Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
YOR207C2.830.002330.09RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YBL077W_d2.740.003104.59E-5YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YOR282W_d2.740.003100.01YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YDR064W2.730.003200.10RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YGL001C2.630.004310.07ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YMR028W2.550.005350.09TAP42Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
YHR036W2.460.006880.05BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR156C6.057.28E-10TPP1DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase
YMR202W5.601.05E-8ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YOR323C5.121.54E-7PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YHR009C_p5.052.19E-7TDA3_pPutative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
YKR066C4.914.57E-7CCP1Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress
YIR003W4.493.59E-6AIM21Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton
YPL183W-A4.279.77E-6RTC6Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
YDL130W-A4.231.18E-5STF1Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress
YNL081C4.151.67E-5SWS2Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency
YDR030C4.042.62E-5RAD28Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair
YGR206W3.865.65E-5MVB12ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
YNL016W3.846.11E-5PUB1Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
YPR106W3.817.06E-5ISR1Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C
YLR073C3.797.45E-5RFU1Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes
YLR289W3.711.02E-4GUF1Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor

GO enrichment analysis for SGTC_671
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1411.54E-27SGTC_1876myriocin 605.0 nMMiscellaneous3011190.0821918
0.1248.84E-22SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.118644
0.1202.24E-20SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0625ERG2
0.1102.49E-17SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.0655738tubulin folding & SWR complex
0.1086.95E-17SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.133333TSC3-RPN4
0.1071.37E-16SGTC_1596piperlongumine 63.0 μMTimTec (Pure natural product library)6378580.084507
0.1072.20E-16SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0983607TSC3-RPN4
0.1032.01E-15SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0655738TSC3-RPN4
0.1032.39E-15SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0327869
0.1032.40E-15SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0632911TSC3-RPN4
0.1022.95E-15SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0333333ERG2
0.1018.73E-15SGTC_6990375-0592 111.0 μMChemDiv (Drug-like library)7625780.0769231iron homeostasis
0.1001.37E-14SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0547945ERG2
0.0961.09E-13SGTC_2699st077704 54.6 μMTimTec (Natural product derivative library)6458520.0714286endomembrane recycling
0.0961.47E-13SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0576923ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7521896-0090130 μM0.258621772567ChemDiv (Drug-like library)294.147182.04913TSC3-RPN4
SGTC_503tyrphostin 8294 μM0.23404392970ICCB bioactive library170.167441.77313TSC3-RPN4
SGTC_1651st01192886.1 μM0.224138720198TimTec (Natural product derivative library)232.258421.77414RSC complex & mRNA processing
SGTC_1652st01193278 μM0.2222221551076TimTec (Natural product derivative library)245.230742.10905RSC complex & mRNA processing
SGTC_7334069-000756.1 μM0.2131155717425ChemDiv (Drug-like library)309.193764.32814ERAD & cell cycle
SGTC_12490481-001510.2 μM0.2105264227577ChemDiv (Drug-like library)325.788844.18103
SGTC_774k060-0086136 μM0.2096775911235ChemDiv (Drug-like library)280.324443.67815
SGTC_462ag-879158 μM0.2068976809654ICCB bioactive library316.460963.88224
SGTC_6491123-0311367 μM0.20339693629ChemDiv (Drug-like library)241.28513.51613endomembrane recycling
SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione62.6 μM0.203125756377TimTec (Natural product derivative library)319.739662.86205TSC3-RPN4