0498-0004

4-[2-(4-amino-3,5-dimethylphenyl)propan-2-yl]-2,6-dimethylaniline

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_674
Screen concentration 451.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4231473
SMILES CC1=CC(=CC(=C1N)C)C(C)(C)C2=CC(=C(C(=C2)C)N)C
Standardized SMILES Cc1cc(cc(C)c1N)C(C)(C)c2cc(C)c(N)c(C)c2
Molecular weight 282.4231
ALogP 4.71
H-bond donor count 2
H-bond acceptor count 2
Response signature heme requiring

Pool Growth Kinetics
% growth inhibition (Het. pool) 41.96
% growth inhibition (Hom. pool) 6.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4231473
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.02||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ARP7(YPR034W)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:7.53|P-value:2.49E-14|Clearance:1.47||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:-4.59|P-value:2.25E-6|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:-3.41|P-value:3.31E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC5(YMR001C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.05||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CDC6(YJL194W)|FD-Score:4.74|P-value:1.06E-6|Clearance:0.06||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COP1(YDL145C)|FD-Score:5.11|P-value:1.65E-7|Clearance:0.36||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DBP6(YNR038W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.1||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:DFR1(YOR236W)|FD-Score:-4.46|P-value:4.19E-6|Clearance:0||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:DML1(YMR211W)|FD-Score:7.63|P-value:1.14E-14|Clearance:1.47||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DPM1(YPR183W)|FD-Score:3.36|P-value:3.83E-4|Clearance:0.15||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ERG27(YLR100W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.03||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:FAS1(YKL182W)|FD-Score:4.23|P-value:1.18E-5|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GPI2(YPL076W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.02||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HEM12(YDR047W)|FD-Score:8.55|P-value:5.96E-18|Clearance:1.47||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:4.48|P-value:3.73E-6|Clearance:0.25||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:ILV5(YLR355C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.03||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:-5.01|P-value:2.79E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:ISD11(YER048W-A)|FD-Score:4.09|P-value:2.19E-5|Clearance:0.01||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM2(YBL026W)|FD-Score:-3.83|P-value:6.28E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MNP1(YGL068W)|FD-Score:3.78|P-value:7.69E-5|Clearance:0.01||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:MTR10(YOR160W)|FD-Score:4.05|P-value:2.54E-5|Clearance:0.09||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NIP1(YMR309C)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NUS1(YDL193W)|FD-Score:-4.57|P-value:2.44E-6|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE4(YFR050C)|FD-Score:-3.61|P-value:1.51E-4|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RAT1(YOR048C)|FD-Score:-4.48|P-value:3.68E-6|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:REB1(YBR049C)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.12||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RIM2(YBR192W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.07||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:-4.28|P-value:9.38E-6|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:-5.56|P-value:1.34E-8|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB7(YDR404C)|FD-Score:-5.21|P-value:9.38E-8|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:-4.3|P-value:8.55E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-4.19|P-value:1.40E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPC11(YDR045C)|FD-Score:3.77|P-value:8.08E-5|Clearance:0.04||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL10(YLR075W)|FD-Score:-3.11|P-value:9.39E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL32(YBL092W)|FD-Score:-4.75|P-value:1.03E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN2(YIL075C)|FD-Score:5.26|P-value:7.04E-8|Clearance:0.16||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:-4.7|P-value:1.28E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC9(YML127W)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC53(YFL045C)|FD-Score:-3.83|P-value:6.34E-5|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SPC98(YNL126W)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.01||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SUP45(YBR143C)|FD-Score:5.72|P-value:5.47E-9|Clearance:0.45||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TIF35(YDR429C)|FD-Score:-5.04|P-value:2.30E-7|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM12(YBR091C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TIM17(YJL143W)|FD-Score:-7.05|P-value:8.97E-13|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM22(YDL217C)|FD-Score:4.06|P-value:2.49E-5|Clearance:0||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TIM23(YNR017W)|FD-Score:-3.49|P-value:2.40E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TOM40(YMR203W)|FD-Score:7.19|P-value:3.32E-13|Clearance:1.47||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP22(YGR090W)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.12||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:WRS1(YOL097C)|FD-Score:-3.24|P-value:5.94E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGL074C(YGL074C_d)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YJL086C(YJL086C_d)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR317W(YLR317W_d)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.03||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YNL114C(YNL114C_d)|FD-Score:3.74|P-value:9.34E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YOR203W(YOR203W_d)|FD-Score:3.72|P-value:9.90E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPL142C(YPL142C_d)|FD-Score:-6.31|P-value:1.35E-10|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YTH1(YPR107C)|FD-Score:7.94|P-value:1.01E-15|Clearance:1.47||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ABF1(YKL112W)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.02||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ARP7(YPR034W)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:7.53|P-value:2.49E-14|Clearance:1.47||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC20(YGL116W)|FD-Score:-4.59|P-value:2.25E-6|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:-3.41|P-value:3.31E-4|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CDC5(YMR001C)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.05||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CDC6(YJL194W)|FD-Score:4.74|P-value:1.06E-6|Clearance:0.06||SGD DESC:Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COP1(YDL145C)|FD-Score:5.11|P-value:1.65E-7|Clearance:0.36||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DBP6(YNR038W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.1||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:DFR1(YOR236W)|FD-Score:-4.46|P-value:4.19E-6|Clearance:0||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:DML1(YMR211W)|FD-Score:7.63|P-value:1.14E-14|Clearance:1.47||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:DPM1(YPR183W)|FD-Score:3.36|P-value:3.83E-4|Clearance:0.15||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:ERG27(YLR100W)|FD-Score:3.53|P-value:2.05E-4|Clearance:0.03||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:FAS1(YKL182W)|FD-Score:4.23|P-value:1.18E-5|Clearance:0.03||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GPI2(YPL076W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.02||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HEM12(YDR047W)|FD-Score:8.55|P-value:5.96E-18|Clearance:1.47||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM3(YDL205C)|FD-Score:4.48|P-value:3.73E-6|Clearance:0.25||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:ILV5(YLR355C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.03||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:-5.01|P-value:2.79E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:ISD11(YER048W-A)|FD-Score:4.09|P-value:2.19E-5|Clearance:0.01||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LSM2(YBL026W)|FD-Score:-3.83|P-value:6.28E-5|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MNP1(YGL068W)|FD-Score:3.78|P-value:7.69E-5|Clearance:0.01||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:MTR10(YOR160W)|FD-Score:4.05|P-value:2.54E-5|Clearance:0.09||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NIP1(YMR309C)|FD-Score:-3.15|P-value:8.17E-4|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NUS1(YDL193W)|FD-Score:-4.57|P-value:2.44E-6|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PRE4(YFR050C)|FD-Score:-3.61|P-value:1.51E-4|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RAT1(YOR048C)|FD-Score:-4.48|P-value:3.68E-6|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:REB1(YBR049C)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.12||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RIM2(YBR192W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.07||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPA43(YOR340C)|FD-Score:-4.28|P-value:9.38E-6|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:-5.56|P-value:1.34E-8|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB7(YDR404C)|FD-Score:-5.21|P-value:9.38E-8|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:-4.3|P-value:8.55E-6|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-4.19|P-value:1.40E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPC11(YDR045C)|FD-Score:3.77|P-value:8.08E-5|Clearance:0.04||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RPL10(YLR075W)|FD-Score:-3.11|P-value:9.39E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL32(YBL092W)|FD-Score:-4.75|P-value:1.03E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPN2(YIL075C)|FD-Score:5.26|P-value:7.04E-8|Clearance:0.16||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPS5(YJR123W)|FD-Score:-4.7|P-value:1.28E-6|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:RSC9(YML127W)|FD-Score:4.56|P-value:2.53E-6|Clearance:0.08||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC53(YFL045C)|FD-Score:-3.83|P-value:6.34E-5|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SPC98(YNL126W)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.01||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SUP45(YBR143C)|FD-Score:5.72|P-value:5.47E-9|Clearance:0.45||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TIF35(YDR429C)|FD-Score:-5.04|P-value:2.30E-7|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM12(YBR091C)|FD-Score:-3.48|P-value:2.46E-4|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TIM17(YJL143W)|FD-Score:-7.05|P-value:8.97E-13|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM22(YDL217C)|FD-Score:4.06|P-value:2.49E-5|Clearance:0||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TIM23(YNR017W)|FD-Score:-3.49|P-value:2.40E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TOM40(YMR203W)|FD-Score:7.19|P-value:3.32E-13|Clearance:1.47||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP22(YGR090W)|FD-Score:3.91|P-value:4.69E-5|Clearance:0.12||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:WRS1(YOL097C)|FD-Score:-3.24|P-value:5.94E-4|Clearance:0||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGL074C(YGL074C_d)|FD-Score:3.96|P-value:3.70E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YJL086C(YJL086C_d)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR317W(YLR317W_d)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.03||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YNL114C(YNL114C_d)|FD-Score:3.74|P-value:9.34E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YOR203W(YOR203W_d)|FD-Score:3.72|P-value:9.90E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPL142C(YPL142C_d)|FD-Score:-6.31|P-value:1.35E-10|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:YTH1(YPR107C)|FD-Score:7.94|P-value:1.01E-15|Clearance:1.47||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4231473
Download HOP data (tab-delimited text)  (excel)
Gene:AHT1(YHR093W_d)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ALB1(YJL122W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ALG5(YPL227C)|FD-Score:4.27|P-value:9.61E-6||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APP1(YNL094W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATG11(YPR049C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:AUA1(YFL010W-A)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:AVO2(YMR068W)|FD-Score:4.89|P-value:4.95E-7||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:CCP1(YKR066C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CUE5(YOR042W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:EAF7(YNL136W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:FAR7(YFR008W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GDB1(YPR184W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HFM1(YGL251C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HIT1(YJR055W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HPA2(YPR193C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:ICT1(YLR099C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:7.59|P-value:1.59E-14||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC2(YDR112W_d)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LRG1(YDL240W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LSC1(YOR142W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:LYP1(YNL268W)|FD-Score:7.15|P-value:4.27E-13||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDY2(YOL111C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEH1(YKR007W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MFB1(YDR219C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MON2(YNL297C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NIF3(YGL221C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NIP100(YPL174C)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NIT1(YIL164C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NKP1(YDR383C)|FD-Score:-5.09|P-value:1.77E-7||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NKP2(YLR315W)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:PEX6(YNL329C)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PFK1(YGR240C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POF1(YCL047C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:RHO4(YKR055W)|FD-Score:5.39|P-value:3.44E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:ROM2(YLR371W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL12B(YDR418W)|FD-Score:-4.98|P-value:3.21E-7||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:RTT101(YJL047C)|FD-Score:4.86|P-value:5.86E-7||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SCO2(YBR024W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLO1(YER180C-A)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SNT309(YPR101W)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SRO77(YBL106C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STM1(YLR150W)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SUR4(YLR372W)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWS2(YNL081C)|FD-Score:-4.78|P-value:8.55E-7||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TED1(YIL039W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TMA64(YDR117C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPO4(YOR273C)|FD-Score:5.82|P-value:2.93E-9||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRS85(YDR108W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:URM1(YIL008W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAM7(YGL212W)|FD-Score:-4.97|P-value:3.39E-7||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPH1(YOR270C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS25(YJR102C)|FD-Score:5.88|P-value:2.01E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YBR238C(YBR238C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL094C(YDL094C_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR271C(YDR271C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR391C(YDR391C_p)|FD-Score:4.9|P-value:4.79E-7||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR433W(YDR433W_d)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL008C(YHL008C_p)|FD-Score:4.42|P-value:5.03E-6||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR210C(YHR210C_p)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIR042C(YIR042C_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YKL222C(YKL222C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR030W(YLR030W_p)|FD-Score:-4.3|P-value:8.50E-6||SGD DESC:Putative protein of unknown function Gene:YMR166C(YMR166C_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR244W(YMR244W_p)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function Gene:YNL134C(YNL134C_p)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YOR053W(YOR053W_d)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR072W(YOR072W_d)|FD-Score:5.09|P-value:1.81E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR097W(YPR097W)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR170C(YPR170C_d)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YSP1(YHR155W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:AHT1(YHR093W_d)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region Gene:ALB1(YJL122W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p Gene:ALG5(YPL227C)|FD-Score:4.27|P-value:9.61E-6||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APP1(YNL094W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:ATG11(YPR049C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:AUA1(YFL010W-A)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:AVO2(YMR068W)|FD-Score:4.89|P-value:4.95E-7||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:CCP1(YKR066C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:CUE5(YOR042W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:EAF7(YNL136W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:FAR7(YFR008W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GDB1(YPR184W)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:HFM1(YGL251C)|FD-Score:4.35|P-value:6.85E-6||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HIT1(YJR055W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HPA2(YPR193C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:ICT1(YLR099C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:7.59|P-value:1.59E-14||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC2(YDR112W_d)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:LRG1(YDL240W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LSC1(YOR142W)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:LYP1(YNL268W)|FD-Score:7.15|P-value:4.27E-13||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDY2(YOL111C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEH1(YKR007W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MFB1(YDR219C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MON2(YNL297C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NIF3(YGL221C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NIP100(YPL174C)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:NIT1(YIL164C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NKP1(YDR383C)|FD-Score:-5.09|P-value:1.77E-7||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4 Gene:NKP2(YLR315W)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:PEX6(YNL329C)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol Gene:PFK1(YGR240C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POF1(YCL047C)|FD-Score:-3.1|P-value:9.64E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:RHO4(YKR055W)|FD-Score:5.39|P-value:3.44E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:ROM2(YLR371W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL12B(YDR418W)|FD-Score:-4.98|P-value:3.21E-7||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RRM3(YHR031C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:RTT101(YJL047C)|FD-Score:4.86|P-value:5.86E-7||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SCO2(YBR024W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SCP1(YOR367W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLO1(YER180C-A)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO Gene:SNT309(YPR101W)|FD-Score:4.34|P-value:7.20E-6||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SRO77(YBL106C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STM1(YLR150W)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SUR4(YLR372W)|FD-Score:3.21|P-value:6.52E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWS2(YNL081C)|FD-Score:-4.78|P-value:8.55E-7||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TED1(YIL039W)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TMA64(YDR117C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TPO4(YOR273C)|FD-Score:5.82|P-value:2.93E-9||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRS85(YDR108W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:URM1(YIL008W)|FD-Score:-3.11|P-value:9.33E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAM7(YGL212W)|FD-Score:-4.97|P-value:3.39E-7||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPH1(YOR270C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS25(YJR102C)|FD-Score:5.88|P-value:2.01E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YBR238C(YBR238C)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL094C(YDL094C_d)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR271C(YDR271C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR391C(YDR391C_p)|FD-Score:4.9|P-value:4.79E-7||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR433W(YDR433W_d)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHL008C(YHL008C_p)|FD-Score:4.42|P-value:5.03E-6||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR210C(YHR210C_p)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIR042C(YIR042C_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YKL222C(YKL222C)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR030W(YLR030W_p)|FD-Score:-4.3|P-value:8.50E-6||SGD DESC:Putative protein of unknown function Gene:YMR166C(YMR166C_p)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR244W(YMR244W_p)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function Gene:YNL134C(YNL134C_p)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:YOR053W(YOR053W_d)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR072W(YOR072W_d)|FD-Score:5.09|P-value:1.81E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR097W(YPR097W)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR170C(YPR170C_d)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YSP1(YHR155W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W8.555.96E-181.47HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YPR107C7.941.01E-151.47YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YMR211W7.631.14E-141.47DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YHR036W7.532.49E-141.47BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YMR203W7.193.32E-131.47TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YBR143C5.725.47E-90.45SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YIL075C5.267.04E-80.16RPN2Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
YDL145C5.111.65E-70.36COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YJL194W4.741.06E-60.06CDC6Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YBR049C4.681.42E-60.12REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YML127W4.562.53E-60.08RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YDL205C4.483.73E-60.25HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YKL182W4.231.18E-50.03FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YNL126W4.201.33E-50.01SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YLR355C4.191.41E-50.03ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR073C7.591.59E-14IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YNL268W7.154.27E-13LYP1Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
YJR102C5.882.01E-9VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YOR273C5.822.93E-9TPO4Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
YOR053W_d5.492.03E-8YOR053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
YKR055W5.393.44E-8RHO4Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity
YOR072W_d5.091.81E-7YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YDR391C_p4.904.79E-7YDR391C_pPutative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YMR068W4.894.95E-7AVO2Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
YJL047C4.865.86E-7RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YBR238C4.641.71E-6YBR238CMitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
YHR210C_p4.602.09E-6YHR210C_pPutative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
YNL329C4.454.31E-6PEX6AAA-peroxin that heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol
YHL008C_p4.425.03E-6YHL008C_pPutative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YPR184W4.415.17E-6GDB1Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_674
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2174.99E-64SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.037037heme biosynthesis & mitochondrial translocase
0.1891.19E-48SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0517241heme biosynthesis & mitochondrial translocase
0.1845.54E-46SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0952381heme requiring
0.1716.10E-40SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.0625
0.1633.07E-36SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.113208heme biosynthesis & mitochondrial translocase
0.1531.81E-32SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.097561superoxide
0.1512.48E-31SGTC_1301naphtanilide LB 4.8 μMChemDiv (Drug-like library)672380.0535714heme requiring
0.1461.28E-29SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0392157heme biosynthesis & mitochondrial translocase
0.1447.64E-29SGTC_9661300-0360 30.2 μMChemDiv (Drug-like library)705880.0454545heme requiring
0.1441.12E-28SGTC_208k072-0098 12.4 μMChemDiv (Drug-like library)59489010.0638298heme biosynthesis & mitochondrial translocase
0.1431.70E-28SGTC_29809014342 25.3 μMChembridge (Drug-like library)29965990.0588235
0.1411.05E-27SGTC_10441222-0021 1.8 μMChemDiv (Drug-like library)43067030.0555556heme biosynthesis & mitochondrial translocase
0.1374.02E-26SGTC_1785bathophenanthroline 24.0 μMMiscellaneous728120.0526316heme requiring
0.1321.64E-24SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.0392157heme biosynthesis & mitochondrial translocase
0.1314.00E-24SGTC_1831st055328 12.0 μMTimTec (Natural product derivative library)11030680.04heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)150 μM0.2222222374ICCB bioactive library222.323284.14622Golgi
SGTC_2176580977433.97 μM0.2105262870539Chembridge (Fragment library)240.752323.38123mitochondrial processes
SGTC_2131toluylene red5.29 μM0.20512811105Chembridge (Fragment library)288.775283.3432460S ribosome export
SGTC_2166593306131.5 μM0.2051282877375Chembridge (Fragment library)240.752323.38123tubulin folding & SWR complex
SGTC_20315180455200 μM0.229949Chembridge (Fragment library)177.031241.66522
SGTC_6140462-0418107 μM0.1951225188303ChemDiv (Drug-like library)256.303043.17625
SGTC_22987976326193.81 μM0.1951222235818Chembridge (Fragment library)203.237121.95623
SGTC_7690302-023176 μM0.194444571869ChemDiv (Drug-like library)225.2894.42513
SGTC_20084012253172 μM0.194444603940Chembridge (Fragment library)185.225142.05513
SGTC_23699071472200 μM0.19444417296037Chembridge (Fragment library)219.774622.90722