0527-0193

4-[(3-nitroanilino)diazenyl]benzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_688
Screen concentration 473.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4007154
SMILES C1=CC(=CC(=C1)[N+](=O)[O-])NN=NC2=CC=C(C=C2)C(=O)O
Standardized SMILES OC(=O)c1ccc(NN=Nc2cccc(c2)[N+](=O)[O-])cc1
Molecular weight 286.2429
ALogP 3.11
H-bond donor count 2
H-bond acceptor count 7
Response signature fatty acid desaturase (OLE1)

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.6
% growth inhibition (Hom. pool) 5.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4007154
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.21||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:BRN1(YBL097W)|FD-Score:4.71|P-value:1.21E-6|Clearance:0.56||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:HSH49(YOR319W)|FD-Score:4.54|P-value:2.87E-6|Clearance:0.56||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:OLE1(YGL055W)|FD-Score:4.46|P-value:4.08E-6|Clearance:0.56||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RHO3(YIL118W)|FD-Score:4.15|P-value:1.63E-5|Clearance:0.56||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPO31(YOR116C)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:-3.31|P-value:4.69E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TIM10(YHR005C-A)|FD-Score:3.39|P-value:3.54E-4|Clearance:0.3||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YLR101C(YLR101C_d)|FD-Score:-3.59|P-value:1.65E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W Gene:ATM1(YMR301C)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.21||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:BRN1(YBL097W)|FD-Score:4.71|P-value:1.21E-6|Clearance:0.56||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:HSH49(YOR319W)|FD-Score:4.54|P-value:2.87E-6|Clearance:0.56||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:OLE1(YGL055W)|FD-Score:4.46|P-value:4.08E-6|Clearance:0.56||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RHO3(YIL118W)|FD-Score:4.15|P-value:1.63E-5|Clearance:0.56||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p Gene:RPO31(YOR116C)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:-3.31|P-value:4.69E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TIM10(YHR005C-A)|FD-Score:3.39|P-value:3.54E-4|Clearance:0.3||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YLR101C(YLR101C_d)|FD-Score:-3.59|P-value:1.65E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4007154
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALF1(YNL148C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ATG18(YFR021W)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP25(YMR098C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BTS1(YPL069C)|FD-Score:6.06|P-value:6.72E-10||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR1(YPR124W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:EMC6(YLL014W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ERG2(YMR202W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERI1(YPL096C-A)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FET4(YMR319C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRK1(YPL141C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUS1(YCL027W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GCN5(YGR252W)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:9.05|P-value:7.00E-20||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIP1(YBR045C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GUT1(YHL032C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HTA2(YBL003C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:ICY1(YMR195W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:JLP1(YLL057C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:KAP104(YBR017C)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LYP1(YNL268W)|FD-Score:5.27|P-value:7.01E-8||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MAC1(YMR021C)|FD-Score:4.83|P-value:6.72E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDY2(YOL111C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MRPL40(YPL173W)|FD-Score:6.35|P-value:1.11E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NKP2(YLR315W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:PEP12(YOR036W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:RCY1(YJL204C)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RSM25(YIL093C)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RUP1(YOR138C)|FD-Score:-3.82|P-value:6.72E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SWS2(YNL081C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TIM8(YJR135W-A)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TPO4(YOR273C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:UTH1(YKR042W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:YAL018C(YAL018C_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function Gene:YDL180W(YDL180W_p)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR426C(YDR426C_d)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YER158C(YER158C_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YLR030W(YLR030W_p)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YNR063W(YNR063W_p)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL077C(YPL077C_p)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPR003C(YPR003C_p)|FD-Score:-5.36|P-value:4.12E-8||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:AFT1(YGL071W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALF1(YNL148C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ATG18(YFR021W)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATP25(YMR098C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BTS1(YPL069C)|FD-Score:6.06|P-value:6.72E-10||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CDC50(YCR094W)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR1(YPR124W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:EMC6(YLL014W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ERG2(YMR202W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERI1(YPL096C-A)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FET4(YMR319C)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRK1(YPL141C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUS1(YCL027W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Membrane protein localized to the shmoo tip, required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate Gene:GCN5(YGR252W)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCS1(YDL226C)|FD-Score:9.05|P-value:7.00E-20||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIP1(YBR045C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GUT1(YHL032C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HTA2(YBL003C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:ICY1(YMR195W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:JLP1(YLL057C)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:KAP104(YBR017C)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis Gene:LYP1(YNL268W)|FD-Score:5.27|P-value:7.01E-8||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MAC1(YMR021C)|FD-Score:4.83|P-value:6.72E-7||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDY2(YOL111C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MRPL40(YPL173W)|FD-Score:6.35|P-value:1.11E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NKP2(YLR315W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:PEP12(YOR036W)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:RCY1(YJL204C)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RSM25(YIL093C)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RUP1(YOR138C)|FD-Score:-3.82|P-value:6.72E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SWS2(YNL081C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TIM8(YJR135W-A)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TPO4(YOR273C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:UTH1(YKR042W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:YAL018C(YAL018C_p)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function Gene:YDL180W(YDL180W_p)|FD-Score:4.13|P-value:1.84E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR426C(YDR426C_d)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41 Gene:YER158C(YER158C_p)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YLR030W(YLR030W_p)|FD-Score:-3.62|P-value:1.50E-4||SGD DESC:Putative protein of unknown function Gene:YLR434C(YLR434C_d)|FD-Score:3.8|P-value:7.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YNR063W(YNR063W_p)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YPL077C(YPL077C_p)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPR003C(YPR003C_p)|FD-Score:-5.36|P-value:4.12E-8||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL097W4.711.21E-60.56BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YOR319W4.542.87E-60.56HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YGL055W4.464.08E-60.56OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YIL118W4.151.63E-50.56RHO3Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
YMR301C3.601.60E-40.21ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YHR005C-A3.393.54E-40.30TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YLR051C3.090.001010.01FCF2Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon
YOR340C3.080.001030.18RPA43RNA polymerase I subunit A43
YMR203W2.900.001890.07TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YLR175W2.830.002340.03CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YNL240C2.790.002600.16NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YLR340W2.630.004240.01RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YLR029C2.620.004360.01RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YMR131C2.620.004460.00RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YPL076W2.610.004510.02GPI2Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C9.057.00E-20GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YPL173W6.351.11E-10MRPL40Mitochondrial ribosomal protein of the large subunit
YPL069C6.066.72E-10BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YJL204C5.501.89E-8RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YNL268W5.277.01E-8LYP1Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
YMR021C4.836.72E-7MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YPR124W4.751.01E-6CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YCR094W4.681.45E-6CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YOR273C4.434.76E-6TPO4Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
YDL180W_p4.131.84E-5YDL180W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
YGR252W4.022.88E-5GCN5Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
YGL005C3.973.54E-5COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR315W3.963.72E-5NKP2Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
YPL096C-A3.924.34E-5ERI1Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
YFR021W3.856.02E-5ATG18Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood

GO enrichment analysis for SGTC_688
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2959.22E-119SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.310345fatty acid desaturase (OLE1)
0.2626.45E-93SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.64fatty acid desaturase (OLE1)
0.2544.18E-87SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0677966fatty acid desaturase (OLE1)
0.2543.26E-87SGTC_258haloperidol 50.8 μMMiscellaneous35590.12fatty acid desaturase (OLE1)
0.2273.96E-70SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.08fatty acid desaturase (OLE1)
0.2174.95E-64SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.0649351endomembrane recycling
0.2124.42E-61SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.0833333fatty acid desaturase (OLE1)
0.2111.80E-60SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.078125fatty acid desaturase (OLE1)
0.2093.53E-59SGTC_254nsc-64875 3.8 μMMiscellaneous56993NAergosterol depletion effects on membrane
0.2074.54E-58SGTC_2721ifenprodil 61.4 μMMiscellaneous36890.0675676plasma membrane duress
0.2037.67E-56SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.152778fatty acid desaturase (OLE1)
0.1971.38E-52SGTC_2725haloperidol 53.2 μMMiscellaneous35590.12plasma membrane duress
0.1961.98E-52SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.132353fatty acid desaturase (OLE1)
0.1964.95E-52SGTC_29939054257 71.4 μMChembridge (Drug-like library)64702170.132353fatty acid desaturase (OLE1)
0.1937.58E-51SGTC_577r071-0003 38.6 μMChemDiv (Drug-like library)45685170.101695fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8680527-0199213 μM0.642849450ChemDiv (Drug-like library)365.138963.85727fatty acid desaturase (OLE1)
SGTC_1150527-0192112.04 μM0.524233931ChemDiv (Drug-like library)276.678464.14315
SGTC_9262268-140159.4 μM0.406782931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_5671080-054268.4 μM0.3928575950415ChemDiv (Drug-like library)296.277483.3115
SGTC_166k072-0058166.42 μM0.379315846229ChemDiv (Drug-like library)308.255254.0227DNA intercalators
SGTC_155k072-020243.6 μM0.3728815857737ChemDiv (Drug-like library)332.352644.63825
SGTC_6260214-000933 μM0.3636363437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_11971601-0112267 μM0.3606567350313ChemDiv (Drug-like library)299.281422.31625
SGTC_1025k007-073170 μM0.3584916256118ChemDiv (Drug-like library)272.2743233.26425
SGTC_4260988-003714.6 μM0.3508775383008ChemDiv (Drug-like library)257.244741.51425ERAD & cell cycle