0508-1955

(E)-N-[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]-3-phenylprop-2-en-1-imine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_697
Screen concentration 564.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6243113
SMILES CC1=CC2=C(C=C1)N=C(S2)C3=CC=C(C=C3)N=CC=CC4=CC=CC=C4
Standardized SMILES Cc1ccc2nc(sc2c1)c3ccc(cc3)N=CC=Cc4ccccc4
Molecular weight 354.4674
ALogP 6.28
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.66
% growth inhibition (Hom. pool) 14.75


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6243113
Download HIP data (tab-delimited text)  (excel)
Gene:CLP1(YOR250C)|FD-Score:3.72|P-value:9.92E-5|Clearance:0.35||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GPI12(YMR281W)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.91||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:INO80(YGL150C)|FD-Score:-4.73|P-value:1.12E-6|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MGE1(YOR232W)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:POP8(YBL018C)|FD-Score:-3.18|P-value:7.36E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE8(YML092C)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.29||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPP1(YHR062C)|FD-Score:6.8|P-value:5.37E-12|Clearance:2.16||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC31(YDL195W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:CLP1(YOR250C)|FD-Score:3.72|P-value:9.92E-5|Clearance:0.35||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GPI12(YMR281W)|FD-Score:4.64|P-value:1.78E-6|Clearance:0.91||SGD DESC:ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp Gene:INO80(YGL150C)|FD-Score:-4.73|P-value:1.12E-6|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:MGE1(YOR232W)|FD-Score:-3.34|P-value:4.22E-4|Clearance:0||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:POP8(YBL018C)|FD-Score:-3.18|P-value:7.36E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE8(YML092C)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.29||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPP1(YHR062C)|FD-Score:6.8|P-value:5.37E-12|Clearance:2.16||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC31(YDL195W)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6243113
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:ADH5(YBR145W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AHC1(YOR023C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALD3(YMR169C)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ARG3(YJL088W)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL3(YPL051W)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG10(YLL042C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BFA1(YJR053W)|FD-Score:6.45|P-value:5.42E-11||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:BTS1(YPL069C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD31(YCR063W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CDC50(YCR094W)|FD-Score:4.79|P-value:8.42E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN1(YOR349W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COG6(YNL041C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8|P-value:6.04E-16||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSG2(YBR036C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM1(YCR086W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CUP9(YPL177C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAL82(YNL314W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:ELP3(YPL086C)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ELP4(YPL101W)|FD-Score:8.22|P-value:9.87E-17||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FLO11(YIR019C)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FSH1(YHR049W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GCN3(YKR026C)|FD-Score:4.88|P-value:5.34E-7||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCV3(YAL044C)|FD-Score:4.72|P-value:1.21E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GLG1(YKR058W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GRX1(YCL035C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GUP2(YPL189W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:GUT2(YIL155C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HHF1(YBR009C)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HMT1(YBR034C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:IBA57(YJR122W)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:ISU1(YPL135W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:IZH2(YOL002C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KIN2(YLR096W)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LIP5(YOR196C)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LRS4(YDR439W)|FD-Score:5.26|P-value:7.19E-8||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LTE1(YAL024C)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MEF1(YLR069C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MSH1(YHR120W)|FD-Score:5.71|P-value:5.67E-9||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSI1(YBR195C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NRG2(YBR066C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OST3(YOR085W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PEP7(YDR323C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET111(YMR257C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PHO88(YBR106W)|FD-Score:4.79|P-value:8.31E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POR1(YNL055C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PUS1(YPL212C)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PYK2(YOR347C)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAV1(YJR033C)|FD-Score:4.84|P-value:6.44E-7||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPL13B(YMR142C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTC5(YOR118W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:RTT106(YNL206C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SER1(YOR184W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SIP5(YMR140W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SNA2(YDR525W-A)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOD1(YJR104C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SWA2(YDR320C)|FD-Score:7.83|P-value:2.50E-15||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SYS1(YJL004C)|FD-Score:4.94|P-value:3.91E-7||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TOM6(YOR045W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRP2(YER090W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRS85(YDR108W)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VAM10(YOR068C)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VMA7(YGR020C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS35(YJL154C)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS61(YDR136C_d)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR197C(YBR197C_p)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YDL023C(YDL023C_d)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDR008C(YDR008C_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:14.7|P-value:1.86E-49||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEA6(YEL006W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGL072C(YGL072C_d)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR053C(YGR053C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHL008C(YHL008C_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIH1(YCR059C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL029C(YIL029C_p)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJR098C(YJR098C_p)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL058W(YLL058W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function Gene:YNL195C(YNL195C_p)|FD-Score:-3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL228W(YNL228W_d)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YOL114C(YOL114C_p)|FD-Score:5.42|P-value:2.92E-8||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YPS6(YIR039C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT6(YLR262C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRB30(YGL164C)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:AAH1(YNL141W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:ADH5(YBR145W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AHC1(YOR023C)|FD-Score:3.56|P-value:1.82E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALD3(YMR169C)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:ARG3(YJL088W)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARL3(YPL051W)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARO2(YGL148W)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG10(YLL042C)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BFA1(YJR053W)|FD-Score:6.45|P-value:5.42E-11||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:BTS1(YPL069C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD31(YCR063W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CDC50(YCR094W)|FD-Score:4.79|P-value:8.42E-7||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CIN1(YOR349W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COG6(YNL041C)|FD-Score:3.76|P-value:8.58E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8|P-value:6.04E-16||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSG2(YBR036C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM1(YCR086W)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CUP9(YPL177C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAL82(YNL314W)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:ELP3(YPL086C)|FD-Score:-4.09|P-value:2.19E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ELP4(YPL101W)|FD-Score:8.22|P-value:9.87E-17||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FLO11(YIR019C)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FSH1(YHR049W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GCN3(YKR026C)|FD-Score:4.88|P-value:5.34E-7||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCV3(YAL044C)|FD-Score:4.72|P-value:1.21E-6||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GLG1(YKR058W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication Gene:GRX1(YCL035C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GUP2(YPL189W)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog Gene:GUT2(YIL155C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HHF1(YBR009C)|FD-Score:-3.98|P-value:3.49E-5||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HMT1(YBR034C)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:IBA57(YJR122W)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:ISU1(YPL135W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:IZH2(YOL002C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:KIN2(YLR096W)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LIP5(YOR196C)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LRS4(YDR439W)|FD-Score:5.26|P-value:7.19E-8||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LTE1(YAL024C)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MEF1(YLR069C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MSH1(YHR120W)|FD-Score:5.71|P-value:5.67E-9||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSI1(YBR195C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NRG2(YBR066C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OST3(YOR085W)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PEP7(YDR323C)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET111(YMR257C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PHO88(YBR106W)|FD-Score:4.79|P-value:8.31E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:POR1(YNL055C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PUS1(YPL212C)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PYK2(YOR347C)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAV1(YJR033C)|FD-Score:4.84|P-value:6.44E-7||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPL13B(YMR142C)|FD-Score:3.77|P-value:8.05E-5||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL24B(YGR148C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.93|P-value:4.02E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RTC5(YOR118W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity Gene:RTT106(YNL206C)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SER1(YOR184W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SIP5(YMR140W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SNA2(YDR525W-A)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOD1(YJR104C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SWA2(YDR320C)|FD-Score:7.83|P-value:2.50E-15||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SYS1(YJL004C)|FD-Score:4.94|P-value:3.91E-7||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TOM6(YOR045W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRP2(YER090W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRS85(YDR108W)|FD-Score:3.89|P-value:4.98E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:VAM10(YOR068C)|FD-Score:3.73|P-value:9.53E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VMA7(YGR020C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS35(YJL154C)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS61(YDR136C_d)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR197C(YBR197C_p)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication Gene:YDL023C(YDL023C_d)|FD-Score:3.91|P-value:4.68E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDR008C(YDR008C_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR455C(YDR455C_d)|FD-Score:14.7|P-value:1.86E-49||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEA6(YEL006W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YGL072C(YGL072C_d)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR053C(YGR053C_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:5.01|P-value:2.76E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHL008C(YHL008C_p)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIH1(YCR059C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL029C(YIL029C_p)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJR098C(YJR098C_p)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL058W(YLL058W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YMR242W-A(YMR242W-A_p)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Putative protein of unknown function Gene:YNL195C(YNL195C_p)|FD-Score:-3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL228W(YNL228W_d)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YOL114C(YOL114C_p)|FD-Score:5.42|P-value:2.92E-8||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YPS6(YIR039C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:YPT6(YLR262C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRB30(YGL164C)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C6.805.37E-122.16RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR281W4.641.78E-60.92GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
YOR250C3.729.92E-50.35CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YML092C3.373.73E-40.29PRE8Alpha 2 subunit of the 20S proteasome
YCR054C3.080.001030.11CTR86Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
YKL193C2.970.001500.01SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YAL038W2.960.001540.02CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YDR196C2.940.001630.07CAB5Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
YOL034W2.870.002040.11SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YMR240C2.760.002910.04CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YPL233W2.720.003280.14NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YGR103W2.580.005000.06NOP7Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
YIR011C2.520.005870.06STS1Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation
YDR081C2.460.006890.03PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YKL095W2.430.007502.84E-4YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR455C_d14.701.86E-49YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YPL101W8.229.87E-17ELP4Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
YML071C8.006.04E-16COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR320C7.832.50E-15SWA2Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
YJR053W6.455.42E-11BFA1Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis
YHR120W5.715.67E-9MSH1DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
YOL114C_p5.422.92E-8YOL114C_pPutative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene
YDR439W5.267.19E-8LRS4Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
YGR111W_p5.012.76E-7YGR111W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YJL004C4.943.91E-7SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YDL020C4.934.02E-7RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YJL154C4.904.86E-7VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YKR026C4.885.34E-7GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YJR033C4.846.44E-7RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YPL212C4.808.12E-7PUS1tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA

GO enrichment analysis for SGTC_697
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2125.14E-61SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0502793Golgi
0.2001.46E-54SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.666667amide catabolism
0.1756.03E-42SGTC_4982,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 150.0 μMICCB bioactive library23740.0714286Golgi
0.1661.38E-37SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.132353Golgi
0.1661.47E-37SGTC_6941315-0438 43.2 μMChemDiv (Drug-like library)67931540.180723
0.1441.02E-28SGTC_15956-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 67.5 μMTimTec (Pure natural product library)6887880.102564
0.1432.37E-28SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.15625
0.1423.73E-28SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.0921053NEO1
0.1381.51E-26SGTC_9871497-1013 404.0 μMChemDiv (Drug-like library)16764350.6
0.1374.37E-26SGTC_3660181-0206 4.3 μMChemDiv (Drug-like library)2748740.0677966Golgi
0.1374.97E-26SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.314286Golgi
0.1352.28E-25SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.134328Golgi
0.1337.81E-25SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.0625Golgi
0.1331.37E-24SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.1875Golgi
0.1321.72E-24SGTC_389tpck 16.7 μMMiscellaneous4396470.135135

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2151287-0124461.5 μM0.7924535560863ChemDiv (Drug-like library)433.363487.0303
SGTC_3371315-0038354 μM0.6666671626437ChemDiv (Drug-like library)423.357547.6103amide catabolism
SGTC_9871497-1013404 μM0.61676435ChemDiv (Drug-like library)362.87526.58603
SGTC_7360958-025492.6 μM0.4848486812352ChemDiv (Drug-like library)453.351584.73515Golgi
SGTC_9641287-010719.4 μM0.45454551516812ChemDiv (Drug-like library)435.33635.47814cell wall
SGTC_6320781-23534.65 μM0.388066811515ChemDiv (Drug-like library)443.744084.92914Golgi
SGTC_840443-0269128.82 μM0.3606566150058ChemDiv (Drug-like library)266.294564.16703
SGTC_24005203494178.7 μM0.366777674Miscellaneous399.398784.55216RSC complex & mRNA processing
SGTC_7381315-000372.4 μM0.3521136251474ChemDiv (Drug-like library)372.846886.38803
SGTC_8830850-024524.6 μM0.3333336823229ChemDiv (Drug-like library)409.299025.11814cell wall