1124-0212

N-[(E)-benzylideneamino]-2,4-dichlorobenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_705
Screen concentration 310.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 9554485
SMILES C1=CC=C(C=C1)C=NNC(=O)C2=C(C=C(C=C2)Cl)Cl
Standardized SMILES Clc1ccc(C(=O)NN=Cc2ccccc2)c(Cl)c1
Molecular weight 293.148
ALogP 4.01
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.57
% growth inhibition (Hom. pool) 8.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9554485
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:-3.66|P-value:1.29E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CAB5(YDR196C)|FD-Score:3.87|P-value:5.51E-5|Clearance:0.25||SGD DESC:Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies Gene:CBF5(YLR175W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.1||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CCT3(YJL014W)|FD-Score:3.36|P-value:3.89E-4|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC123(YLR215C)|FD-Score:4.89|P-value:5.08E-7|Clearance:0.37||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:GCD7(YLR291C)|FD-Score:4|P-value:3.23E-5|Clearance:0.09||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GNA1(YFL017C)|FD-Score:5.79|P-value:3.60E-9|Clearance:0.9||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:KEG1(YFR042W)|FD-Score:3.88|P-value:5.22E-5|Clearance:0.01||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:LAS17(YOR181W)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.05||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:NUG1(YER006W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.03||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:OST2(YOR103C)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.04||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RET2(YFR051C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.15||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB7(YDR404C)|FD-Score:-3.1|P-value:9.67E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC53(YDL150W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RSC9(YML127W)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC13(YLR208W)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC24(YIL109C)|FD-Score:3.18|P-value:7.34E-4|Clearance:0.03||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SGV1(YPR161C)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.17||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SOF1(YLL011W)|FD-Score:3.91|P-value:4.65E-5|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPC3(YLR066W)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.02||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:WRS1(YOL097C)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.2||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YBL077W(YBL077W_d)|FD-Score:4.52|P-value:3.05E-6|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:ZIM17(YNL310C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:BET4(YJL031C)|FD-Score:-3.66|P-value:1.29E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CAB5(YDR196C)|FD-Score:3.87|P-value:5.51E-5|Clearance:0.25||SGD DESC:Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies Gene:CBF5(YLR175W)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.1||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CCT3(YJL014W)|FD-Score:3.36|P-value:3.89E-4|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC123(YLR215C)|FD-Score:4.89|P-value:5.08E-7|Clearance:0.37||SGD DESC:Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein Gene:GCD7(YLR291C)|FD-Score:4|P-value:3.23E-5|Clearance:0.09||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GNA1(YFL017C)|FD-Score:5.79|P-value:3.60E-9|Clearance:0.9||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:KEG1(YFR042W)|FD-Score:3.88|P-value:5.22E-5|Clearance:0.01||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:LAS17(YOR181W)|FD-Score:3.15|P-value:8.04E-4|Clearance:0.05||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:NUG1(YER006W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.03||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:OST2(YOR103C)|FD-Score:4.04|P-value:2.72E-5|Clearance:0.04||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RET2(YFR051C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.15||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB7(YDR404C)|FD-Score:-3.1|P-value:9.67E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC53(YDL150W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RSC9(YML127W)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC13(YLR208W)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC24(YIL109C)|FD-Score:3.18|P-value:7.34E-4|Clearance:0.03||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SGV1(YPR161C)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.17||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SOF1(YLL011W)|FD-Score:3.91|P-value:4.65E-5|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SPC3(YLR066W)|FD-Score:3.3|P-value:4.81E-4|Clearance:0.02||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:WRS1(YOL097C)|FD-Score:4.24|P-value:1.11E-5|Clearance:0.2||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YBL077W(YBL077W_d)|FD-Score:4.52|P-value:3.05E-6|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:ZIM17(YNL310C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9554485
Download HOP data (tab-delimited text)  (excel)
Gene:APS1(YLR170C)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:AQR1(YNL065W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:BNR1(YIL159W)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CAX4(YGR036C)|FD-Score:-4.94|P-value:3.87E-7||SGD DESC:Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation Gene:CBP3(YPL215W)|FD-Score:5.63|P-value:8.86E-9||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CHA4(YLR098C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:CLU1(YMR012W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX15(YER141W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:EGD1(YPL037C)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELM1(YKL048C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERV14(YGL054C)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GDH3(YAL062W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GRX8(YLR364W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:GSM1(YJL103C)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:GUF1(YLR289W)|FD-Score:5.43|P-value:2.79E-8||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HHF1(YBR009C)|FD-Score:-4.43|P-value:4.69E-6||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HHT2(YNL031C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HMF1(YER057C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HNT2(YDR305C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:KTR7(YIL085C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MCH1(YDL054C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MRPL16(YBL038W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MYO3(YKL129C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:OCA1(YNL099C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OST6(YML019W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PAF1(YBR279W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCT1(YGR202C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity Gene:PDR8(YLR266C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PEX11(YOL147C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PHO86(YJL117W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIG1(YLR273C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PIG2(YIL045W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PKH3(YDR466W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRS4(YBL068W)|FD-Score:6.55|P-value:2.94E-11||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RKM1(YPL208W)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPL20A(YMR242C)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RRT5(YFR032C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSA3(YLR221C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SAP30(YMR263W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAP4(YGL229C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SLH1(YGR271W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNZ2(YNL333W)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRO7(YPR032W)|FD-Score:6.13|P-value:4.35E-10||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:UPF3(YGR072W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VBA5(YKR105C_p)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:YBL010C(YBL010C_p)|FD-Score:4.85|P-value:6.16E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBL065W(YBL065W_d)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YCR043C(YCR043C_p)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR109C(YDR109C_p)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Putative kinase Gene:YDR220C(YDR220C_d)|FD-Score:3.74|P-value:9.33E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL086C(YIL086C_d)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR045C(YKR045C_p)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR049C(YLR049C_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function Gene:YLR281C(YLR281C_p)|FD-Score:3.77|P-value:8.20E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YLR422W(YLR422W_p)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YMR206W(YMR206W_p)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Putative protein of unknown function Gene:YNL324W(YNL324W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:APS1(YLR170C)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:AQR1(YNL065W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:BNR1(YIL159W)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CAX4(YGR036C)|FD-Score:-4.94|P-value:3.87E-7||SGD DESC:Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation Gene:CBP3(YPL215W)|FD-Score:5.63|P-value:8.86E-9||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:CHA4(YLR098C)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain Gene:CLU1(YMR012W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COX15(YER141W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:EGD1(YPL037C)|FD-Score:3.9|P-value:4.77E-5||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELM1(YKL048C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERV14(YGL054C)|FD-Score:-4.16|P-value:1.57E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GDH3(YAL062W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GRX8(YLR364W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:GSM1(YJL103C)|FD-Score:-3.83|P-value:6.43E-5||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:GUF1(YLR289W)|FD-Score:5.43|P-value:2.79E-8||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HHF1(YBR009C)|FD-Score:-4.43|P-value:4.69E-6||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HHT2(YNL031C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HMF1(YER057C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:HNT2(YDR305C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:KTR7(YIL085C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:MCH1(YDL054C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MRPL16(YBL038W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL49(YJL096W)|FD-Score:-3.31|P-value:4.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MYO3(YKL129C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization Gene:OCA1(YNL099C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Putative protein tyrosine phosphatase, required for cell cycle arrest in response to oxidative damage of DNA Gene:OST6(YML019W)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PAF1(YBR279W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCT1(YGR202C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity Gene:PDR8(YLR266C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PEX11(YOL147C)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PHO86(YJL117W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIG1(YLR273C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase Gene:PIG2(YIL045W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PKH3(YDR466W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PRS4(YBL068W)|FD-Score:6.55|P-value:2.94E-11||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:RAD33(YML011C)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RKM1(YPL208W)|FD-Score:-3.28|P-value:5.28E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RPL20A(YMR242C)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RRT5(YFR032C_p)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:RSA3(YLR221C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:SAP30(YMR263W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAP4(YGL229C)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SLH1(YGR271W)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SNZ2(YNL333W)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SRO7(YPR032W)|FD-Score:6.13|P-value:4.35E-10||SGD DESC:Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication Gene:UPF3(YGR072W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VBA5(YKR105C_p)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:YBL010C(YBL010C_p)|FD-Score:4.85|P-value:6.16E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBL065W(YBL065W_d)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene Gene:YCR043C(YCR043C_p)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR109C(YDR109C_p)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Putative kinase Gene:YDR220C(YDR220C_d)|FD-Score:3.74|P-value:9.33E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL086C(YIL086C_d)|FD-Score:3.89|P-value:5.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR045C(YKR045C_p)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR049C(YLR049C_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative protein of unknown function Gene:YLR281C(YLR281C_p)|FD-Score:3.77|P-value:8.20E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YLR422W(YLR422W_p)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YMR206W(YMR206W_p)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:Putative protein of unknown function Gene:YNL324W(YNL324W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFL017C5.793.60E-90.90GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YLR215C4.895.08E-70.37CDC123Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
YBL077W_d4.523.05E-60.28YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YOL097C4.241.11E-50.20WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YOR103C4.042.72E-50.04OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YLR291C4.003.23E-50.09GCD7Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YLL011W3.914.65E-50.03SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YFR042W3.885.22E-50.01KEG1Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability
YDR196C3.875.51E-50.25CAB5Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
YER006W3.621.50E-40.03NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YFR051C3.591.65E-40.15RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YLR208W3.442.95E-40.08SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YJL014W3.363.89E-40.01CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNL310C3.354.09E-40.04ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YLR066W3.304.81E-40.02SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL068W6.552.94E-11PRS45-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
YPR032W6.134.35E-10SRO7Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
YPL215W5.638.86E-9CBP3Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
YLR289W5.432.79E-8GUF1Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
YLR170C5.305.65E-8APS1Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
YBL010C_p4.856.16E-7YBL010C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
YOL147C4.405.49E-6PEX11Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
YNL333W4.279.69E-6SNZ2Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p
YLR273C4.241.13E-5PIG1Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase
YKL048C4.072.35E-5ELM1Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
YMR206W_p3.973.54E-5YMR206W_pPutative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
YCR043C_p3.963.75E-5YCR043C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
YLR098C3.934.19E-5CHA4DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
YNL146C-A_p3.914.54E-5YNL146C-A_pPutative protein of unknown function
YPL037C3.904.77E-5EGD1Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b

GO enrichment analysis for SGTC_705
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0791.34E-9SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.163934
0.0681.68E-7SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.0967742
0.0655.56E-7SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.15625
0.0621.57E-6SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.169492
0.0612.92E-6SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.127907calcium & mitochondrial duress
0.0594.90E-6SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.056338
0.0589.22E-6SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.116667
0.0571.07E-5SGTC_2491paeonol 100.0 μMMicrosource (Natural product library)110920.173077
0.0561.78E-5SGTC_592k241-0073 46.5 μMChemDiv (Drug-like library)42301580.0461538
0.0534.14E-5SGTC_8740599-0792 117.0 μMChemDiv (Drug-like library)7653050.169811
0.0534.62E-5SGTC_2074185-0010 4.5 μMChemDiv (Drug-like library)37617530.0217391endomembrane recycling
0.0534.95E-5SGTC_1555citronellol 128.0 μMTimTec (Pure natural product library)88420.0363636
0.0526.79E-5SGTC_12490481-0015 10.2 μMChemDiv (Drug-like library)42275770.152542
0.0518.31E-5SGTC_31089121785 49.5 μMChembridge (Drug-like library)171402710.181818
0.0501.04E-4SGTC_1072oxiconazole 14.9 nMNIH Clinical Collection473160.25ergosterol depletion effects on membrane

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_24605552655174.22 μM0.5106385401989Miscellaneous259.6912.62513amide catabolism
SGTC_21065357045167.29 μM0.44668842Chembridge (Fragment library)281.13733.01412
SGTC_130199-008765 μM0.424589825ChemDiv (Drug-like library)354.229326.15902ERG2
SGTC_80354-000170 μM0.4038464993152ChemDiv (Drug-like library)354.229326.15902
SGTC_8820843-0131351 μM0.4038465425421ChemDiv (Drug-like library)278.757243.33613
SGTC_7600568-072582.9 μM0.3921573916171ChemDiv (Drug-like library)380.010345.16613
SGTC_24635473429200 μM0.3921575399405Miscellaneous273.717582.23113ERG2
SGTC_12011889-337245.9 μM0.3888895392199ChemDiv (Drug-like library)282.337023.49113Golgi
SGTC_8273253-0474693 μM0.3818185402065ChemDiv (Drug-like library)288.728922.66113
SGTC_2942905277762.25 μM0.386469503Chembridge (Drug-like library)284.1131234.14912