3807-1509

2-prop-2-enylsulfanyl-5,6,7,8-tetrahydro-[1]benzothiolo[2,3-d]pyrimidin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_709
Screen concentration 243.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1886677
SMILES C=CCSC1=NC(=C2C3=C(CCCC3)SC2=N1)N
Standardized SMILES Nc1nc(SCC=C)nc2sc3CCCCc3c12
Molecular weight 277.4083
ALogP 3.97
H-bond donor count 1
H-bond acceptor count 5
Response signature RSC complex & mRNA processing

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.62
% growth inhibition (Hom. pool) 11.4


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1886677
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:8.37|P-value:3.00E-17|Clearance:0.98||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP7(YPR034W)|FD-Score:4.61|P-value:1.99E-6|Clearance:0.09||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:6.34|P-value:1.16E-10|Clearance:0.38||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.01||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CIA2(YHR122W)|FD-Score:3.35|P-value:3.99E-4|Clearance:0.04||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:DAM1(YGR113W)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.07||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:FCP1(YMR277W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.04||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FHL1(YPR104C)|FD-Score:7.39|P-value:7.52E-14|Clearance:0.07||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:INN1(YNL152W)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.08||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:IQG1(YPL242C)|FD-Score:18|P-value:4.49E-73|Clearance:5.38||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MED6(YHR058C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0.22||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MED7(YOL135C)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MYO2(YOR326W)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:NUP192(YJL039C)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.01||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:RPB11(YOL005C)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.02||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPB3(YIL021W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.13||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:7.31|P-value:1.30E-13|Clearance:0.98||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC82(YPR190C)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.15||SGD DESC:RNA polymerase III subunit C82 Gene:RPO31(YOR116C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.01||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RSC58(YLR033W)|FD-Score:18.2|P-value:3.10E-74|Clearance:5.38||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:9.84|P-value:3.92E-23|Clearance:0.41||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:9.43|P-value:2.10E-21|Clearance:1.06||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC31(YDL195W)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.05||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SFH1(YLR321C)|FD-Score:4.01|P-value:2.99E-5|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD3(YLR147C)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC105(YGL093W)|FD-Score:5.96|P-value:1.28E-9|Clearance:1.35||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPP382(YLR424W)|FD-Score:4.52|P-value:3.09E-6|Clearance:0.06||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:STH1(YIL126W)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.01||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFC3(YAL001C)|FD-Score:4.46|P-value:4.09E-6|Clearance:0.45||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YDL152W(YDL152W_d)|FD-Score:3.39|P-value:3.47E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YTH1(YPR107C)|FD-Score:12.7|P-value:4.57E-37|Clearance:2.83||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:8.37|P-value:3.00E-17|Clearance:0.98||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP7(YPR034W)|FD-Score:4.61|P-value:1.99E-6|Clearance:0.09||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:6.34|P-value:1.16E-10|Clearance:0.38||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:3.31|P-value:4.61E-4|Clearance:0.01||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CIA2(YHR122W)|FD-Score:3.35|P-value:3.99E-4|Clearance:0.04||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:DAM1(YGR113W)|FD-Score:3.23|P-value:6.19E-4|Clearance:0.07||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:FCP1(YMR277W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.04||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FHL1(YPR104C)|FD-Score:7.39|P-value:7.52E-14|Clearance:0.07||SGD DESC:Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants Gene:INN1(YNL152W)|FD-Score:3.52|P-value:2.16E-4|Clearance:0.08||SGD DESC:Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis Gene:IQG1(YPL242C)|FD-Score:18|P-value:4.49E-73|Clearance:5.38||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MED6(YHR058C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0.22||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MED7(YOL135C)|FD-Score:3.43|P-value:2.99E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MYO2(YOR326W)|FD-Score:-4.23|P-value:1.15E-5|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:NUP192(YJL039C)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.01||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:RPB11(YOL005C)|FD-Score:3.41|P-value:3.22E-4|Clearance:0.02||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPB3(YIL021W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.13||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:7.31|P-value:1.30E-13|Clearance:0.98||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC82(YPR190C)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.15||SGD DESC:RNA polymerase III subunit C82 Gene:RPO31(YOR116C)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.01||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RSC58(YLR033W)|FD-Score:18.2|P-value:3.10E-74|Clearance:5.38||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:9.84|P-value:3.92E-23|Clearance:0.41||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:9.43|P-value:2.10E-21|Clearance:1.06||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC31(YDL195W)|FD-Score:3.3|P-value:4.89E-4|Clearance:0.05||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SFH1(YLR321C)|FD-Score:4.01|P-value:2.99E-5|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SMD3(YLR147C)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:SPC105(YGL093W)|FD-Score:5.96|P-value:1.28E-9|Clearance:1.35||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SPP382(YLR424W)|FD-Score:4.52|P-value:3.09E-6|Clearance:0.06||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:STH1(YIL126W)|FD-Score:3.24|P-value:5.92E-4|Clearance:0.01||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFC3(YAL001C)|FD-Score:4.46|P-value:4.09E-6|Clearance:0.45||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:YDL152W(YDL152W_d)|FD-Score:3.39|P-value:3.47E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YTH1(YPR107C)|FD-Score:12.7|P-value:4.57E-37|Clearance:2.83||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1886677
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADE3(YGR204W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM46(YHR199C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARO2(YGL148W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:CIN8(YEL061C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COQ6(YGR255C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:FSH2(YMR222C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GEX2(YKR106W)|FD-Score:-4.31|P-value:8.33E-6||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:HMT1(YBR034C)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HSL1(YKL101W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IDP1(YDL066W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC4(YDR540C)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MAG2(YLR427W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MSC1(YML128C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NPC2(YDL046W)|FD-Score:7.88|P-value:1.63E-15||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:NTH1(YDR001C)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:PEX29(YDR479C)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PRM10(YJL108C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PTR2(YKR093W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RPL20A(YMR242C)|FD-Score:6.37|P-value:9.65E-11||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RSM28(YDR494W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RXT3(YDL076C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SET5(YHR207C)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SLX1(YBR228W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SNO2(YNL334C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SRO9(YCL037C)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:5.55|P-value:1.46E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP4(YDL048C)|FD-Score:6.46|P-value:5.38E-11||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TSA1(YML028W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:TUB3(YML124C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:YBL055C(YBL055C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YER181C(YER181C_d)|FD-Score:4.81|P-value:7.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YGR137W(YGR137W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR036C(YLR036C_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR345W(YLR345W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:ZWF1(YNL241C)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AAD6(YFL056C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADE3(YGR204W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM46(YHR199C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARO2(YGL148W)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:CIN8(YEL061C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:COQ6(YGR255C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS) Gene:FSH2(YMR222C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GEX2(YKR106W)|FD-Score:-4.31|P-value:8.33E-6||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:HMT1(YBR034C)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HSL1(YKL101W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IDP1(YDL066W)|FD-Score:4.53|P-value:2.93E-6||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC4(YDR540C)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:MAG2(YLR427W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MSC1(YML128C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NPC2(YDL046W)|FD-Score:7.88|P-value:1.63E-15||SGD DESC:Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes Gene:NTH1(YDR001C)|FD-Score:-3.43|P-value:2.96E-4||SGD DESC:Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication Gene:PEX29(YDR479C)|FD-Score:4.47|P-value:3.91E-6||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PEX30(YLR324W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PRM10(YJL108C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PTR2(YKR093W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RPL20A(YMR242C)|FD-Score:6.37|P-value:9.65E-11||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RSM28(YDR494W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RXT3(YDL076C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SET5(YHR207C)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SLX1(YBR228W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SNO2(YNL334C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SRO9(YCL037C)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:5.55|P-value:1.46E-8||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP4(YDL048C)|FD-Score:6.46|P-value:5.38E-11||SGD DESC:Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TLG2(YOL018C)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:4.15|P-value:1.70E-5||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TSA1(YML028W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:TUB3(YML124C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:YBL055C(YBL055C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YER181C(YER181C_d)|FD-Score:4.81|P-value:7.46E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YGR137W(YGR137W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR036C(YLR036C_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR345W(YLR345W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YMR262W(YMR262W_p)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:ZWF1(YNL241C)|FD-Score:-3.28|P-value:5.25E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W18.203.10E-745.38RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YPL242C18.004.49E-735.38IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YPR107C12.704.57E-372.83YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YFR037C9.843.92E-230.41RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YML127W9.432.10E-211.06RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YNR016C8.373.00E-170.98ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YPR104C7.397.52E-140.07FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
YDR404C7.311.30E-130.98RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YMR033W6.341.16E-100.38ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YGL093W5.961.28E-91.35SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YPR034W4.611.99E-60.09ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR424W4.523.09E-60.06SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YAL001C4.464.09E-60.45TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YLR321C4.012.99E-50.10SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YHR058C3.914.64E-50.22MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL046W7.881.63E-15NPC2Functional homolog of human NPC2/He1, which is a cholesterol-binding protein whose deficiency causes Niemann-Pick type C2 disease involving retention of cholesterol in lysosomes
YDL048C6.465.38E-11STP4Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress
YMR242C6.379.65E-11RPL20ARibosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
YDR293C5.551.46E-8SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YLR324W5.181.11E-7PEX30Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
YER181C_d4.817.46E-7YER181C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies
YDL066W4.532.93E-6IDP1Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
YDR479C4.473.91E-6PEX29Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
YHR207C4.356.73E-6SET5Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
YOR091W4.151.70E-5TMA46Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
YDL076C4.121.90E-5RXT3Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
YML028W3.963.72E-5TSA1Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
YDR540C3.914.52E-5IRC4Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YGR204W3.875.45E-5ADE3Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
YCL037C3.836.51E-5SRO9Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_709
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4760SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0985916RSC complex & mRNA processing
0.4640SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0526316RSC complex & mRNA processing
0.4233.34E-255SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0540541RSC complex & mRNA processing
0.4214.07E-252SGTC_11333473-0247 50.0 μMChemDiv (Drug-like library)7113110.45614RSC complex & mRNA processing
0.4136.50E-242SGTC_23965342950 42.8 μMMiscellaneous18866771RSC complex & mRNA processing
0.4101.12E-238SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.031746RSC complex & mRNA processing
0.3896.18E-213SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.0533333RSC complex & mRNA processing
0.3852.09E-207SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.0277778RSC complex & mRNA processing
0.3752.81E-196SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0606061RSC complex & mRNA processing
0.3642.11E-184SGTC_14934348-0038 111.0 μMChemDiv (Drug-like library)8004240.0416667RSC complex & mRNA processing
0.3642.40E-184SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.025974RSC & ERG11
0.3613.03E-181SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.015625RSC complex & mRNA processing
0.3417.30E-161SGTC_12290139-0193 12.2 μMChemDiv (Drug-like library)30907520.0909091RSC complex & mRNA processing
0.3381.02E-157SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0606061
0.3045.85E-127SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.015625RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2396534295042.78 μM11886677Miscellaneous277.40833.96715RSC complex & mRNA processing
SGTC_24036032726181.2 μM0.6222222881040Miscellaneous265.39763.80615
SGTC_11333473-024750 μM0.45614711311ChemDiv (Drug-like library)308.422343.35625RSC complex & mRNA processing
SGTC_8184072-270753.5 μM0.4375882871ChemDiv (Drug-like library)272.40844.35313
SGTC_8720588-0139234 μM0.3275862047100ChemDiv (Drug-like library)289.395862.8405
SGTC_14083909-797648.8 μM0.312524152374ChemDiv (Drug-like library)315.456284.12215Golgi
SGTC_5953909-800373.7 μM0.309091699517ChemDiv (Drug-like library)259.369883.65704
SGTC_1472k841-0093193 μM0.301587X1472ChemDiv (Drug-like library)345.459113.21750
SGTC_14103909-810677.9 μM0.2857141039210ChemDiv (Drug-like library)383.50714.6716
SGTC_14113909-8223150 μM0.277778X1411ChemDiv (Drug-like library)468.656176.19241