r015-0009

2-methoxy-6-[[3-[(5-methoxy-6-oxocyclohexa-2,4-dien-1-ylidene)methylamino]propylamino]methylidene]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_712
Screen concentration 80.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 6813872
SMILES COC1=CC=CC(=CNCCCNC=C2C=CC=C(C2=O)OC)C1=O
Standardized SMILES COc1cccc(C=NCCCN=Cc2cccc(OC)c2O)c1O
Molecular weight 342.389
ALogP -0.25
H-bond donor count 2
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.96
% growth inhibition (Hom. pool) 11.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6813872
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.2|P-value:6.99E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP9(YMR033W)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ERG11(YHR007C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.2||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRS1(YLR060W)|FD-Score:23.9|P-value:8.57E-127|Clearance:8.95||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:FRS2(YFL022C)|FD-Score:6.22|P-value:2.49E-10|Clearance:1.6||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:HEM3(YDL205C)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.16||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HSH49(YOR319W)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.14||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSG1(YGL099W)|FD-Score:-3.21|P-value:6.74E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:PUP1(YOR157C)|FD-Score:4.62|P-value:1.88E-6|Clearance:0.65||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RPB8(YOR224C)|FD-Score:3.74|P-value:9.08E-5|Clearance:0.05||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO31(YOR116C)|FD-Score:15|P-value:5.45E-51|Clearance:8.75||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SUI2(YJR007W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.11||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFC1(YBR123C)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.23||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UGP1(YKL035W)|FD-Score:-3.16|P-value:7.95E-4|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP15(YMR093W)|FD-Score:3.98|P-value:3.49E-5|Clearance:0.01||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR114C(YGR114C_d)|FD-Score:-4.11|P-value:1.95E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ACT1(YFL039C)|FD-Score:-3.2|P-value:6.99E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP9(YMR033W)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.02||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ERG11(YHR007C)|FD-Score:3.42|P-value:3.11E-4|Clearance:0.2||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG12(YMR208W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRS1(YLR060W)|FD-Score:23.9|P-value:8.57E-127|Clearance:8.95||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:FRS2(YFL022C)|FD-Score:6.22|P-value:2.49E-10|Clearance:1.6||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:HEM3(YDL205C)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.16||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HSH49(YOR319W)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.14||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LSG1(YGL099W)|FD-Score:-3.21|P-value:6.74E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:PUP1(YOR157C)|FD-Score:4.62|P-value:1.88E-6|Clearance:0.65||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RPB8(YOR224C)|FD-Score:3.74|P-value:9.08E-5|Clearance:0.05||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPO31(YOR116C)|FD-Score:15|P-value:5.45E-51|Clearance:8.75||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SUI2(YJR007W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.11||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TFC1(YBR123C)|FD-Score:3.97|P-value:3.59E-5|Clearance:0.23||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UGP1(YKL035W)|FD-Score:-3.16|P-value:7.95E-4|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP15(YMR093W)|FD-Score:3.98|P-value:3.49E-5|Clearance:0.01||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR114C(YGR114C_d)|FD-Score:-4.11|P-value:1.95E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6813872
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD6(YPL061W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AVT5(YBL089W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BER1(YLR412W)|FD-Score:-4.3|P-value:8.54E-6||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BUD16(YEL029C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CCW12(YLR110C)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIT1(YNR001C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:6.5|P-value:4.00E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.23|P-value:8.31E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCC1(YCL016C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DSD1(YGL196W)|FD-Score:6.2|P-value:2.86E-10||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ENV10(YLR065C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERG5(YMR015C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FET4(YMR319C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GLN3(YER040W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HMO1(YDR174W)|FD-Score:5.66|P-value:7.42E-9||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSP31(YDR533C)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HXT1(YHR094C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC21(YMR073C)|FD-Score:5.88|P-value:2.08E-9||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LAT1(YNL071W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LDB19(YOR322C)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LST7(YGR057C)|FD-Score:4.35|P-value:6.74E-6||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRPL13(YKR006C)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL20(YKR085C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTG1(YMR097C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NAM2(YLR382C)|FD-Score:6|P-value:9.95E-10||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OPI3(YJR073C)|FD-Score:-4.37|P-value:6.20E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PEP12(YOR036W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PHO86(YJL117W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRD1(YCL057W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RAD18(YCR066W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD27(YKL113C)|FD-Score:5.68|P-value:6.88E-9||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RBG1(YAL036C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RPN4(YDL020C)|FD-Score:6.53|P-value:3.27E-11||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTG2(YGL252C)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTT10(YPL183C)|FD-Score:14.3|P-value:7.53E-47||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:RTT107(YHR154W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SDS3(YIL084C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SDT1(YGL224C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SKG3(YLR187W_p)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SOD1(YJR104C)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO7(YAL009W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSA3(YBL075C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STP1(YDR463W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEA1(YOR337W)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TPN1(YGL186C)|FD-Score:7.69|P-value:7.48E-15||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM7(YBR061C)|FD-Score:9.73|P-value:1.11E-22||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TVP38(YKR088C)|FD-Score:4.86|P-value:5.95E-7||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS21(YOR089C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS35(YJL154C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS65(YLR322W_d)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:WTM2(YOR229W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YCL046W(YCL046W_d)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL118W(YDL118W_p)|FD-Score:7.71|P-value:6.47E-15||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YER034W(YER034W_p)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YIH1(YCR059C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL168W(YIL168W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YKL023W(YKL023W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR150W(YPR150W_d)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:AFT1(YGL071W)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD6(YPL061W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AVT5(YBL089W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BER1(YLR412W)|FD-Score:-4.3|P-value:8.54E-6||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BUD16(YEL029C)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CCW12(YLR110C)|FD-Score:4.36|P-value:6.59E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIT1(YNR001C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:6.5|P-value:4.00E-11||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.23|P-value:8.31E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DCC1(YCL016C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DSD1(YGL196W)|FD-Score:6.2|P-value:2.86E-10||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ENV10(YLR065C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ERG5(YMR015C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:FET4(YMR319C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GLN3(YER040W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HMO1(YDR174W)|FD-Score:5.66|P-value:7.42E-9||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSP31(YDR533C)|FD-Score:-3.76|P-value:8.43E-5||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HXT1(YHR094C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC21(YMR073C)|FD-Score:5.88|P-value:2.08E-9||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LAT1(YNL071W)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LDB19(YOR322C)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LST7(YGR057C)|FD-Score:4.35|P-value:6.74E-6||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MRPL13(YKR006C)|FD-Score:-3.16|P-value:8.01E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL20(YKR085C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MTG1(YMR097C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NAM2(YLR382C)|FD-Score:6|P-value:9.95E-10||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OPI3(YJR073C)|FD-Score:-4.37|P-value:6.20E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PEP12(YOR036W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PHO86(YJL117W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRD1(YCL057W)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:RAD18(YCR066W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD27(YKL113C)|FD-Score:5.68|P-value:6.88E-9||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RBG1(YAL036C)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RPN4(YDL020C)|FD-Score:6.53|P-value:3.27E-11||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTG2(YGL252C)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTT10(YPL183C)|FD-Score:14.3|P-value:7.53E-47||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:RTT107(YHR154W)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SDS3(YIL084C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SDT1(YGL224C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SKG3(YLR187W_p)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SOD1(YJR104C)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPO7(YAL009W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSA3(YBL075C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:STP1(YDR463W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEA1(YOR337W)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:TPN1(YGL186C)|FD-Score:7.69|P-value:7.48E-15||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM7(YBR061C)|FD-Score:9.73|P-value:1.11E-22||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TVP38(YKR088C)|FD-Score:4.86|P-value:5.95E-7||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:VPS21(YOR089C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS35(YJL154C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS65(YLR322W_d)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:WTM2(YOR229W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YCL046W(YCL046W_d)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL118W(YDL118W_p)|FD-Score:7.71|P-value:6.47E-15||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDL119C(YDL119C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YER034W(YER034W_p)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress Gene:YIH1(YCR059C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YIL168W(YIL168W)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YKL023W(YKL023W_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR150W(YPR150W_d)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR060W23.908.57E-1278.95FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YOR116C15.005.45E-518.75RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YFL022C6.222.49E-101.60FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YOR157C4.621.88E-60.65PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YMR093W3.983.49E-50.01UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YBR123C3.973.59E-50.23TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YOR224C3.749.08E-50.05RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YJR007W3.691.11E-40.11SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YDL205C3.581.71E-40.16HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YHR007C3.423.11E-40.20ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YMR033W3.236.29E-40.02ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YOR319W3.216.68E-40.14HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YDR367W3.070.001080.05KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YKL028W3.010.001290.06TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YLR222C2.950.001580.19UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL183C14.307.53E-47RTT10WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
YBR061C9.731.11E-22TRM72'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively
YDL118W_p7.716.47E-15YDL118W_pDubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
YGL186C7.697.48E-15TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YDL020C6.533.27E-11RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGL005C6.504.00E-11COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL196W6.202.86E-10DSD1D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
YLR382C6.009.95E-10NAM2Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
YMR073C5.882.08E-9IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YNR001C5.803.27E-9CIT1Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
YKL113C5.686.88E-9RAD275' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family
YDR174W5.667.42E-9HMO1Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
YEL029C5.581.22E-8BUD16Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
YGL071W5.403.35E-8AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YDR435C5.364.05E-8PPM1Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits

GO enrichment analysis for SGTC_712
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4001.03E-225SGTC_608k048-0125 159.0 μMChemDiv (Drug-like library)68176520.442308
0.2081.39E-58SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.42RNA pol III & RNase P/MRP
0.2041.45E-56SGTC_659k018-0002 107.0 μMChemDiv (Drug-like library)67978790.194444
0.1963.54E-52SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.0847458RNA pol III & RNase P/MRP
0.1887.42E-48SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.157895RNA pol III & RNase P/MRP
0.1851.13E-46SGTC_12480469-0687 58.6 μMChemDiv (Drug-like library)40174820.0625
0.1742.96E-41SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.109091RNA pol III & RNase P/MRP
0.1723.23E-40SGTC_2671danthron 100.0 μMMicrosource (Natural product library)29500.159091DNA damage response
0.1619.91E-36SGTC_597k018-0003 82.8 μMChemDiv (Drug-like library)67862340.191781RNA pol III & RNase P/MRP
0.1483.96E-30SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.108696RNA pol III & RNase P/MRP
0.1432.73E-28SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.15RNA pol III & RNase P/MRP
0.1353.06E-25SGTC_1522st012842 45.6 μMTimTec (Pure natural product library)34910670.097561RNA pol III & RNase P/MRP
0.1241.23E-21SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0769231DNA damage response
0.1241.40E-21SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.161765Golgi
0.1163.25E-19SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.150943RNA pol III & RNase P/MRP

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_519k048-001162.8 μM0.4791676836379ChemDiv (Drug-like library)312.151341.63825
SGTC_608k048-0125159 μM0.4423086817652ChemDiv (Drug-like library)350.164082.16525
SGTC_6781218-217985.9 μM0.426820081ChemDiv (Drug-like library)272.256081.82515RNA pol III & RNase P/MRP
SGTC_12860929-0070131 μM0.4166671420408ChemDiv (Drug-like library)251.258461.68325
SGTC_8900929-000647.2 μM0.41378169ChemDiv (Drug-like library)267.324062.58325
SGTC_6941315-043843.2 μM0.3770496793154ChemDiv (Drug-like library)378.80843.90215
SGTC_5860957-02658.15 μM0.3756752309ChemDiv (Drug-like library)388.41592.83726cell wall
SGTC_5571315-011016.2 μM0.2962966833801ChemDiv (Drug-like library)509.077562.63114Golgi
SGTC_1565sinapyl alcohol95.1 μM0.2954555280507TimTec (Pure natural product library)210.226461.41824
SGTC_2586curcumin80 μM0.2931036610332ICCB bioactive library368.37993.55426iron homeostasis