1488-1073

1-cyclohexyl-1-(4-methylphenyl)sulfonyl-3-[(4-methylphenyl)sulfonylcarbamoyl]urea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_713
Screen concentration 324.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3312542
SMILES CC1=CC=C(C=C1)S(=O)(=O)NC(=O)NC(=O)N(C2CCCCC2)S(=O)(=O)C3=CC=C(C=C3)C
Standardized SMILES Cc1ccc(cc1)S(=O)(=O)NC(=O)NC(=O)N(C2CCCCC2)S(=O)(=O)c3ccc(C)cc3
Molecular weight 493.5963
ALogP 4.49
H-bond donor count 2
H-bond acceptor count 6
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.96
% growth inhibition (Hom. pool) 8.77


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3312542
Download HIP data (tab-delimited text)  (excel)
Gene:PTR3(YFR029W)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.23||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RPL33A(YPL143W)|FD-Score:3.15|P-value:8.24E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RSC8(YFR037C)|FD-Score:-3.92|P-value:4.41E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RVB1(YDR190C)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.07||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC3(YER008C)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.28||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:5.29|P-value:6.13E-8|Clearance:1.43||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF4(YMR005W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.08||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TUB1(YML085C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP13(YLR222C)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.11||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:PTR3(YFR029W)|FD-Score:3.38|P-value:3.63E-4|Clearance:0.23||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:RPL33A(YPL143W)|FD-Score:3.15|P-value:8.24E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RSC8(YFR037C)|FD-Score:-3.92|P-value:4.41E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RVB1(YDR190C)|FD-Score:3.13|P-value:8.60E-4|Clearance:0.07||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC3(YER008C)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.28||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SUI2(YJR007W)|FD-Score:5.29|P-value:6.13E-8|Clearance:1.43||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:TAF4(YMR005W)|FD-Score:3.57|P-value:1.76E-4|Clearance:0.08||SGD DESC:TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate Gene:TUB1(YML085C)|FD-Score:3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP13(YLR222C)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.11||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3312542
Download HOP data (tab-delimited text)  (excel)
Gene:ADH3(YMR083W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ARO3(YDR035W)|FD-Score:5.31|P-value:5.54E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:CIN1(YOR349W)|FD-Score:5.37|P-value:3.86E-8||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:DAL3(YIR032C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIA1(YMR316W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DON1(YDR273W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:DSD1(YGL196W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ELP6(YMR312W)|FD-Score:6.55|P-value:2.90E-11||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG2(YMR202W)|FD-Score:8.51|P-value:8.78E-18||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FMO1(YHR176W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP48(YGR052W_p)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GLO4(YOR040W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GND1(YHR183W)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:ICS2(YBR157C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:KIN3(YAR018C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:LRE1(YCL051W)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MKT1(YNL085W)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNN1(YER001W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MRC1(YCL061C)|FD-Score:-3.99|P-value:3.37E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MSS51(YLR203C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NCE101(YJL205C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NTH2(YBR001C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUP60(YAR002W)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PER1(YCR044C)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PRB1(YEL060C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:RKM3(YBR030W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RRG7(YOR305W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSA1(YPL193W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RTC2(YBR147W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTF1(YGL244W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SBE22(YHR103W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SHE1(YBL031W)|FD-Score:5.44|P-value:2.64E-8||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SKG3(YLR187W_p)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SPO7(YAL009W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:TRP2(YER090W)|FD-Score:6.78|P-value:5.97E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.29|P-value:6.25E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UTH1(YKR042W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VAC7(YNL054W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VBA3(YCL069W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS45(YGL095C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YDR048C(YDR048C_d)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR134C(YDR134C)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YDR193W(YDR193W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL010W(YEL010W_d)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL031W(YKL031W_d)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL058C(YNL058C_p)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YTP1(YNL237W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ADH3(YMR083W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ARO3(YDR035W)|FD-Score:5.31|P-value:5.54E-8||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:CIN1(YOR349W)|FD-Score:5.37|P-value:3.86E-8||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:DAL3(YIR032C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DIA1(YMR316W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DON1(YDR273W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:DSD1(YGL196W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ELP6(YMR312W)|FD-Score:6.55|P-value:2.90E-11||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ERG2(YMR202W)|FD-Score:8.51|P-value:8.78E-18||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FMO1(YHR176W)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP48(YGR052W_p)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GLO4(YOR040W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GND1(YHR183W)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:GYP1(YOR070C)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:ICS2(YBR157C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:KIN3(YAR018C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:LRE1(YCL051W)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MKT1(YNL085W)|FD-Score:-3.83|P-value:6.46E-5||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNN1(YER001W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MRC1(YCL061C)|FD-Score:-3.99|P-value:3.37E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MSS51(YLR203C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis Gene:NCE101(YJL205C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NTH2(YBR001C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:NUP60(YAR002W)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PER1(YCR044C)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PRB1(YEL060C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:RKM3(YBR030W)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RRG7(YOR305W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSA1(YPL193W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RTC2(YBR147W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RTF1(YGL244W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SBE22(YHR103W)|FD-Score:6.31|P-value:1.40E-10||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SHE1(YBL031W)|FD-Score:5.44|P-value:2.64E-8||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SKG3(YLR187W_p)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SPO7(YAL009W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:TRP2(YER090W)|FD-Score:6.78|P-value:5.97E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.29|P-value:6.25E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UTH1(YKR042W)|FD-Score:5.18|P-value:1.14E-7||SGD DESC:Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication Gene:VAC7(YNL054W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VBA3(YCL069W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS45(YGL095C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YDR048C(YDR048C_d)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR134C(YDR134C)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YDR193W(YDR193W_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL010W(YEL010W_d)|FD-Score:4.52|P-value:3.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL031W(YKL031W_d)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNL058C(YNL058C_p)|FD-Score:3.1|P-value:9.77E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YTP1(YNL237W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJR007W5.296.13E-81.43SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YER008C3.865.71E-50.28SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YMR005W3.571.76E-40.08TAF4TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
YLR222C3.492.41E-40.11UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YML085C3.383.62E-49.36E-4TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YFR029W3.383.63E-40.23PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YPL143W3.158.24E-40.01RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YDR190C3.138.60E-40.07RVB1ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YBR049C3.070.001080.04REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YNL221C3.030.001220.15POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YPR133C2.880.002000.03SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YIL022W2.850.002170.03TIM44Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
YPL093W2.820.002400.19NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YER006W2.630.004240.06NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YER104W2.580.004980.01RTT105Protein with a role in regulation of Ty1 transposition

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W8.518.78E-18ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YER090W6.785.97E-12TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YMR312W6.552.90E-11ELP6Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
YHR103W6.311.40E-10SBE22Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
YBL031W5.442.64E-8SHE1Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
YOR349W5.373.86E-8CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YDR035W5.315.54E-8ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YKL211C5.296.25E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR134C5.181.12E-7YDR134CHypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication
YKR042W5.181.14E-7UTH1Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
YEL010W_d4.523.12E-6YEL010W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR147W4.032.76E-5RTC2Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter
YDR354W3.739.54E-5TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YLR187W_p3.711.06E-4SKG3_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
YNL054W3.701.08E-4VAC7Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock

GO enrichment analysis for SGTC_713
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2041.25E-56SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0666667
0.2022.12E-55SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0985916
0.2015.51E-55SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0185185calcium & mitochondrial duress
0.2001.71E-54SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.056338
0.1944.70E-51SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.115942
0.1919.44E-50SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.343284
0.1878.76E-48SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0958904
0.1879.93E-48SGTC_22997976368 114.9 μMChembridge (Fragment library)29797180.0428571
0.1862.50E-47SGTC_20945332512 191.7 μMChembridge (Fragment library)7557740.123077
0.1824.80E-45SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0958904
0.1826.17E-45SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.108434
0.1791.45E-43SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.0597015
0.1701.37E-39SGTC_1114scopoletin 1.7 μMTimTec (Natural product library)52804600.0588235
0.1701.92E-39SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.138462ERG2
0.1702.06E-39SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.152778

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13381486-122376 μM0.63444526ChemDiv (Drug-like library)483.503875.3727
SGTC_5831488-109188.9 μM0.5918374303273ChemDiv (Drug-like library)501.575224.69326
SGTC_2851486-132546 μM0.38333322310230ChemDiv (Drug-like library)457.92995.29624
SGTC_13351486-0900202 μM0.369231X1335ChemDiv (Drug-like library)395.516254.1542
SGTC_13391486-132122.7 μM0.3650793469382ChemDiv (Drug-like library)443.903324.8124ERG2
SGTC_2051486-133071.9 μM0.3593754118834ChemDiv (Drug-like library)485.983066.26924
SGTC_1941486-132356.9 μM0.3538465178754ChemDiv (Drug-like library)478.348385.47524
SGTC_9811488-109454.9 μM0.3478263449522ChemDiv (Drug-like library)489.564523.20327
SGTC_13411486-1360280 μM0.3454553976547ChemDiv (Drug-like library)285.31950.99324
SGTC_13371486-101140.3 μM0.34375X1337ChemDiv (Drug-like library)443.903314.8142