0868-0130

3-[(4-acetylphenyl)sulfamoyl]-N-(4-nitrophenyl)benzamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_715
Screen concentration 184.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 2876258
SMILES CC(=O)C1=CC=C(C=C1)NS(=O)(=O)C2=CC=CC(=C2)C(=O)NC3=CC=C(C=C3)[N+](=O)[O-]
Standardized SMILES CC(=O)c1ccc(NS(=O)(=O)c2cccc(c2)C(=O)Nc3ccc(cc3)[N+](=O)[O-])cc1
Molecular weight 439.4412
ALogP 2.74
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.56
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2876258
Download HIP data (tab-delimited text)  (excel)
Gene:BPL1(YDL141W)|FD-Score:-3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:GPI19(YDR437W)|FD-Score:3.91|P-value:4.70E-5|Clearance:0.56||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:RPL17A(YKL180W)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BPL1(YDL141W)|FD-Score:-3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:GPI19(YDR437W)|FD-Score:3.91|P-value:4.70E-5|Clearance:0.56||SGD DESC:Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P Gene:RPL17A(YKL180W)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.3||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2876258
Download HOP data (tab-delimited text)  (excel)
Gene:AEP1(YMR064W)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ANP1(YEL036C)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM1(YPL259C)|FD-Score:5.26|P-value:7.14E-8||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ASP1(YDR321W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:BUD31(YCR063W)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CAP2(YIL034C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:6.84|P-value:3.98E-12||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CDC50(YCR094W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHD1(YER164W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CMK2(YOL016C)|FD-Score:3.82|P-value:6.78E-5||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DFG16(YOR030W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DON1(YDR273W)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:FMP27(YLR454W_p)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE5(YOR384W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPT2(YKR067W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HSL7(YBR133C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IES5(YER092W)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC11(YOR013W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:JNM1(YMR294W)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LRE1(YCL051W)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:NUP84(YDL116W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PAT1(YCR077C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PAU11(YGL261C_p)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDE2(YOR360C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:POA1(YBR022W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PSR1(YLL010C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUF4(YGL014W)|FD-Score:-4.36|P-value:6.37E-6||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RLF2(YPR018W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:SKG3(YLR187W_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SMI1(YGR229C)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:STO1(YMR125W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TDA3(YHR009C_p)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TIP41(YPR040W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:WSS1(YHR134W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YHL044W(YHL044W_p)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YNR064C(YNR064C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:AEP1(YMR064W)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ANP1(YEL036C)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM1(YPL259C)|FD-Score:5.26|P-value:7.14E-8||SGD DESC:Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting Gene:ASP1(YDR321W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:BUD31(YCR063W)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CAP2(YIL034C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:6.84|P-value:3.98E-12||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CDC50(YCR094W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CHD1(YER164W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CMK2(YOL016C)|FD-Score:3.82|P-value:6.78E-5||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:COG6(YNL041C)|FD-Score:-3.16|P-value:7.77E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CYS3(YAL012W)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DFG16(YOR030W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DON1(YDR273W)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:FMP27(YLR454W_p)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE5(YOR384W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GPT2(YKR067W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:HSL7(YBR133C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IES5(YER092W)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC11(YOR013W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:JNM1(YMR294W)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LRE1(YCL051W)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:NUP84(YDL116W)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PAT1(YCR077C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PAU11(YGL261C_p)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PDE2(YOR360C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:POA1(YBR022W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PSR1(YLL010C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:PUF4(YGL014W)|FD-Score:-4.36|P-value:6.37E-6||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RLF2(YPR018W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:SKG3(YLR187W_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SMI1(YGR229C)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:STO1(YMR125W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TDA3(YHR009C_p)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TIP41(YPR040W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:WSS1(YHR134W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YHL044W(YHL044W_p)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YNR064C(YNR064C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR437W3.914.70E-50.56GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P
YKL180W3.344.15E-40.30RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YPL011C3.040.001170.03TAF3TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
YBR123C3.010.001290.07TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YNR016C2.950.001610.04ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YDL148C2.910.001820.03NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YDR396W_d2.870.002030.08YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YML127W2.800.002590.12RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YMR033W2.670.003750.20ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YDL092W2.480.006600.01SRP14Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
YDR404C2.470.006750.00RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YIL021W2.470.006790.05RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YDR398W2.410.007870.02UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YJL104W2.400.008210.09PAM16Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
YDR339C2.300.010600.03FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL043C6.843.98E-12CBR1Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
YCL051W5.413.22E-8LRE1Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication
YPL259C5.267.14E-8APM1Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting
YMR064W5.111.63E-7AEP1Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase
YCR063W5.072.02E-7BUD31Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
YGR229C5.022.61E-7SMI1Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
YGL261C_p4.111.95E-5PAU11_pPutative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2
YNR064C3.904.90E-5YNR064CEpoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
YOL016C3.826.78E-5CMK2Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
YCR077C3.701.08E-4PAT1Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
YCR094W3.641.35E-4CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YLR187W_p3.631.42E-4SKG3_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
YMR125W3.631.42E-4STO1Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
YOR360C3.611.51E-4PDE2High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
YHL044W_p3.462.66E-4YHL044W_pPutative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern

GO enrichment analysis for SGTC_715
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1141.36E-18SGTC_720r052-1644 49.9 μMChemDiv (Drug-like library)14228630.146667TSC3-RPN4
0.0983.65E-14SGTC_29107948405 26.0 μMChembridge (Drug-like library)29713280.103448plasma membrane duress
0.0937.10E-13SGTC_10523253-3998 198.0 μMChemDiv (Drug-like library)54503170.127907
0.0822.44E-10SGTC_1064honokiol 6.8 μMNIH Clinical Collection723030.0547945
0.0765.95E-9SGTC_1609st001574 46.4 μMTimTec (Natural product derivative library)39600120.0736842
0.0691.29E-7SGTC_8870885-0007 9.6 μMChemDiv (Drug-like library)57194580.047619endomembrane recycling
0.0681.60E-7SGTC_7144296-0578 26.1 μMChemDiv (Drug-like library)15897750.15625
0.0654.77E-7SGTC_9861493-0483 84.0 μMChemDiv (Drug-like library)400790.19403
0.0631.17E-6SGTC_27905423970 71.4 μMChembridge (Drug-like library)13762400.0722892endomembrane recycling
0.0604.48E-6SGTC_7064262-0057 46.9 μMChemDiv (Drug-like library)8832980.0571429fatty acid desaturase (OLE1)
0.0596.74E-6SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.0724638heat shock/prefoldin
0.0561.73E-5SGTC_7283030-5151 22.5 μMChemDiv (Drug-like library)31253880.0833333
0.0552.54E-5SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.0526316
0.0552.78E-5SGTC_32299132204 49.5 μMChembridge (Drug-like library)176829240.11236
0.0525.53E-5SGTC_1987st072290 61.9 μMTimTec (Natural product derivative library)54697379NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_990109-0120622.8 μM0.480769759414ChemDiv (Drug-like library)321.126163.25713
SGTC_8080711-0032135 μM0.4482764252555ChemDiv (Drug-like library)370.218382.92115
SGTC_340469-068381.75 μM0.4385963690923ChemDiv (Drug-like library)337.307942.59217SWF1 & branched chain AA biosynthesis
SGTC_2786774361745.45 μM0.436364583674Chembridge (Drug-like library)331.429243.45814
SGTC_10024049-0206172 μM0.4310344662749ChemDiv (Drug-like library)310.3008432.90316
SGTC_8991083-0020478 μM0.390625767123ChemDiv (Drug-like library)306.701143.15714
SGTC_625k213-0058121 μM0.3857143655420ChemDiv (Drug-like library)476.868584.50426translation
SGTC_12590671-013243 μM0.383333X1259ChemDiv (Drug-like library)371.206443.44641
SGTC_21826325192200 μM0.375736248Chembridge (Fragment library)226.273762.35422Golgi
SGTC_6260214-000933 μM0.3728813437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress