1130-0073

1-[[(5-chloropyridin-2-yl)amino]methylidene]naphthalen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_718
Screen concentration 349.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6740020
SMILES C1=CC=C2C(=C1)C=CC(=O)C2=CNC3=NC=C(C=C3)Cl
Standardized SMILES Oc1ccc2ccccc2c1C=Nc3ccc(Cl)cn3
Molecular weight 282.7243
ALogP 3.2
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.72
% growth inhibition (Hom. pool) 7.68


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6740020
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC9(YDL164C)|FD-Score:3.99|P-value:3.37E-5|Clearance:0.23||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CSE1(YGL238W)|FD-Score:-3.13|P-value:8.81E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CUS1(YMR240C)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.03||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:NMD3(YHR170W)|FD-Score:-4.91|P-value:4.52E-7|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUP57(YGR119C)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.39||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PRE8(YML092C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.07||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPC53(YDL150W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:6.13|P-value:4.40E-10|Clearance:1.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMD2(YLR275W)|FD-Score:5.8|P-value:3.32E-9|Clearance:1.19||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:UTP15(YMR093W)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.41||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR124W(YBR124W_d)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.13||SGD DESC:Putative protein of unknown function Gene:YDL196W(YDL196W_d)|FD-Score:-5.5|P-value:1.92E-8|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YKL111C(YKL111C_d)|FD-Score:3.75|P-value:8.68E-5|Clearance:0.04||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1 Gene:CDC19(YAL038W)|FD-Score:-3.17|P-value:7.72E-4|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:CDC9(YDL164C)|FD-Score:3.99|P-value:3.37E-5|Clearance:0.23||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:CSE1(YGL238W)|FD-Score:-3.13|P-value:8.81E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:CUS1(YMR240C)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.03||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:NMD3(YHR170W)|FD-Score:-4.91|P-value:4.52E-7|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NUP57(YGR119C)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.39||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PRE8(YML092C)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.07||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPC53(YDL150W)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:6.13|P-value:4.40E-10|Clearance:1.19||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMD2(YLR275W)|FD-Score:5.8|P-value:3.32E-9|Clearance:1.19||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:UTP15(YMR093W)|FD-Score:4.61|P-value:2.05E-6|Clearance:0.41||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR124W(YBR124W_d)|FD-Score:3.31|P-value:4.64E-4|Clearance:0.13||SGD DESC:Putative protein of unknown function Gene:YDL196W(YDL196W_d)|FD-Score:-5.5|P-value:1.92E-8|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR114C(YGR114C_d)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YKL111C(YKL111C_d)|FD-Score:3.75|P-value:8.68E-5|Clearance:0.04||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene ABF1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6740020
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AFT1(YGL071W)|FD-Score:7.56|P-value:2.08E-14||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO3(YDR035W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BCK1(YJL095W)|FD-Score:5.93|P-value:1.54E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUB2(YMR055C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:COQ3(YOL096C)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:DIP5(YPL265W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DST1(YGL043W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM34(YHL043W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:ERJ5(YFR041C)|FD-Score:3.78|P-value:7.70E-5||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FEN2(YCR028C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FUN26(YAL022C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GAL4(YPL248C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GND2(YGR256W)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HAM1(YJR069C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:4|P-value:3.23E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HOM6(YJR139C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HUA1(YGR268C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IES3(YLR052W)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMD3(YLR432W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:INP2(YMR163C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IRA2(YOL081W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:ISA2(YPR067W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JNM1(YMR294W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:OCA2(YNL056W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPI3(YJR073C)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OXR1(YPL196W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PAF1(YBR279W)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PHO84(YML123C)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PRM5(YIL117C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:RCN1(YKL159C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:ROX3(YBL093C)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL19A(YBR084C-A)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPS24B(YIL069C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RUP1(YOR138C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SRC1(YML034W)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSK1(YLR006C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SVF1(YDR346C)|FD-Score:-4.35|P-value:6.85E-6||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYS1(YJL004C)|FD-Score:6.62|P-value:1.78E-11||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA11(YHR159W_p)|FD-Score:5.29|P-value:6.18E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TFB5(YDR079C-A)|FD-Score:5.28|P-value:6.61E-8||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TFB6(YOR352W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:THI72(YOR192C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOM70(YNL121C)|FD-Score:-4.34|P-value:7.17E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:14.5|P-value:1.08E-47||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSR3(YOR006C)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:URN1(YPR152C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VMA11(YPL234C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VMA8(YEL051W)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS1(YKR001C)|FD-Score:-3.76|P-value:8.38E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR056W(YBR056W_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCR001W(YCR001W_d)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YDR442W(YDR442W_d)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL177W(YGL177W_d)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR228W(YGR228W_d)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YJL163C(YJL163C_p)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL324W(YNL324W_d)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR292C(YOR292C_p)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:AAT2(YLR027C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AFT1(YGL071W)|FD-Score:7.56|P-value:2.08E-14||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO3(YDR035W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BCK1(YJL095W)|FD-Score:5.93|P-value:1.54E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUB2(YMR055C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:COQ3(YOL096C)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:DIP5(YPL265W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:DST1(YGL043W)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM34(YHL043W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:ERJ5(YFR041C)|FD-Score:3.78|P-value:7.70E-5||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FEN2(YCR028C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FUN26(YAL022C)|FD-Score:-4.09|P-value:2.17E-5||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GAL4(YPL248C)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GND2(YGR256W)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HAM1(YJR069C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:4|P-value:3.23E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HOM6(YJR139C)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HUA1(YGR268C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:IES3(YLR052W)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMD3(YLR432W)|FD-Score:3.87|P-value:5.53E-5||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:INP2(YMR163C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IRA2(YOL081W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:ISA2(YPR067W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:JNM1(YMR294W)|FD-Score:-3.31|P-value:4.64E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:OCA2(YNL056W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OPI3(YJR073C)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:OXR1(YPL196W)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PAF1(YBR279W)|FD-Score:3.93|P-value:4.16E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PHO84(YML123C)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PRM5(YIL117C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:RCN1(YKL159C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:ROX3(YBL093C)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL19A(YBR084C-A)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPS24B(YIL069C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RPS9A(YPL081W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RUP1(YOR138C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SRC1(YML034W)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSK1(YLR006C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SVF1(YDR346C)|FD-Score:-4.35|P-value:6.85E-6||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SYS1(YJL004C)|FD-Score:6.62|P-value:1.78E-11||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TDA11(YHR159W_p)|FD-Score:5.29|P-value:6.18E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TFB5(YDR079C-A)|FD-Score:5.28|P-value:6.61E-8||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TFB6(YOR352W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:THI72(YOR192C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOM70(YNL121C)|FD-Score:-4.34|P-value:7.17E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:14.5|P-value:1.08E-47||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSR3(YOR006C)|FD-Score:5.1|P-value:1.73E-7||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:URN1(YPR152C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Putative protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase Gene:VMA11(YPL234C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VMA8(YEL051W)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Subunit D of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis Gene:VPS1(YKR001C)|FD-Score:-3.76|P-value:8.38E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR056W(YBR056W_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YCR001W(YCR001W_d)|FD-Score:4.61|P-value:2.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene Gene:YDR442W(YDR442W_d)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL177W(YGL177W_d)|FD-Score:-4.56|P-value:2.60E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR228W(YGR228W_d)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YJL163C(YJL163C_p)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Putative protein of unknown function Gene:YML122C(YML122C_d)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL203C(YNL203C_d)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL324W(YNL324W_d)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR292C(YOR292C_p)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C6.134.40E-101.19RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR275W5.803.32E-91.19SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YMR093W4.612.05E-60.41UTP15Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGR114C_d4.201.34E-50.21YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YDL164C3.993.37E-50.23CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YKL111C_d3.758.68E-50.04YKL111C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
YDL150W3.721.01E-40.02RPC53RNA polymerase III subunit C53
YGR119C3.701.09E-40.39NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YBR124W_d3.314.64E-40.13YBR124W_dPutative protein of unknown function
YML092C3.197.23E-40.07PRE8Alpha 2 subunit of the 20S proteasome
YMR240C3.119.24E-40.03CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YBR160W3.080.001020.01CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YNL207W3.080.001050.06RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YMR128W3.010.001290.00ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YGR116W3.010.001300.23SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL186C14.501.08E-47TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YGL071W7.562.08E-14AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YJL004C6.621.78E-11SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YJL095W5.931.54E-9BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YML034W5.501.87E-8SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YHR159W_p5.296.18E-8TDA11_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
YDR079C-A5.286.61E-8TFB5Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
YOR006C5.101.73E-7TSR3Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YCR001W_d4.612.03E-6YCR001W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR001W is not an essential gene
YML122C_d4.592.26E-6YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YJR073C4.337.62E-6OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YJR139C4.241.10E-5HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YGR268C4.221.20E-5HUA1Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YMR055C4.201.34E-5BUB2Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
YDR129C4.181.49E-5SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_718
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1263.68E-22SGTC_1466k213-0079 57.5 μMChemDiv (Drug-like library)36579810.106667
0.1195.86E-20SGTC_9373122-0005 46.7 μMChemDiv (Drug-like library)7595350.106061
0.1132.35E-18SGTC_2525harmine 47.9 μMMiscellaneous52809530.0923077ergosterol biosynthesis
0.1095.44E-17SGTC_13441488-0097 29.1 μMChemDiv (Drug-like library)7479660.15873
0.1072.08E-16SGTC_31199125183 49.5 μMChembridge (Drug-like library)226953340.142857
0.1041.10E-15SGTC_2385hr heat shock (37°C) + nsc-638432 8.9 μMMiscellaneous3680900.150685heat shock/prefoldin
0.1041.27E-15SGTC_480382-0018 55.0 μMChemDiv (Drug-like library)7625960.0967742
0.1031.94E-15SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.2heat shock/prefoldin
0.1024.22E-15SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.027027heat shock/prefoldin
0.1001.92E-14SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.0882353RPP1 & pyrimidine depletion
0.0983.47E-14SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.197183Golgi
0.0979.29E-14SGTC_610978-8327 98.6 μMChemDiv (Drug-like library)61500590.0882353ergosterol biosynthesis
0.0961.20E-13SGTC_23307979373 200.0 μMChembridge (Fragment library)18630070.089552260S ribosome export
0.0961.73E-13SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0909091cell wall signaling
0.0944.41E-13SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.0172414cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6441498-119763.3 μM0.4705886741615ChemDiv (Drug-like library)291.343782.86623
SGTC_6671498-104459 μM0.4705886739982ChemDiv (Drug-like library)291.300722.77924RPP1 & pyrimidine depletion
SGTC_7851270-011165.3 μM0.452836741994ChemDiv (Drug-like library)307.300122.53735
SGTC_8282074-0507139 μM0.435484X828ChemDiv (Drug-like library)397.856346.19732RPP1 & pyrimidine depletion
SGTC_780336-0123156.75 μM0.4310346765896ChemDiv (Drug-like library)380.461645.15814
SGTC_940334-0113514.75 μM0.4098366795178ChemDiv (Drug-like library)388.525164.98514
SGTC_11760848-013183.3 μM0.46769040ChemDiv (Drug-like library)414.454725.58514Golgi
SGTC_8981000-084928.3 μM0.3928576751807ChemDiv (Drug-like library)356.374042.72334
SGTC_1280906-298113.37 μM0.379316740226ChemDiv (Drug-like library)296.343682.0124
SGTC_7700389-112167.2 μM0.3571436753108ChemDiv (Drug-like library)464.49373.42529RPP1 & pyrimidine depletion