0744-0168

1-(2,4-dihydroxyphenyl)-2-phenoxyethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_72
Screen concentration 198.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 677577
SMILES C1=CC=C(C=C1)OCC(=O)C2=C(C=C(C=C2)O)O
Standardized SMILES Oc1ccc(C(=O)COc2ccccc2)c(O)c1
Molecular weight 244.2427
ALogP 2.59
H-bond donor count 2
H-bond acceptor count 4
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.83
% growth inhibition (Hom. pool) 16.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 677577
Download HIP data (tab-delimited text)  (excel)
Gene:CAK1(YFL029C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.05||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC27(YBL084C)|FD-Score:-3.3|P-value:4.88E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DAD2(YKR083C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.02||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBF4(YDR052C)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DIB1(YPR082C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:DIS3(YOL021C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GCD6(YDR211W)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.13||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:MET4(YNL103W)|FD-Score:-3.79|P-value:7.56E-5|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MPS2(YGL075C)|FD-Score:-7.48|P-value:3.79E-14|Clearance:0||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:MYO1(YHR023W)|FD-Score:4.59|P-value:2.25E-6|Clearance:0.43||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:RHO1(YPR165W)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.29||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIO2(YNL207W)|FD-Score:3.83|P-value:6.50E-5|Clearance:0.11||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPL25(YOL127W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPR2(YIR015W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.09||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRS1(YOR294W)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.61||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SDO1(YLR022C)|FD-Score:6.2|P-value:2.75E-10|Clearance:0.61||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SDS22(YKL193C)|FD-Score:5.68|P-value:6.60E-9|Clearance:0.61||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:SEC53(YFL045C)|FD-Score:-3.12|P-value:9.02E-4|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:TAF10(YDR167W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.07||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TIF11(YMR260C)|FD-Score:-3.89|P-value:5.11E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF5(YPR041W)|FD-Score:4.16|P-value:1.59E-5|Clearance:0.05||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:UTP4(YDR324C)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YCS4(YLR272C)|FD-Score:5.78|P-value:3.76E-9|Clearance:0.61||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDR413C(YDR413C_d)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YGR073C(YGR073C_d)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:CAK1(YFL029C)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.05||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC27(YBL084C)|FD-Score:-3.3|P-value:4.88E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:DAD2(YKR083C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.02||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DBF4(YDR052C)|FD-Score:-3.48|P-value:2.53E-4|Clearance:0||SGD DESC:Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress Gene:DIB1(YPR082C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:17-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein Gene:DIS3(YOL021C)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.03||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GCD6(YDR211W)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.13||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:MET4(YNL103W)|FD-Score:-3.79|P-value:7.56E-5|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MPS2(YGL075C)|FD-Score:-7.48|P-value:3.79E-14|Clearance:0||SGD DESC:Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p Gene:MYO1(YHR023W)|FD-Score:4.59|P-value:2.25E-6|Clearance:0.43||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:RHO1(YPR165W)|FD-Score:4.11|P-value:1.96E-5|Clearance:0.29||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIO2(YNL207W)|FD-Score:3.83|P-value:6.50E-5|Clearance:0.11||SGD DESC:Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p Gene:RPL25(YOL127W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.02||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPR2(YIR015W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.09||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RPT1(YKL145W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRS1(YOR294W)|FD-Score:5.2|P-value:1.02E-7|Clearance:0.61||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:SDO1(YLR022C)|FD-Score:6.2|P-value:2.75E-10|Clearance:0.61||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SDS22(YKL193C)|FD-Score:5.68|P-value:6.60E-9|Clearance:0.61||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:SEC53(YFL045C)|FD-Score:-3.12|P-value:9.02E-4|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:TAF10(YDR167W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.07||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TIF11(YMR260C)|FD-Score:-3.89|P-value:5.11E-5|Clearance:0||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF5(YPR041W)|FD-Score:4.16|P-value:1.59E-5|Clearance:0.05||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:UTP4(YDR324C)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YCS4(YLR272C)|FD-Score:5.78|P-value:3.76E-9|Clearance:0.61||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YDR413C(YDR413C_d)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YGR073C(YGR073C_d)|FD-Score:3.46|P-value:2.67E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 677577
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADK2(YER170W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AEP1(YMR064W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ALG6(YOR002W)|FD-Score:6.33|P-value:1.21E-10||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APL1(YJR005W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARG82(YDR173C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG3(YNR007C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:ATG31(YDR022C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:BEM3(YPL115C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BST1(YFL025C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD6(YLR319C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CIN2(YPL241C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLC1(YGR167W)|FD-Score:5.75|P-value:4.58E-9||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX14(YML129C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX5A(YNL052W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:CPS1(YJL172W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CTK3(YML112W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCN1(YLR128W)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DMA1(YHR115C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DNF3(YMR162C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:DST1(YGL043W)|FD-Score:9.77|P-value:7.57E-23||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM27(YJR106W)|FD-Score:-4.76|P-value:9.70E-7||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EEB1(YPL095C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EMC1(YCL045C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:ENV9(YOR246C)|FD-Score:7.09|P-value:6.65E-13||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERG2(YMR202W)|FD-Score:7.87|P-value:1.74E-15||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA1(YOR317W)|FD-Score:-6.76|P-value:6.83E-12||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTR1(YML121W)|FD-Score:4.89|P-value:5.06E-7||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GYP5(YPL249C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HXT3(YDR345C)|FD-Score:5.42|P-value:2.96E-8||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IMP2'(YIL154C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC10(YOL015W)|FD-Score:-4.1|P-value:2.09E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST3(YIR005W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:IXR1(YKL032C)|FD-Score:5.87|P-value:2.23E-9||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LTV1(YKL143W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MNT2(YGL257C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRPS16(YPL013C)|FD-Score:3.75|P-value:8.77E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MST1(YKL194C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NBP2(YDR162C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PAR32(YDL173W)|FD-Score:4.79|P-value:8.53E-7||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX29(YDR479C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PIP2(YOR363C)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:POL4(YCR014C)|FD-Score:-6.36|P-value:9.95E-11||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:QCR7(YDR529C)|FD-Score:4.34|P-value:7.03E-6||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:QDR3(YBR043C)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:QRI5(YLR204W)|FD-Score:-5.93|P-value:1.50E-9||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD14(YMR201C)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD16(YBR114W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RHO5(YNL180C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPA49(YNL248C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPL35A(YDL191W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:5.41|P-value:3.12E-8||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:SAC1(YKL212W)|FD-Score:7.02|P-value:1.13E-12||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCP160(YJL080C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SDH2(YLL041C)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SPG4(YMR107W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPO14(YKR031C)|FD-Score:-4.55|P-value:2.71E-6||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPR1(YOR190W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SSH4(YKL124W)|FD-Score:4.85|P-value:6.05E-7||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SUE1(YPR151C)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Mitochondrial protein required for degradation of unstable forms of cytochrome c Gene:TDA2(YER071C_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA5(YLR426W_p)|FD-Score:5.02|P-value:2.58E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THI7(YLR237W)|FD-Score:-4.89|P-value:5.15E-7||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:THP2(YHR167W)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TIM21(YGR033C)|FD-Score:-4.31|P-value:8.03E-6||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TMA16(YOR252W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRP1(YDR007W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBA3(YPR066W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP13(YBL067C)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VPS15(YBR097W)|FD-Score:4.88|P-value:5.36E-7||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.73|P-value:9.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR016W(YCR016W_p)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDL032W(YDL032W_d)|FD-Score:4.28|P-value:9.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:-3.75|P-value:8.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR445C(YDR445C_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER119C-A(YER119C-A_d)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL042C(YGL042C_d)|FD-Score:32.3|P-value:3.33E-229||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGL149W(YGL149W_d)|FD-Score:-4.46|P-value:4.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGL159W(YGL159W_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YIL165C(YIL165C_p)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YKR073C(YKR073C_d)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR111W(YLR111W_d)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR173W(YLR173W_p)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Putative protein of unknown function Gene:YLR282C(YLR282C_d)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YLR311C(YLR311C_d)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function Gene:YLR413W(YLR413W_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML037C(YML037C_p)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL146W(YNL146W_p)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YNL193W(YNL193W_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR053W(YOR053W_d)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR223W(YOR223W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR277C(YOR277C_d)|FD-Score:-4.74|P-value:1.06E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR283W(YOR283W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR014C(YPR014C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR078C(YPR078C_p)|FD-Score:-3.87|P-value:5.50E-5||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPR127W(YPR127W)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ACB1(YGR037C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADK2(YER170W)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AEP1(YMR064W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ALG6(YOR002W)|FD-Score:6.33|P-value:1.21E-10||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APL1(YJR005W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARG82(YDR173C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ATG3(YNR007C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:ATG31(YDR022C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:BEM3(YPL115C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BST1(YFL025C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD6(YLR319C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CIN2(YPL241C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLC1(YGR167W)|FD-Score:5.75|P-value:4.58E-9||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX14(YML129C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX5A(YNL052W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication Gene:CPS1(YJL172W)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CTK3(YML112W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DCN1(YLR128W)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DMA1(YHR115C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DNF3(YMR162C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase Gene:DST1(YGL043W)|FD-Score:9.77|P-value:7.57E-23||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:ECM27(YJR106W)|FD-Score:-4.76|P-value:9.70E-7||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EEB1(YPL095C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EMC1(YCL045C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:ENV9(YOR246C)|FD-Score:7.09|P-value:6.65E-13||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERG2(YMR202W)|FD-Score:7.87|P-value:1.74E-15||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA1(YOR317W)|FD-Score:-6.76|P-value:6.83E-12||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FLC2(YAL053W)|FD-Score:3.77|P-value:8.18E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:GSF2(YML048W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:GTR1(YML121W)|FD-Score:4.89|P-value:5.06E-7||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GYP5(YPL249C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HXT3(YDR345C)|FD-Score:5.42|P-value:2.96E-8||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IMP2'(YIL154C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC10(YOL015W)|FD-Score:-4.1|P-value:2.09E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IST3(YIR005W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:IXR1(YKL032C)|FD-Score:5.87|P-value:2.23E-9||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:LTV1(YKL143W)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MNT2(YGL257C)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MRPS16(YPL013C)|FD-Score:3.75|P-value:8.77E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MST1(YKL194C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NBP2(YDR162C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:PAR32(YDL173W)|FD-Score:4.79|P-value:8.53E-7||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PEX29(YDR479C)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PIP2(YOR363C)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:POL4(YCR014C)|FD-Score:-6.36|P-value:9.95E-11||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:QCR7(YDR529C)|FD-Score:4.34|P-value:7.03E-6||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:QDR3(YBR043C)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:QRI5(YLR204W)|FD-Score:-5.93|P-value:1.50E-9||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD14(YMR201C)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD16(YBR114W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RHO5(YNL180C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPA49(YNL248C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPL35A(YDL191W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:5.41|P-value:3.12E-8||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RRT12(YCR045C)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:SAC1(YKL212W)|FD-Score:7.02|P-value:1.13E-12||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCP160(YJL080C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SDH2(YLL041C)|FD-Score:-3.74|P-value:9.30E-5||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SPG4(YMR107W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPO14(YKR031C)|FD-Score:-4.55|P-value:2.71E-6||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPR1(YOR190W)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SSH4(YKL124W)|FD-Score:4.85|P-value:6.05E-7||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SUE1(YPR151C)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Mitochondrial protein required for degradation of unstable forms of cytochrome c Gene:TDA2(YER071C_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA5(YLR426W_p)|FD-Score:5.02|P-value:2.58E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THI7(YLR237W)|FD-Score:-4.89|P-value:5.15E-7||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:THP2(YHR167W)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TIM21(YGR033C)|FD-Score:-4.31|P-value:8.03E-6||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TMA16(YOR252W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRP1(YDR007W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP3(YKL211C)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBA3(YPR066W)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBP13(YBL067C)|FD-Score:4.61|P-value:2.06E-6||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:VPS15(YBR097W)|FD-Score:4.88|P-value:5.36E-7||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.73|P-value:9.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR016W(YCR016W_p)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YDL032W(YDL032W_d)|FD-Score:4.28|P-value:9.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDL094C(YDL094C_d)|FD-Score:-3.75|P-value:8.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential Gene:YDR445C(YDR445C_d)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER119C-A(YER119C-A_d)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL042C(YGL042C_d)|FD-Score:32.3|P-value:3.33E-229||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGL149W(YGL149W_d)|FD-Score:-4.46|P-value:4.11E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF INO80/YGL150C. Gene:YGL159W(YGL159W_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function; deletion mutant has no detectable phenotype Gene:YIL165C(YIL165C_p)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YKR073C(YKR073C_d)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR111W(YLR111W_d)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR173W(YLR173W_p)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Putative protein of unknown function Gene:YLR282C(YLR282C_d)|FD-Score:-3.31|P-value:4.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YLR311C(YLR311C_d)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Putative protein of unknown function Gene:YLR413W(YLR413W_p)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YML037C(YML037C_p)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL146W(YNL146W_p)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YNL193W(YNL193W_p)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR053W(YOR053W_d)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR223W(YOR223W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR277C(YOR277C_d)|FD-Score:-4.74|P-value:1.06E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR283W(YOR283W)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR014C(YPR014C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR078C(YPR078C_p)|FD-Score:-3.87|P-value:5.50E-5||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:YPR127W(YPR127W)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR022C6.202.75E-100.61SDO1Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
YLR272C5.783.76E-90.61YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YKL193C5.686.60E-90.61SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YOR294W5.201.02E-70.61RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YHR023W4.592.25E-60.43MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YPR041W4.161.59E-50.05TIF5Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
YPR165W4.111.96E-50.29RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YNL207W3.836.50E-50.11RIO2Essential serine kinase involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
YKR083C3.721.00E-40.02DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YFL029C3.691.10E-40.05CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YKL145W3.651.33E-40.04RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YOL127W3.611.55E-40.02RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YIR015W3.581.70E-40.09RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YOL021C3.492.39E-40.03DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YGR073C_d3.462.67E-40.07YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL042C_d32.303.33E-229YGL042C_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
YGL043W9.777.57E-23DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YMR202W7.871.74E-15ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YOR246C7.096.65E-13ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YKL212W7.021.13E-12SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YOR002W6.331.21E-10ALG6Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
YKL032C5.872.23E-9IXR1Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b
YGR167W5.754.58E-9CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YDR345C5.422.96E-8HXT3Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
YHL033C5.413.12E-8RPL8ARibosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
YHR167W5.111.62E-7THP2Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
YGL012W5.062.06E-7ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YBR114W5.052.22E-7RAD16Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
YLR426W_p5.022.58E-7TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YER119C-A_d5.012.69E-7YER119C-A_dDubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2

GO enrichment analysis for SGTC_72
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1089.25E-17SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0804598cell wall signaling
0.1063.68E-16SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.144928ERG2
0.0978.88E-14SGTC_3474534-1311 26.2 μMChemDiv (Drug-like library)28620160.1875RPP1 & pyrimidine depletion
0.0961.21E-13SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0847458ERG2
0.0961.30E-13SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.174603ERG2
0.0961.79E-13SGTC_7891309-1135 70.6 μMChemDiv (Drug-like library)30924240.107692ERG2
0.0936.32E-13SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.1ERG2
0.0936.67E-13SGTC_13391486-1321 22.7 μMChemDiv (Drug-like library)34693820.134328ERG2
0.0881.12E-11SGTC_1080509-0257 621.1 μMChemDiv (Drug-like library)5366240.107143
0.0863.79E-11SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.0634921ERG2
0.0855.60E-11SGTC_2734clemastine 41.6 μMMiscellaneous269870.121212ERG2
0.0841.14E-10SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.151515ERG2
0.0841.27E-10SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.142857Golgi
0.0832.13E-10SGTC_23227413319 38.2 μMChembridge (Fragment library)18404000.0833333ERAD & cell cycle
0.0805.99E-10SGTC_466geldanamycin 89.2 μMICCB bioactive library52883820.0729167RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1935st07453241.6 μM0.52829742TimTec (Natural product derivative library)272.295843.55724
SGTC_2610ononetin44.17 μM0.5259632Microsource (Natural product library)258.269262.76824
SGTC_1836st05564281 μM0.4857148571TimTec (Natural product derivative library)246.21552.26645
SGTC_2521angolensin13.4 μM0.395833821428Microsource (Natural product library)272.295843.23124
SGTC_25772',4'-dihydroxy-4-methoxychalcone15.32 μM0.3877555711223Microsource (Natural product library)270.279963.20124
SGTC_2565isoliquiritigenin100 μM0.386364638278Microsource (Natural product library)256.253382.97534
SGTC_2477596768625.23 μM0.367347776118Miscellaneous270.279962.89614
SGTC_2481547894845.16 μM0.35416777792Miscellaneous270.279962.89614PDR1
SGTC_25483',4'-didesmethyl-5-deshydroxy-3'-ethoxyscleroin19.94 μM0.3529414000243Microsource (Natural product library)274.268662.8435redox potentiating
SGTC_2491paeonol100 μM0.34883711092Microsource (Natural product library)166.17391.31113