r052-1644

2-[(7-chloro-4-nitro-2,1,3-benzoxadiazol-5-yl)sulfanyl]acetic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_720
Screen concentration 49.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 1422863
SMILES C1=C(C2=NON=C2C(=C1SCC(=O)O)[N+](=O)[O-])Cl
Standardized SMILES OC(=O)CSc1cc(Cl)c2nonc2c1[N+](=O)[O-]
Molecular weight 289.6525
ALogP 1.98
H-bond donor count 1
H-bond acceptor count 8
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 26.64
% growth inhibition (Hom. pool) 13.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1422863
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:7.44|P-value:5.21E-14|Clearance:2.6||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BUR6(YER159C)|FD-Score:3.81|P-value:6.99E-5|Clearance:0.03||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CCT3(YJL014W)|FD-Score:-6.92|P-value:2.18E-12|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-5.65|P-value:8.14E-9|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC31(YOR257W)|FD-Score:14.9|P-value:2.64E-50|Clearance:6.46||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:HEM4(YOR278W)|FD-Score:3.93|P-value:4.21E-5|Clearance:0.12||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:LIP1(YMR298W)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.02||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MTR2(YKL186C)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NSL1(YPL233W)|FD-Score:-3.23|P-value:6.13E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUP57(YGR119C)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:NUS1(YDL193W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.11||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:OLE1(YGL055W)|FD-Score:4.83|P-value:6.76E-7|Clearance:0.25||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PFS2(YNL317W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.01||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:POP4(YBR257W)|FD-Score:4.04|P-value:2.69E-5|Clearance:0.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RER2(YBR002C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.11||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB3(YIL021W)|FD-Score:-4.07|P-value:2.30E-5|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB5(YBR154C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB7(YDR404C)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPP1(YHR062C)|FD-Score:8.41|P-value:2.04E-17|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMT3(YDR510W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.15||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPC105(YGL093W)|FD-Score:4.58|P-value:2.34E-6|Clearance:0.54||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:8.4|P-value:2.19E-17|Clearance:0.97||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:STT4(YLR305C)|FD-Score:3.13|P-value:8.76E-4|Clearance:0.1||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:TFC1(YBR123C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.09||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:THS1(YIL078W)|FD-Score:3.78|P-value:7.87E-5|Clearance:0.14||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM12(YBR091C)|FD-Score:-3.16|P-value:7.91E-4|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TRE2(YOR256C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.02||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:ASA1(YPR085C)|FD-Score:7.44|P-value:5.21E-14|Clearance:2.6||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BUR6(YER159C)|FD-Score:3.81|P-value:6.99E-5|Clearance:0.03||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CCT3(YJL014W)|FD-Score:-6.92|P-value:2.18E-12|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:-5.65|P-value:8.14E-9|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC31(YOR257W)|FD-Score:14.9|P-value:2.64E-50|Clearance:6.46||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:HEM4(YOR278W)|FD-Score:3.93|P-value:4.21E-5|Clearance:0.12||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:LIP1(YMR298W)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.02||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:MTR2(YKL186C)|FD-Score:-3.37|P-value:3.75E-4|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:NSL1(YPL233W)|FD-Score:-3.23|P-value:6.13E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUP57(YGR119C)|FD-Score:-3.69|P-value:1.14E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:NUS1(YDL193W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.11||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:OLE1(YGL055W)|FD-Score:4.83|P-value:6.76E-7|Clearance:0.25||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PFS2(YNL317W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.01||SGD DESC:Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex Gene:POP4(YBR257W)|FD-Score:4.04|P-value:2.69E-5|Clearance:0.11||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RER2(YBR002C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.11||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPB3(YIL021W)|FD-Score:-4.07|P-value:2.30E-5|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB5(YBR154C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB7(YDR404C)|FD-Score:-3.63|P-value:1.43E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPP1(YHR062C)|FD-Score:8.41|P-value:2.04E-17|Clearance:0.01||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMT3(YDR510W)|FD-Score:3.39|P-value:3.55E-4|Clearance:0.15||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPC105(YGL093W)|FD-Score:4.58|P-value:2.34E-6|Clearance:0.54||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:8.4|P-value:2.19E-17|Clearance:0.97||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:STT4(YLR305C)|FD-Score:3.13|P-value:8.76E-4|Clearance:0.1||SGD DESC:Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization Gene:TFC1(YBR123C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.09||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:THS1(YIL078W)|FD-Score:3.78|P-value:7.87E-5|Clearance:0.14||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM12(YBR091C)|FD-Score:-3.16|P-value:7.91E-4|Clearance:0||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TRE2(YOR256C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.02||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1422863
Download HOP data (tab-delimited text)  (excel)
Gene:ACN9(YDR511W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AEP1(YMR064W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM10(YER087W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APL1(YJR005W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BUD31(YCR063W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CBR1(YIL043C)|FD-Score:8.6|P-value:3.98E-18||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CHO2(YGR157W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLA4(YNL298W)|FD-Score:5.8|P-value:3.31E-9||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.68|P-value:1.46E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ5(YML110C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTL1(YMR180C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:CYS3(YAL012W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAK2(YFL053W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DFG16(YOR030W)|FD-Score:4.43|P-value:4.73E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIA4(YHR011W)|FD-Score:6.59|P-value:2.23E-11||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EGD1(YPL037C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ERV41(YML067C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FYV6(YNL133C)|FD-Score:-4.22|P-value:1.19E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GEP4(YHR100C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLE2(YER107C)|FD-Score:6.26|P-value:1.96E-10||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HSP82(YPL240C)|FD-Score:5.29|P-value:5.98E-8||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HTL1(YCR020W-B)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IRS4(YKR019C)|FD-Score:-5.21|P-value:9.42E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LTE1(YAL024C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MMM1(YLL006W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP20(YDR405W)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL37(YBR268W)|FD-Score:5.26|P-value:7.29E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MUM2(YBR057C)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NBP2(YDR162C)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NRT1(YOR071C)|FD-Score:-5|P-value:2.84E-7||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OPT2(YPR194C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PIB2(YGL023C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:POL32(YJR043C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRD1(YCL057W)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:QCR7(YDR529C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAD18(YCR066W)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD51(YER095W)|FD-Score:7.09|P-value:6.87E-13||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD55(YDR076W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RGC1(YPR115W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.95|P-value:1.80E-19||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT13(YER066W_p)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSC1(YGR056W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SEC66(YBR171W)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLM3(YDL033C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SNF8(YPL002C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOD2(YHR008C)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SRS2(YJL092W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SSA2(YLL024C)|FD-Score:-4.45|P-value:4.27E-6||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:SSN3(YPL042C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STM1(YLR150W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TAT1(YBR069C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TIP41(YPR040W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOK1(YJL093C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TPN1(YGL186C)|FD-Score:-4.96|P-value:3.61E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM1(YDR120C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TSC3(YBR058C-A)|FD-Score:17.7|P-value:2.87E-70||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TYE7(YOR344C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:VPS55(YJR044C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAP1(YML007W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR178W(YBR178W_d)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL010W(YEL010W_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL072C(YGL072C_d)|FD-Score:5.02|P-value:2.65E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL064W(YJL064W_d)|FD-Score:-4.53|P-value:2.96E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJL120W(YJL120W_d)|FD-Score:4.36|P-value:6.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YMR315W(YMR315W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YPK1(YKL126W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL038W-A(YPL038W-A_p)|FD-Score:4.44|P-value:4.53E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZAP1(YJL056C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ACN9(YDR511W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AEP1(YMR064W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIM10(YER087W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APL1(YJR005W)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BUD31(YCR063W)|FD-Score:3.91|P-value:4.62E-5||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CBR1(YIL043C)|FD-Score:8.6|P-value:3.98E-18||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CHO2(YGR157W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLA4(YNL298W)|FD-Score:5.8|P-value:3.31E-9||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG6(YNL041C)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.68|P-value:1.46E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ5(YML110C)|FD-Score:3.76|P-value:8.54E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTL1(YMR180C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:CYS3(YAL012W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAK2(YFL053W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:DFG16(YOR030W)|FD-Score:4.43|P-value:4.73E-6||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIA4(YHR011W)|FD-Score:6.59|P-value:2.23E-11||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EGD1(YPL037C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ERV41(YML067C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein Gene:FYV6(YNL133C)|FD-Score:-4.22|P-value:1.19E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GEP4(YHR100C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLE2(YER107C)|FD-Score:6.26|P-value:1.96E-10||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HSP82(YPL240C)|FD-Score:5.29|P-value:5.98E-8||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HTL1(YCR020W-B)|FD-Score:5.93|P-value:1.51E-9||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IRS4(YKR019C)|FD-Score:-5.21|P-value:9.42E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:LTE1(YAL024C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MMM1(YLL006W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRP20(YDR405W)|FD-Score:6.56|P-value:2.70E-11||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL37(YBR268W)|FD-Score:5.26|P-value:7.29E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MUM2(YBR057C)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex Gene:NBP2(YDR162C)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NRT1(YOR071C)|FD-Score:-5|P-value:2.84E-7||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:OPT2(YPR194C)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PIB2(YGL023C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:POL32(YJR043C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRD1(YCL057W)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:QCR7(YDR529C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAD18(YCR066W)|FD-Score:3.84|P-value:6.04E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD51(YER095W)|FD-Score:7.09|P-value:6.87E-13||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD55(YDR076W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RGC1(YPR115W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Pleckstrin homology domain containing protein; proposed to function as a glycerol channel activator; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; RGC1 has a paralog, ASK10, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.95|P-value:1.80E-19||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRT13(YER066W_p)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:RSC1(YGR056W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SEC66(YBR171W)|FD-Score:5.99|P-value:1.07E-9||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLM3(YDL033C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SNF8(YPL002C)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOD2(YHR008C)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SRS2(YJL092W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SSA2(YLL024C)|FD-Score:-4.45|P-value:4.27E-6||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:SSN3(YPL042C)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:STM1(YLR150W)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TAT1(YBR069C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TIP41(YPR040W)|FD-Score:-3.47|P-value:2.60E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOK1(YJL093C)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TPN1(YGL186C)|FD-Score:-4.96|P-value:3.61E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRM1(YDR120C)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:TSC3(YBR058C-A)|FD-Score:17.7|P-value:2.87E-70||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TYE7(YOR344C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Serine-rich protein that contains a basic-helix-loop-helix (bHLH) DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression Gene:VPS55(YJR044C)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAP1(YML007W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR178W(YBR178W_d)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL010W(YEL010W_d)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL072C(YGL072C_d)|FD-Score:5.02|P-value:2.65E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL064W(YJL064W_d)|FD-Score:-4.53|P-value:2.96E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJL120W(YJL120W_d)|FD-Score:4.36|P-value:6.56E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YMR315W(YMR315W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YPK1(YKL126W)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL038W-A(YPL038W-A_p)|FD-Score:4.44|P-value:4.53E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZAP1(YJL056C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR257W14.902.64E-506.46CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YHR062C8.412.04E-170.01RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR086C8.402.19E-170.97SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YPR085C7.445.21E-142.60ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YGL055W4.836.76E-70.25OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YGL093W4.582.34E-60.54SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YBR257W4.042.69E-50.11POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YOR278W3.934.21E-50.12HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YER159C3.816.99E-50.03BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YIL078W3.787.87E-50.14THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YDL193W3.641.36E-40.11NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YNL317W3.532.08E-40.01PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YOR256C3.522.18E-40.02TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YBR002C3.492.38E-40.11RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDR510W3.393.55E-40.15SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A17.702.87E-70TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YDL020C8.951.80E-19RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YIL043C8.603.98E-18CBR1Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
YER095W7.096.87E-13RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YHR011W6.592.23E-11DIA4Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth
YDR405W6.562.70E-11MRP20Mitochondrial ribosomal protein of the large subunit
YER107C6.261.96E-10GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1
YBR171W5.991.07E-9SEC66Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YCR020W-B5.931.51E-9HTL1Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YNL298W5.803.31E-9CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YPL240C5.295.98E-8HSP82Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
YBR268W5.267.29E-8MRPL37Mitochondrial ribosomal protein of the large subunit
YDR162C5.081.86E-7NBP2Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
YGL072C_d5.022.65E-7YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YCL057W4.904.86E-7PRD1Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_720
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3598.26E-179SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.0869565
0.3541.95E-174SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0289855TSC3-RPN4
0.3531.30E-172SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0735294TSC3-RPN4
0.3332.69E-153SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.113924TSC3-RPN4
0.3223.64E-142SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.078125TSC3-RPN4
0.3101.03E-131SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0493827TSC3-RPN4
0.3009.89E-123SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.109589TSC3-RPN4
0.2963.68E-120SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.0857143
0.2932.92E-117SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.1875TSC3-RPN4
0.2915.22E-116SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.102564
0.2891.07E-113SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.0882353TSC3-RPN4
0.2801.78E-106SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0597015TSC3-RPN4
0.2786.32E-105SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.119048TSC3-RPN4
0.2722.08E-100SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.140625TSC3-RPN4
0.2711.40E-99SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.0746269

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_300r073-00115.04 μM0.452832735780ChemDiv (Drug-like library)282.683043.03806
SGTC_524r073-001027.8 μM0.4444442772106ChemDiv (Drug-like library)284.655861.80807
SGTC_13591582-0056520 nM0.4210533661189ChemDiv (Drug-like library)304.688563.99416
SGTC_9123474-00172.18 μM0.3064523360550ChemDiv (Drug-like library)312.367983.94307
SGTC_8201582-006059.9 μM0.3050853848840ChemDiv (Drug-like library)290.661983.50816
SGTC_9292805-0021398 μM0.2533333098024ChemDiv (Drug-like library)369.39443.07216
SGTC_797k781-0471270 μM0.2523738129ChemDiv (Drug-like library)306.701143.4125RPP1 & pyrimidine depletion
SGTC_10004048-08848.67 μM0.2513040088ChemDiv (Drug-like library)287.249261.60508copper-dependent oxidative stress
SGTC_8151582-002736.9 μM0.2388063128206ChemDiv (Drug-like library)286.24292.82717
SGTC_3650121-005213.6 μM0.2380953101739ChemDiv (Drug-like library)361.564684.2624mitochondrial response to ROS