1187-1332

N'-(3,4-dichlorophenyl)benzohydrazide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_721
Screen concentration 279.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 25730
SMILES C1=CC=C(C=C1)C(=O)NNC2=CC(=C(C=C2)Cl)Cl
Standardized SMILES Clc1ccc(NNC(=O)c2ccccc2)cc1Cl
Molecular weight 281.1373
ALogP 3.97
H-bond donor count 2
H-bond acceptor count 2
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 30.44
% growth inhibition (Hom. pool) 8.42


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25730
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:5.48|P-value:2.15E-8|Clearance:0.09||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT4(YDL143W)|FD-Score:5.93|P-value:1.55E-9|Clearance:0.36||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GCD10(YNL062C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:HEM4(YOR278W)|FD-Score:3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MAS1(YLR163C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.11||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM2(YBL023C)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MSS4(YDR208W)|FD-Score:-3.44|P-value:2.95E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:NOP8(YOL144W)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OLE1(YGL055W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.11||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:5.56|P-value:1.34E-8|Clearance:0.08||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RER2(YBR002C)|FD-Score:4.37|P-value:6.22E-6|Clearance:0.22||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RHO1(YPR165W)|FD-Score:7.38|P-value:8.18E-14|Clearance:1.45||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIM2(YBR192W)|FD-Score:5.39|P-value:3.53E-8|Clearance:0.45||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SPC105(YGL093W)|FD-Score:3.91|P-value:4.71E-5|Clearance:0.24||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TCP1(YDR212W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.16||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOM22(YNL131W)|FD-Score:3.12|P-value:9.15E-4|Clearance:0.13||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:3.13|P-value:8.78E-4|Clearance:0.01||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:USO1(YDL058W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.05||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:UTP15(YMR093W)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:4.94|P-value:3.89E-7|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:4.15|P-value:1.69E-5|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YTH1(YPR107C)|FD-Score:4.8|P-value:7.76E-7|Clearance:0.1||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:BET4(YJL031C)|FD-Score:5.48|P-value:2.15E-8|Clearance:0.09||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT4(YDL143W)|FD-Score:5.93|P-value:1.55E-9|Clearance:0.36||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GCD10(YNL062C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:HEM4(YOR278W)|FD-Score:3.29|P-value:5.04E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MAS1(YLR163C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.11||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM2(YBL023C)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MSS4(YDR208W)|FD-Score:-3.44|P-value:2.95E-4|Clearance:0||SGD DESC:Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation Gene:NOP8(YOL144W)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:OLE1(YGL055W)|FD-Score:3.24|P-value:6.03E-4|Clearance:0.11||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:5.56|P-value:1.34E-8|Clearance:0.08||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RER2(YBR002C)|FD-Score:4.37|P-value:6.22E-6|Clearance:0.22||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RHO1(YPR165W)|FD-Score:7.38|P-value:8.18E-14|Clearance:1.45||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIM2(YBR192W)|FD-Score:5.39|P-value:3.53E-8|Clearance:0.45||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:SPC105(YGL093W)|FD-Score:3.91|P-value:4.71E-5|Clearance:0.24||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TCP1(YDR212W)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.16||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOM22(YNL131W)|FD-Score:3.12|P-value:9.15E-4|Clearance:0.13||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:3.13|P-value:8.78E-4|Clearance:0.01||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:USO1(YDL058W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.05||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:UTP15(YMR093W)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:4.94|P-value:3.89E-7|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:4.15|P-value:1.69E-5|Clearance:0.24||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YTH1(YPR107C)|FD-Score:4.8|P-value:7.76E-7|Clearance:0.1||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25730
Download HOP data (tab-delimited text)  (excel)
Gene:API2(YDR525W_d)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:BCK1(YJL095W)|FD-Score:9.75|P-value:9.44E-23||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BLS1(YLR408C_p)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BNR1(YIL159W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CDA1(YLR307W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:COG6(YNL041C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX9(YDL067C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTK1(YKL139W)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:ERG2(YMR202W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERP1(YAR002C-A)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ERV25(YML012W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FIT1(YDR534C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GAL4(YPL248C)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GYP1(YOR070C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAM1(YJR069C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HER2(YMR293C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HMI1(YOL095C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HSP31(YDR533C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HSP82(YPL240C)|FD-Score:3.76|P-value:8.61E-5||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HST2(YPL015C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRC18(YJL037W)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE1(YNL322C)|FD-Score:4.93|P-value:4.12E-7||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LGE1(YPL055C)|FD-Score:5.21|P-value:9.49E-8||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:4.79|P-value:8.23E-7||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAM33(YIL070C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MKT1(YNL085W)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRPL17(YNL252C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:4.38|P-value:5.96E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS28(YDR337W)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAT4(YMR069W)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:RIM11(YMR139W)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RSM25(YIL093C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SBE22(YHR103W)|FD-Score:6.7|P-value:1.07E-11||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SGE1(YPR198W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLM5(YCR024C)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLT2(YHR030C)|FD-Score:8.42|P-value:1.95E-17||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SYS1(YJL004C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:YCR099C(YCR099C_p)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function Gene:YDL118W(YDL118W_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YGL217C(YGL217C_d)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YNL046W(YNL046W_p)|FD-Score:-3.31|P-value:4.75E-4||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOL085C(YOL085C_d)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YTP1(YNL237W)|FD-Score:5.31|P-value:5.46E-8||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:API2(YDR525W_d)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:BCK1(YJL095W)|FD-Score:9.75|P-value:9.44E-23||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BLS1(YLR408C_p)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BNR1(YIL159W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:CDA1(YLR307W)|FD-Score:-3.12|P-value:9.19E-4||SGD DESC:Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:COG6(YNL041C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX9(YDL067C)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CTK1(YKL139W)|FD-Score:4.86|P-value:5.75E-7||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:ERG2(YMR202W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERP1(YAR002C-A)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:ERV25(YML012W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:FIT1(YDR534C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:GAL4(YPL248C)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GYP1(YOR070C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAM1(YJR069C)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HER2(YMR293C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HMI1(YOL095C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HSP31(YDR533C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:HSP82(YPL240C)|FD-Score:3.76|P-value:8.61E-5||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HST2(YPL015C)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IRC18(YJL037W)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE1(YNL322C)|FD-Score:4.93|P-value:4.12E-7||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LGE1(YPL055C)|FD-Score:5.21|P-value:9.49E-8||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:4.79|P-value:8.23E-7||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAM33(YIL070C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MKT1(YNL085W)|FD-Score:-4.01|P-value:3.06E-5||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRPL17(YNL252C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL7(YDR237W)|FD-Score:4.38|P-value:5.96E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:MRPS28(YDR337W)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAT4(YMR069W)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:RIM11(YMR139W)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress Gene:RSM25(YIL093C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SBE22(YHR103W)|FD-Score:6.7|P-value:1.07E-11||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SGE1(YPR198W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLM5(YCR024C)|FD-Score:4.37|P-value:6.11E-6||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SLT2(YHR030C)|FD-Score:8.42|P-value:1.95E-17||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SYS1(YJL004C)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:YCR099C(YCR099C_p)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function Gene:YDL118W(YDL118W_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YGL217C(YGL217C_d)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.32|P-value:7.96E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YNL046W(YNL046W_p)|FD-Score:-3.31|P-value:4.75E-4||SGD DESC:Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOL085C(YOL085C_d)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YTP1(YNL237W)|FD-Score:5.31|P-value:5.46E-8||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W7.388.18E-141.45RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDL143W5.931.55E-90.36CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YBL105C5.561.34E-80.08PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YJL031C5.482.15E-80.09BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YBR192W5.393.53E-80.45RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YDL196W_d4.943.89E-70.14YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YPR107C4.807.76E-70.10YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YMR290W-A_d4.701.30E-60.33YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YBR002C4.376.22E-60.22RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YJL086C_d4.151.69E-50.24YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YGL093W3.914.71E-50.24SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YDR212W3.671.22E-40.16TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YLR163C3.512.26E-40.11MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YOL144W3.393.45E-40.10NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YOR278W3.295.04E-40.00HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL095W9.759.44E-23BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YHR030C8.421.95E-17SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YHR103W6.701.07E-11SBE22Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
YNL237W5.315.46E-8YTP1Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
YPL055C5.219.49E-8LGE1Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
YNL322C4.934.12E-7KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YKL139W4.865.75E-7CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YLR239C4.798.23E-7LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YML012W4.691.39E-6ERV25Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport
YDR237W4.385.96E-6MRPL7Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
YCR024C4.376.11E-6SLM5Mitochondrial asparaginyl-tRNA synthetase
YPL248C4.347.04E-6GAL4DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p
YKL068W-A_p4.327.96E-6YKL068W-A_pPutative protein of unknown function; identified by homology to Ashbya gossypii
YDL067C4.151.66E-5COX9Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YOL095C4.082.26E-5HMI1Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD

GO enrichment analysis for SGTC_721
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3524.39E-172SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.137931cell wall signaling
0.3114.69E-133SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0823529cell wall signaling
0.2911.97E-115SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.12766cell wall signaling
0.2834.21E-109SGTC_31229126309 49.5 μMChembridge (Drug-like library)175912060.0724638cell wall signaling
0.2791.11E-105SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0821918cell wall signaling
0.2783.09E-105SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0847458cell wall signaling
0.2739.57E-102SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.23913cell wall signaling
0.2554.46E-88SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.1875cell wall signaling
0.2541.91E-87SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.15cell wall
0.2374.95E-76SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.105263cell wall signaling
0.2367.27E-76SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.127273cell wall signaling
0.2358.53E-75SGTC_500h-89 112.0 μMICCB bioactive library4492410.0810811cell wall signaling
0.2301.29E-71SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.264151Golgi
0.2282.29E-70SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0632911cell wall
0.2247.52E-68SGTC_29127964298 10.9 μMChembridge (Drug-like library)29758500.192982cell wall

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12971068-011114.1 μM0.454545767057ChemDiv (Drug-like library)312.214244.33712
SGTC_12961068-010211.9 μM0.434783767053ChemDiv (Drug-like library)310.175224.02712
SGTC_1270180-033359.13 μM0.425532771601ChemDiv (Drug-like library)324.158743.71113mitochondrial response to ROS
SGTC_2929901334824.6 μM0.4042552995952Chembridge (Drug-like library)310.175223.99912
SGTC_3006907542471.43 μM0.47465181Chembridge (Drug-like library)334.160162.8314
SGTC_8480368-008985.6 μM0.391304762535ChemDiv (Drug-like library)267.110722.79212iron homeostasis
SGTC_3118912425049.47 μM0.381504786Chembridge (Drug-like library)317.16944.12912
SGTC_10154130-452582 μM0.3653851363237ChemDiv (Drug-like library)392.834983.8032360S ribosome export
SGTC_344k064-00356.43 μM0.3636365088750ChemDiv (Drug-like library)339.217986.2321260S ribosome export
SGTC_9061165-0455526 μM0.3555563252859ChemDiv (Drug-like library)304.364141.90224